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The map label for this gene is gspF

Identifier: 15835473

GI number: 15835473

Start: 992430

End: 993605

Strand: Reverse

Name: gspF

Synonym: TC0859

Alternate gene names: 15835473

Gene position: 993605-992430 (Counterclockwise)

Preceding gene: 15835474

Following gene: 15835472

Centisome position: 92.6

GC content: 41.75

Gene sequence:

>1176_bases
ATGGCTAGATTTCTATGCACATATCTTGACCAGAAAGAGAAAAAACGACGCTCTTTTGTAGAGGCTTTTCATCAACAAGA
GGCCAGAGAACTCCTTATGTCTCAGGGAGCTCGGATTTTGGATATTCGCAAAGTTCGTGAAAGAAACTATCGAGTCCCTA
CGTCTGAGTTAGTTATTTTTACAAAGCAGTTATCCTTGCTATTACGTTCAGGGGTTTCTTTATACGATTCCCTAGCTTCT
TTGCGAGATCAGTATCAAGGGCGTGCTTTGGCAGGGATTCTTACATCTCTAATGGAGGCTTTACGTTCCGGAGGAGTGTT
TTCAGAGGCTTTGGCTAAATACCCCACAATTTTCGATTCTTTTTATCAAAACAGCGTGCGTTCTGGAGAGAGCGTTGGTA
ATTTAGAAGGGGCCTTAGTGAATATCACTAAGGTATTGGAAGAGAAAGAGAAATTATCCAAAAATATTTCAGCTGCATTA
AGTTATCCAGCAATCTTGTTGGTATTTTCATGTGCGGTGATCGTGTTTTTCTTGGTGGGAGTCATTCCTTCTTTAAAAGA
GAGTTTTGAAGATATAGAGACGACAGGGCTTACAAAAATCGTTTTTTCGTGCAGCGAATGGTTTTGTAAGTATCAGTTTT
TCCTCCTGATAGGGGGAGTTATTGGGGGAGGGGCTTTTCGCACAATTTGGAAGAAGCAGATCGGAAGAAAGACTATAGAA
GGGTTAGTAAAAAACATCCCTATCTTACGCAACTTCATCATTAAAATAGGCTTTTGTCGCTTCTGCTCAGTAGCTTCTGC
CGTTTTGCAGGGGGGAGGAAATCTAATTGAGGCTCTTAAGTTGGGTTGTGGGGCTATTCCTCAAGATTTTTTACGAGAAG
AATTGCAGACAGTGATTCAAGCAGTCATTCGAGGAGGGTCTTTGAGTCGGGAGTTATCGCGCTATTCTTGGACCCCCAAA
TTGGTTACAGGGATGGTTGCCCTAGGAGAGGAATCTGGGGATCTTTCCGTGGTATTCGCGCATATTGCGCAAATTTATAA
CGAAGATATTCAAAGGGTTTTAGCTTGGTTAACGGCCTGGTGTCAGCCCATTGTTCTGGTGTTGTTAGGAGGGTTTATTG
GATTGATTATGTTATCCATTCTTCTTCCGTTAACAAGTGGTATTCAAACGTTTTAA

Upstream 100 bases:

>100_bases
CTTTGCTTGAGCATGGAGTAGAACTAGCTCTTGCAGGAGAGACGACTCTCTCTGAAGTTTTACGTGTCGCTAAACGGTCA
GAATAGCAGGAGGAGGGGAT

Downstream 100 bases:

>100_bases
CTATAGGGGGAAACTGTGGGGAAAACAAAAAAAAGAAAGCAATCCATCACGTTAATTGAAATGATGGTGGTTATTACTCT
CATTGGCATCATCAGTGGGG

Product: general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MARFLCTYLDQKEKKRRSFVEAFHQQEARELLMSQGARILDIRKVRERNYRVPTSELVIFTKQLSLLLRSGVSLYDSLAS
LRDQYQGRALAGILTSLMEALRSGGVFSEALAKYPTIFDSFYQNSVRSGESVGNLEGALVNITKVLEEKEKLSKNISAAL
SYPAILLVFSCAVIVFFLVGVIPSLKESFEDIETTGLTKIVFSCSEWFCKYQFFLLIGGVIGGGAFRTIWKKQIGRKTIE
GLVKNIPILRNFIIKIGFCRFCSVASAVLQGGGNLIEALKLGCGAIPQDFLREELQTVIQAVIRGGSLSRELSRYSWTPK
LVTGMVALGEESGDLSVVFAHIAQIYNEDIQRVLAWLTAWCQPIVLVLLGGFIGLIMLSILLPLTSGIQTF

Sequences:

>Translated_391_residues
MARFLCTYLDQKEKKRRSFVEAFHQQEARELLMSQGARILDIRKVRERNYRVPTSELVIFTKQLSLLLRSGVSLYDSLAS
LRDQYQGRALAGILTSLMEALRSGGVFSEALAKYPTIFDSFYQNSVRSGESVGNLEGALVNITKVLEEKEKLSKNISAAL
SYPAILLVFSCAVIVFFLVGVIPSLKESFEDIETTGLTKIVFSCSEWFCKYQFFLLIGGVIGGGAFRTIWKKQIGRKTIE
GLVKNIPILRNFIIKIGFCRFCSVASAVLQGGGNLIEALKLGCGAIPQDFLREELQTVIQAVIRGGSLSRELSRYSWTPK
LVTGMVALGEESGDLSVVFAHIAQIYNEDIQRVLAWLTAWCQPIVLVLLGGFIGLIMLSILLPLTSGIQTF
>Mature_390_residues
ARFLCTYLDQKEKKRRSFVEAFHQQEARELLMSQGARILDIRKVRERNYRVPTSELVIFTKQLSLLLRSGVSLYDSLASL
RDQYQGRALAGILTSLMEALRSGGVFSEALAKYPTIFDSFYQNSVRSGESVGNLEGALVNITKVLEEKEKLSKNISAALS
YPAILLVFSCAVIVFFLVGVIPSLKESFEDIETTGLTKIVFSCSEWFCKYQFFLLIGGVIGGGAFRTIWKKQIGRKTIEG
LVKNIPILRNFIIKIGFCRFCSVASAVLQGGGNLIEALKLGCGAIPQDFLREELQTVIQAVIRGGSLSRELSRYSWTPKL
VTGMVALGEESGDLSVVFAHIAQIYNEDIQRVLAWLTAWCQPIVLVLLGGFIGLIMLSILLPLTSGIQTF

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=383, Percent_Identity=25.5874673629243, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786295, Length=366, Percent_Identity=22.4043715846995, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43392; Mature: 43260

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFLCTYLDQKEKKRRSFVEAFHQQEARELLMSQGARILDIRKVRERNYRVPTSELVIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHH
TKQLSLLLRSGVSLYDSLASLRDQYQGRALAGILTSLMEALRSGGVFSEALAKYPTIFDS
HHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FYQNSVRSGESVGNLEGALVNITKVLEEKEKLSKNISAALSYPAILLVFSCAVIVFFLVG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIPSLKESFEDIETTGLTKIVFSCSEWFCKYQFFLLIGGVIGGGAFRTIWKKQIGRKTIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GLVKNIPILRNFIIKIGFCRFCSVASAVLQGGGNLIEALKLGCGAIPQDFLREELQTVIQ
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AVIRGGSLSRELSRYSWTPKLVTGMVALGEESGDLSVVFAHIAQIYNEDIQRVLAWLTAW
HHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CQPIVLVLLGGFIGLIMLSILLPLTSGIQTF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARFLCTYLDQKEKKRRSFVEAFHQQEARELLMSQGARILDIRKVRERNYRVPTSELVIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHH
TKQLSLLLRSGVSLYDSLASLRDQYQGRALAGILTSLMEALRSGGVFSEALAKYPTIFDS
HHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FYQNSVRSGESVGNLEGALVNITKVLEEKEKLSKNISAALSYPAILLVFSCAVIVFFLVG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIPSLKESFEDIETTGLTKIVFSCSEWFCKYQFFLLIGGVIGGGAFRTIWKKQIGRKTIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GLVKNIPILRNFIIKIGFCRFCSVASAVLQGGGNLIEALKLGCGAIPQDFLREELQTVIQ
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AVIRGGSLSRELSRYSWTPKLVTGMVALGEESGDLSVVFAHIAQIYNEDIQRVLAWLTAW
HHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CQPIVLVLLGGFIGLIMLSILLPLTSGIQTF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]