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The map label for this gene is rpoA

Identifier: 15835408

GI number: 15835408

Start: 936869

End: 938053

Strand: Reverse

Name: rpoA

Synonym: TC0794

Alternate gene names: 15835408

Gene position: 938053-936869 (Counterclockwise)

Preceding gene: 15835409

Following gene: 15835407

Centisome position: 87.43

GC content: 40.51

Gene sequence:

>1185_bases
GTGTCGACCAAGAAAACGACGAAGAGTGTAGTTATAGCTAAGGAGTTTGGGATGTCGGATAATTCACATAATTTACTTTA
TAACAAATTTGAGTTGCCTGAATCGGTGAAAATGTCTCCCGTTGAAGGTGCAGCTGGTGGCATTGATAAAGTAGCTCGGT
TTGTTGCTGATCCCTTAGAGAAGGGAATGGGGCACACATTGGGAAGCGCCTTGAGACGTGCTTTATTAATCGGCCTGGAA
GCTCCTGCTATTGTCTCTTTTTCTATGACAGGAGTTTTGCATGAATACATGGCGGTAGAGGGGATCATCGAGGATGTTAC
CAATATCGTTTTGAATTTGAAAGGCTCTTTGCTCAAAAAGTATCCTTTACAAGATTGTGAAGGAGGAAGAGCTTCCCAAA
AATTGCGAGCTACAATTTCTATCGATGCATCCGATTTAGCTGCTGCTGGTGGGCAAAAAGCAATTACTTTAGGGGATTTG
CTGCAAGAAGGCACCTTTGAAGCTGTAAATCCTGAGCATGTAATTTTTACAGTTACTCGTCCTATGCAACTAGATGTTAT
GTTGCGGGTTGCTTTCGGTAGAGGATATACTCCTTCCGAGAGAATTGTTCTCGAAGAAAGAGAGATGAATGAGATCGTTT
TAGATGCCGCCTTCTCTCCTGTTGTTTTGGTCAATTATTTCGTTGAAGATACTCGTGTTGGCCAGGATACGGATTTCGAT
CGTTTAATATTGCAAGTGGAAACGGATGGTCGTGTAGCTCCTAAAGAGGCTGTAGCTTTTGCTACGCAAATTTTGAGCAA
ACATTTTTCTGTTTTCGAAAAAATGGACGAGAAACGGATCGTTTTTGAAGAAGCCATTTCTGTAGAAAAAGAAAATAAAG
ACGATATTCTTCACAAGTTAGTTTTGGGCATTAATGAGATAGAACTCTCTGTACGATCCACAAACTGTTTGTCTAATGCC
AATATCGAAACGATAGGAGAGCTGGTAATTATGCCAGAGCCTCGCCTGTTACAATTTAGAAACTTCGGTAAGAAATCTCT
TTGTGAGATTAAGAATAAACTGAAAGAAATGAAATTAGAATTGGGCATGGATCTTAGCCAATTTGGCGTTGGGCTGGATA
ATGTCAAAGAAAAAATGAAGTGGTATGCCGAGAAAATTCGGTCGAGTAAGAATACCAAGGGATAA

Upstream 100 bases:

>100_bases
AAGGAACTGGTGCTGGGCGGGAATCTGCCGTACGAGCGCTTATTTCTTCTGGACTTATTGTTTCTGTTATCCGAGATGAA
ACTCCCGTTCCTCATAACGG

Downstream 100 bases:

>100_bases
AAAGGGATATGCAACACGCTAGAAAAAAATTTAGGGTTGGTCGTACTTCTTCGCATAACCGTTGCATGTTGGCTAATATG
TTAAAGTCTTTAATTCACAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSTKKTTKSVVIAKEFGMSDNSHNLLYNKFELPESVKMSPVEGAAGGIDKVARFVADPLEKGMGHTLGSALRRALLIGLE
APAIVSFSMTGVLHEYMAVEGIIEDVTNIVLNLKGSLLKKYPLQDCEGGRASQKLRATISIDASDLAAAGGQKAITLGDL
LQEGTFEAVNPEHVIFTVTRPMQLDVMLRVAFGRGYTPSERIVLEEREMNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFD
RLILQVETDGRVAPKEAVAFATQILSKHFSVFEKMDEKRIVFEEAISVEKENKDDILHKLVLGINEIELSVRSTNCLSNA
NIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLSQFGVGLDNVKEKMKWYAEKIRSSKNTKG

Sequences:

>Translated_394_residues
MSTKKTTKSVVIAKEFGMSDNSHNLLYNKFELPESVKMSPVEGAAGGIDKVARFVADPLEKGMGHTLGSALRRALLIGLE
APAIVSFSMTGVLHEYMAVEGIIEDVTNIVLNLKGSLLKKYPLQDCEGGRASQKLRATISIDASDLAAAGGQKAITLGDL
LQEGTFEAVNPEHVIFTVTRPMQLDVMLRVAFGRGYTPSERIVLEEREMNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFD
RLILQVETDGRVAPKEAVAFATQILSKHFSVFEKMDEKRIVFEEAISVEKENKDDILHKLVLGINEIELSVRSTNCLSNA
NIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLSQFGVGLDNVKEKMKWYAEKIRSSKNTKG
>Mature_393_residues
STKKTTKSVVIAKEFGMSDNSHNLLYNKFELPESVKMSPVEGAAGGIDKVARFVADPLEKGMGHTLGSALRRALLIGLEA
PAIVSFSMTGVLHEYMAVEGIIEDVTNIVLNLKGSLLKKYPLQDCEGGRASQKLRATISIDASDLAAAGGQKAITLGDLL
QEGTFEAVNPEHVIFTVTRPMQLDVMLRVAFGRGYTPSERIVLEEREMNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFDR
LILQVETDGRVAPKEAVAFATQILSKHFSVFEKMDEKRIVFEEAISVEKENKDDILHKLVLGINEIELSVRSTNCLSNAN
IETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLSQFGVGLDNVKEKMKWYAEKIRSSKNTKG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=321, Percent_Identity=38.3177570093458, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 43671; Mature: 43540

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKKTTKSVVIAKEFGMSDNSHNLLYNKFELPESVKMSPVEGAAGGIDKVARFVADPLE
CCCCCCHHHEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KGMGHTLGSALRRALLIGLEAPAIVSFSMTGVLHEYMAVEGIIEDVTNIVLNLKGSLLKK
HCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
YPLQDCEGGRASQKLRATISIDASDLAAAGGQKAITLGDLLQEGTFEAVNPEHVIFTVTR
CCCCCCCCCCCCCEEEEEEEECHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCEEEEEECC
PMQLDVMLRVAFGRGYTPSERIVLEEREMNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFD
CCEEHHEEEEHHCCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
RLILQVETDGRVAPKEAVAFATQILSKHFSVFEKMDEKRIVFEEAISVEKENKDDILHKL
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
VLGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLE
HHCCCHHEEEEECCCCCCCCCHHHHCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGMDLSQFGVGLDNVKEKMKWYAEKIRSSKNTKG
HCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STKKTTKSVVIAKEFGMSDNSHNLLYNKFELPESVKMSPVEGAAGGIDKVARFVADPLE
CCCCCHHHEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KGMGHTLGSALRRALLIGLEAPAIVSFSMTGVLHEYMAVEGIIEDVTNIVLNLKGSLLKK
HCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
YPLQDCEGGRASQKLRATISIDASDLAAAGGQKAITLGDLLQEGTFEAVNPEHVIFTVTR
CCCCCCCCCCCCCEEEEEEEECHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCEEEEEECC
PMQLDVMLRVAFGRGYTPSERIVLEEREMNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFD
CCEEHHEEEEHHCCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCC
RLILQVETDGRVAPKEAVAFATQILSKHFSVFEKMDEKRIVFEEAISVEKENKDDILHKL
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
VLGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLE
HHCCCHHEEEEECCCCCCCCCHHHHCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGMDLSQFGVGLDNVKEKMKWYAEKIRSSKNTKG
HCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]