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Identifier: 15835460

GI number: 15835460

Start: 982146

End: 983543

Strand: Direct

Name: Not Available

Synonym: TC0846

Alternate gene names: 15835460

Gene position: 982146-983543 (Clockwise)

Preceding gene: 15835459

Following gene: 15835461

Centisome position: 91.54

GC content: 44.35

Gene sequence:

>1398_bases
ATGAATGAAGCTTTCGACTGTGTAGTGATTGGAGCAGGGCCTGGAGGCTATGTTGCCGCCATTACTGCCGCCCAAGCAGG
ATTAAAAACTGCGCTAATCGAAGAGCGGGAAGCTGGAGGAACCTGTTTAAACCGGGGATGCATTCCTTCTAAAGCTCTAC
TTGCATCGGCAGAAATTGTTGCCCAAATACGCCATGCTGACCAATTTGGCATTCATATTAATGGTTTCAGCATTGACTAT
CCCGCTATGGTACAAAGAAAGGATACTGTTGTTCGCAGTATTCGTGATGGGCTTAATGGCTTGATTCGTAGCAACAAAAT
CACTGTGTTTTCTGGAAGAGGTTCCTTAATTTCTTCAACAGAAGTAAAAATTTTGGGGGAAACTCCTTCTGTAATCAAAG
CACAATCCATTATCCTGGCTACAGGATCTGAACCTAGAGCCTTTCCTGGGGTCCCTTTTTCCCAACAATCTCCTAGAATC
TTATGCTCGACAGGAGTTCTTAACCTAAAAGAAATCCCTCAAAAAATGGCTATTATCGGAGGTGGGGTCATTGGATGTGA
ATTCGCTTCCTTATTCCATACCCTAGGATCAGAAGTTTCCGTCATCGAAGCAAGCCAACAAATTCTAGCCCTGAATAATC
CAGATATTTCGAAAACCATGTTCGATAAGTTCACGCGTCATGGCATTCGCTTTATGTTAGGAGCGTCTGTGTCGAGCATT
GAGGATATGGGTGATCGCGTTCGACTAACTATTAACGGAAATATTGAAGAATACGATTACGTTCTCGTATCCATAGGACG
TCGATTGAATACAGAAAATATTGGATTAGATAAAGCCGGTGTGATTTGTGACGAACGCGGAGTCATCCCTACAGATTCCA
CTATGCGCACTAACGTTCCTAATATTTATGCTATTGGAGATATCACAGGGAAATGGCAACTTGCCCATGTGGCCTCCCAC
CAGGGAATTGTTGCTGCTCGAAATATAGCTGGACATAAGGATGAAATCGATTATTCTGCAGTCCCTTCCGTAATTTTTAC
TTTCCCGGAAGTTGCATCCGTGGGACTTTCTCCGACATCAGCTCAACAACAAGGAATTCCTGTCAAAGTAACGAAATTCC
CATTCCGAGCCATTGGGAAAGCCGTTGCTATGGGCGAATCCGACGGATTTGCTGCCATTATCAGCCATGAAACTTCTCAG
CAAATTCTAGGTGCTTACGTTATTGGCCCTCACGCCTCTTCTCTTATTTCCGAAATCACCTTAGCTATTCGCAATGAGCT
GACCCTCCCCTGCATTTATGAAACCATCCATGCACATCCAACCTTAGCAGAAGTTTGGGCAGAAAGTGCACTATTGGCCG
TTGATACCCCGTTACATATGCCCCCTACTAGAAAATGA

Upstream 100 bases:

>100_bases
CCCGTTCTAAAAGCATTAAGGGAGAGAAACCTTTCCTCTTAAGGCTAATCATCTCATGTGGATCGTTGCAATCCTAAACA
CTCTTTAAAAAGGAAACACT

Downstream 100 bases:

>100_bases
GTAATTCCCCAGAATCCTCAACTCCCAAGCAGTCCATCCCAGCACGATTCCCCAAATGGTTGCGACAAAAACTTCCTCTA
GGGAAAGTATTTTCTCGAAC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNEAFDCVVIGAGPGGYVAAITAAQAGLKTALIEEREAGGTCLNRGCIPSKALLASAEIVAQIRHADQFGIHINGFSIDY
PAMVQRKDTVVRSIRDGLNGLIRSNKITVFSGRGSLISSTEVKILGETPSVIKAQSIILATGSEPRAFPGVPFSQQSPRI
LCSTGVLNLKEIPQKMAIIGGGVIGCEFASLFHTLGSEVSVIEASQQILALNNPDISKTMFDKFTRHGIRFMLGASVSSI
EDMGDRVRLTINGNIEEYDYVLVSIGRRLNTENIGLDKAGVICDERGVIPTDSTMRTNVPNIYAIGDITGKWQLAHVASH
QGIVAARNIAGHKDEIDYSAVPSVIFTFPEVASVGLSPTSAQQQGIPVKVTKFPFRAIGKAVAMGESDGFAAIISHETSQ
QILGAYVIGPHASSLISEITLAIRNELTLPCIYETIHAHPTLAEVWAESALLAVDTPLHMPPTRK

Sequences:

>Translated_465_residues
MNEAFDCVVIGAGPGGYVAAITAAQAGLKTALIEEREAGGTCLNRGCIPSKALLASAEIVAQIRHADQFGIHINGFSIDY
PAMVQRKDTVVRSIRDGLNGLIRSNKITVFSGRGSLISSTEVKILGETPSVIKAQSIILATGSEPRAFPGVPFSQQSPRI
LCSTGVLNLKEIPQKMAIIGGGVIGCEFASLFHTLGSEVSVIEASQQILALNNPDISKTMFDKFTRHGIRFMLGASVSSI
EDMGDRVRLTINGNIEEYDYVLVSIGRRLNTENIGLDKAGVICDERGVIPTDSTMRTNVPNIYAIGDITGKWQLAHVASH
QGIVAARNIAGHKDEIDYSAVPSVIFTFPEVASVGLSPTSAQQQGIPVKVTKFPFRAIGKAVAMGESDGFAAIISHETSQ
QILGAYVIGPHASSLISEITLAIRNELTLPCIYETIHAHPTLAEVWAESALLAVDTPLHMPPTRK
>Mature_465_residues
MNEAFDCVVIGAGPGGYVAAITAAQAGLKTALIEEREAGGTCLNRGCIPSKALLASAEIVAQIRHADQFGIHINGFSIDY
PAMVQRKDTVVRSIRDGLNGLIRSNKITVFSGRGSLISSTEVKILGETPSVIKAQSIILATGSEPRAFPGVPFSQQSPRI
LCSTGVLNLKEIPQKMAIIGGGVIGCEFASLFHTLGSEVSVIEASQQILALNNPDISKTMFDKFTRHGIRFMLGASVSSI
EDMGDRVRLTINGNIEEYDYVLVSIGRRLNTENIGLDKAGVICDERGVIPTDSTMRTNVPNIYAIGDITGKWQLAHVASH
QGIVAARNIAGHKDEIDYSAVPSVIFTFPEVASVGLSPTSAQQQGIPVKVTKFPFRAIGKAVAMGESDGFAAIISHETSQ
QILGAYVIGPHASSLISEITLAIRNELTLPCIYETIHAHPTLAEVWAESALLAVDTPLHMPPTRK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=39.3088552915767, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=26.7818574514039, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI148277071, Length=436, Percent_Identity=28.2110091743119, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI148277065, Length=429, Percent_Identity=27.972027972028, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI33519430, Length=429, Percent_Identity=27.972027972028, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI33519428, Length=429, Percent_Identity=27.972027972028, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI33519426, Length=429, Percent_Identity=27.972027972028, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI22035672, Length=452, Percent_Identity=26.9911504424779, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI291045266, Length=431, Percent_Identity=26.2180974477958, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI291045268, Length=428, Percent_Identity=24.2990654205607, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1786307, Length=444, Percent_Identity=38.963963963964, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=29.3333333333333, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=28.0701754385965, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=28.4722222222222, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1789065, Length=259, Percent_Identity=29.7297297297297, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=459, Percent_Identity=37.2549019607843, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=28.5412262156448, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71983429, Length=456, Percent_Identity=26.0964912280702, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=456, Percent_Identity=26.0964912280702, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=434, Percent_Identity=23.0414746543779, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=481, Percent_Identity=36.3825363825364, Blast_Score=286, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=29.6774193548387, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=27.9411764705882, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=40.4761904761905, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24640553, Length=498, Percent_Identity=28.714859437751, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24640549, Length=498, Percent_Identity=28.714859437751, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24640551, Length=498, Percent_Identity=28.714859437751, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=26.1052631578947, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH_CHLMU (Q9PJI3)

Other databases:

- EMBL:   AE002160
- PIR:   B81658
- RefSeq:   NP_297219.1
- ProteinModelPortal:   Q9PJI3
- SMR:   Q9PJI3
- GeneID:   1246214
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0846
- TIGR:   TC_0846
- HOGENOM:   HBG515043
- OMA:   NTTDIGL
- PhylomeDB:   Q9PJI3
- ProtClustDB:   PRK06327
- BioCyc:   CMUR243161:TC_0846-MONOMER
- BRENDA:   1.8.1.4
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 49563; Mature: 49563

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 439-439 BINDING 51-51 BINDING 114-114 BINDING 203-203 BINDING 237-237 BINDING 307-307 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAFDCVVIGAGPGGYVAAITAAQAGLKTALIEEREAGGTCLNRGCIPSKALLASAEIV
CCCCEEEEEEECCCCCEEEEEHHHHHCHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHH
AQIRHADQFGIHINGFSIDYPAMVQRKDTVVRSIRDGLNGLIRSNKITVFSGRGSLISST
HHHHCCCCCEEEEECEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCC
EVKILGETPSVIKAQSIILATGSEPRAFPGVPFSQQSPRILCSTGVLNLKEIPQKMAIIG
EEEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHC
GGVIGCEFASLFHTLGSEVSVIEASQQILALNNPDISKTMFDKFTRHGIRFMLGASVSSI
CCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCHHHH
EDMGDRVRLTINGNIEEYDYVLVSIGRRLNTENIGLDKAGVICDERGVIPTDSTMRTNVP
HCCCCEEEEEEECCCCHHHEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
NIYAIGDITGKWQLAHVASHQGIVAARNIAGHKDEIDYSAVPSVIFTFPEVASVGLSPTS
CEEEEECCCCCEEEEEECCCCCEEEECCCCCCCCCCCHHHHCHHHHCCHHHHHCCCCCCC
AQQQGIPVKVTKFPFRAIGKAVAMGESDGFAAIISHETSQQILGAYVIGPHASSLISEIT
HHHCCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHEEEEECCCHHHHHHHHH
LAIRNELTLPCIYETIHAHPTLAEVWAESALLAVDTPLHMPPTRK
HHHHCCCCCHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MNEAFDCVVIGAGPGGYVAAITAAQAGLKTALIEEREAGGTCLNRGCIPSKALLASAEIV
CCCCEEEEEEECCCCCEEEEEHHHHHCHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHH
AQIRHADQFGIHINGFSIDYPAMVQRKDTVVRSIRDGLNGLIRSNKITVFSGRGSLISST
HHHHCCCCCEEEEECEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCC
EVKILGETPSVIKAQSIILATGSEPRAFPGVPFSQQSPRILCSTGVLNLKEIPQKMAIIG
EEEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHC
GGVIGCEFASLFHTLGSEVSVIEASQQILALNNPDISKTMFDKFTRHGIRFMLGASVSSI
CCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCHHHH
EDMGDRVRLTINGNIEEYDYVLVSIGRRLNTENIGLDKAGVICDERGVIPTDSTMRTNVP
HCCCCEEEEEEECCCCHHHEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
NIYAIGDITGKWQLAHVASHQGIVAARNIAGHKDEIDYSAVPSVIFTFPEVASVGLSPTS
CEEEEECCCCCEEEEEECCCCCEEEECCCCCCCCCCCHHHHCHHHHCCHHHHHCCCCCCC
AQQQGIPVKVTKFPFRAIGKAVAMGESDGFAAIISHETSQQILGAYVIGPHASSLISEIT
HHHCCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHEEEEECCCHHHHHHHHH
LAIRNELTLPCIYETIHAHPTLAEVWAESALLAVDTPLHMPPTRK
HHHHCCCCCHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935