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The map label for this gene is pcrA

Identifier: 15835512

GI number: 15835512

Start: 1040188

End: 1042092

Strand: Direct

Name: pcrA

Synonym: TC0898

Alternate gene names: 15835512

Gene position: 1040188-1042092 (Clockwise)

Preceding gene: 15835509

Following gene: 15835513

Centisome position: 96.95

GC content: 40.05

Gene sequence:

>1905_bases
ATGCTTACATCCGAATTAAATGCAGCACAAGTTACCGCCGTCACTGCGCCATTACAACCAGTCCTTGTTTTAGCTGGAGC
TGGAGCTGGAAAAACTCGAGTGGTTTCCCATCGAATTCTTTACCTAATCGAAGAGGCTCAACTCGATCCATCGCAAATTT
TAGCAATAACATTCACTAACAAAGCTGCAAAAGAATTAAACGAACGAGTCCTAACTCAATGCAACTTTCCAGACTATCGA
GGCATACCTATGGTGAGCACCTTTCATAGCTTAGGTGTTTATATTCTTCGCCGATCAATCCAACTCCTAGATAGACAATC
AAATTTTGCTATTTATGATCAAAGCGATTCTGAAAAATTAATCAAGCAGTGCTTACGTAAACTCAATCTCGATAATAAAC
TTTGCAATGCAATGCAGTTTACTATCTCTCAAGCAAAAAATCGTCTACAAGATCCAGAAGATCTAGATTCTAGAGAATAT
CCAGATCCTGCCCGTGCAGTTTATACTGAATACCAAGAACAGTTACGCGCTGCGAATGCTCTGGACTTTGATGATCTCTT
ATTTTTGACAGAAAAATTACTCCGCATCCCCGAAATCCAACAGGAATATTCCAACCTCTGGAAAGCCTTATTAATAGATG
AGTATCAAGACACTAATCATGCGCAATACCTCATCGCTAAACGTCTTGCAGCCTCTCACAACAATATTTTTGTCGTTGGA
GATCCAGATCAATCCATATATTCCTGGCGAGGTGCTAACATCTCTAATATTTTAAATTTTGAACAAGATTATCCAAAGGC
TTTGGTCGTTCGTCTGGAAGAAAATTACCGTTCCTGCGGAACTATTTTAGAGGCAGCAAATGCTCTCATTCAAAATAATT
CTGCTCGTTTAGATAAAACTTTGCGTAGTGTGAAAGGCCCTGGAGATAAAATTTTCTGTTTCACAGGGAAAAATGACCGC
GACGAAGCGGAACAAGTATTAGAAGAAATTTCCAATCTTCACTCATACAAAGATATTCCTCTCTCTCATATCTGCATTTT
GTATCGAACAAATTTTCAATCGCAATCATTCGAAGCAGCTCTTCTCAAGCGTGGTTATCCCTATGAAATTATAGGAGGCA
TTTCCTTCTACAAACGCAAAGAAATCCAAGATATTCTCGCTTTCTTGCGTCTCTTTTCTAACAACTACGATATGGCCGCT
TTTGAGCGCACTATCGGACTTAAGAAATGCGGTATTGGCGCTACCACCCTATCGTCTCTCATGAGTTATGCGAAAAGTAT
GGACCTTCCTATCCTGCAAGCATGCTGGGATGTCCTAGAGAAAAAACCCATCCGTTTAACAAAAAAACAACAACAGGGGC
TTTTCTCTTATCTTACGCATTTCCATCAGATGGAAAAACTTTATGGAAACTGCGATCTTCATGAATTTATTAATGAGACT
ATTCGCATTACGGATTACCTCGCCATTCTCAAAGAGGACCCAGAAACTTATGACGATAGAAAGAATAACCTAGAACAGTT
ACTAGCAGAAACACAAATATGGGGCAAAAGCAGTGAGAACCTCCCGAACTTTTTAGAAGATTTAGCTCTAAAAAGTTCAG
CAGATGAAACTGCAAGTTCTCATGATCGTCTTAAACTCATGACCATCCACAATAGCAAGGGGTTAGAATTTCCTGTAGTC
TTCTTAGTTGGACTCGAAGAAAACCTACTCCCTCATGCAAATTCCAAAGGCATGCACGAAAATATTGAAGAGGAACGAAG
ATTATGTTACGTGGGAATTACCCGAGCACAAGAGTACCTCTACTTATCTCGAGCAAAAACCCGATTCCTTTGGGGGACGG
AACGCACCATGGTCCCAAGCAGATTTATTAGCGAGCTGCCTAGGGCTCTTTTAAAATTTGTTTAA

Upstream 100 bases:

>100_bases
ATAGTAAAAGCCTCATGCATATCGCTCTCCTTAATCTAATGCATAGAGTATATGCATTCTCGAAAATTTTCTTCGAACCT
GAAAAATATATTACGTACGT

Downstream 100 bases:

>100_bases
CCTATGTTGCATCAGCATCAAACAGCATCTGTAGCTCTTTGTCCTGCTTTACATATTCAACAAAGTTTGGACATGCTACA
GATGCCGGTTGCTGAACTCT

Product: ATP-dependent helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MLTSELNAAQVTAVTAPLQPVLVLAGAGAGKTRVVSHRILYLIEEAQLDPSQILAITFTNKAAKELNERVLTQCNFPDYR
GIPMVSTFHSLGVYILRRSIQLLDRQSNFAIYDQSDSEKLIKQCLRKLNLDNKLCNAMQFTISQAKNRLQDPEDLDSREY
PDPARAVYTEYQEQLRAANALDFDDLLFLTEKLLRIPEIQQEYSNLWKALLIDEYQDTNHAQYLIAKRLAASHNNIFVVG
DPDQSIYSWRGANISNILNFEQDYPKALVVRLEENYRSCGTILEAANALIQNNSARLDKTLRSVKGPGDKIFCFTGKNDR
DEAEQVLEEISNLHSYKDIPLSHICILYRTNFQSQSFEAALLKRGYPYEIIGGISFYKRKEIQDILAFLRLFSNNYDMAA
FERTIGLKKCGIGATTLSSLMSYAKSMDLPILQACWDVLEKKPIRLTKKQQQGLFSYLTHFHQMEKLYGNCDLHEFINET
IRITDYLAILKEDPETYDDRKNNLEQLLAETQIWGKSSENLPNFLEDLALKSSADETASSHDRLKLMTIHNSKGLEFPVV
FLVGLEENLLPHANSKGMHENIEEERRLCYVGITRAQEYLYLSRAKTRFLWGTERTMVPSRFISELPRALLKFV

Sequences:

>Translated_634_residues
MLTSELNAAQVTAVTAPLQPVLVLAGAGAGKTRVVSHRILYLIEEAQLDPSQILAITFTNKAAKELNERVLTQCNFPDYR
GIPMVSTFHSLGVYILRRSIQLLDRQSNFAIYDQSDSEKLIKQCLRKLNLDNKLCNAMQFTISQAKNRLQDPEDLDSREY
PDPARAVYTEYQEQLRAANALDFDDLLFLTEKLLRIPEIQQEYSNLWKALLIDEYQDTNHAQYLIAKRLAASHNNIFVVG
DPDQSIYSWRGANISNILNFEQDYPKALVVRLEENYRSCGTILEAANALIQNNSARLDKTLRSVKGPGDKIFCFTGKNDR
DEAEQVLEEISNLHSYKDIPLSHICILYRTNFQSQSFEAALLKRGYPYEIIGGISFYKRKEIQDILAFLRLFSNNYDMAA
FERTIGLKKCGIGATTLSSLMSYAKSMDLPILQACWDVLEKKPIRLTKKQQQGLFSYLTHFHQMEKLYGNCDLHEFINET
IRITDYLAILKEDPETYDDRKNNLEQLLAETQIWGKSSENLPNFLEDLALKSSADETASSHDRLKLMTIHNSKGLEFPVV
FLVGLEENLLPHANSKGMHENIEEERRLCYVGITRAQEYLYLSRAKTRFLWGTERTMVPSRFISELPRALLKFV
>Mature_634_residues
MLTSELNAAQVTAVTAPLQPVLVLAGAGAGKTRVVSHRILYLIEEAQLDPSQILAITFTNKAAKELNERVLTQCNFPDYR
GIPMVSTFHSLGVYILRRSIQLLDRQSNFAIYDQSDSEKLIKQCLRKLNLDNKLCNAMQFTISQAKNRLQDPEDLDSREY
PDPARAVYTEYQEQLRAANALDFDDLLFLTEKLLRIPEIQQEYSNLWKALLIDEYQDTNHAQYLIAKRLAASHNNIFVVG
DPDQSIYSWRGANISNILNFEQDYPKALVVRLEENYRSCGTILEAANALIQNNSARLDKTLRSVKGPGDKIFCFTGKNDR
DEAEQVLEEISNLHSYKDIPLSHICILYRTNFQSQSFEAALLKRGYPYEIIGGISFYKRKEIQDILAFLRLFSNNYDMAA
FERTIGLKKCGIGATTLSSLMSYAKSMDLPILQACWDVLEKKPIRLTKKQQQGLFSYLTHFHQMEKLYGNCDLHEFINET
IRITDYLAILKEDPETYDDRKNNLEQLLAETQIWGKSSENLPNFLEDLALKSSADETASSHDRLKLMTIHNSKGLEFPVV
FLVGLEENLLPHANSKGMHENIEEERRLCYVGITRAQEYLYLSRAKTRFLWGTERTMVPSRFISELPRALLKFV

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=640, Percent_Identity=38.59375, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI48994965, Length=638, Percent_Identity=38.5579937304075, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787196, Length=449, Percent_Identity=26.7260579064588, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322369, Length=764, Percent_Identity=28.5340314136126, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324477, Length=645, Percent_Identity=23.2558139534884, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 72680; Mature: 72680

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSELNAAQVTAVTAPLQPVLVLAGAGAGKTRVVSHRILYLIEEAQLDPSQILAITFTN
CCCCCCCCHHEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECC
KAAKELNERVLTQCNFPDYRGIPMVSTFHSLGVYILRRSIQLLDRQSNFAIYDQSDSEKL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
IKQCLRKLNLDNKLCNAMQFTISQAKNRLQDPEDLDSREYPDPARAVYTEYQEQLRAANA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
LDFDDLLFLTEKLLRIPEIQQEYSNLWKALLIDEYQDTNHAQYLIAKRLAASHNNIFVVG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEE
DPDQSIYSWRGANISNILNFEQDYPKALVVRLEENYRSCGTILEAANALIQNNSARLDKT
CCCCHHHHCCCCCHHHHHCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LRSVKGPGDKIFCFTGKNDRDEAEQVLEEISNLHSYKDIPLSHICILYRTNFQSQSFEAA
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCCHHHHH
LLKRGYPYEIIGGISFYKRKEIQDILAFLRLFSNNYDMAAFERTIGLKKCGIGATTLSSL
HHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHCCCCHHHHHHH
MSYAKSMDLPILQACWDVLEKKPIRLTKKQQQGLFSYLTHFHQMEKLYGNCDLHEFINET
HHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IRITDYLAILKEDPETYDDRKNNLEQLLAETQIWGKSSENLPNFLEDLALKSSADETASS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
HDRLKLMTIHNSKGLEFPVVFLVGLEENLLPHANSKGMHENIEEERRLCYVGITRAQEYL
CCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCEEEEEHHHHHHHH
YLSRAKTRFLWGTERTMVPSRFISELPRALLKFV
HHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTSELNAAQVTAVTAPLQPVLVLAGAGAGKTRVVSHRILYLIEEAQLDPSQILAITFTN
CCCCCCCCHHEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECC
KAAKELNERVLTQCNFPDYRGIPMVSTFHSLGVYILRRSIQLLDRQSNFAIYDQSDSEKL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
IKQCLRKLNLDNKLCNAMQFTISQAKNRLQDPEDLDSREYPDPARAVYTEYQEQLRAANA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
LDFDDLLFLTEKLLRIPEIQQEYSNLWKALLIDEYQDTNHAQYLIAKRLAASHNNIFVVG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEE
DPDQSIYSWRGANISNILNFEQDYPKALVVRLEENYRSCGTILEAANALIQNNSARLDKT
CCCCHHHHCCCCCHHHHHCCCCCCCHHHHHEEHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LRSVKGPGDKIFCFTGKNDRDEAEQVLEEISNLHSYKDIPLSHICILYRTNFQSQSFEAA
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCCHHHHH
LLKRGYPYEIIGGISFYKRKEIQDILAFLRLFSNNYDMAAFERTIGLKKCGIGATTLSSL
HHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHCCCCHHHHHHH
MSYAKSMDLPILQACWDVLEKKPIRLTKKQQQGLFSYLTHFHQMEKLYGNCDLHEFINET
HHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IRITDYLAILKEDPETYDDRKNNLEQLLAETQIWGKSSENLPNFLEDLALKSSADETASS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
HDRLKLMTIHNSKGLEFPVVFLVGLEENLLPHANSKGMHENIEEERRLCYVGITRAQEYL
CCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCEEEEEHHHHHHHH
YLSRAKTRFLWGTERTMVPSRFISELPRALLKFV
HHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]