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The map label for this gene is fmt

Identifier: 15835431

GI number: 15835431

Start: 950094

End: 951044

Strand: Reverse

Name: fmt

Synonym: TC0817

Alternate gene names: 15835431

Gene position: 951044-950094 (Counterclockwise)

Preceding gene: 15835432

Following gene: 15835430

Centisome position: 88.64

GC content: 44.9

Gene sequence:

>951_bases
TTGTTGAATCTTAGAGTCGTTTATCTGGGGACCCCCCAGTTTGCGGCTACTGTTTTAGAAACACTGGTAGATGCACGTAT
TCATGTTGTTGGTGTCGTTACACGGGCTGATAAACCACAGAAACGCTCGTCTAAGCCAATTGCTTCTCCAGTAAAACAGT
TAGCCTTATCCAAAAATATTCCTTTACTTCAGCCCACCAAGACTACCGATCCTGCTTTTCTTGCTCAATTGCGAGAATGG
CAGGCAGATGTATTTGTTGTTGTCGCGTACGGAGTGATTTTAAAACAGGAACTTCTAGATATCCCTAAATATGGTTGTTA
CAATCTTCATGCAGGGTTATTGCCCGCGTATCGAGGAGCTGCACCCATTCAGCGTTGTATTATAGCTGGAGAAACTCTTT
CTGGGAACACTGTGATCCGTATGGATGCAGGAATGGATACTGGAGATATTGCCAATGTCAATCATGTAGCTATTGGTGAA
GATATGACTGCAGGAGAATTGGCCGAAGCTCTTGCAGGTTCCGGAGGAGAGCTTATATTAAAAACTTTACAAGAAATTGA
GGCAGGAACAGTCCGCCACATTCCTCAAGATAGCGCAAAAGCCACCCTAGCTCCTAAGTTAACTAAGGAAGAGGGCTTGG
TGAAATGGGATGCACCAGCTTCTCAGGTTTATGCTCATATTCGAGGGGTTTCTCCTGCTCCAGGAGCTTGGACTCGTTTT
CTTTCTCAAGGGAAGGAGCCTCGACGTCTTGGGATACTCTCCGCTAGAATGGAATCCTCTTCTGGTTCCCATTCTCCAGG
AGAAGTGCTTGGTGTTTCCGGGGAAGATTTACTGGTTGCCTGCCGTCAGGGAGTTTTGCGCTTATGTATAGTACAACCTG
AAGGAAAAGTTTTCATGAAAGCAAAAGATTTTTTTAATGGGCAATCTCGATCAGTCGCTTTGCTTTTCTAA

Upstream 100 bases:

>100_bases
TTCGTTGAATTTTGTCGGCAGCCAAGCAAACGTGGTATAGAGAGAGGCATCGATTGCGAAGCATCTTTGGATGAGCCGAT
AGATAAAAAAGAAGGAGCTT

Downstream 100 bases:

>100_bases
AAAAATTTTGTCAGAAAGGTTGTGCAGAACTTTCTTTTTTCGATTTAAGTAATAGAAATGTTACAATGGCTCCCTAAATT
TTATGTATACATAACTTTGA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLNLRVVYLGTPQFAATVLETLVDARIHVVGVVTRADKPQKRSSKPIASPVKQLALSKNIPLLQPTKTTDPAFLAQLREW
QADVFVVVAYGVILKQELLDIPKYGCYNLHAGLLPAYRGAAPIQRCIIAGETLSGNTVIRMDAGMDTGDIANVNHVAIGE
DMTAGELAEALAGSGGELILKTLQEIEAGTVRHIPQDSAKATLAPKLTKEEGLVKWDAPASQVYAHIRGVSPAPGAWTRF
LSQGKEPRRLGILSARMESSSGSHSPGEVLGVSGEDLLVACRQGVLRLCIVQPEGKVFMKAKDFFNGQSRSVALLF

Sequences:

>Translated_316_residues
MLNLRVVYLGTPQFAATVLETLVDARIHVVGVVTRADKPQKRSSKPIASPVKQLALSKNIPLLQPTKTTDPAFLAQLREW
QADVFVVVAYGVILKQELLDIPKYGCYNLHAGLLPAYRGAAPIQRCIIAGETLSGNTVIRMDAGMDTGDIANVNHVAIGE
DMTAGELAEALAGSGGELILKTLQEIEAGTVRHIPQDSAKATLAPKLTKEEGLVKWDAPASQVYAHIRGVSPAPGAWTRF
LSQGKEPRRLGILSARMESSSGSHSPGEVLGVSGEDLLVACRQGVLRLCIVQPEGKVFMKAKDFFNGQSRSVALLF
>Mature_316_residues
MLNLRVVYLGTPQFAATVLETLVDARIHVVGVVTRADKPQKRSSKPIASPVKQLALSKNIPLLQPTKTTDPAFLAQLREW
QADVFVVVAYGVILKQELLDIPKYGCYNLHAGLLPAYRGAAPIQRCIIAGETLSGNTVIRMDAGMDTGDIANVNHVAIGE
DMTAGELAEALAGSGGELILKTLQEIEAGTVRHIPQDSAKATLAPKLTKEEGLVKWDAPASQVYAHIRGVSPAPGAWTRF
LSQGKEPRRLGILSARMESSSGSHSPGEVLGVSGEDLLVACRQGVLRLCIVQPEGKVFMKAKDFFNGQSRSVALLF

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=326, Percent_Identity=25.4601226993865, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI164663775, Length=332, Percent_Identity=26.5060240963855, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI238814322, Length=241, Percent_Identity=27.8008298755187, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789683, Length=306, Percent_Identity=37.9084967320261, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788589, Length=298, Percent_Identity=27.8523489932886, Blast_Score=125, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45550868, Length=318, Percent_Identity=28.3018867924528, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571984, Length=293, Percent_Identity=29.0102389078498, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585660, Length=276, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_CHLMU (Q9PJL2)

Other databases:

- EMBL:   AE002160
- PIR:   F81661
- RefSeq:   NP_297190.1
- ProteinModelPortal:   Q9PJL2
- SMR:   Q9PJL2
- GeneID:   1246184
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0817
- TIGR:   TC_0817
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q9PJL2
- ProtClustDB:   PRK00005
- BioCyc:   CMUR243161:TC_0817-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33881; Mature: 33881

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLRVVYLGTPQFAATVLETLVDARIHVVGVVTRADKPQKRSSKPIASPVKQLALSKNI
CCEEEEEEEECCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHCCCCHHHHHHHHHHHCCC
PLLQPTKTTDPAFLAQLREWQADVFVVVAYGVILKQELLDIPKYGCYNLHAGLLPAYRGA
CCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCC
APIQRCIIAGETLSGNTVIRMDAGMDTGDIANVNHVAIGEDMTAGELAEALAGSGGELIL
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHH
KTLQEIEAGTVRHIPQDSAKATLAPKLTKEEGLVKWDAPASQVYAHIRGVSPAPGAWTRF
HHHHHHHCCHHCCCCCCCCCEEECCCCCCCCCCEEECCCHHHHHHHHCCCCCCCHHHHHH
LSQGKEPRRLGILSARMESSSGSHSPGEVLGVSGEDLLVACRQGVLRLCIVQPEGKVFMK
HHCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHCCEEEEEECCCCCEEEE
AKDFFNGQSRSVALLF
EHHHCCCCCCEEEEEC
>Mature Secondary Structure
MLNLRVVYLGTPQFAATVLETLVDARIHVVGVVTRADKPQKRSSKPIASPVKQLALSKNI
CCEEEEEEEECCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHCCCCHHHHHHHHHHHCCC
PLLQPTKTTDPAFLAQLREWQADVFVVVAYGVILKQELLDIPKYGCYNLHAGLLPAYRGA
CCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCC
APIQRCIIAGETLSGNTVIRMDAGMDTGDIANVNHVAIGEDMTAGELAEALAGSGGELIL
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHH
KTLQEIEAGTVRHIPQDSAKATLAPKLTKEEGLVKWDAPASQVYAHIRGVSPAPGAWTRF
HHHHHHHCCHHCCCCCCCCCEEECCCCCCCCCCEEECCCHHHHHHHHCCCCCCCHHHHHH
LSQGKEPRRLGILSARMESSSGSHSPGEVLGVSGEDLLVACRQGVLRLCIVQPEGKVFMK
HHCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHCCEEEEEECCCCCEEEE
AKDFFNGQSRSVALLF
EHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935