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Identifier: 15835299

GI number: 15835299

Start: 816989

End: 817816

Strand: Reverse

Name: Not Available

Synonym: TC0684

Alternate gene names: 15835299

Gene position: 817816-816989 (Counterclockwise)

Preceding gene: 15835300

Following gene: 15835298

Centisome position: 76.22

GC content: 40.82

Gene sequence:

>828_bases
ATGATGGATTACGAATTATTAGATAGCGGGGATGGTAAGAAACTTGAGCGATTTAAGGATGTTGTTTTAATTCGTCCTTC
TGCTACGGCTATTTGGCCAAAAACTTCACCAGCTTTGTGGAAACAATATTCTGCTGAGTTTGTTCGTGTTGGGGAGAAAG
GAGAGTGGAAATACCGCAATTCACATCTTAAGGAATGGTGGATAACTATTGATTCTGTGTCATGCTTGTTGAAGCTTACT
CCCTTTGGTCATGTGGGGATTTTCCCTGAGCATGCTACATTCTGGCAAGATCTAAGCCCCTCTTTATCGAAGCCTTCTTG
TAAGGTCTTGAATTTATTTGCTTATACTGGAGCTTCTTCTATTTTTTGTGCCCAGCAGGGGGCTGCTGTCTATCATGTAG
ATGCTTCTAAGGCTGCTGTAAAATGGGCTCAAAAAAATGTGGAAGGGAATTCTTTTTCAGAAAAAAGAATTTTTTGGATC
ATAGAAGATGTTTTTTCATTTCTAAAAAAAGAAATTCGTAGGGGAAAAACCTATGATGTGATTCTTTTGGACCCCCCAAC
TTATGGACGAGGTCCTGATGGAGAAACCTTTAAAATAGATCGGGATTTTTTCCCATTATTGGAGGCCTGTTCACAGCTGC
TTTCTAGTTCTTTTTCGCATATGTTGATTACTTCGCATACGCCAGGACATACCCCTGAATTTTTGCATTGCTTAGCTAAA
AGGGCGCTTCCTATGCTTTCTTCCCAAAAATGGCGGTTGGGAGAGAATTTTTGTGGTGAGGGAGATCAACGGTTGCCTTC
GGGAGTTTTTGCGCAATGGAGTTTATAG

Upstream 100 bases:

>100_bases
GTTACAAACAGGTTGGTTCTCACGTAAATATGGAGCCCGATATGATGACAAAAATGCAGGTGGATACAGTTATGCGGTTC
CAGGCCGAAAAGATAGGCAA

Downstream 100 bases:

>100_bases
GAAAAAATAATGCTCGTGTTAAAGCGGCTCTTGCTTTAAAGCGACAGCGAGCACGTAATAGTTCATACTTTTTATTAGAA
GGGTTCCGCGAGATTCACCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MMDYELLDSGDGKKLERFKDVVLIRPSATAIWPKTSPALWKQYSAEFVRVGEKGEWKYRNSHLKEWWITIDSVSCLLKLT
PFGHVGIFPEHATFWQDLSPSLSKPSCKVLNLFAYTGASSIFCAQQGAAVYHVDASKAAVKWAQKNVEGNSFSEKRIFWI
IEDVFSFLKKEIRRGKTYDVILLDPPTYGRGPDGETFKIDRDFFPLLEACSQLLSSSFSHMLITSHTPGHTPEFLHCLAK
RALPMLSSQKWRLGENFCGEGDQRLPSGVFAQWSL

Sequences:

>Translated_275_residues
MMDYELLDSGDGKKLERFKDVVLIRPSATAIWPKTSPALWKQYSAEFVRVGEKGEWKYRNSHLKEWWITIDSVSCLLKLT
PFGHVGIFPEHATFWQDLSPSLSKPSCKVLNLFAYTGASSIFCAQQGAAVYHVDASKAAVKWAQKNVEGNSFSEKRIFWI
IEDVFSFLKKEIRRGKTYDVILLDPPTYGRGPDGETFKIDRDFFPLLEACSQLLSSSFSHMLITSHTPGHTPEFLHCLAK
RALPMLSSQKWRLGENFCGEGDQRLPSGVFAQWSL
>Mature_275_residues
MMDYELLDSGDGKKLERFKDVVLIRPSATAIWPKTSPALWKQYSAEFVRVGEKGEWKYRNSHLKEWWITIDSVSCLLKLT
PFGHVGIFPEHATFWQDLSPSLSKPSCKVLNLFAYTGASSIFCAQQGAAVYHVDASKAAVKWAQKNVEGNSFSEKRIFWI
IEDVFSFLKKEIRRGKTYDVILLDPPTYGRGPDGETFKIDRDFFPLLEACSQLLSSSFSHMLITSHTPGHTPEFLHCLAK
RALPMLSSQKWRLGENFCGEGDQRLPSGVFAQWSL

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=251, Percent_Identity=23.9043824701195, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 31234; Mature: 31234

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDYELLDSGDGKKLERFKDVVLIRPSATAIWPKTSPALWKQYSAEFVRVGEKGEWKYRN
CCCCCCCCCCCCHHHHHHCCEEEECCCCCEECCCCCHHHHHHHCCHHEEECCCCCCCHHH
SHLKEWWITIDSVSCLLKLTPFGHVGIFPEHATFWQDLSPSLSKPSCKVLNLFAYTGASS
CCCEEEEEEEECEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCE
IFCAQQGAAVYHVDASKAAVKWAQKNVEGNSFSEKRIFWIIEDVFSFLKKEIRRGKTYDV
EEEECCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCEEE
ILLDPPTYGRGPDGETFKIDRDFFPLLEACSQLLSSSFSHMLITSHTPGHTPEFLHCLAK
EEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
RALPMLSSQKWRLGENFCGEGDQRLPSGVFAQWSL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MMDYELLDSGDGKKLERFKDVVLIRPSATAIWPKTSPALWKQYSAEFVRVGEKGEWKYRN
CCCCCCCCCCCCHHHHHHCCEEEECCCCCEECCCCCHHHHHHHCCHHEEECCCCCCCHHH
SHLKEWWITIDSVSCLLKLTPFGHVGIFPEHATFWQDLSPSLSKPSCKVLNLFAYTGASS
CCCEEEEEEEECEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCE
IFCAQQGAAVYHVDASKAAVKWAQKNVEGNSFSEKRIFWIIEDVFSFLKKEIRRGKTYDV
EEEECCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCEEE
ILLDPPTYGRGPDGETFKIDRDFFPLLEACSQLLSSSFSHMLITSHTPGHTPEFLHCLAK
EEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
RALPMLSSQKWRLGENFCGEGDQRLPSGVFAQWSL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA