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The map label for this gene is dnaB

Identifier: 15835398

GI number: 15835398

Start: 927596

End: 929014

Strand: Direct

Name: dnaB

Synonym: TC0784

Alternate gene names: 15835398

Gene position: 927596-929014 (Clockwise)

Preceding gene: 15835391

Following gene: 15835399

Centisome position: 86.45

GC content: 42.92

Gene sequence:

>1419_bases
ATGGCTACCCAAACAAAGAAACCCCACTCCCCCCAGCTTCTCTCCCTTCCTAATTCCAAGGAATCCGAAATGATTGTATT
GGGGTGCATGCTAACAAACGTCAATCACCTCAATCTTGCAGCAAACCTTTTGCAAGAAGAAGATTTCTACTTTTTGGAGC
ATCGCATTATTTTTCGCGTGCTGCAAGATGCCTTCAAATCAGATAGACCGATGGACCCTCATCTTACAGGGGAGGAACTA
AAACGTAGAGACCAACTTAACGTTATTGGGGGAGCCTCTTATCTCATCACGCTATCCGAATTTGCTGGGACTTCCGCCTA
TATTGAGGAATATGCGGATATTATCCGCTCCAAGTCAATTTTGAGAAAAATGATCCAAGCGGCCAAAGATATTGAAAAGA
AGGCCGCAGAAGAACCTCGTGATGTAACCACGGCTCTAGATGATGCTCAAAACTTATTGTTCCGCATAAGCCAAACAACA
AACTTTGCGCCTTACGTCCTTGTTTCGGACAAACTCAAAGGCCTTTCTTCTACTAAAGACAAATCCTTCTTGCTAGCGCT
ACAAGAACGTCAGGAAGCCTTTCAAGCTAGTTCCCATGATGCTAGAATCCCAACACTCTCAGGGTTCCCTACTCATTTTT
TGGACCTCGATAGGATGCTGAATGGATTCAGCCCCTCTAACCTTATTATTCTTGCTGCACGACCAGCTATGGGAAAAACT
GCCTTAGCTCTCAATATTGTAGAAAATTTTTGTTTTGAAAGCCGCCTACCAGTAGGCATTTTTTCTCTAGAGATGACCGT
GGACCAACTGATTCACCGAATCATTTGCTCTCGCTCCGAAGTCGAAGCCAAGAAAATTAGTGTGGGAGACATCTCTGGTC
GAGATTTTCAACGTGTAGTCTCTGTGGTCCGAGAAATGGAAGAACATACTTTACTAATAGACGACTATCCGGGATTAAAA
ATCACTGATCTCCGAGCTCGAGCTCGAAGAATGAAAGAAAGCTACGACATCCAATTTCTTGTTATTGACTACCTACAACT
GATCTCAAGTTCTGGGAACTTAAGGAATTCTGATTCACGTAACCAAGAAATTTCTGAAATTTCCCGTATGCTTAAAAATT
TGGCTAGGGAACTCAATATCCCCATCCTTTGTCTCTCCCAATTATCAAGAAAAGTGGAAGACCGAGCCAATCATAGGCCC
TTGATGAGCGATTTGCGAGAGAGTGGAAGCATTGAACAGGATGCCGACCAAATTATGTTTTTACTCCGTCGCGAATACTA
TGATCCTAACGACAAACCTGGAACAGCAGAGTTGATTGTTGCTAAAAATCGACATGGATCGATTGGATCTGTCCAATTAG
TTTTTGAAAAAGACTTTGCTCGATTCCGAAATTATGCTGGATGTGAATTCCCAGGCTAA

Upstream 100 bases:

>100_bases
GATATTTTTCTATCCTCTTTCCTCTTGAGGATATATCACACGATCCCCTACCCTGACCGAGAGAGCTCCATCCACCAATA
ATAAAAACAAACCTATAACT

Downstream 100 bases:

>100_bases
ACAAGTTTATTCCCAAAAATTGATCACCATTGTAAACTTCTGCCCATCGCTGGTGGAAACCAGAGATGCGCTTTGAGAAA
AGTCCAGAGGGAATTGACTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MATQTKKPHSPQLLSLPNSKESEMIVLGCMLTNVNHLNLAANLLQEEDFYFLEHRIIFRVLQDAFKSDRPMDPHLTGEEL
KRRDQLNVIGGASYLITLSEFAGTSAYIEEYADIIRSKSILRKMIQAAKDIEKKAAEEPRDVTTALDDAQNLLFRISQTT
NFAPYVLVSDKLKGLSSTKDKSFLLALQERQEAFQASSHDARIPTLSGFPTHFLDLDRMLNGFSPSNLIILAARPAMGKT
ALALNIVENFCFESRLPVGIFSLEMTVDQLIHRIICSRSEVEAKKISVGDISGRDFQRVVSVVREMEEHTLLIDDYPGLK
ITDLRARARRMKESYDIQFLVIDYLQLISSSGNLRNSDSRNQEISEISRMLKNLARELNIPILCLSQLSRKVEDRANHRP
LMSDLRESGSIEQDADQIMFLLRREYYDPNDKPGTAELIVAKNRHGSIGSVQLVFEKDFARFRNYAGCEFPG

Sequences:

>Translated_472_residues
MATQTKKPHSPQLLSLPNSKESEMIVLGCMLTNVNHLNLAANLLQEEDFYFLEHRIIFRVLQDAFKSDRPMDPHLTGEEL
KRRDQLNVIGGASYLITLSEFAGTSAYIEEYADIIRSKSILRKMIQAAKDIEKKAAEEPRDVTTALDDAQNLLFRISQTT
NFAPYVLVSDKLKGLSSTKDKSFLLALQERQEAFQASSHDARIPTLSGFPTHFLDLDRMLNGFSPSNLIILAARPAMGKT
ALALNIVENFCFESRLPVGIFSLEMTVDQLIHRIICSRSEVEAKKISVGDISGRDFQRVVSVVREMEEHTLLIDDYPGLK
ITDLRARARRMKESYDIQFLVIDYLQLISSSGNLRNSDSRNQEISEISRMLKNLARELNIPILCLSQLSRKVEDRANHRP
LMSDLRESGSIEQDADQIMFLLRREYYDPNDKPGTAELIVAKNRHGSIGSVQLVFEKDFARFRNYAGCEFPG
>Mature_471_residues
ATQTKKPHSPQLLSLPNSKESEMIVLGCMLTNVNHLNLAANLLQEEDFYFLEHRIIFRVLQDAFKSDRPMDPHLTGEELK
RRDQLNVIGGASYLITLSEFAGTSAYIEEYADIIRSKSILRKMIQAAKDIEKKAAEEPRDVTTALDDAQNLLFRISQTTN
FAPYVLVSDKLKGLSSTKDKSFLLALQERQEAFQASSHDARIPTLSGFPTHFLDLDRMLNGFSPSNLIILAARPAMGKTA
LALNIVENFCFESRLPVGIFSLEMTVDQLIHRIICSRSEVEAKKISVGDISGRDFQRVVSVVREMEEHTLLIDDYPGLKI
TDLRARARRMKESYDIQFLVIDYLQLISSSGNLRNSDSRNQEISEISRMLKNLARELNIPILCLSQLSRKVEDRANHRPL
MSDLRESGSIEQDADQIMFLLRREYYDPNDKPGTAELIVAKNRHGSIGSVQLVFEKDFARFRNYAGCEFPG

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=468, Percent_Identity=40.3846153846154, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53535; Mature: 53404

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQTKKPHSPQLLSLPNSKESEMIVLGCMLTNVNHLNLAANLLQEEDFYFLEHRIIFRV
CCCCCCCCCCCCEEECCCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHH
LQDAFKSDRPMDPHLTGEELKRRDQLNVIGGASYLITLSEFAGTSAYIEEYADIIRSKSI
HHHHHHCCCCCCCCCCHHHHHHHHCCCEEECCHHEEEEHHHCCCHHHHHHHHHHHHHHHH
LRKMIQAAKDIEKKAAEEPRDVTTALDDAQNLLFRISQTTNFAPYVLVSDKLKGLSSTKD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCCCCCCC
KSFLLALQERQEAFQASSHDARIPTLSGFPTHFLDLDRMLNGFSPSNLIILAARPAMGKT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCH
ALALNIVENFCFESRLPVGIFSLEMTVDQLIHRIICSRSEVEAKKISVGDISGRDFQRVV
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCHHHHHHH
SVVREMEEHTLLIDDYPGLKITDLRARARRMKESYDIQFLVIDYLQLISSSGNLRNSDSR
HHHHHHHHCEEEEECCCCCEEEHHHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCCCCCC
NQEISEISRMLKNLARELNIPILCLSQLSRKVEDRANHRPLMSDLRESGSIEQDADQIMF
HHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHH
LLRREYYDPNDKPGTAELIVAKNRHGSIGSVQLVFEKDFARFRNYAGCEFPG
HHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ATQTKKPHSPQLLSLPNSKESEMIVLGCMLTNVNHLNLAANLLQEEDFYFLEHRIIFRV
CCCCCCCCCCCEEECCCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHH
LQDAFKSDRPMDPHLTGEELKRRDQLNVIGGASYLITLSEFAGTSAYIEEYADIIRSKSI
HHHHHHCCCCCCCCCCHHHHHHHHCCCEEECCHHEEEEHHHCCCHHHHHHHHHHHHHHHH
LRKMIQAAKDIEKKAAEEPRDVTTALDDAQNLLFRISQTTNFAPYVLVSDKLKGLSSTKD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHCCCCCCCC
KSFLLALQERQEAFQASSHDARIPTLSGFPTHFLDLDRMLNGFSPSNLIILAARPAMGKT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCH
ALALNIVENFCFESRLPVGIFSLEMTVDQLIHRIICSRSEVEAKKISVGDISGRDFQRVV
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHEEECCCCCHHHHHHH
SVVREMEEHTLLIDDYPGLKITDLRARARRMKESYDIQFLVIDYLQLISSSGNLRNSDSR
HHHHHHHHCEEEEECCCCCEEEHHHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCCCCCC
NQEISEISRMLKNLARELNIPILCLSQLSRKVEDRANHRPLMSDLRESGSIEQDADQIMF
HHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHH
LLRREYYDPNDKPGTAELIVAKNRHGSIGSVQLVFEKDFARFRNYAGCEFPG
HHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]