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The map label for this gene is lpxK

Identifier: 15835297

GI number: 15835297

Start: 814899

End: 816008

Strand: Reverse

Name: lpxK

Synonym: TC0682

Alternate gene names: 15835297

Gene position: 816008-814899 (Counterclockwise)

Preceding gene: 15835298

Following gene: 15835296

Centisome position: 76.05

GC content: 43.51

Gene sequence:

>1110_bases
ATGAGATTTAGTTTCTTGTCTGGAATTCGAGACCTTTTCCGTCATTTCATCATCTCTGCGGCTTCTGGGGCTTTGTCTGA
TCGTTTGGGCTGGGTTTGGGGAGCTATTGCCAAGGTGTTTTCCGGTTCCGTTTGGCTGCGATATAAGATTGCAAAACCTC
CTCATCAGGTACAGGCCACGGTTGTAAGCGTAGGGAATATTGTTGTCGGAGGGACTGGAAAGACACCCTTGGTACTGTGG
TTAGCGCAGGCTTTAAATGAGAGGGGGATTTCTTGCGCTGTTTTATCAAGAGGGTACAAAGGGAAGTGTAGCCAAAGGAA
GTCGTTTACTATTGTAGATCCCGCGCTACATACAGCGGCATGTGTTGGTGATGAGCCATTGCTTTTAGCCAAGCATTTGC
CAGCGGGAACTGTGAGAATACAAAAAGACCGTAAAGCTCTTGCTGAAAAAAGTGCAGGGGCCTTCGATGTATTGTTATTA
GACGATGGTTTTCAATGCAATCGTCTTCATAAGGATGTAGAGATTGTATTGGTGAATGGATCGGATCCTTTTGGTGGAAG
AGCCTTTTTCCCAAAAGGGAGGCTACGGGACTTTCCAGAAAGATTAGCTAAGGCGAATTACGTAATTGTTAACGGTAAGT
GCTCACCATCCGATCAGCGTGAGTTAGATCTATTAAACCCTGCAGCCAAAATTCTTGTTGAACCGCAGATTTCGGAAATT
GTGTGGTTGAACAAGTCTGCAAATATGCCTCGAGATCATTGGGAAGGTTTAGGAGTAGGGGTTTTTTGTGGTTTAGGTTT
TCCTAAAGGGTTTTTGACTATGTTGAGAAATGCGGGAATCCATGTTTTAGGGACGCATTTATTGCCGGATCATTCGGGAA
TAACAAAACAAGAGTTAGAACTTTTTTGTAAGAAAATTATTTTGCGCCAAGGAGTTGGTATCCTATGCACAGAAAAGGAT
AGCGTAAAAATAGAAGCTTTCGCTGAGGAAATGTCATTGCCAATAGGAGAAGTCCGTATGCGGTTTTCTTGTGTGTGTAA
TGAAGAACGAATGGTAGCCATGTTGGATGCAATAGAAGCCATCCAGAAGAATAAGAAGGTGACTACATGA

Upstream 100 bases:

>100_bases
TGTTTAGGTAAAGGGGACGAAGAGTCTGTCTCAGCCCTTTAGGGTTGTCGTAGTTTTTGCAGGTTGTTCGTTTAGGAAGA
ACTTGAGTAAAGAGGCACCC

Downstream 100 bases:

>100_bases
AATTATGGATGAAAATCTTTATAGGCCTAATCATCGGAGTTGTTTCTGGCTTGGTTCTAGAAGACAAAGCAGTTTTTGTT
AAGCCTTTTGGGGATATATT

Product: tetraacyldisaccharide 4'-kinase

Products: NA

Alternate protein names: Lipid A 4'-kinase

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MRFSFLSGIRDLFRHFIISAASGALSDRLGWVWGAIAKVFSGSVWLRYKIAKPPHQVQATVVSVGNIVVGGTGKTPLVLW
LAQALNERGISCAVLSRGYKGKCSQRKSFTIVDPALHTAACVGDEPLLLAKHLPAGTVRIQKDRKALAEKSAGAFDVLLL
DDGFQCNRLHKDVEIVLVNGSDPFGGRAFFPKGRLRDFPERLAKANYVIVNGKCSPSDQRELDLLNPAAKILVEPQISEI
VWLNKSANMPRDHWEGLGVGVFCGLGFPKGFLTMLRNAGIHVLGTHLLPDHSGITKQELELFCKKIILRQGVGILCTEKD
SVKIEAFAEEMSLPIGEVRMRFSCVCNEERMVAMLDAIEAIQKNKKVTT

Sequences:

>Translated_369_residues
MRFSFLSGIRDLFRHFIISAASGALSDRLGWVWGAIAKVFSGSVWLRYKIAKPPHQVQATVVSVGNIVVGGTGKTPLVLW
LAQALNERGISCAVLSRGYKGKCSQRKSFTIVDPALHTAACVGDEPLLLAKHLPAGTVRIQKDRKALAEKSAGAFDVLLL
DDGFQCNRLHKDVEIVLVNGSDPFGGRAFFPKGRLRDFPERLAKANYVIVNGKCSPSDQRELDLLNPAAKILVEPQISEI
VWLNKSANMPRDHWEGLGVGVFCGLGFPKGFLTMLRNAGIHVLGTHLLPDHSGITKQELELFCKKIILRQGVGILCTEKD
SVKIEAFAEEMSLPIGEVRMRFSCVCNEERMVAMLDAIEAIQKNKKVTT
>Mature_369_residues
MRFSFLSGIRDLFRHFIISAASGALSDRLGWVWGAIAKVFSGSVWLRYKIAKPPHQVQATVVSVGNIVVGGTGKTPLVLW
LAQALNERGISCAVLSRGYKGKCSQRKSFTIVDPALHTAACVGDEPLLLAKHLPAGTVRIQKDRKALAEKSAGAFDVLLL
DDGFQCNRLHKDVEIVLVNGSDPFGGRAFFPKGRLRDFPERLAKANYVIVNGKCSPSDQRELDLLNPAAKILVEPQISEI
VWLNKSANMPRDHWEGLGVGVFCGLGFPKGFLTMLRNAGIHVLGTHLLPDHSGITKQELELFCKKIILRQGVGILCTEKD
SVKIEAFAEEMSLPIGEVRMRFSCVCNEERMVAMLDAIEAIQKNKKVTT

Specific function: Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1- P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA)

COG id: COG1663

COG function: function code M; Tetraacyldisaccharide-1-P 4'-kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxK family

Homologues:

Organism=Escherichia coli, GI1787144, Length=307, Percent_Identity=32.5732899022801, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXK_CHLMU (Q9PJZ4)

Other databases:

- EMBL:   AE002160
- PIR:   E81675
- RefSeq:   NP_297056.1
- GeneID:   1246043
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0682
- TIGR:   TC_0682
- HOGENOM:   HBG414064
- OMA:   DEPVLIH
- PhylomeDB:   Q9PJZ4
- ProtClustDB:   PRK00652
- BioCyc:   CMUR243161:TC_0682-MONOMER
- BRENDA:   2.7.1.130
- HAMAP:   MF_00409
- InterPro:   IPR003758
- TIGRFAMs:   TIGR00682

Pfam domain/function: PF02606 LpxK

EC number: =2.7.1.130

Molecular weight: Translated: 40500; Mature: 40500

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSFLSGIRDLFRHFIISAASGALSDRLGWVWGAIAKVFSGSVWLRYKIAKPPHQVQAT
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHEE
VVSVGNIVVGGTGKTPLVLWLAQALNERGISCAVLSRGYKGKCSQRKSFTIVDPALHTAA
EEEECCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECHHHHHHH
CVGDEPLLLAKHLPAGTVRIQKDRKALAEKSAGAFDVLLLDDGFQCNRLHKDVEIVLVNG
HCCCCCEEEEECCCCCEEEEEHHHHHHHHCCCCCEEEEEECCCCCHHCCCCCEEEEEEEC
SDPFGGRAFFPKGRLRDFPERLAKANYVIVNGKCSPSDQRELDLLNPAAKILVEPQISEI
CCCCCCEEECCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHCCCHHHEEECCCHHEE
VWLNKSANMPRDHWEGLGVGVFCGLGFPKGFLTMLRNAGIHVLGTHLLPDHSGITKQELE
EEECCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHH
LFCKKIILRQGVGILCTEKDSVKIEAFAEEMSLPIGEVRMRFSCVCNEERMVAMLDAIEA
HHHHHHHHHCCCCEEEECCCCEEHEEEHHHHCCCHHHHHEEEHEEECCCHHHHHHHHHHH
IQKNKKVTT
HHCCCCCCC
>Mature Secondary Structure
MRFSFLSGIRDLFRHFIISAASGALSDRLGWVWGAIAKVFSGSVWLRYKIAKPPHQVQAT
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHEE
VVSVGNIVVGGTGKTPLVLWLAQALNERGISCAVLSRGYKGKCSQRKSFTIVDPALHTAA
EEEECCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEECHHHHHHH
CVGDEPLLLAKHLPAGTVRIQKDRKALAEKSAGAFDVLLLDDGFQCNRLHKDVEIVLVNG
HCCCCCEEEEECCCCCEEEEEHHHHHHHHCCCCCEEEEEECCCCCHHCCCCCEEEEEEEC
SDPFGGRAFFPKGRLRDFPERLAKANYVIVNGKCSPSDQRELDLLNPAAKILVEPQISEI
CCCCCCEEECCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHCCCHHHEEECCCHHEE
VWLNKSANMPRDHWEGLGVGVFCGLGFPKGFLTMLRNAGIHVLGTHLLPDHSGITKQELE
EEECCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHH
LFCKKIILRQGVGILCTEKDSVKIEAFAEEMSLPIGEVRMRFSCVCNEERMVAMLDAIEA
HHHHHHHHHCCCCEEEECCCCEEHEEEHHHHCCCHHHHHEEEHEEECCCHHHHHHHHHHH
IQKNKKVTT
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935