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The map label for this gene is ligA

Identifier: 15835041

GI number: 15835041

Start: 484203

End: 486185

Strand: Direct

Name: ligA

Synonym: TC0423

Alternate gene names: 15835041

Gene position: 484203-486185 (Clockwise)

Preceding gene: 15835040

Following gene: 15835042

Centisome position: 45.13

GC content: 41.65

Gene sequence:

>1983_bases
ATGAGAGCTTTGTCTCAAGACTATTATATTGCTTTATGTACAGAGTTGATAGAGCATGATCGGCGTTATTATGTGTTGGA
TCGACCTACAATTTCTGATTACAGTTATGATATGAAGATGCGAGAGCTTCAAAAAATAGAAGCTCAACATCCGGAGTGGA
AGGTGCCTTGGTCGCCAACCATTTATTTGGGAGATCGTCCTTCTGGTCAGTTTCCTGTAGTTCCTCATTCACATCCTATG
GTGTCTATAGCGAATGTGTACTCTTTAGAGGAATTAGAGGAATTTTTTTCCCGGACAGAAAAGCTGCTTGGTTATTTCCC
TACATATTCTTTAGAACTCAAAATTGATGGAATCGCTGTTGCGATTCGTTATGAAAACAGGACGTTTGCTCAGGCTTTGA
GCAGAGGAAATGGAGTTAAAGGGGAAGATATTACAGCTAACGTAAGTACGATACGTTCTTTGCCTATGACTCTTCCTCAA
GATGCACCAGAAGAGGTAGAGGTTCGAGGAGAGGTATTTCTTTCATACCAGGCTTTTGAGAAGCTCAATATTTGTCAGCG
AGAACAAGGGAAATTAGAATTTGCGAATCCTCGCAATGCTGCTGGAGGGACTCTTAAGCTGTTGTCTTCTAGGGAGGCAG
CTAAAAGGAATCTGGACTTATCCATTTATGGACTCATCACGAATCGTGAAGAGCATTCTCACTTTGAAAATCTTGGTCTA
TGTGCTCATTGGGGATTTCCTATCGCAGGCATGCCTAAACAGTGCCGCACGAAAGAAGAGGTTATAGAACGCATACGAGA
AGTTGAAGGAATGCGATCGCGGCTTCCCATGGCCATAGATGGGGTGGTCGTTAAGGTCGATAGTGTTGCCGAGCAGCATC
GTTTAGGATTGACTAGCAAGCATTATCGTTGGGCGATAGCCTACAAATATGCTCCGGAAAGAGCAGAAACCATTTTACAA
GATATTACCGTGCAGGTTGGTAAAACAGGGATTTTAACTCCAGTTGCCGAACTAACTCCCGTTTTGCTGTCTGGGAGCCG
AGTGTCTAGAGCATCTCTATACAACGAGGATGAAATAGAGAAGAAGGACATTCGCATAGGAGATTCTGTTTACGTAGAAA
AAGGTGGAGAAATTATCCCTAAAATCGTTGGGATTAATTTAGCTAAGCGTTCTCCTGATAGTAAACCTTGGAAAATGCCA
AGCCTATGTCCTATGTGCCATAATCCAGTCATTAAAGAAAGAGTGTCTGTTCGTTGTACAAACCCTCTTTGCCTTGGGGG
AATGTTAGAAAAGATTTGTTTCTTTGCAAGTAAGGGAGCTTTGAATATTGATCATTTAGGCGAGAAGGTTATAGCCAAAT
TATTTGAAATGGGGCTGATTCGTTCTTGTTCCGATGTGTTTGCGCTTACAGAAAATGATTTGAAGCAGGTTCCAGGATTT
AAGGACCGTTCTGTCCACAATTTATTAGCGAGTATTGCAGGAGCCAAAGAAGTGACTTTGGATCGATTTTTGACAGCGCT
TTCCATACCTTTTGTAGGGAGTTCTGGGGCTATAGCTCTAGCGGACCATTTTGGAACTTTAGATAAAGTAATGAAAGCTT
CCTTGGATGAATTACTCTCTATAGAAGGTATTGGTCAAAAAGTTGCAGCGTCTATCATAGATTTCTTTTCTAAAACTGAG
AATGTAGAAGAGATTTGTCGTATGCAAGAGTTGGGGGTTCGCATTCTTCCTAAACAGGTAGATAGATGGGCCCCTTTACA
GGGTAAGATTTTTGTTCTTACAGGGACTCTTCAGGAGATGACGCGATTACAAGCCGAGGAGCGTATTCGTTCGTTAGGAG
GAAAGATTAGTTCTTCTGTATCGAAGAATACCTATGCTGTAGTTGTGGGAAGAGAAGCTGGGTCTAAGTTAAAAAAAGCT
CAGGAATTAGGGGTGCAGGTTCTAGAAGAAAGTGATTTGTTAAAAATTCTTAATCAAAACTAG

Upstream 100 bases:

>100_bases
GAGGATCTCCGTTGTGCACATCGCCACCGAAACAATCCAGGGGCAATTAATAGCACCTATGGGTTTCGTTGTGCGAAAGA
TGTAAAATAAAGGGCTTGCT

Downstream 100 bases:

>100_bases
CTAATTTCAAGATGCGTTTTACAAAGCTTTTATCAAGAGATCTGAGATTTATATGTAAGAATTTGGTATAATATTTTTCT
TTAAAAAAGATTTTTTAGGA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MRALSQDYYIALCTELIEHDRRYYVLDRPTISDYSYDMKMRELQKIEAQHPEWKVPWSPTIYLGDRPSGQFPVVPHSHPM
VSIANVYSLEELEEFFSRTEKLLGYFPTYSLELKIDGIAVAIRYENRTFAQALSRGNGVKGEDITANVSTIRSLPMTLPQ
DAPEEVEVRGEVFLSYQAFEKLNICQREQGKLEFANPRNAAGGTLKLLSSREAAKRNLDLSIYGLITNREEHSHFENLGL
CAHWGFPIAGMPKQCRTKEEVIERIREVEGMRSRLPMAIDGVVVKVDSVAEQHRLGLTSKHYRWAIAYKYAPERAETILQ
DITVQVGKTGILTPVAELTPVLLSGSRVSRASLYNEDEIEKKDIRIGDSVYVEKGGEIIPKIVGINLAKRSPDSKPWKMP
SLCPMCHNPVIKERVSVRCTNPLCLGGMLEKICFFASKGALNIDHLGEKVIAKLFEMGLIRSCSDVFALTENDLKQVPGF
KDRSVHNLLASIAGAKEVTLDRFLTALSIPFVGSSGAIALADHFGTLDKVMKASLDELLSIEGIGQKVAASIIDFFSKTE
NVEEICRMQELGVRILPKQVDRWAPLQGKIFVLTGTLQEMTRLQAEERIRSLGGKISSSVSKNTYAVVVGREAGSKLKKA
QELGVQVLEESDLLKILNQN

Sequences:

>Translated_660_residues
MRALSQDYYIALCTELIEHDRRYYVLDRPTISDYSYDMKMRELQKIEAQHPEWKVPWSPTIYLGDRPSGQFPVVPHSHPM
VSIANVYSLEELEEFFSRTEKLLGYFPTYSLELKIDGIAVAIRYENRTFAQALSRGNGVKGEDITANVSTIRSLPMTLPQ
DAPEEVEVRGEVFLSYQAFEKLNICQREQGKLEFANPRNAAGGTLKLLSSREAAKRNLDLSIYGLITNREEHSHFENLGL
CAHWGFPIAGMPKQCRTKEEVIERIREVEGMRSRLPMAIDGVVVKVDSVAEQHRLGLTSKHYRWAIAYKYAPERAETILQ
DITVQVGKTGILTPVAELTPVLLSGSRVSRASLYNEDEIEKKDIRIGDSVYVEKGGEIIPKIVGINLAKRSPDSKPWKMP
SLCPMCHNPVIKERVSVRCTNPLCLGGMLEKICFFASKGALNIDHLGEKVIAKLFEMGLIRSCSDVFALTENDLKQVPGF
KDRSVHNLLASIAGAKEVTLDRFLTALSIPFVGSSGAIALADHFGTLDKVMKASLDELLSIEGIGQKVAASIIDFFSKTE
NVEEICRMQELGVRILPKQVDRWAPLQGKIFVLTGTLQEMTRLQAEERIRSLGGKISSSVSKNTYAVVVGREAGSKLKKA
QELGVQVLEESDLLKILNQN
>Mature_660_residues
MRALSQDYYIALCTELIEHDRRYYVLDRPTISDYSYDMKMRELQKIEAQHPEWKVPWSPTIYLGDRPSGQFPVVPHSHPM
VSIANVYSLEELEEFFSRTEKLLGYFPTYSLELKIDGIAVAIRYENRTFAQALSRGNGVKGEDITANVSTIRSLPMTLPQ
DAPEEVEVRGEVFLSYQAFEKLNICQREQGKLEFANPRNAAGGTLKLLSSREAAKRNLDLSIYGLITNREEHSHFENLGL
CAHWGFPIAGMPKQCRTKEEVIERIREVEGMRSRLPMAIDGVVVKVDSVAEQHRLGLTSKHYRWAIAYKYAPERAETILQ
DITVQVGKTGILTPVAELTPVLLSGSRVSRASLYNEDEIEKKDIRIGDSVYVEKGGEIIPKIVGINLAKRSPDSKPWKMP
SLCPMCHNPVIKERVSVRCTNPLCLGGMLEKICFFASKGALNIDHLGEKVIAKLFEMGLIRSCSDVFALTENDLKQVPGF
KDRSVHNLLASIAGAKEVTLDRFLTALSIPFVGSSGAIALADHFGTLDKVMKASLDELLSIEGIGQKVAASIIDFFSKTE
NVEEICRMQELGVRILPKQVDRWAPLQGKIFVLTGTLQEMTRLQAEERIRSLGGKISSSVSKNTYAVVVGREAGSKLKKA
QELGVQVLEESDLLKILNQN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=663, Percent_Identity=40.1206636500754, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI87082305, Length=474, Percent_Identity=22.9957805907173, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CHLMU (Q9PKP2)

Other databases:

- EMBL:   AE002160
- PIR:   A81704
- RefSeq:   NP_296800.1
- ProteinModelPortal:   Q9PKP2
- SMR:   Q9PKP2
- GeneID:   1245776
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0423
- TIGR:   TC_0423
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q9PKP2
- ProtClustDB:   PRK07956
- BioCyc:   CMUR243161:TC_0423-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73786; Mature: 73786

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 114-114 BINDING 112-112 BINDING 135-135 BINDING 171-171 BINDING 285-285 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALSQDYYIALCTELIEHDRRYYVLDRPTISDYSYDMKMRELQKIEAQHPEWKVPWSPT
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCE
IYLGDRPSGQFPVVPHSHPMVSIANVYSLEELEEFFSRTEKLLGYFPTYSLELKIDGIAV
EEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEE
AIRYENRTFAQALSRGNGVKGEDITANVSTIRSLPMTLPQDAPEEVEVRGEVFLSYQAFE
EEEECCHHHHHHHHCCCCCCCCCCEECHHHHHHCCCCCCCCCCHHHEECCCEEEEHHHHH
KLNICQREQGKLEFANPRNAAGGTLKLLSSREAAKRNLDLSIYGLITNREEHSHFENLGL
HHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCE
CAHWGFPIAGMPKQCRTKEEVIERIREVEGMRSRLPMAIDGVVVKVDSVAEQHRLGLTSK
EEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCEEECCEEEEEHHHHHHHHCCCCCC
HYRWAIAYKYAPERAETILQDITVQVGKTGILTPVAELTPVLLSGSRVSRASLYNEDEIE
CEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCC
KKDIRIGDSVYVEKGGEIIPKIVGINLAKRSPDSKPWKMPSLCPMCHNPVIKERVSVRCT
HHHEECCCEEEEECCCCCCHHHHCCEECCCCCCCCCCCCCCCCCHHCCCHHHCCCCEEEC
NPLCLGGMLEKICFFASKGALNIDHLGEKVIAKLFEMGLIRSCSDVFALTENDLKQVPGF
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
KDRSVHNLLASIAGAKEVTLDRFLTALSIPFVGSSGAIALADHFGTLDKVMKASLDELLS
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHH
IEGIGQKVAASIIDFFSKTENVEEICRMQELGVRILPKQVDRWAPLQGKIFVLTGTLQEM
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCHHHHCCCCCCCEEEEEECCHHHH
TRLQAEERIRSLGGKISSSVSKNTYAVVVGREAGSKLKKAQELGVQVLEESDLLKILNQN
HHHHHHHHHHHHCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MRALSQDYYIALCTELIEHDRRYYVLDRPTISDYSYDMKMRELQKIEAQHPEWKVPWSPT
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCE
IYLGDRPSGQFPVVPHSHPMVSIANVYSLEELEEFFSRTEKLLGYFPTYSLELKIDGIAV
EEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEE
AIRYENRTFAQALSRGNGVKGEDITANVSTIRSLPMTLPQDAPEEVEVRGEVFLSYQAFE
EEEECCHHHHHHHHCCCCCCCCCCEECHHHHHHCCCCCCCCCCHHHEECCCEEEEHHHHH
KLNICQREQGKLEFANPRNAAGGTLKLLSSREAAKRNLDLSIYGLITNREEHSHFENLGL
HHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCCE
CAHWGFPIAGMPKQCRTKEEVIERIREVEGMRSRLPMAIDGVVVKVDSVAEQHRLGLTSK
EEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCEEECCEEEEEHHHHHHHHCCCCCC
HYRWAIAYKYAPERAETILQDITVQVGKTGILTPVAELTPVLLSGSRVSRASLYNEDEIE
CEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCC
KKDIRIGDSVYVEKGGEIIPKIVGINLAKRSPDSKPWKMPSLCPMCHNPVIKERVSVRCT
HHHEECCCEEEEECCCCCCHHHHCCEECCCCCCCCCCCCCCCCCHHCCCHHHCCCCEEEC
NPLCLGGMLEKICFFASKGALNIDHLGEKVIAKLFEMGLIRSCSDVFALTENDLKQVPGF
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
KDRSVHNLLASIAGAKEVTLDRFLTALSIPFVGSSGAIALADHFGTLDKVMKASLDELLS
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHH
IEGIGQKVAASIIDFFSKTENVEEICRMQELGVRILPKQVDRWAPLQGKIFVLTGTLQEM
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCHHHHCCCCCCCEEEEEECCHHHH
TRLQAEERIRSLGGKISSSVSKNTYAVVVGREAGSKLKKAQELGVQVLEESDLLKILNQN
HHHHHHHHHHHHCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935