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Identifier: 15835295

GI number: 15835295

Start: 812276

End: 813508

Strand: Reverse

Name: Not Available

Synonym: TC0680

Alternate gene names: 15835295

Gene position: 813508-812276 (Counterclockwise)

Preceding gene: 15835296

Following gene: 15835294

Centisome position: 75.82

GC content: 41.36

Gene sequence:

>1233_bases
TTGCCTTCACTTCATGTTGAGTTGAAAAACACTAAAACGTTTACTAGTTCAGAGATAAGGGGATTCATGTTTGAGTTTCG
ATTTCCAAAAATAGGGGAGACCGCATCTGGGGGTATAGTTGTCCGATGGTTGAAACAGGTCGGGGATTCTATTCAGAAAG
ATGAGCCATTGATCGAGGTCTCAACAGATAAGATTGCAACGGAGTTATCTCCTTCGCAAGCAGGTGTTTTAGAAGAGTGT
TTAGTTCAAGAAGGGGAGGAGGTTTCCCCTGGAGATGTTTTAGCGCGTTTGCGAGAAATTTCACCTGTAGATACATCGGT
ACCCACTTCCGTAGAAGAAAGTCCTATTAAGGAAGAATCTCTTGTAAACAGAGAGAATCAGTGGTTATCTCCCGCAGTTC
TAGGAATCGTGCAAAGAGAAGGACTAGATCTCCAAGAATTGCAAAAGATTTCTGGTACGGGAGAGAATAGTCGAATTACT
CGAAAAGATGTAGAACGTTATCTTTCTGATAAAAGAGAGGCTAGAGCTCCAATCTGTTCTAAGGAAGAAAATAGAATCCC
TATGTCTCCCTTGCGTCGGGCTATCGCTTCTTCTTTGCGACAGTCCTCGGAAGAGGTTCCGCATGCCTCTTTAGTTGTTG
ATGTGGATGTAACAGATTTAATGAATCTTATCTCTTTAGAACGGGAGCGTTTTGCGGCCGCTCATGGAGTTAAGCTTACA
ATTACAAGCTTCATTATACAATGCTTAGCCAAGTCTTTGGAACAATTTCCCTTGCTCAATGGATCTTTAGATGGAGATAC
GATTGTTTTAAAGAAAGCTGTGAATGTAGGTGTAGCTGTAAATTTAAATAAAGAAGGCGTGGTTGTGCCTGTCATTCATA
ATTGTCAGGATAGAGGATTAGTGAGTATCGCCAAAGCCCTTGCTGATCTCTCTTCTAGGGCTCGAGCTAGTAAGTTAGAT
GCTTCAGAAGCTAAAGGAGGGAGTGTCACCCTAACTAACTTTGGTATGACAGGAGCTCTTATTGGTATGCCAATTATCCG
TTATCCAGAAGTGGCGATCTTAGGAATAGGAACCATCCAAAAACGTGTTGTTGTGAGAGAGGATGATTCGTTGGCTATTA
GAAAAATGATGTATGTTACTTTGACATTTGATCATCGAGTTTTAGATGGGATTTACGGCGGAGAATTTTTAACCGCTTTG
AAAAATCGTTTAGAGTCTGTTACGATGAGCTAA

Upstream 100 bases:

>100_bases
TCTGTAGCTTCAAAAAGTGTTTAGTGTAACATGAGAAATATTTTAACGTCTTATGGGCCATTAGATCTTCAAGAGATCGT
TTCTTATCTGTAAAAAGATA

Downstream 100 bases:

>100_bases
AAAAGATTTTTAATAGGCGGGCGAATTCATGTACGTGCCTGATGTTGCAGAAGACCTTTGTTTAGATATTTTTCATAAGC
AAAAGCAGGTTATTTCGCGG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPSLHVELKNTKTFTSSEIRGFMFEFRFPKIGETASGGIVVRWLKQVGDSIQKDEPLIEVSTDKIATELSPSQAGVLEEC
LVQEGEEVSPGDVLARLREISPVDTSVPTSVEESPIKEESLVNRENQWLSPAVLGIVQREGLDLQELQKISGTGENSRIT
RKDVERYLSDKREARAPICSKEENRIPMSPLRRAIASSLRQSSEEVPHASLVVDVDVTDLMNLISLERERFAAAHGVKLT
ITSFIIQCLAKSLEQFPLLNGSLDGDTIVLKKAVNVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKALADLSSRARASKLD
ASEAKGGSVTLTNFGMTGALIGMPIIRYPEVAILGIGTIQKRVVVREDDSLAIRKMMYVTLTFDHRVLDGIYGGEFLTAL
KNRLESVTMS

Sequences:

>Translated_410_residues
MPSLHVELKNTKTFTSSEIRGFMFEFRFPKIGETASGGIVVRWLKQVGDSIQKDEPLIEVSTDKIATELSPSQAGVLEEC
LVQEGEEVSPGDVLARLREISPVDTSVPTSVEESPIKEESLVNRENQWLSPAVLGIVQREGLDLQELQKISGTGENSRIT
RKDVERYLSDKREARAPICSKEENRIPMSPLRRAIASSLRQSSEEVPHASLVVDVDVTDLMNLISLERERFAAAHGVKLT
ITSFIIQCLAKSLEQFPLLNGSLDGDTIVLKKAVNVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKALADLSSRARASKLD
ASEAKGGSVTLTNFGMTGALIGMPIIRYPEVAILGIGTIQKRVVVREDDSLAIRKMMYVTLTFDHRVLDGIYGGEFLTAL
KNRLESVTMS
>Mature_409_residues
PSLHVELKNTKTFTSSEIRGFMFEFRFPKIGETASGGIVVRWLKQVGDSIQKDEPLIEVSTDKIATELSPSQAGVLEECL
VQEGEEVSPGDVLARLREISPVDTSVPTSVEESPIKEESLVNRENQWLSPAVLGIVQREGLDLQELQKISGTGENSRITR
KDVERYLSDKREARAPICSKEENRIPMSPLRRAIASSLRQSSEEVPHASLVVDVDVTDLMNLISLERERFAAAHGVKLTI
TSFIIQCLAKSLEQFPLLNGSLDGDTIVLKKAVNVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKALADLSSRARASKLDA
SEAKGGSVTLTNFGMTGALIGMPIIRYPEVAILGIGTIQKRVVVREDDSLAIRKMMYVTLTFDHRVLDGIYGGEFLTALK
NRLESVTMS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=429, Percent_Identity=28.2051282051282, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI110671329, Length=425, Percent_Identity=24.7058823529412, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI203098753, Length=464, Percent_Identity=25.6465517241379, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=25.4424778761062, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=31.2775330396476, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=32.0512820512821, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786946, Length=396, Percent_Identity=31.3131313131313, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1786305, Length=411, Percent_Identity=28.4671532846715, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=29.2161520190024, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=429, Percent_Identity=26.8065268065268, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17537937, Length=440, Percent_Identity=25.4545454545455, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538894, Length=296, Percent_Identity=30.7432432432432, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=27.0731707317073, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=472, Percent_Identity=25, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24645909, Length=234, Percent_Identity=32.0512820512821, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=26.0765550239234, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=30.4932735426009, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129315, Length=223, Percent_Identity=30.4932735426009, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 44891; Mature: 44760

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLHVELKNTKTFTSSEIRGFMFEFRFPKIGETASGGIVVRWLKQVGDSIQKDEPLIEV
CCCEEEEECCCCCCCHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
STDKIATELSPSQAGVLEECLVQEGEEVSPGDVLARLREISPVDTSVPTSVEESPIKEES
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
LVNRENQWLSPAVLGIVQREGLDLQELQKISGTGENSRITRKDVERYLSDKREARAPICS
HHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
KEENRIPMSPLRRAIASSLRQSSEEVPHASLVVDVDVTDLMNLISLERERFAAAHGVKLT
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEE
ITSFIIQCLAKSLEQFPLLNGSLDGDTIVLKKAVNVGVAVNLNKEGVVVPVIHNCQDRGL
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCCCHH
VSIAKALADLSSRARASKLDASEAKGGSVTLTNFGMTGALIGMPIIRYPEVAILGIGTIQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCHHCCCCEEEEECCHHH
KRVVVREDDSLAIRKMMYVTLTFDHRVLDGIYGGEFLTALKNRLESVTMS
EEEEEECCCHHHEEEEEEEEEEECHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSLHVELKNTKTFTSSEIRGFMFEFRFPKIGETASGGIVVRWLKQVGDSIQKDEPLIEV
CCEEEEECCCCCCCHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
STDKIATELSPSQAGVLEECLVQEGEEVSPGDVLARLREISPVDTSVPTSVEESPIKEES
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
LVNRENQWLSPAVLGIVQREGLDLQELQKISGTGENSRITRKDVERYLSDKREARAPICS
HHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
KEENRIPMSPLRRAIASSLRQSSEEVPHASLVVDVDVTDLMNLISLERERFAAAHGVKLT
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEE
ITSFIIQCLAKSLEQFPLLNGSLDGDTIVLKKAVNVGVAVNLNKEGVVVPVIHNCQDRGL
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCCCHH
VSIAKALADLSSRARASKLDASEAKGGSVTLTNFGMTGALIGMPIIRYPEVAILGIGTIQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCHHCCCCEEEEECCHHH
KRVVVREDDSLAIRKMMYVTLTFDHRVLDGIYGGEFLTALKNRLESVTMS
EEEEEECCCHHHEEEEEEEEEEECHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]