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The map label for this gene is grpE

Identifier: 15835289

GI number: 15835289

Start: 804871

End: 805443

Strand: Direct

Name: grpE

Synonym: TC0674

Alternate gene names: 15835289

Gene position: 804871-805443 (Clockwise)

Preceding gene: 15835288

Following gene: 15835290

Centisome position: 75.01

GC content: 38.57

Gene sequence:

>573_bases
ATGACAGAAACCCCCAATACCTCGTCAGAAGAAATTCAGACTAGCGAGCCTTCGTCTGATAACGAACTCCAAACGCTTCA
ACAAGAGAACGCTAATTTAAAAGCCGAACTGAAAGAAAAAAATGACCGTTATCTTATGGCCCTTGCTGAAGCGGAAAATT
CAAGAAAACGTCTACAGAAAGAACGTACAGAAATGATGCAATATGCTGTAGAAAATGCTCTTTTGGATTTCCTCCCTCCA
ATGGAAAGTATGGAAAAAGCCTTGGGGTTCGCTTCTCAAACCTCTGATGAAGTGAAAAATTGGGCCATAGGGTTCCAAAT
GATCTTACAACAATTTAAACAAGTATTCGAAGATAAGGGTGTTGTCGAATACTCTTCAAAAGGAGAATTATTCAACCCTT
ATCTGCATGAAGCTGTAGAAATCGAAGAAACCACAGATATTCCGGAAGGGACTATCTTGGAGGAATTTACAAAAGGTTAT
AAGATAGGAGACCGTCCTATTCGTGTAGCTAAAGTGAAAGTAGCTAAATTTCCTACTAAAGGAAATAACGACAGTAACGA
AGAAAAAGAATAA

Upstream 100 bases:

>100_bases
CTTTCAGGAGACCTTGTCCGACCGACCCTAGATGTTCACAAAAACCAGCAGAGTTGACTCGACGTTCTTCTATAAAATTA
TTACCAGCTAAGGAATTAAC

Downstream 100 bases:

>100_bases
TTATCCTAGAAATTAGGTGTTCAATATGAGCGAAAAAAGAAAGTCTAACAAAATCATTGGTATCGACCTAGGGACGACTA
ACTCTTGCGTCTCTGTAATG

Product: grpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTETPNTSSEEIQTSEPSSDNELQTLQQENANLKAELKEKNDRYLMALAEAENSRKRLQKERTEMMQYAVENALLDFLPP
MESMEKALGFASQTSDEVKNWAIGFQMILQQFKQVFEDKGVVEYSSKGELFNPYLHEAVEIEETTDIPEGTILEEFTKGY
KIGDRPIRVAKVKVAKFPTKGNNDSNEEKE

Sequences:

>Translated_190_residues
MTETPNTSSEEIQTSEPSSDNELQTLQQENANLKAELKEKNDRYLMALAEAENSRKRLQKERTEMMQYAVENALLDFLPP
MESMEKALGFASQTSDEVKNWAIGFQMILQQFKQVFEDKGVVEYSSKGELFNPYLHEAVEIEETTDIPEGTILEEFTKGY
KIGDRPIRVAKVKVAKFPTKGNNDSNEEKE
>Mature_189_residues
TETPNTSSEEIQTSEPSSDNELQTLQQENANLKAELKEKNDRYLMALAEAENSRKRLQKERTEMMQYAVENALLDFLPPM
ESMEKALGFASQTSDEVKNWAIGFQMILQQFKQVFEDKGVVEYSSKGELFNPYLHEAVEIEETTDIPEGTILEEFTKGYK
IGDRPIRVAKVKVAKFPTKGNNDSNEEKE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=158, Percent_Identity=31.6455696202532, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788967, Length=172, Percent_Identity=29.6511627906977, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CHLMU (P23575)

Other databases:

- EMBL:   M62819
- EMBL:   AE002160
- PIR:   G81676
- RefSeq:   NP_297048.1
- ProteinModelPortal:   P23575
- SMR:   P23575
- GeneID:   1246035
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0674
- TIGR:   TC_0674
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- ProtClustDB:   CLSK871354
- BioCyc:   CMUR243161:TC_0674-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21723; Mature: 21592

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETPNTSSEEIQTSEPSSDNELQTLQQENANLKAELKEKNDRYLMALAEAENSRKRLQK
CCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCEEHHCCCCCEEEEEHHHHHHHHHHHHH
ERTEMMQYAVENALLDFLPPMESMEKALGFASQTSDEVKNWAIGFQMILQQFKQVFEDKG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
VVEYSSKGELFNPYLHEAVEIEETTDIPEGTILEEFTKGYKIGDRPIRVAKVKVAKFPTK
CEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEEHEECCCC
GNNDSNEEKE
CCCCCCCCCC
>Mature Secondary Structure 
TETPNTSSEEIQTSEPSSDNELQTLQQENANLKAELKEKNDRYLMALAEAENSRKRLQK
CCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCEEHHCCCCCEEEEEHHHHHHHHHHHHH
ERTEMMQYAVENALLDFLPPMESMEKALGFASQTSDEVKNWAIGFQMILQQFKQVFEDKG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
VVEYSSKGELFNPYLHEAVEIEETTDIPEGTILEEFTKGYKIGDRPIRVAKVKVAKFPTK
CEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEEHEECCCC
GNNDSNEEKE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254267; 10684935