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The map label for this gene is proS

Identifier: 15835287

GI number: 15835287

Start: 801883

End: 803622

Strand: Direct

Name: proS

Synonym: TC0672

Alternate gene names: 15835287

Gene position: 801883-803622 (Clockwise)

Preceding gene: 15835285

Following gene: 15835288

Centisome position: 74.74

GC content: 42.24

Gene sequence:

>1740_bases
ATGAAAACATCCCTCTTGTTTTATAGAACCTCTAAAAACGCTAATAAAGAAGCCTCTGTTCTTTCTTACGAACTTCTTGA
AAAGGCCGGCTATCTTTTTAAAACATCTAAAGGGATTTACTCATATACTCCTCTTTTCCAAAGAGTTATCTTGAAAATGA
CGGAGATTATTCGAGAGGAACTCAATGCGATTGGCGGACAAGAAGTCTGTCTTCCCCTTCTGCAATCTGCAGAATTATGG
CAAAAAACAGGTCGCTGGGAAGCCTTTCTATCAGAAAAACTCCTCTATGTTCTTAAAGACAGAGAAAACAAGGAGATGTG
CTTAGCTCCCACCCATGAAGAAGTGGTCTCAGAGTTCGTGTCACAATGGCTGACAGGAAGAAAGCAACTCCCTGTCCACC
TATATCAAATTGGCACCAAATTCCGGGATGAAATTCGACCTCGTTTTGGGCTCATGCGAGCAAAAGAATTTCTAATGGAG
GATAGCTATACTTTCTCAGACTCTCCAGAACAGATGGAAGAGCAGTATGCAAAACTTCGTTTGGCATATCAACGCATCTT
TGATCGATTGAACCTCAAGTATGTCATTGTTACTGCTGATGGAGGAAAAATCGGCAAAGGTAAATCTGAAGAATTTCATG
TCCTCTGCTCGCTAGGAGAGGACACGATCTGTGTTAGTGGCTCTTACGGAGCTAATATAGAAGCTGCCCAGGCTATCCCC
CCATCCTACACTTACTCCTCTGAACTCCTTCCTATGAAAGAGGTCGCCACACCCAATGTTCGTACTATTGAAGACTTAGA
GACCTTTTTCAACACCCCTAGACAGCAAATCATTAAAACCTTAGTTGTAAAAGTTCACAAAAAAGGCAGCGAACAGTTCT
TTGCTATTTGCATTCGCGGGGATCGTCAGGTTAACTTAACTAAAGTGTCCTCTTTCCTACAAGCTGACGACTGCGAACTC
GCTTCCGATGAAGAGATTATTAAACATCTTCATGTTGAAAAGGGATTCATTGGACCGCTACACTGTCCCGTTCCCTGCTA
CGCTGATGAAACCACTCGCCCTATGACGAACTTTATCTGCGCAAATAATCAAAAAGATATTCACTGTAGATACGTAAACT
GGGAAAGAGACATTCCTCTTCCTATTTTTGGAGATTTTCTTCTAGCCGAATCAGGAGACCTTTGCCCTCAAAATAATGAA
GCTCCCTATGAAATTTTCCAGGGTGTCGAAGTGGCTCATATTTTTAATCTTGGGACACGATATACGGAATGTTTCTCTGT
AGGATTCCAAGATGAGAATGGGGAGAAGCAGCTCTGTTGGATGGGCACATATGGAATCGGAGTAGGAAGGACTCTTGCTG
CTTGCATAGAACAACTTGCCGATAGCAAGGGGATCGTTTGGCCATTAGCCGTTGCTCCATTCTCTATTACAATCCTCTAT
AACGGGGGCGATACCGAAGGAGAGGCTGTTGCTTCACAATTTTATCAAAGCCTAAACACTGCGGGATTCGAGCCATTACT
TGACGATCGTAATGAGCGCCTTGGCTTTAAATTGAAAGATAGCGATCTACTTGGAATTCCCTATAAATTGATCATTGGGA
AGTCTTTCCAAAAGACTGGCATGTTAGAGATCGAATCTCGATCTGGAGAAAAATATAACATCTCTCCTGAAAATCTGTTA
GATTGGTGCTCTAAAAATCTTCCTTGTCATACAAGAAAAATCCCTCCTATTCGAGAGTAA

Upstream 100 bases:

>100_bases
ATTAAATCCAGCATGAACGCAAATTACAGAGATTGATCCAGAAACAATCCTGTGCTAAAATTCCCTTCCTTGCGAAAAAA
TACAAAGCGCTTACTTCCTT

Downstream 100 bases:

>100_bases
ACTAGCACTCTTTGCTCGCGAGCGCTAAAATTCTTGACCAGAGGCTCCGGTTTTCCTATAATGACACCGACTTATGGAAA
ATAGAACAGAAATGTCCCAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MKTSLLFYRTSKNANKEASVLSYELLEKAGYLFKTSKGIYSYTPLFQRVILKMTEIIREELNAIGGQEVCLPLLQSAELW
QKTGRWEAFLSEKLLYVLKDRENKEMCLAPTHEEVVSEFVSQWLTGRKQLPVHLYQIGTKFRDEIRPRFGLMRAKEFLME
DSYTFSDSPEQMEEQYAKLRLAYQRIFDRLNLKYVIVTADGGKIGKGKSEEFHVLCSLGEDTICVSGSYGANIEAAQAIP
PSYTYSSELLPMKEVATPNVRTIEDLETFFNTPRQQIIKTLVVKVHKKGSEQFFAICIRGDRQVNLTKVSSFLQADDCEL
ASDEEIIKHLHVEKGFIGPLHCPVPCYADETTRPMTNFICANNQKDIHCRYVNWERDIPLPIFGDFLLAESGDLCPQNNE
APYEIFQGVEVAHIFNLGTRYTECFSVGFQDENGEKQLCWMGTYGIGVGRTLAACIEQLADSKGIVWPLAVAPFSITILY
NGGDTEGEAVASQFYQSLNTAGFEPLLDDRNERLGFKLKDSDLLGIPYKLIIGKSFQKTGMLEIESRSGEKYNISPENLL
DWCSKNLPCHTRKIPPIRE

Sequences:

>Translated_579_residues
MKTSLLFYRTSKNANKEASVLSYELLEKAGYLFKTSKGIYSYTPLFQRVILKMTEIIREELNAIGGQEVCLPLLQSAELW
QKTGRWEAFLSEKLLYVLKDRENKEMCLAPTHEEVVSEFVSQWLTGRKQLPVHLYQIGTKFRDEIRPRFGLMRAKEFLME
DSYTFSDSPEQMEEQYAKLRLAYQRIFDRLNLKYVIVTADGGKIGKGKSEEFHVLCSLGEDTICVSGSYGANIEAAQAIP
PSYTYSSELLPMKEVATPNVRTIEDLETFFNTPRQQIIKTLVVKVHKKGSEQFFAICIRGDRQVNLTKVSSFLQADDCEL
ASDEEIIKHLHVEKGFIGPLHCPVPCYADETTRPMTNFICANNQKDIHCRYVNWERDIPLPIFGDFLLAESGDLCPQNNE
APYEIFQGVEVAHIFNLGTRYTECFSVGFQDENGEKQLCWMGTYGIGVGRTLAACIEQLADSKGIVWPLAVAPFSITILY
NGGDTEGEAVASQFYQSLNTAGFEPLLDDRNERLGFKLKDSDLLGIPYKLIIGKSFQKTGMLEIESRSGEKYNISPENLL
DWCSKNLPCHTRKIPPIRE
>Mature_579_residues
MKTSLLFYRTSKNANKEASVLSYELLEKAGYLFKTSKGIYSYTPLFQRVILKMTEIIREELNAIGGQEVCLPLLQSAELW
QKTGRWEAFLSEKLLYVLKDRENKEMCLAPTHEEVVSEFVSQWLTGRKQLPVHLYQIGTKFRDEIRPRFGLMRAKEFLME
DSYTFSDSPEQMEEQYAKLRLAYQRIFDRLNLKYVIVTADGGKIGKGKSEEFHVLCSLGEDTICVSGSYGANIEAAQAIP
PSYTYSSELLPMKEVATPNVRTIEDLETFFNTPRQQIIKTLVVKVHKKGSEQFFAICIRGDRQVNLTKVSSFLQADDCEL
ASDEEIIKHLHVEKGFIGPLHCPVPCYADETTRPMTNFICANNQKDIHCRYVNWERDIPLPIFGDFLLAESGDLCPQNNE
APYEIFQGVEVAHIFNLGTRYTECFSVGFQDENGEKQLCWMGTYGIGVGRTLAACIEQLADSKGIVWPLAVAPFSITILY
NGGDTEGEAVASQFYQSLNTAGFEPLLDDRNERLGFKLKDSDLLGIPYKLIIGKSFQKTGMLEIESRSGEKYNISPENLL
DWCSKNLPCHTRKIPPIRE

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=43.3628318584071, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=39.6160558464223, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI115532348, Length=217, Percent_Identity=39.63133640553, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=98, Percent_Identity=34.6938775510204, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=564, Percent_Identity=28.0141843971631, Blast_Score=217, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24656200, Length=267, Percent_Identity=37.8277153558052, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 65781; Mature: 65781

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSLLFYRTSKNANKEASVLSYELLEKAGYLFKTSKGIYSYTPLFQRVILKMTEIIREE
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LNAIGGQEVCLPLLQSAELWQKTGRWEAFLSEKLLYVLKDRENKEMCLAPTHEEVVSEFV
HHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCEEECCCHHHHHHHHH
SQWLTGRKQLPVHLYQIGTKFRDEIRPRFGLMRAKEFLMEDSYTFSDSPEQMEEQYAKLR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LAYQRIFDRLNLKYVIVTADGGKIGKGKSEEFHVLCSLGEDTICVSGSYGANIEAAQAIP
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHCCCC
PSYTYSSELLPMKEVATPNVRTIEDLETFFNTPRQQIIKTLVVKVHKKGSEQFFAICIRG
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEC
DRQVNLTKVSSFLQADDCELASDEEIIKHLHVEKGFIGPLHCPVPCYADETTRPMTNFIC
CCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHEE
ANNQKDIHCRYVNWERDIPLPIFGDFLLAESGDLCPQNNEAPYEIFQGVEVAHIFNLGTR
CCCCCCEEEEEECCCCCCCCCHHCCHHEECCCCCCCCCCCCHHHHHCCCCEEHHHHCCCH
YTECFSVGFQDENGEKQLCWMGTYGIGVGRTLAACIEQLADSKGIVWPLAVAPFSITILY
HHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEE
NGGDTEGEAVASQFYQSLNTAGFEPLLDDRNERLGFKLKDSDLLGIPYKLIIGKSFQKTG
CCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCC
MLEIESRSGEKYNISPENLLDWCSKNLPCHTRKIPPIRE
CEEEECCCCCEECCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTSLLFYRTSKNANKEASVLSYELLEKAGYLFKTSKGIYSYTPLFQRVILKMTEIIREE
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LNAIGGQEVCLPLLQSAELWQKTGRWEAFLSEKLLYVLKDRENKEMCLAPTHEEVVSEFV
HHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCEEECCCHHHHHHHHH
SQWLTGRKQLPVHLYQIGTKFRDEIRPRFGLMRAKEFLMEDSYTFSDSPEQMEEQYAKLR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LAYQRIFDRLNLKYVIVTADGGKIGKGKSEEFHVLCSLGEDTICVSGSYGANIEAAQAIP
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHCCCC
PSYTYSSELLPMKEVATPNVRTIEDLETFFNTPRQQIIKTLVVKVHKKGSEQFFAICIRG
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEC
DRQVNLTKVSSFLQADDCELASDEEIIKHLHVEKGFIGPLHCPVPCYADETTRPMTNFIC
CCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHEE
ANNQKDIHCRYVNWERDIPLPIFGDFLLAESGDLCPQNNEAPYEIFQGVEVAHIFNLGTR
CCCCCCEEEEEECCCCCCCCCHHCCHHEECCCCCCCCCCCCHHHHHCCCCEEHHHHCCCH
YTECFSVGFQDENGEKQLCWMGTYGIGVGRTLAACIEQLADSKGIVWPLAVAPFSITILY
HHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEE
NGGDTEGEAVASQFYQSLNTAGFEPLLDDRNERLGFKLKDSDLLGIPYKLIIGKSFQKTG
CCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCC
MLEIESRSGEKYNISPENLLDWCSKNLPCHTRKIPPIRE
CEEEECCCCCEECCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA