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The map label for this gene is hisS

Identifier: 15835444

GI number: 15835444

Start: 961678

End: 962964

Strand: Reverse

Name: hisS

Synonym: TC0830

Alternate gene names: 15835444

Gene position: 962964-961678 (Counterclockwise)

Preceding gene: 15835448

Following gene: 15835443

Centisome position: 89.75

GC content: 42.11

Gene sequence:

>1287_bases
ATGAGTAACGTATTGCCGAAAGGCGTTTTCGATATTTTCCCCTATGTAACAAATTCAAAGAATCTTTGGAGAAATTCTTT
TCTATGGAAAACGGTTGAACATGCTGCTCACCGTATATGTAATTTGTATGGGTTTGATGAGATTCGCACGCCAGTTTTTG
AAAAGACGGAGACTTTCTTACGTGTTGGTGAGCACAGCGACATTGTAAAAAAGGAAGTTTATACTTTTTTGGATAAAAAG
GGGCGCTCTTTAACCTTGCGTCCAGAAGGAACTGCAGCCGTTGTTCGAGCTTTGCTGGATCATTCTGCTGATATGCGTAA
AGACAATAAAATTTATTATATTTTGCCAATGTTTCGTTATGAACGGCAGCAATCTGGGCGTTACCGGCAGCACCATCAGT
TTGGGTTAGAGGCTATTGGTGTGCGGCATCCTTTGCGAGATGCTGAAGTTTTGTCTTTACTGTGGGATTTTTATGCGGCA
GTTGGGCTTAAGCATATGCAGATCCAAGTAAATTTTTTAGGGGGCCGGAAGACCCGGGCTCGTTATGATGAGGCATTGCG
AGAGTTTTTCCGTAAAGATTTAGACAAATTATCTACGCTAAGCCAAGAACGGTTCCATGCGAATTTGTTGCGCATTTTAG
ATTCTAAAGAGCCAGAAGACCAAGCCTTTATTGAACAGGCTCCTTCTATTCTAGATTACGTTGATGATCAAGATTTGAAT
TATTTTGATTCGGTGTTAGCTGAGTTAAAGGTTTTGGGGATCCCTTATAAAATTAATCCACGGTTGGTTCGCGGATTAGA
TTATTACACCGATCTAGTGTTTGAAGCTGTAACTGTTGTTGGTGACCATTCCTATGCGCTAGGAGGTGGGGGACGTTATG
ATGAGTTAGTGGCACAATCCGGTGGCCCCGCAATGCCTGCTTTTGGTTTCGGAGTGGGGTTAGAAAGGGTAATTCAAACT
TTATTAGAACAAGGAGCTGCTTTGCCAACAGCTCCACGTCGATTGCGATTGATTCCTATGGATGAGAGTGCGGATGCTTT
TTGTTTTTCTTGGGCGAAGCATTTGCGTCATTTAGGTGTTGCAACGGAAGTAGATTGGGCTCATAAGAAACCAAAAACAG
CACTTAAAGATGCTGCTGATCAACAGGTGGGCTTTGTTTGCTTTGTAGGCGAGCAAGAGCTGTCTACGAAACAGTTTATA
GTTAAAGATATGTCTCTACATCAAAGCTTTTCAGGGGCTCAACAAGATGTAGAACAAAGGTTGGTTTATGAAGTACAGAA
CGCATAA

Upstream 100 bases:

>100_bases
TGGTGTAACATGATGGGCTCGTTCTTTATGCAAAAAAGAACTTTAAGAAAGTAAAATTAGATTGCAAGTCTCTTAGCGGA
GTATAATCCGGTTATACAGA

Downstream 100 bases:

>100_bases
ATGTAATGAGTTGTCCCTTAATCATGTTGGGGAACATGTTCGTTTGTCTGGGTGGGTGCATCGTTACCGTAACCATGGTG
GAGTTGTTTTTATTGATTTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSNVLPKGVFDIFPYVTNSKNLWRNSFLWKTVEHAAHRICNLYGFDEIRTPVFEKTETFLRVGEHSDIVKKEVYTFLDKK
GRSLTLRPEGTAAVVRALLDHSADMRKDNKIYYILPMFRYERQQSGRYRQHHQFGLEAIGVRHPLRDAEVLSLLWDFYAA
VGLKHMQIQVNFLGGRKTRARYDEALREFFRKDLDKLSTLSQERFHANLLRILDSKEPEDQAFIEQAPSILDYVDDQDLN
YFDSVLAELKVLGIPYKINPRLVRGLDYYTDLVFEAVTVVGDHSYALGGGGRYDELVAQSGGPAMPAFGFGVGLERVIQT
LLEQGAALPTAPRRLRLIPMDESADAFCFSWAKHLRHLGVATEVDWAHKKPKTALKDAADQQVGFVCFVGEQELSTKQFI
VKDMSLHQSFSGAQQDVEQRLVYEVQNA

Sequences:

>Translated_428_residues
MSNVLPKGVFDIFPYVTNSKNLWRNSFLWKTVEHAAHRICNLYGFDEIRTPVFEKTETFLRVGEHSDIVKKEVYTFLDKK
GRSLTLRPEGTAAVVRALLDHSADMRKDNKIYYILPMFRYERQQSGRYRQHHQFGLEAIGVRHPLRDAEVLSLLWDFYAA
VGLKHMQIQVNFLGGRKTRARYDEALREFFRKDLDKLSTLSQERFHANLLRILDSKEPEDQAFIEQAPSILDYVDDQDLN
YFDSVLAELKVLGIPYKINPRLVRGLDYYTDLVFEAVTVVGDHSYALGGGGRYDELVAQSGGPAMPAFGFGVGLERVIQT
LLEQGAALPTAPRRLRLIPMDESADAFCFSWAKHLRHLGVATEVDWAHKKPKTALKDAADQQVGFVCFVGEQELSTKQFI
VKDMSLHQSFSGAQQDVEQRLVYEVQNA
>Mature_427_residues
SNVLPKGVFDIFPYVTNSKNLWRNSFLWKTVEHAAHRICNLYGFDEIRTPVFEKTETFLRVGEHSDIVKKEVYTFLDKKG
RSLTLRPEGTAAVVRALLDHSADMRKDNKIYYILPMFRYERQQSGRYRQHHQFGLEAIGVRHPLRDAEVLSLLWDFYAAV
GLKHMQIQVNFLGGRKTRARYDEALREFFRKDLDKLSTLSQERFHANLLRILDSKEPEDQAFIEQAPSILDYVDDQDLNY
FDSVLAELKVLGIPYKINPRLVRGLDYYTDLVFEAVTVVGDHSYALGGGGRYDELVAQSGGPAMPAFGFGVGLERVIQTL
LEQGAALPTAPRRLRLIPMDESADAFCFSWAKHLRHLGVATEVDWAHKKPKTALKDAADQQVGFVCFVGEQELSTKQFIV
KDMSLHQSFSGAQQDVEQRLVYEVQNA

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=405, Percent_Identity=37.7777777777778, Blast_Score=262, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_CHLMU (Q9PJJ9)

Other databases:

- EMBL:   AE002160
- PIR:   F81660
- RefSeq:   NP_297203.1
- ProteinModelPortal:   Q9PJJ9
- SMR:   Q9PJJ9
- GeneID:   1246198
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0830
- TIGR:   TC_0830
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q9PJJ9
- ProtClustDB:   PRK00037
- BioCyc:   CMUR243161:TC_0830-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48940; Mature: 48809

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVLPKGVFDIFPYVTNSKNLWRNSFLWKTVEHAAHRICNLYGFDEIRTPVFEKTETFL
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHH
RVGEHSDIVKKEVYTFLDKKGRSLTLRPEGTAAVVRALLDHSADMRKDNKIYYILPMFRY
HCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECHHHH
ERQQSGRYRQHHQFGLEAIGVRHPLRDAEVLSLLWDFYAAVGLKHMQIQVNFLGGRKTRA
HHHHCCCHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHH
RYDEALREFFRKDLDKLSTLSQERFHANLLRILDSKEPEDQAFIEQAPSILDYVDDQDLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCCCCCC
YFDSVLAELKVLGIPYKINPRLVRGLDYYTDLVFEAVTVVGDHSYALGGGGRYDELVAQS
HHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCC
GGPAMPAFGFGVGLERVIQTLLEQGAALPTAPRRLRLIPMDESADAFCFSWAKHLRHLGV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
ATEVDWAHKKPKTALKDAADQQVGFVCFVGEQELSTKQFIVKDMSLHQSFSGAQQDVEQR
CCCCCHHHCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LVYEVQNA
HHHHHCCC
>Mature Secondary Structure 
SNVLPKGVFDIFPYVTNSKNLWRNSFLWKTVEHAAHRICNLYGFDEIRTPVFEKTETFL
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHH
RVGEHSDIVKKEVYTFLDKKGRSLTLRPEGTAAVVRALLDHSADMRKDNKIYYILPMFRY
HCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECHHHH
ERQQSGRYRQHHQFGLEAIGVRHPLRDAEVLSLLWDFYAAVGLKHMQIQVNFLGGRKTRA
HHHHCCCHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHH
RYDEALREFFRKDLDKLSTLSQERFHANLLRILDSKEPEDQAFIEQAPSILDYVDDQDLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCCCCCC
YFDSVLAELKVLGIPYKINPRLVRGLDYYTDLVFEAVTVVGDHSYALGGGGRYDELVAQS
HHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCC
GGPAMPAFGFGVGLERVIQTLLEQGAALPTAPRRLRLIPMDESADAFCFSWAKHLRHLGV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
ATEVDWAHKKPKTALKDAADQQVGFVCFVGEQELSTKQFIVKDMSLHQSFSGAQQDVEQR
CCCCCHHHCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LVYEVQNA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935