Click here to switch to the map view.

The map label for this gene is trpS

Identifier: 15835488

GI number: 15835488

Start: 1011689

End: 1012729

Strand: Direct

Name: trpS

Synonym: TC0874

Alternate gene names: 15835488

Gene position: 1011689-1012729 (Clockwise)

Preceding gene: 15835487

Following gene: 15835489

Centisome position: 94.29

GC content: 41.79

Gene sequence:

>1041_bases
ATGAATAAAAAACGAGTTCTTACTGGAGATCGTCCTACAGGGAAACTTCATCTGGGGCATTGGATTGGCTCTATTATGAA
TCGCCTGCAGCTACAAAACGATCCTCGCTATGATTGTTTCTTCATCATAGCAGATTTGCATACGCTAACCACTAAAACAC
GAAAAGAAGAAGTTCTTCATATCGATAGCCATATTTATGATGTCCTTGCTGATTGGTTAAGCGTAGGCATCGATCCAGAA
AAGTCTGCTATCTACCTGCAATCAGCTATTCCCGAGATCTATGAATTAAATCTCCTCTTTTCGATGCTGACTCCCTTGAA
TCACATCATGGGTATTCCCAGCATTAAAGAGATGGCTCGCAATGCTTCTATCAATGAAGAAAGTCTTTCCCATGGGCTTA
TAGGTTATCCTGTATTGCAAAGCGCCGACATTTTACTTGCTAAAGCGCATCTTGTTCCTGTTGGTAAAGATAACGAAGCT
CACGTAGAATTGACTCGTGATATCGCAAAAACATTCAATCGGCTGTACGGATCCGTTTTCCCAGAGCCCGATATCTTACA
AGGAGAGCTAACCTCTCTGGTAGGCACGAACGGTCAAGGAAAGATGAGTAAATCCGCTAATAATGCCATTTATCTTTCGG
ATGATGAGAAGACGGTACAAGAAAAAATACGAAAAATGTACACAGATCCTAACCGAGTTCATGCAACGACTCCCGGAAGA
GTAGAAGGCAATCCCTTATTTATCTACCATGATCTTTTTAATCCTCACAAAGAAGAGGTGGAAGAGTTTAAAACTCGTTA
CCGCCAAGGATGCATTAAAGATGTGGAAGTAAAAGCCCGCTTAGCAGAAGAGATTAACCTTTTCCTCAATCCTTTCCGAG
AAAAACGCAGCGAATTCGTCGCTCAACCTAAGATTCTTGAAGAGGCTTTACAGAAAGGTACGGAAAAAATGCGATCAGTA
GCACGGGAAACTATGGAAGAAGTCCATAACAACCTAGGATTAAGCCGCAAGTGGCGCTCTATTCTAGCTTCTTCTAAATG
A

Upstream 100 bases:

>100_bases
CTTTGGGGGCTTATCTTTGAATAATCGGCAAGTTAAGAATTAAAGAAACAGCAAGGAACGAGGTTTCTTCCCAAATTGGG
GCCCGCCTCGTAAATCGACC

Downstream 100 bases:

>100_bases
CGCATTACCATAATCCTTTGAATGGGAGGAAAGTTCTGAATCAGCAGTTTGTATTACAAGCCCCTTTCCTCCCTTGTGGA
GATCAGCCTGAGGCAATTCG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNKKRVLTGDRPTGKLHLGHWIGSIMNRLQLQNDPRYDCFFIIADLHTLTTKTRKEEVLHIDSHIYDVLADWLSVGIDPE
KSAIYLQSAIPEIYELNLLFSMLTPLNHIMGIPSIKEMARNASINEESLSHGLIGYPVLQSADILLAKAHLVPVGKDNEA
HVELTRDIAKTFNRLYGSVFPEPDILQGELTSLVGTNGQGKMSKSANNAIYLSDDEKTVQEKIRKMYTDPNRVHATTPGR
VEGNPLFIYHDLFNPHKEEVEEFKTRYRQGCIKDVEVKARLAEEINLFLNPFREKRSEFVAQPKILEEALQKGTEKMRSV
ARETMEEVHNNLGLSRKWRSILASSK

Sequences:

>Translated_346_residues
MNKKRVLTGDRPTGKLHLGHWIGSIMNRLQLQNDPRYDCFFIIADLHTLTTKTRKEEVLHIDSHIYDVLADWLSVGIDPE
KSAIYLQSAIPEIYELNLLFSMLTPLNHIMGIPSIKEMARNASINEESLSHGLIGYPVLQSADILLAKAHLVPVGKDNEA
HVELTRDIAKTFNRLYGSVFPEPDILQGELTSLVGTNGQGKMSKSANNAIYLSDDEKTVQEKIRKMYTDPNRVHATTPGR
VEGNPLFIYHDLFNPHKEEVEEFKTRYRQGCIKDVEVKARLAEEINLFLNPFREKRSEFVAQPKILEEALQKGTEKMRSV
ARETMEEVHNNLGLSRKWRSILASSK
>Mature_346_residues
MNKKRVLTGDRPTGKLHLGHWIGSIMNRLQLQNDPRYDCFFIIADLHTLTTKTRKEEVLHIDSHIYDVLADWLSVGIDPE
KSAIYLQSAIPEIYELNLLFSMLTPLNHIMGIPSIKEMARNASINEESLSHGLIGYPVLQSADILLAKAHLVPVGKDNEA
HVELTRDIAKTFNRLYGSVFPEPDILQGELTSLVGTNGQGKMSKSANNAIYLSDDEKTVQEKIRKMYTDPNRVHATTPGR
VEGNPLFIYHDLFNPHKEEVEEFKTRYRQGCIKDVEVKARLAEEINLFLNPFREKRSEFVAQPKILEEALQKGTEKMRSV
ARETMEEVHNNLGLSRKWRSILASSK

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=343, Percent_Identity=30.6122448979592, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI41352700, Length=184, Percent_Identity=38.0434782608696, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1789786, Length=349, Percent_Identity=32.0916905444126, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71982800, Length=338, Percent_Identity=27.810650887574, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982793, Length=338, Percent_Identity=27.810650887574, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320474, Length=351, Percent_Identity=31.0541310541311, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24666151, Length=339, Percent_Identity=33.0383480825959, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_CHLMU (Q9PJF5)

Other databases:

- EMBL:   AE002160
- PIR:   C81654
- RefSeq:   NP_297247.1
- ProteinModelPortal:   Q9PJF5
- SMR:   Q9PJF5
- GeneID:   1246242
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0874
- TIGR:   TC_0874
- HOGENOM:   HBG293263
- OMA:   DNESHIE
- ProtClustDB:   PRK00927
- BioCyc:   CMUR243161:TC_0874-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 39413; Mature: 39413

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 204-204

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKRVLTGDRPTGKLHLGHWIGSIMNRLQLQNDPRYDCFFIIADLHTLTTKTRKEEVLH
CCCCCEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHH
IDSHIYDVLADWLSVGIDPEKSAIYLQSAIPEIYELNLLFSMLTPLNHIMGIPSIKEMAR
HHHHHHHHHHHHHHCCCCCCHHEEEHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
NASINEESLSHGLIGYPVLQSADILLAKAHLVPVGKDNEAHVELTRDIAKTFNRLYGSVF
CCCCCHHHHHCCCCCCHHHHCCHHHEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
PEPDILQGELTSLVGTNGQGKMSKSANNAIYLSDDEKTVQEKIRKMYTDPNRVHATTPGR
CCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCCCC
VEGNPLFIYHDLFNPHKEEVEEFKTRYRQGCIKDVEVKARLAEEINLFLNPFREKRSEFV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQPKILEEALQKGTEKMRSVARETMEEVHNNLGLSRKWRSILASSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNKKRVLTGDRPTGKLHLGHWIGSIMNRLQLQNDPRYDCFFIIADLHTLTTKTRKEEVLH
CCCCCEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHH
IDSHIYDVLADWLSVGIDPEKSAIYLQSAIPEIYELNLLFSMLTPLNHIMGIPSIKEMAR
HHHHHHHHHHHHHHCCCCCCHHEEEHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
NASINEESLSHGLIGYPVLQSADILLAKAHLVPVGKDNEAHVELTRDIAKTFNRLYGSVF
CCCCCHHHHHCCCCCCHHHHCCHHHEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
PEPDILQGELTSLVGTNGQGKMSKSANNAIYLSDDEKTVQEKIRKMYTDPNRVHATTPGR
CCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCCCC
VEGNPLFIYHDLFNPHKEEVEEFKTRYRQGCIKDVEVKARLAEEINLFLNPFREKRSEFV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQPKILEEALQKGTEKMRSVARETMEEVHNNLGLSRKWRSILASSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935