Click here to switch to the map view.

The map label for this gene is gspE

Identifier: 15835474

GI number: 15835474

Start: 993620

End: 995122

Strand: Reverse

Name: gspE

Synonym: TC0860

Alternate gene names: 15835474

Gene position: 995122-993620 (Counterclockwise)

Preceding gene: 15835475

Following gene: 15835473

Centisome position: 92.75

GC content: 43.05

Gene sequence:

>1503_bases
ATGGCCGAGAATAAGTGTTCCATGCAAGATCTTTTAGATCGCCTTCCTTACTCCTTTTTAAAGAAAAATTATCTGCTGCC
TATAGATGACTTAGGGGATAAGATCGTATTGGCGAGGCATCTGAAAAAGACTCCTTTGGAGGCTTTGGATGAAGTGCGGT
TGATTATGCAGAAACCCCTTACCATAGTTTCTAAGGAAGAAACAGAAATTATTCACGGATTGCAAAAGCTTTATAGCGAT
AAGGAGGGGAAGGCCTCTGAAATGCTACTCTCTATGCAAGATGGAGAGGCTCAAGAATCTGAAAGTGACACGACTGAACT
TCTTGAGAGCCAAGAGGATAGTGCGCCTGTTGTTCGATTACTAAATTTGATTCTTAAAGAAGCTATTGAAGAGCGGGCTT
CGGATATTCATTTTGAGCCAGTAGAGGATCTACTGCGTATTCGTTACCGTATTGATGGGGTTCTTCATGATCGTCATGCT
CCTCCCAATCATCTCAGAACGGCTCTCATTACTCGTATTAAGGTGTTGGCTAAACTTGACATAGCCGAACATCGTTTACC
TCAAGATGGGCGCATTAAGTTGCAGTTGGGGGGGCAAGAGATTGATATGCGTGTCAGTACAGTCCCTGTGATTCATGGGG
AACGGGTAGTATTGCGTATATTAGATAAGCGTAATGTGATTTTGGACATACGAGGGTTGTGTATGCTTCCCAAAATGGAA
GCTTCTTTTAGAAAAGCAATTAGTGTCCCTGAAGGTATTTTGTTGGTTACAGGTCCTACAGGAAGCGGGAAGACCACAAC
GTTATATAGTGTTTTACAACATCTGTCCGGGCCTTTTACGAATATTATGACTATTGAAGATCCTCCGGAGTATAAGCTCC
CTGGGATTGCTCAAATTGCTGTAAAACCTAAGATAGGGCTTACCTTTTCCAGAGGGCTTCGTCATTTGTTAAGGCAGGAT
CCGGATGTTCTTATGGTTGGAGAAATTCGTGATCAAGAAACTGCAGAGATTGCAATTCAAGCAGCTTTAACAGGGCATTT
AGTTGTTACTACTCTTCATACTAATGATGCTGTATCCGCGATACCACGTCTTTTAGATATGGGGGTCGAGTCTTATTTAT
TGTCTGCAACCATGATAGGAGCTGTTGCTCAACGACTAGTTCGTAGGATTTGCAAGCATTGTAAAGAAATATGTGAAGCA
GATGTACAAGAACGAGCTTTGCTAAAGGCGTTAGGAAAAGACCCTTATGCTCCTTTGTATAAAGGTCGCGGGTGTTCCCA
ATGTTTTCGTTCAGGATACAAAGGGAGACAGGGGATTTATGAGTTTGTCGATATTACGACGACCCTTCGCTCTGAAATTG
CATTAGGGAAGCCTTATCATGTTTTGCGAGGCGTAGCAGAGCAAGAGGGATATCGGCCTTTGCTTGAGCATGGAGTAGAA
CTAGCTCTTGCAGGAGAGACGACTCTCTCTGAAGTTTTACGTGTCGCTAAACGGTCAGAATAG

Upstream 100 bases:

>100_bases
GTAGTGTCAGGTCAGCAGGCTGCTAAACAGGCTATGGAGAAAAAAGAGTCTATCGCATGGAGAGAAGAAACTCATAGTTT
GCGGGAAGGAGTGGAGTACG

Downstream 100 bases:

>100_bases
CAGGAGGAGGGGATATGGCTAGATTTCTATGCACATATCTTGACCAGAAAGAGAAAAAACGACGCTCTTTTGTAGAGGCT
TTTCATCAACAAGAGGCCAG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAENKCSMQDLLDRLPYSFLKKNYLLPIDDLGDKIVLARHLKKTPLEALDEVRLIMQKPLTIVSKEETEIIHGLQKLYSD
KEGKASEMLLSMQDGEAQESESDTTELLESQEDSAPVVRLLNLILKEAIEERASDIHFEPVEDLLRIRYRIDGVLHDRHA
PPNHLRTALITRIKVLAKLDIAEHRLPQDGRIKLQLGGQEIDMRVSTVPVIHGERVVLRILDKRNVILDIRGLCMLPKME
ASFRKAISVPEGILLVTGPTGSGKTTTLYSVLQHLSGPFTNIMTIEDPPEYKLPGIAQIAVKPKIGLTFSRGLRHLLRQD
PDVLMVGEIRDQETAEIAIQAALTGHLVVTTLHTNDAVSAIPRLLDMGVESYLLSATMIGAVAQRLVRRICKHCKEICEA
DVQERALLKALGKDPYAPLYKGRGCSQCFRSGYKGRQGIYEFVDITTTLRSEIALGKPYHVLRGVAEQEGYRPLLEHGVE
LALAGETTLSEVLRVAKRSE

Sequences:

>Translated_500_residues
MAENKCSMQDLLDRLPYSFLKKNYLLPIDDLGDKIVLARHLKKTPLEALDEVRLIMQKPLTIVSKEETEIIHGLQKLYSD
KEGKASEMLLSMQDGEAQESESDTTELLESQEDSAPVVRLLNLILKEAIEERASDIHFEPVEDLLRIRYRIDGVLHDRHA
PPNHLRTALITRIKVLAKLDIAEHRLPQDGRIKLQLGGQEIDMRVSTVPVIHGERVVLRILDKRNVILDIRGLCMLPKME
ASFRKAISVPEGILLVTGPTGSGKTTTLYSVLQHLSGPFTNIMTIEDPPEYKLPGIAQIAVKPKIGLTFSRGLRHLLRQD
PDVLMVGEIRDQETAEIAIQAALTGHLVVTTLHTNDAVSAIPRLLDMGVESYLLSATMIGAVAQRLVRRICKHCKEICEA
DVQERALLKALGKDPYAPLYKGRGCSQCFRSGYKGRQGIYEFVDITTTLRSEIALGKPYHVLRGVAEQEGYRPLLEHGVE
LALAGETTLSEVLRVAKRSE
>Mature_499_residues
AENKCSMQDLLDRLPYSFLKKNYLLPIDDLGDKIVLARHLKKTPLEALDEVRLIMQKPLTIVSKEETEIIHGLQKLYSDK
EGKASEMLLSMQDGEAQESESDTTELLESQEDSAPVVRLLNLILKEAIEERASDIHFEPVEDLLRIRYRIDGVLHDRHAP
PNHLRTALITRIKVLAKLDIAEHRLPQDGRIKLQLGGQEIDMRVSTVPVIHGERVVLRILDKRNVILDIRGLCMLPKMEA
SFRKAISVPEGILLVTGPTGSGKTTTLYSVLQHLSGPFTNIMTIEDPPEYKLPGIAQIAVKPKIGLTFSRGLRHLLRQDP
DVLMVGEIRDQETAEIAIQAALTGHLVVTTLHTNDAVSAIPRLLDMGVESYLLSATMIGAVAQRLVRRICKHCKEICEAD
VQERALLKALGKDPYAPLYKGRGCSQCFRSGYKGRQGIYEFVDITTTLRSEIALGKPYHVLRGVAEQEGYRPLLEHGVEL
ALAGETTLSEVLRVAKRSE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=467, Percent_Identity=44.1113490364026, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1786296, Length=406, Percent_Identity=44.0886699507389, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=40.8284023668639, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55874; Mature: 55743

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENKCSMQDLLDRLPYSFLKKNYLLPIDDLGDKIVLARHLKKTPLEALDEVRLIMQKPL
CCCCCCCHHHHHHHCCHHHHHCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCC
TIVSKEETEIIHGLQKLYSDKEGKASEMLLSMQDGEAQESESDTTELLESQEDSAPVVRL
CEECCHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCCHHHHHHHCCCCCHHHHHH
LNLILKEAIEERASDIHFEPVEDLLRIRYRIDGVLHDRHAPPNHLRTALITRIKVLAKLD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IAEHRLPQDGRIKLQLGGQEIDMRVSTVPVIHGERVVLRILDKRNVILDIRGLCMLPKME
HHHHCCCCCCEEEEEECCCEEEEEEEEEEEECCCHHHHHHHCCCCEEEEECCCEECCCHH
ASFRKAISVPEGILLVTGPTGSGKTTTLYSVLQHLSGPFTNIMTIEDPPEYKLPGIAQIA
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEE
VKPKIGLTFSRGLRHLLRQDPDVLMVGEIRDQETAEIAIQAALTGHLVVTTLHTNDAVSA
ECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHEECCEEEEEEEECCHHHHH
IPRLLDMGVESYLLSATMIGAVAQRLVRRICKHCKEICEADVQERALLKALGKDPYAPLY
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KGRGCSQCFRSGYKGRQGIYEFVDITTTLRSEIALGKPYHVLRGVAEQEGYRPLLEHGVE
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCE
LALAGETTLSEVLRVAKRSE
EEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AENKCSMQDLLDRLPYSFLKKNYLLPIDDLGDKIVLARHLKKTPLEALDEVRLIMQKPL
CCCCCCHHHHHHHCCHHHHHCCCCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCC
TIVSKEETEIIHGLQKLYSDKEGKASEMLLSMQDGEAQESESDTTELLESQEDSAPVVRL
CEECCHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCCHHHHHHHCCCCCHHHHHH
LNLILKEAIEERASDIHFEPVEDLLRIRYRIDGVLHDRHAPPNHLRTALITRIKVLAKLD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IAEHRLPQDGRIKLQLGGQEIDMRVSTVPVIHGERVVLRILDKRNVILDIRGLCMLPKME
HHHHCCCCCCEEEEEECCCEEEEEEEEEEEECCCHHHHHHHCCCCEEEEECCCEECCCHH
ASFRKAISVPEGILLVTGPTGSGKTTTLYSVLQHLSGPFTNIMTIEDPPEYKLPGIAQIA
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEE
VKPKIGLTFSRGLRHLLRQDPDVLMVGEIRDQETAEIAIQAALTGHLVVTTLHTNDAVSA
ECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHEECCEEEEEEEECCHHHHH
IPRLLDMGVESYLLSATMIGAVAQRLVRRICKHCKEICEADVQERALLKALGKDPYAPLY
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KGRGCSQCFRSGYKGRQGIYEFVDITTTLRSEIALGKPYHVLRGVAEQEGYRPLLEHGVE
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCE
LALAGETTLSEVLRVAKRSE
EEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]