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The map label for this gene is clpB

Identifier: 15835008

GI number: 15835008

Start: 451433

End: 454036

Strand: Direct

Name: clpB

Synonym: TC0389

Alternate gene names: 15835008

Gene position: 451433-454036 (Clockwise)

Preceding gene: 15835003

Following gene: 15835009

Centisome position: 42.07

GC content: 41.13

Gene sequence:

>2604_bases
ATGGAGAAGTTTTCAGACGCGGTCAGCGAGGCCTTAGAAAAGGCTTTTGAGTTAGCCAAAGATGCTAAGCATTCTTATGT
AACAGAAAATCATTTGCTAAAGAGTCTTTTGCAAAACCCAGGTTCTTTGTTCTGTTTAGTTATCAAGGATGTGCATGGGA
ATCTAGGACTATTAACTTCTGCTGTGGATGACGCTTTGCATAGAGAGCCAACTGTGGTTGAGGGTGCAGCTATTCCCAAA
CCATCGCCAAGTTTACAGCAATTATTGCTAAACGCTCAAGAAGAAGCTCGAAGTATGGGGGATGAATATCTCTCTGGAGA
TCACTTGTTATTAGCTTTTTGGAAAACTACCAAAGAGCCTTTTGCTTCATGGAGAAAAACAGTAAAAACTTCTCCGGAAG
CTTTGAAAGAATTAATCATCAAATTAAGACAAGGAAGTCGTATGGACTCGCCCAGTGCCGAAGAAAATCTAAAAGGGTTA
GAGAAATACTGTAAAAATCTGACTATTCTTGCAAGAGAAGGCAAATTGGATCCTGTGATCGGTCGAGATGAAGAGATTAG
GCGTACGATACAGGTCCTTTCTAGACGAACCAAGAATAATCCCATGTTGATAGGAGAGCCTGGGGTTGGAAAGACTGCGA
TTGCCGAAGGACTTGCTCTTCGGATTGTGCAAGGAGATGTTCCAGAAAGTTTAAAGGATAAGCATCTCTACGTGCTAGAT
ATGGGAGCGCTGATTGCTGGTGCGAAATATCGAGGAGAATTTGAAGAGCGTTTGAAAAGCGTTCTGAAAGGAGTAGAAGC
TTCGGAAGGAGCATGCATTCTATTTATCGACGAGGTACATACCTTAGTTGGGGCAGGAGCCACAGATGGGGCTATGGATG
CTGCAAATTTATTAAAGCCAGCTTTAGCTCGAGGAACGTTGCACTGTATTGGGGCAACGACTTTGAATGAATATCAGAAG
TACATCGAAAAGGATGCTGCTTTAGAAAGACGTTTCCAACCTATTTTTGTCACCGAGCCCTCCTTGGAAGATGCCGTTTT
TATTCTTCGAGGATTACGAGAAAAGTACGAAATTTTTCATGGGGTACGCATTACAGAAGGAGCTTTGAATGCTGCTGTAG
TTCTTTCTTATCGTTATATTACGGATCGTTTTCTTCCAGACAAGGCGATTGATTTGATCGATGAGGCTGCTAGTTTGATT
CGTATGCAGATAGGAAGTTTACCTTTGCCTATCGATGAAAAAGAGCGGGAATTGTCAGCTTTGATTGTTAAGCAAGAGGC
TATTAAGCGGGAGCAGGCTCCTGCTTATCAGGAAGAAGCCAATGAAATGCAAAAAGCTATCGATCGCGTTAAAGAAGAGC
TGGCGGCTTTACGGTTGCGCTGGGATGAAGAAAAAGGGTTAATTGCGGGATTAAAAGAGAAAAAAAATTCTTTAGAAAAT
TTAAAATTTGCAGAAGAAGAAGCTGAGAGGACCGCGGACTACAACAGAGTAGCGGAACTTCGCTATAGTCTCATTCCTTC
ACTTGAAAAAGAAATTCGTTTGGCGGAAGAAGCTTTGAATCAGAGAGATGGGCGTCTTTTGCAGGAAGAGGTGGATGAGC
GGTTAATTGCTCAAGTTGTTGCCAATTGGACTGGGATCCCTGTGCAAAAAATGTTAGAAGGAGAGTCTGAGAAGTTATTA
GTCTTGGAAGAATCTTTGGAAGAACGGGTGGTAGGACAGCCGTTTGCGATTACTGCTGTTAGTGATTCTATTCGATCTGC
TCGAGTAGGTTTAAGCGATCCACAGCGTCCACTAGGTGTGTTTCTCTTTTTAGGGCCTACAGGAGTCGGGAAAACGGAAC
TTGCTAAGGCCTTAGCAGAGCTTTTATTCAATAAAGAAGAAGCTATGATTCGGTTTGATATGACCGAATATATGGAAAAA
CATTCTGTTTCAAAATTAATAGGCTCTCCTCCAGGATATGTAGGTTACGAAGAGGGGGGTAGCCTTTCCGAAGCCTTAAG
AAGGCGTCCTTATTCTGTAGTTCTTTTTGATGAGATAGAGAAAGCTGACAAAGAAGTATTTAATATTTTGTTACAAATTT
TTGATGACGGAATTCTTACTGATAGCAAGAAACGTAAAGTAAACTGTAAGAATGCTCTTTTCATTATGACATCTAATATT
GGGTCACAAGAATTGGCGGATTATTGTGCGAAGAAGGGGACTATAGTCGATAAAGAGGCTGTGTTATCTGTCGTTGCTCC
TGCACTAAAAAATTATTTTAGTCCGGAATTTATCAACCGGATAGATGAAATCCTTCCATTTGTTCCTTTGACTACAGAAG
ATATTGTCAAAATCGTAGGCATTCAAATGAATCGAGTGGCTTTGCGTTTGTTAGAAAGAAGAATTTCATTAACTTGGGAT
GATTCTGTGGTTCTATTTCTTAGCGAGCAAGGTTATGATGGTGCTTTTGGGGCTCGTCCTTTGAAACGTTTGATTCAACA
AAAAGTGGTGACTATGTTGTCTAAGGCTCTTTTGAAGGGGGATATTAAATCCGGCATGTCAGTAGAGCTTACTATGGCCA
AGGATGTTGTTGTTTTTAAAACTAAAACAAATTCTGTGGTGTAG

Upstream 100 bases:

>100_bases
TGTATGGATATGAGGTCTGTTTTATAGATCTAAGTGGTCGATAGGTCTCTTCTTATGAAAATAAGTGGTTTTCTGCAGAA
GTTATGATAAAAAGGTGTTT

Downstream 100 bases:

>100_bases
GGCGTTTGTGTGTGGAAGCACTGGATTCGCATTATTGGGATAGGTAGTCTTCTTTCTCCAACAGTCTTACTTGCATTGGG
AGAGAAGACGCCAGCCTTTT

Product: ATP-dependent Clp protease, subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 867; Mature: 867

Protein sequence:

>867_residues
MEKFSDAVSEALEKAFELAKDAKHSYVTENHLLKSLLQNPGSLFCLVIKDVHGNLGLLTSAVDDALHREPTVVEGAAIPK
PSPSLQQLLLNAQEEARSMGDEYLSGDHLLLAFWKTTKEPFASWRKTVKTSPEALKELIIKLRQGSRMDSPSAEENLKGL
EKYCKNLTILAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIVQGDVPESLKDKHLYVLD
MGALIAGAKYRGEFEERLKSVLKGVEASEGACILFIDEVHTLVGAGATDGAMDAANLLKPALARGTLHCIGATTLNEYQK
YIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFHGVRITEGALNAAVVLSYRYITDRFLPDKAIDLIDEAASLI
RMQIGSLPLPIDEKERELSALIVKQEAIKREQAPAYQEEANEMQKAIDRVKEELAALRLRWDEEKGLIAGLKEKKNSLEN
LKFAEEEAERTADYNRVAELRYSLIPSLEKEIRLAEEALNQRDGRLLQEEVDERLIAQVVANWTGIPVQKMLEGESEKLL
VLEESLEERVVGQPFAITAVSDSIRSARVGLSDPQRPLGVFLFLGPTGVGKTELAKALAELLFNKEEAMIRFDMTEYMEK
HSVSKLIGSPPGYVGYEEGGSLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDDGILTDSKKRKVNCKNALFIMTSNI
GSQELADYCAKKGTIVDKEAVLSVVAPALKNYFSPEFINRIDEILPFVPLTTEDIVKIVGIQMNRVALRLLERRISLTWD
DSVVLFLSEQGYDGAFGARPLKRLIQQKVVTMLSKALLKGDIKSGMSVELTMAKDVVVFKTKTNSVV

Sequences:

>Translated_867_residues
MEKFSDAVSEALEKAFELAKDAKHSYVTENHLLKSLLQNPGSLFCLVIKDVHGNLGLLTSAVDDALHREPTVVEGAAIPK
PSPSLQQLLLNAQEEARSMGDEYLSGDHLLLAFWKTTKEPFASWRKTVKTSPEALKELIIKLRQGSRMDSPSAEENLKGL
EKYCKNLTILAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIVQGDVPESLKDKHLYVLD
MGALIAGAKYRGEFEERLKSVLKGVEASEGACILFIDEVHTLVGAGATDGAMDAANLLKPALARGTLHCIGATTLNEYQK
YIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFHGVRITEGALNAAVVLSYRYITDRFLPDKAIDLIDEAASLI
RMQIGSLPLPIDEKERELSALIVKQEAIKREQAPAYQEEANEMQKAIDRVKEELAALRLRWDEEKGLIAGLKEKKNSLEN
LKFAEEEAERTADYNRVAELRYSLIPSLEKEIRLAEEALNQRDGRLLQEEVDERLIAQVVANWTGIPVQKMLEGESEKLL
VLEESLEERVVGQPFAITAVSDSIRSARVGLSDPQRPLGVFLFLGPTGVGKTELAKALAELLFNKEEAMIRFDMTEYMEK
HSVSKLIGSPPGYVGYEEGGSLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDDGILTDSKKRKVNCKNALFIMTSNI
GSQELADYCAKKGTIVDKEAVLSVVAPALKNYFSPEFINRIDEILPFVPLTTEDIVKIVGIQMNRVALRLLERRISLTWD
DSVVLFLSEQGYDGAFGARPLKRLIQQKVVTMLSKALLKGDIKSGMSVELTMAKDVVVFKTKTNSVV
>Mature_867_residues
MEKFSDAVSEALEKAFELAKDAKHSYVTENHLLKSLLQNPGSLFCLVIKDVHGNLGLLTSAVDDALHREPTVVEGAAIPK
PSPSLQQLLLNAQEEARSMGDEYLSGDHLLLAFWKTTKEPFASWRKTVKTSPEALKELIIKLRQGSRMDSPSAEENLKGL
EKYCKNLTILAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIVQGDVPESLKDKHLYVLD
MGALIAGAKYRGEFEERLKSVLKGVEASEGACILFIDEVHTLVGAGATDGAMDAANLLKPALARGTLHCIGATTLNEYQK
YIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFHGVRITEGALNAAVVLSYRYITDRFLPDKAIDLIDEAASLI
RMQIGSLPLPIDEKERELSALIVKQEAIKREQAPAYQEEANEMQKAIDRVKEELAALRLRWDEEKGLIAGLKEKKNSLEN
LKFAEEEAERTADYNRVAELRYSLIPSLEKEIRLAEEALNQRDGRLLQEEVDERLIAQVVANWTGIPVQKMLEGESEKLL
VLEESLEERVVGQPFAITAVSDSIRSARVGLSDPQRPLGVFLFLGPTGVGKTELAKALAELLFNKEEAMIRFDMTEYMEK
HSVSKLIGSPPGYVGYEEGGSLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDDGILTDSKKRKVNCKNALFIMTSNI
GSQELADYCAKKGTIVDKEAVLSVVAPALKNYFSPEFINRIDEILPFVPLTTEDIVKIVGIQMNRVALRLLERRISLTWD
DSVVLFLSEQGYDGAFGARPLKRLIQQKVVTMLSKALLKGDIKSGMSVELTMAKDVVVFKTKTNSVV

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=317, Percent_Identity=38.801261829653, Blast_Score=227, Evalue=4e-59,
Organism=Escherichia coli, GI1788943, Length=858, Percent_Identity=49.6503496503497, Blast_Score=815, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=426, Percent_Identity=42.2535211267606, Blast_Score=310, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6320464, Length=708, Percent_Identity=51.5536723163842, Blast_Score=707, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=875, Percent_Identity=41.8285714285714, Blast_Score=620, Evalue=1e-178,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB_CHLMU (Q9PKS5)

Other databases:

- EMBL:   AE002160
- PIR:   A81707
- RefSeq:   NP_296767.1
- ProteinModelPortal:   Q9PKS5
- GeneID:   1245741
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0389
- TIGR:   TC_0389
- HOGENOM:   HBG413133
- OMA:   HITESAI
- PhylomeDB:   Q9PKS5
- ProtClustDB:   CLSK871192
- BioCyc:   CMUR243161:TC_0389-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- Gene3D:   G3DSA:1.10.1780.10
- PRINTS:   PR00300
- SMART:   SM00382

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 96606; Mature: 96606

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFSDAVSEALEKAFELAKDAKHSYVTENHLLKSLLQNPGSLFCLVIKDVHGNLGLLTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHH
AVDDALHREPTVVEGAAIPKPSPSLQQLLLNAQEEARSMGDEYLSGDHLLLAFWKTTKEP
HHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEHHCHHH
FASWRKTVKTSPEALKELIIKLRQGSRMDSPSAEENLKGLEKYCKNLTILAREGKLDPVI
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCC
GRDEEIRRTIQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIVQGDVPESLKDKHLYVLD
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEE
MGALIAGAKYRGEFEERLKSVLKGVEASEGACILFIDEVHTLVGAGATDGAMDAANLLKP
HHHHHHCCHHCCHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHH
ALARGTLHCIGATTLNEYQKYIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFH
HHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
GVRITEGALNAAVVLSYRYITDRFLPDKAIDLIDEAASLIRMQIGSLPLPIDEKERELSA
CCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LIVKQEAIKREQAPAYQEEANEMQKAIDRVKEELAALRLRWDEEKGLIAGLKEKKNSLEN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LKFAEEEAERTADYNRVAELRYSLIPSLEKEIRLAEEALNQRDGRLLQEEVDERLIAQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ANWTGIPVQKMLEGESEKLLVLEESLEERVVGQPFAITAVSDSIRSARVGLSDPQRPLGV
HCCCCCCHHHHHCCCCCEEEEEHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCEE
FLFLGPTGVGKTELAKALAELLFNKEEAMIRFDMTEYMEKHSVSKLIGSPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
SLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDDGILTDSKKRKVNCKNALFIMTSNI
CHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCC
GSQELADYCAKKGTIVDKEAVLSVVAPALKNYFSPEFINRIDEILPFVPLTTEDIVKIVG
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHC
IQMNRVALRLLERRISLTWDDSVVLFLSEQGYDGAFGARPLKRLIQQKVVTMLSKALLKG
CHHHHHHHHHHHHHHCCEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DIKSGMSVELTMAKDVVVFKTKTNSVV
HHCCCCEEEEEEECCEEEEEECCCCCC
>Mature Secondary Structure
MEKFSDAVSEALEKAFELAKDAKHSYVTENHLLKSLLQNPGSLFCLVIKDVHGNLGLLTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHH
AVDDALHREPTVVEGAAIPKPSPSLQQLLLNAQEEARSMGDEYLSGDHLLLAFWKTTKEP
HHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEHHCHHH
FASWRKTVKTSPEALKELIIKLRQGSRMDSPSAEENLKGLEKYCKNLTILAREGKLDPVI
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCC
GRDEEIRRTIQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIVQGDVPESLKDKHLYVLD
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEE
MGALIAGAKYRGEFEERLKSVLKGVEASEGACILFIDEVHTLVGAGATDGAMDAANLLKP
HHHHHHCCHHCCHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHH
ALARGTLHCIGATTLNEYQKYIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFH
HHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
GVRITEGALNAAVVLSYRYITDRFLPDKAIDLIDEAASLIRMQIGSLPLPIDEKERELSA
CCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LIVKQEAIKREQAPAYQEEANEMQKAIDRVKEELAALRLRWDEEKGLIAGLKEKKNSLEN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LKFAEEEAERTADYNRVAELRYSLIPSLEKEIRLAEEALNQRDGRLLQEEVDERLIAQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ANWTGIPVQKMLEGESEKLLVLEESLEERVVGQPFAITAVSDSIRSARVGLSDPQRPLGV
HCCCCCCHHHHHCCCCCEEEEEHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCEE
FLFLGPTGVGKTELAKALAELLFNKEEAMIRFDMTEYMEKHSVSKLIGSPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
SLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDDGILTDSKKRKVNCKNALFIMTSNI
CHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCC
GSQELADYCAKKGTIVDKEAVLSVVAPALKNYFSPEFINRIDEILPFVPLTTEDIVKIVG
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHC
IQMNRVALRLLERRISLTWDDSVVLFLSEQGYDGAFGARPLKRLIQQKVVTMLSKALLKG
CHHHHHHHHHHHHHHCCEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DIKSGMSVELTMAKDVVVFKTKTNSVV
HHCCCCEEEEEEECCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935