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The map label for this gene is rho

Identifier: 15835392

GI number: 15835392

Start: 919008

End: 920267

Strand: Reverse

Name: rho

Synonym: TC0778

Alternate gene names: 15835392

Gene position: 920267-919008 (Counterclockwise)

Preceding gene: 15835393

Following gene: 15835390

Centisome position: 85.77

GC content: 42.3

Gene sequence:

>1260_bases
GTGGCAGTGACAAAGATTGCTAAACTACAACGCATGGGCATTAATGAGCTGAATGTTTTAGCGCGGCAATATGGGGTGAA
GAATGTAGGTTCCCTGACGAAATCGCAGGTAGTGTTCGAAATCGTTAAAGCTAAGTCTGAACGTCCTGATGAGTTTTTGA
TTGGTGAGGGAGTTTTAGAAGTTCTCCCAGATGGCTTTGGGTTTCTGAGATCTCCTACATACAACTATCTTCCTTCTGCA
GAGGATATTTATGTTTCTCCAGCGCAGATTCGTCGTTTTGATTTGAAGAAAGGAGATACTATCGTTGGAACGATCCGTTC
TCCTAAGGAGAAAGAGAAGTATTTTGCTTTATTGAAAGTAGATAAAATCAACGGTTCGACTCCCGATAAAGCGAAAGAAA
GAGTTTTATTTGAGAACTTAACCCCACTGCATCCTAATGAGCGGTTAATCATGGAGATGGGGAAAGAAAACCTGGCGGAG
CGTGTATTGGATTTAACAGCTCCAATTGGTAAGGGACAACGAGGGCTGATTGTGGCTCCGCCTCGTTCCGGGAAAACAGT
GATTTTGCAGAGTATTGCGCACGCCATTGCTGTAAATAATCCTGATGCAGAGCTCATTGTATTACTGATTGATGAGCGTC
CTGAAGAAGTTACTGATATGATTCGTCAGGTTCGAGGTGAAGTTGTTGCATCGACTTTTGATGAACAGCCTGATCGACAT
ATTCAAGTTGCTGAAATGGTAATTGAAAAGGCAAGACGTTTAGTTGAGCACGGTAAAGATGTTGTGATTTTACTAGATTC
TATTACGCGTTTAGCTCGTGCATATAACACTGTGCAACCACATTCAGGAAAAATCCTCACTGGAGGGGTAGATGCCAGTG
CCTTACATAAACCTAAGCGTTTCTTTGGTGCGGCTAGAAATATTGAAGGTGGTGGGTCTCTAACGATTTTAGCCACAGCG
TTAATTGATACGGGGTCAAGAATGGATGAGGTGATCTTTGAGGAATTCAAAGGAACCGGAAACATGGAATTAGTTCTTGA
TCGGCACCTTTCTGATCGTAGAATTTATCCTGCTATTGATTTGATTAAGAGCGGAACACGAAAAGAAGAATTGCTATACC
ATCCAGGAGAGTTAGAGAAAGTGCGGCTGTTCCGGCAGGCTATAGCTGGCTTAACAGCAATAGATGCAATGCAGTTGCTT
CTTGGTCGGTTGAAAAAGACAAATAGCAACACAGAGTTTCTATTGTCATTAAAGGATTAA

Upstream 100 bases:

>100_bases
GGTAAAGGAGCATAAGAGGCGCGAGGGTCCTTTACCATTGGGAAAAGAAGGTAGTGAAGACAGTGCTGTTGCTACAGAAG
AGAAAGAGGCCCCGCGGCCA

Downstream 100 bases:

>100_bases
AAGAAGTTTTTTATCCAATTAGGACTTCTGGAATGATGACGGGGGTGGCTTGGTAGGTTTGAATATTCCGAAAGGCCACC
CCGTTTAATTTTAGTAGAGA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAVTKIAKLQRMGINELNVLARQYGVKNVGSLTKSQVVFEIVKAKSERPDEFLIGEGVLEVLPDGFGFLRSPTYNYLPSA
EDIYVSPAQIRRFDLKKGDTIVGTIRSPKEKEKYFALLKVDKINGSTPDKAKERVLFENLTPLHPNERLIMEMGKENLAE
RVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHAIAVNNPDAELIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPDRH
IQVAEMVIEKARRLVEHGKDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATA
LIDTGSRMDEVIFEEFKGTGNMELVLDRHLSDRRIYPAIDLIKSGTRKEELLYHPGELEKVRLFRQAIAGLTAIDAMQLL
LGRLKKTNSNTEFLLSLKD

Sequences:

>Translated_419_residues
MAVTKIAKLQRMGINELNVLARQYGVKNVGSLTKSQVVFEIVKAKSERPDEFLIGEGVLEVLPDGFGFLRSPTYNYLPSA
EDIYVSPAQIRRFDLKKGDTIVGTIRSPKEKEKYFALLKVDKINGSTPDKAKERVLFENLTPLHPNERLIMEMGKENLAE
RVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHAIAVNNPDAELIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPDRH
IQVAEMVIEKARRLVEHGKDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATA
LIDTGSRMDEVIFEEFKGTGNMELVLDRHLSDRRIYPAIDLIKSGTRKEELLYHPGELEKVRLFRQAIAGLTAIDAMQLL
LGRLKKTNSNTEFLLSLKD
>Mature_418_residues
AVTKIAKLQRMGINELNVLARQYGVKNVGSLTKSQVVFEIVKAKSERPDEFLIGEGVLEVLPDGFGFLRSPTYNYLPSAE
DIYVSPAQIRRFDLKKGDTIVGTIRSPKEKEKYFALLKVDKINGSTPDKAKERVLFENLTPLHPNERLIMEMGKENLAER
VLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHAIAVNNPDAELIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPDRHI
QVAEMVIEKARRLVEHGKDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATAL
IDTGSRMDEVIFEEFKGTGNMELVLDRHLSDRRIYPAIDLIKSGTRKEELLYHPGELEKVRLFRQAIAGLTAIDAMQLLL
GRLKKTNSNTEFLLSLKD

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=61.5384615384615, Blast_Score=519, Evalue=1e-148,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 46601; Mature: 46470

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTKIAKLQRMGINELNVLARQYGVKNVGSLTKSQVVFEIVKAKSERPDEFLIGEGVLE
CCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHH
VLPDGFGFLRSPTYNYLPSAEDIYVSPAQIRRFDLKKGDTIVGTIRSPKEKEKYFALLKV
HCCCCCHHHCCCCCCCCCCCCCEEECHHHHHHEECCCCCEEEEECCCCHHHHHEEEEEEE
DKINGSTPDKAKERVLFENLTPLHPNERLIMEMGKENLAERVLDLTAPIGKGQRGLIVAP
EECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEEC
PRSGKTVILQSIAHAIAVNNPDAELIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPDRH
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
IQVAEMVIEKARRLVEHGKDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKR
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCHH
FFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMELVLDRHLSDRRIYPAID
HHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
LIKSGTRKEELLYHPGELEKVRLFRQAIAGLTAIDAMQLLLGRLKKTNSNTEFLLSLKD
HHHCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure 
AVTKIAKLQRMGINELNVLARQYGVKNVGSLTKSQVVFEIVKAKSERPDEFLIGEGVLE
CHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHH
VLPDGFGFLRSPTYNYLPSAEDIYVSPAQIRRFDLKKGDTIVGTIRSPKEKEKYFALLKV
HCCCCCHHHCCCCCCCCCCCCCEEECHHHHHHEECCCCCEEEEECCCCHHHHHEEEEEEE
DKINGSTPDKAKERVLFENLTPLHPNERLIMEMGKENLAERVLDLTAPIGKGQRGLIVAP
EECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEEC
PRSGKTVILQSIAHAIAVNNPDAELIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPDRH
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
IQVAEMVIEKARRLVEHGKDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKR
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCHH
FFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMELVLDRHLSDRRIYPAID
HHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
LIKSGTRKEELLYHPGELEKVRLFRQAIAGLTAIDAMQLLLGRLKKTNSNTEFLLSLKD
HHHCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]