Click here to switch to the map view.

The map label for this gene is eno

Identifier: 15835490

GI number: 15835490

Start: 1014914

End: 1016188

Strand: Direct

Name: eno

Synonym: TC0876

Alternate gene names: 15835490

Gene position: 1014914-1016188 (Clockwise)

Preceding gene: 15835489

Following gene: 15835491

Centisome position: 94.59

GC content: 43.29

Gene sequence:

>1275_bases
ATGTTTGATGTTGTCATCTCCGATATAGAAGCTAGAGAAATTTTAGATTCCCGAGGGTATCCTACCTTGTATGTTAAAGT
CATTACTAATACAGGAATCTTTGGAGAAGCTTGTGTTCCTTCTGGAGCATCTACAGGCATTAAAGAAGCTTTAGAACTTC
GTGACCAAGATCCTAAACGCTATCAAGGAAAAGGAGTTCTACAAGCAGTCGCTAATGTGGAAAAAGTTCTATTACCTGCC
CTACAAGGGTTCAGTGTTTTTGATCAAATCACAGCAGACGCTATTATGATCGATGCTGATGGCACCTCTAACAAGGAAAA
ATTAGGCGCCAATGCGATTCTTGGGGTTTCTTTAGCATTAGCAAAGGCTGCTGCTGCAACTTTAGAGAGACCTTTATACC
GGTATTTGGGAGGAGCTTTCTCGCATGTTCTCCCTTGCCCAATGATGAACCTCATTAACGGTGGCATGCATGCAACAAAT
GGCCTCCAATTTCAAGAATTTATGATCCGTCCTATTAGCGCTCCTTCCCTAACAGAAGCTGTGCGCATGGGAGCAGAAGT
TTTTCACACTTTGAAAAAAATCTTACAAAATCGGCAACTTTCTACAGGAGTCGGAGATGAAGGCGGATTCGCTCCCCAAC
TCGCTTCCAATTCAGAGGCGCTTGACCTTCTCTTGGCTGCTATTGAAAAGGCTGGCTTTATCCCTGGAGAGGATATTTCT
TTAGCTCTTGATTGCGCAGCTTCCTCGTTTTACAATACTCAGGATAAAACTTATGATGGCAAGTCCGCTGCAGATCAAGT
TGCTGTACTCACAGAATTATGCGATCGCTATCCTATAGATTCTATCGAAGATGGCCTTGCTGAGGAAGATTTTGAAGGCT
GGAAGCTTTTATCAGAAACTTTAGGAGATCGCATTCAATTAGTAGGAGATGATTTATTTGTCACGAACTCTGCTTTGATT
GCCGAAGGAATTGCGCAAGGCCTAGCTAATGCCGTTCTTATCAAACCCAATCAGATAGGGACGCTTACAGAAACAGCGGA
GGCTATCCGCTTAGCAACTACGCAGGGCTATGCGACCATCCTATCTCACAGATCAGGAGAAACAGAAGACACCACAATCG
CAGATCTTGCGGTTGCCTTTAACACAGGGCAAATTAAAACAGGCTCTCTCTCTCGTTCCGAGCGTATTGCTAAATACAAC
CGCTTGATTGCTATTGAAGAAGAAATAGGCCCAGAAGCTGTATTTCAAGATTCCAATCCTTTTTCTAAAGCATAA

Upstream 100 bases:

>100_bases
TCCGGGCTTCTCTTTATTTAGTACCGTAAGATTCTTATCGGAGGAAACAAATAAAACGAGCGAACCGCTTTCATTAACCA
TTCTAGACAAGGATCTTGTT

Downstream 100 bases:

>100_bases
CTAGGTTGATGAGTTAAGAAGGGATTTTTATAAAGATCCCTTCTCCTCTTTGCTTGTAGATTTTTTTTCAGGAGAGTATG
TAAAGCTTTATCCTGTTCCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MFDVVISDIEAREILDSRGYPTLYVKVITNTGIFGEACVPSGASTGIKEALELRDQDPKRYQGKGVLQAVANVEKVLLPA
LQGFSVFDQITADAIMIDADGTSNKEKLGANAILGVSLALAKAAAATLERPLYRYLGGAFSHVLPCPMMNLINGGMHATN
GLQFQEFMIRPISAPSLTEAVRMGAEVFHTLKKILQNRQLSTGVGDEGGFAPQLASNSEALDLLLAAIEKAGFIPGEDIS
LALDCAASSFYNTQDKTYDGKSAADQVAVLTELCDRYPIDSIEDGLAEEDFEGWKLLSETLGDRIQLVGDDLFVTNSALI
AEGIAQGLANAVLIKPNQIGTLTETAEAIRLATTQGYATILSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYN
RLIAIEEEIGPEAVFQDSNPFSKA

Sequences:

>Translated_424_residues
MFDVVISDIEAREILDSRGYPTLYVKVITNTGIFGEACVPSGASTGIKEALELRDQDPKRYQGKGVLQAVANVEKVLLPA
LQGFSVFDQITADAIMIDADGTSNKEKLGANAILGVSLALAKAAAATLERPLYRYLGGAFSHVLPCPMMNLINGGMHATN
GLQFQEFMIRPISAPSLTEAVRMGAEVFHTLKKILQNRQLSTGVGDEGGFAPQLASNSEALDLLLAAIEKAGFIPGEDIS
LALDCAASSFYNTQDKTYDGKSAADQVAVLTELCDRYPIDSIEDGLAEEDFEGWKLLSETLGDRIQLVGDDLFVTNSALI
AEGIAQGLANAVLIKPNQIGTLTETAEAIRLATTQGYATILSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYN
RLIAIEEEIGPEAVFQDSNPFSKA
>Mature_424_residues
MFDVVISDIEAREILDSRGYPTLYVKVITNTGIFGEACVPSGASTGIKEALELRDQDPKRYQGKGVLQAVANVEKVLLPA
LQGFSVFDQITADAIMIDADGTSNKEKLGANAILGVSLALAKAAAATLERPLYRYLGGAFSHVLPCPMMNLINGGMHATN
GLQFQEFMIRPISAPSLTEAVRMGAEVFHTLKKILQNRQLSTGVGDEGGFAPQLASNSEALDLLLAAIEKAGFIPGEDIS
LALDCAASSFYNTQDKTYDGKSAADQVAVLTELCDRYPIDSIEDGLAEEDFEGWKLLSETLGDRIQLVGDDLFVTNSALI
AEGIAQGLANAVLIKPNQIGTLTETAEAIRLATTQGYATILSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYN
RLIAIEEEIGPEAVFQDSNPFSKA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=50.6976744186047, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=48.7414187643021, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI301897477, Length=440, Percent_Identity=49.7727272727273, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI301897469, Length=440, Percent_Identity=49.7727272727273, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI301897479, Length=437, Percent_Identity=44.8512585812357, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=27.893175074184, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=27.893175074184, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=27.893175074184, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI310129182, Length=125, Percent_Identity=33.6, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310110045, Length=125, Percent_Identity=33.6, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310120572, Length=125, Percent_Identity=33.6, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789141, Length=420, Percent_Identity=54.047619047619, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=50.2314814814815, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=50.2314814814815, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.3541666666667, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=47.8260869565217, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323985, Length=441, Percent_Identity=45.3514739229025, Blast_Score=340, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6324974, Length=441, Percent_Identity=45.3514739229025, Blast_Score=339, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6324969, Length=441, Percent_Identity=45.3514739229025, Blast_Score=339, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=47.1395881006865, Blast_Score=336, Evalue=5e-93,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=50, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=50, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=50, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=50, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=49.8839907192575, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=49.8839907192575, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_CHLMU (Q9PJF3)

Other databases:

- EMBL:   AE002160
- PIR:   E81654
- RefSeq:   NP_297249.1
- ProteinModelPortal:   Q9PJF3
- SMR:   Q9PJF3
- GeneID:   1246244
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0876
- TIGR:   TC_0876
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   CMUR243161:TC_0876-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45299; Mature: 45299

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 207-207 ACT_SITE 335-335 BINDING 157-157 BINDING 166-166 BINDING 283-283 BINDING 310-310 BINDING 335-335 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVVISDIEAREILDSRGYPTLYVKVITNTGIFGEACVPSGASTGIKEALELRDQDPKR
CCCEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHH
YQGKGVLQAVANVEKVLLPALQGFSVFDQITADAIMIDADGTSNKEKLGANAILGVSLAL
HCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEECCCCCCHHHHCCHHHHHHHHHH
AKAAAATLERPLYRYLGGAFSHVLPCPMMNLINGGMHATNGLQFQEFMIRPISAPSLTEA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHH
VRMGAEVFHTLKKILQNRQLSTGVGDEGGFAPQLASNSEALDLLLAAIEKAGFIPGEDIS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
LALDCAASSFYNTQDKTYDGKSAADQVAVLTELCDRYPIDSIEDGLAEEDFEGWKLLSET
EEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHH
LGDRIQLVGDDLFVTNSALIAEGIAQGLANAVLIKPNQIGTLTETAEAIRLATTQGYATI
HCCCEEEECCCEEEECHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHCCCHHHH
LSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYNRLIAIEEEIGPEAVFQDSNP
HHCCCCCCCCCHHHHEEEEECCCCEECCCCCHHHHHHHHHHEEEEHHHCCCCCEECCCCC
FSKA
CCCC
>Mature Secondary Structure
MFDVVISDIEAREILDSRGYPTLYVKVITNTGIFGEACVPSGASTGIKEALELRDQDPKR
CCCEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHH
YQGKGVLQAVANVEKVLLPALQGFSVFDQITADAIMIDADGTSNKEKLGANAILGVSLAL
HCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEECCCCCCHHHHCCHHHHHHHHHH
AKAAAATLERPLYRYLGGAFSHVLPCPMMNLINGGMHATNGLQFQEFMIRPISAPSLTEA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHH
VRMGAEVFHTLKKILQNRQLSTGVGDEGGFAPQLASNSEALDLLLAAIEKAGFIPGEDIS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
LALDCAASSFYNTQDKTYDGKSAADQVAVLTELCDRYPIDSIEDGLAEEDFEGWKLLSET
EEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHH
LGDRIQLVGDDLFVTNSALIAEGIAQGLANAVLIKPNQIGTLTETAEAIRLATTQGYATI
HCCCEEEECCCEEEECHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHCCCHHHH
LSHRSGETEDTTIADLAVAFNTGQIKTGSLSRSERIAKYNRLIAIEEEIGPEAVFQDSNP
HHCCCCCCCCCHHHHEEEEECCCCEECCCCCHHHHHHHHHHEEEEHHHCCCCCEECCCCC
FSKA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935