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The map label for this gene is ribE

Identifier: 15835300

GI number: 15835300

Start: 817813

End: 818409

Strand: Reverse

Name: ribE

Synonym: TC0685

Alternate gene names: 15835300

Gene position: 818409-817813 (Counterclockwise)

Preceding gene: 15835314

Following gene: 15835299

Centisome position: 76.28

GC content: 41.54

Gene sequence:

>597_bases
ATGTTTTCAGGCATTATTCAAGAGGTAGCACGTGTAGACCTTATTCATCATTACGGGGATTCCATGGAGATAGGAATTTT
TGCACGTAATCTAGTAGATGGGGTTCCGGGAAGTAGTATCGCTGTTGATGGGATCTGTTTGACTTTAGTCAAAAGAGAAT
TCGAATTACTATTTTTTGATGTGACAGAAGAGACCATGGCTTGTACCACGATTAAAAATTACACCGTGGGGAGTATGGTG
AATTTAGAGCGTTCTGTTCGATTAGGGGATGAAATAGGTGGGCATTTTGTTTCTGGTCATGTCTGTGGTGTGGGTACCAT
CATTGCCGTAGAAAAATCTTATATGTTTTTTAAGGCTCCAACAAATTTGGTGCCTTATGTACTCGAAAAAGGCTTTATCG
CTATTGATGGTATTAGTTTGACGATCGCTCAGGTAAGGGGAGATATCTTTTCCGTCAGCGTGATTCCTGAAACTCGAGCT
CGGACTTCTTTGGGTTACAAACAGGTTGGTTCTCACGTAAATATGGAGCCCGATATGATGACAAAAATGCAGGTGGATAC
AGTTATGCGGTTCCAGGCCGAAAAGATAGGCAAATGA

Upstream 100 bases:

>100_bases
TAGTTTGCCTCAATGTGATCCAGAGGCTTTTTCGTGTATCTAGTTTCTTAAGTTACTAAGAATTATTTTGTCACAGCCTA
GCAAAGGTGTTTGGTTATCC

Downstream 100 bases:

>100_bases
TGGATTACGAATTATTAGATAGCGGGGATGGTAAGAAACTTGAGCGATTTAAGGATGTTGTTTTAATTCGTCCTTCTGCT
ACGGCTATTTGGCCAAAAAC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MFSGIIQEVARVDLIHHYGDSMEIGIFARNLVDGVPGSSIAVDGICLTLVKREFELLFFDVTEETMACTTIKNYTVGSMV
NLERSVRLGDEIGGHFVSGHVCGVGTIIAVEKSYMFFKAPTNLVPYVLEKGFIAIDGISLTIAQVRGDIFSVSVIPETRA
RTSLGYKQVGSHVNMEPDMMTKMQVDTVMRFQAEKIGK

Sequences:

>Translated_198_residues
MFSGIIQEVARVDLIHHYGDSMEIGIFARNLVDGVPGSSIAVDGICLTLVKREFELLFFDVTEETMACTTIKNYTVGSMV
NLERSVRLGDEIGGHFVSGHVCGVGTIIAVEKSYMFFKAPTNLVPYVLEKGFIAIDGISLTIAQVRGDIFSVSVIPETRA
RTSLGYKQVGSHVNMEPDMMTKMQVDTVMRFQAEKIGK
>Mature_198_residues
MFSGIIQEVARVDLIHHYGDSMEIGIFARNLVDGVPGSSIAVDGICLTLVKREFELLFFDVTEETMACTTIKNYTVGSMV
NLERSVRLGDEIGGHFVSGHVCGVGTIIAVEKSYMFFKAPTNLVPYVLEKGFIAIDGISLTIAQVRGDIFSVSVIPETRA
RTSLGYKQVGSHVNMEPDMMTKMQVDTVMRFQAEKIGK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats

Homologues:

Organism=Escherichia coli, GI1787952, Length=202, Percent_Identity=39.1089108910891, Blast_Score=131, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319733, Length=200, Percent_Identity=33, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISA_CHLMU (Q9PJZ1)

Other databases:

- EMBL:   AE002160
- PIR:   G81675
- RefSeq:   NP_297059.1
- ProteinModelPortal:   Q9PJZ1
- SMR:   Q9PJZ1
- GeneID:   1246046
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0685
- TIGR:   TC_0685
- HOGENOM:   HBG289809
- OMA:   CCLTVTK
- PhylomeDB:   Q9PJZ1
- ProtClustDB:   PRK09289
- BioCyc:   CMUR243161:TC_0685-MONOMER
- BRENDA:   2.5.1.9
- InterPro:   IPR001783
- InterPro:   IPR017938
- PANTHER:   PTHR21098
- PIRSF:   PIRSF000498
- TIGRFAMs:   TIGR00187

Pfam domain/function: PF00677 Lum_binding; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =2.5.1.9

Molecular weight: Translated: 21762; Mature: 21762

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS51177 LUMAZINE_BIND

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGIIQEVARVDLIHHYGDSMEIGIFARNLVDGVPGSSIAVDGICLTLVKREFELLFFD
CCHHHHHHHHHHHHHHHCCCCEEEHEEEHHHHCCCCCCCEEHHHHHHHHHHHCEEEEEEE
VTEETMACTTIKNYTVGSMVNLERSVRLGDEIGGHFVSGHVCGVGTIIAVEKSYMFFKAP
ECCCCEEEEEECCCCCCCEECHHHHHHCCHHHCCEEEECEECCCEEEEEEEECEEEEECC
TNLVPYVLEKGFIAIDGISLTIAQVRGDIFSVSVIPETRARTSLGYKQVGSHVNMEPDMM
CCHHHHHHHCCEEEECCCEEEEEECCCCEEEEEECCCCHHHHHCCHHHHCCCCCCCCHHH
TKMQVDTVMRFQAEKIGK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSGIIQEVARVDLIHHYGDSMEIGIFARNLVDGVPGSSIAVDGICLTLVKREFELLFFD
CCHHHHHHHHHHHHHHHCCCCEEEHEEEHHHHCCCCCCCEEHHHHHHHHHHHCEEEEEEE
VTEETMACTTIKNYTVGSMVNLERSVRLGDEIGGHFVSGHVCGVGTIIAVEKSYMFFKAP
ECCCCEEEEEECCCCCCCEECHHHHHHCCHHHCCEEEECEECCCEEEEEEEECEEEEECC
TNLVPYVLEKGFIAIDGISLTIAQVRGDIFSVSVIPETRARTSLGYKQVGSHVNMEPDMM
CCHHHHHHHCCEEEECCCEEEEEECCCCEEEEEECCCCHHHHHCCHHHHCCCCCCCCHHH
TKMQVDTVMRFQAEKIGK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935