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Identifier: 15835441

GI number: 15835441

Start: 958613

End: 959068

Strand: Reverse

Name: Not Available

Synonym: TC0827

Alternate gene names: 15835441

Gene position: 959068-958613 (Counterclockwise)

Preceding gene: 15835442

Following gene: 15835439

Centisome position: 89.39

GC content: 39.91

Gene sequence:

>456_bases
ATGCGCATAGTTCTTCATAATCCGGATATTCCTCAAAATACAGGGAATATTGGTAGAACGTGTGTAGCTTTGGGGGCCGA
GCTAATATTAGTTCGGCCCTTGGGTTTTTCTTTAGCGGATAAGTTTGTAAAGAGAGCTGGGATGGATTATTGGGATCGGC
TTTCTTTGTCTGTAGTTGATTCTTTAGAAGAGGCTCTTAGGGGAATCCCCAGAGAAAAAATTTTCTGTTTAACTACAAAA
GGCTCTTCCTACTATGGAGAACGAGAGCTTCCACTAGATGGCACTTACATTTTTGGGGCAGAATCAAGAGGTTTACCACA
AACAGTTCTGGATGCCTATTCATCTCAGTGCTTGTATATTCCTATGATTGAAGGGACGCGATCTTTAAATTTAGCTACTT
CTGTAGGGATTGTTATGTATGAAGTGGCTCGACAAAATTATCAGAATTTATTTTGA

Upstream 100 bases:

>100_bases
AGGACAATTGCCTCCAAATTCCTTACTCATTTTTGAAGTGAAGTTAATTGAGGCAAATGACGATAATGTATCCGTTGCGG
AGTAATTATTAGGATTTTTT

Downstream 100 bases:

>100_bases
AGTATCTTAAGGCCGAGAAACTCTCGGCCTTTTTTATGAATCACTTTATTGATGTTTAGAAATAAGCTTGACTAGAGAAT
CTTTATCTTTCAAGCCGACG

Product: spoU rRNA methylase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MRIVLHNPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDRLSLSVVDSLEEALRGIPREKIFCLTTK
GSSYYGERELPLDGTYIFGAESRGLPQTVLDAYSSQCLYIPMIEGTRSLNLATSVGIVMYEVARQNYQNLF

Sequences:

>Translated_151_residues
MRIVLHNPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDRLSLSVVDSLEEALRGIPREKIFCLTTK
GSSYYGERELPLDGTYIFGAESRGLPQTVLDAYSSQCLYIPMIEGTRSLNLATSVGIVMYEVARQNYQNLF
>Mature_151_residues
MRIVLHNPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDRLSLSVVDSLEEALRGIPREKIFCLTTK
GSSYYGERELPLDGTYIFGAESRGLPQTVLDAYSSQCLYIPMIEGTRSLNLATSVGIVMYEVARQNYQNLF

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=147, Percent_Identity=40.1360544217687, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16842; Mature: 16842

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVLHNPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDRLSLSVVD
CEEEEECCCCCCCCCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHH
SLEEALRGIPREKIFCLTTKGSSYYGERELPLDGTYIFGAESRGLPQTVLDAYSSQCLYI
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCEEEE
PMIEGTRSLNLATSVGIVMYEVARQNYQNLF
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIVLHNPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDRLSLSVVD
CEEEEECCCCCCCCCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHH
SLEEALRGIPREKIFCLTTKGSSYYGERELPLDGTYIFGAESRGLPQTVLDAYSSQCLYI
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCEEEE
PMIEGTRSLNLATSVGIVMYEVARQNYQNLF
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]