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The map label for this gene is nusA

Identifier: 15834991

GI number: 15834991

Start: 433627

End: 434931

Strand: Reverse

Name: nusA

Synonym: TC0372

Alternate gene names: 15834991

Gene position: 434931-433627 (Counterclockwise)

Preceding gene: 161579046

Following gene: 15834990

Centisome position: 40.54

GC content: 39.46

Gene sequence:

>1305_bases
ATGAACAAGGATCTTGTGGCTATTTTTGACTACATGGAAAGAGAGAAAGGTATTCAACGCTCTACAATAGTAGGAGCTAT
TGAATCCGCTCTGAAAATTGCTGCGAAAAAGACTCTTAGAGATGACGCGAATGTGTCCGTGAGTATCAATCCTCGCACCG
GAGACATAGAAGTTTTTTGTGAAAAACAGATCGTTGAAAAATGCCAAAATCCAAGTAAGGAAATTCCCTTAGATAAAGCC
CGTGAATATGATCCAGATTGTCAAATCGGGCAATATATGGATGTTCCTTTTGTCTCTGATCAATTTGGAAGAATTGCAGC
TCATGCAGCTCGCCAAATTATCGGTCAGAAACTTCGTCATGCCGAACGCGATGTAATTTATGAAGAGTATCGCCATCGAA
AAAATGAAATCATTTCGGGGGTGATTAAAAGTTTCTCTCGCGGATCAAACTTAGTGGTTGATTTGGGGAAAGTAGAGGGG
TTACTTCCCGCTCGCTTTTATCCTAAAACGGAAAAACATAAAGTAGGCGATAAGATTTATGCGCTTCTTTACGAAGTACA
GGAATCTGAAAATGGCGGAGCTGAAGTTATTCTCAGTAGAAGTCACCCAGAATTTGTAAAACAATTATTCATGCAAGAAG
TCCCAGAATTAGAAGAAGGGTCTGTTGAGATTGTGAAAATTGCAAGAGAAGCTGGCTATCGAACAAAAATGGCAGTGCGC
TCTTCTTCCCCTCAAACAGATGCTGTAGGGGCTTTTGTAGGTATGCGTGGCTCTCGCATCAAAAACATCATTCGAGAATT
AAATGACGAAAAAATAGACGTTGTAAATTATTCCCCTGTATCGACAGAATTGTTACAAAATTTGCTCTATCCTGTAGAGA
TTCAAAAGATCGCTATTTTAGAAGACGATAAAGTTATCGCTATTATCGTCCAAGATTCGGACTATGCAACTGTTATTGGT
AAACGGGGTATCAATGCTCGTCTAATCAGTCAAATTTTGGGCTATGAACTTGAAGTCCAAAGAATGAGTGAATACAACAA
GCTATTAGAAATTCAACGCTTACAGCTAGCTGAATTCGAAGATCCTCGATTGGATCAGCCTTTAGAAGTGGAAGGAATTA
ATACGTTAATAGTGCAGAACTTAGAGCACGCAGGATACGACACGATTAGGAAAATATTGCTGGCTAGTGCTAGCGAATTG
GCTTCTGTTCCAGGAATTAGTTTAGAGTTAGCTTACAAAATCCTCGAACAGGTCAGCAAATATGGAGCATGCAAAGTTGA
CGAAAAACCTAAAGTTGAAGATTAA

Upstream 100 bases:

>100_bases
AATGAGGGATAAACGTAAGAGCAGAGCTAATAAGGCTCTGCTCCATTAGCATTTAGCATTATTTTTTTGTTTTTTTCCCT
TACCCATAAGAGGAGTATTA

Downstream 100 bases:

>100_bases
GAACGCACAGTTAACGAAGGCTGCTGGCTTAGATAAGCTGAAACAGAAACTAGCTCAAGCCGGATCTTCTGACACCAAAA
ATTCTCCAGTAAGTAAAGCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNKDLVAIFDYMEREKGIQRSTIVGAIESALKIAAKKTLRDDANVSVSINPRTGDIEVFCEKQIVEKCQNPSKEIPLDKA
REYDPDCQIGQYMDVPFVSDQFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRKNEIISGVIKSFSRGSNLVVDLGKVEG
LLPARFYPKTEKHKVGDKIYALLYEVQESENGGAEVILSRSHPEFVKQLFMQEVPELEEGSVEIVKIAREAGYRTKMAVR
SSSPQTDAVGAFVGMRGSRIKNIIRELNDEKIDVVNYSPVSTELLQNLLYPVEIQKIAILEDDKVIAIIVQDSDYATVIG
KRGINARLISQILGYELEVQRMSEYNKLLEIQRLQLAEFEDPRLDQPLEVEGINTLIVQNLEHAGYDTIRKILLASASEL
ASVPGISLELAYKILEQVSKYGACKVDEKPKVED

Sequences:

>Translated_434_residues
MNKDLVAIFDYMEREKGIQRSTIVGAIESALKIAAKKTLRDDANVSVSINPRTGDIEVFCEKQIVEKCQNPSKEIPLDKA
REYDPDCQIGQYMDVPFVSDQFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRKNEIISGVIKSFSRGSNLVVDLGKVEG
LLPARFYPKTEKHKVGDKIYALLYEVQESENGGAEVILSRSHPEFVKQLFMQEVPELEEGSVEIVKIAREAGYRTKMAVR
SSSPQTDAVGAFVGMRGSRIKNIIRELNDEKIDVVNYSPVSTELLQNLLYPVEIQKIAILEDDKVIAIIVQDSDYATVIG
KRGINARLISQILGYELEVQRMSEYNKLLEIQRLQLAEFEDPRLDQPLEVEGINTLIVQNLEHAGYDTIRKILLASASEL
ASVPGISLELAYKILEQVSKYGACKVDEKPKVED
>Mature_434_residues
MNKDLVAIFDYMEREKGIQRSTIVGAIESALKIAAKKTLRDDANVSVSINPRTGDIEVFCEKQIVEKCQNPSKEIPLDKA
REYDPDCQIGQYMDVPFVSDQFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRKNEIISGVIKSFSRGSNLVVDLGKVEG
LLPARFYPKTEKHKVGDKIYALLYEVQESENGGAEVILSRSHPEFVKQLFMQEVPELEEGSVEIVKIAREAGYRTKMAVR
SSSPQTDAVGAFVGMRGSRIKNIIRELNDEKIDVVNYSPVSTELLQNLLYPVEIQKIAILEDDKVIAIIVQDSDYATVIG
KRGINARLISQILGYELEVQRMSEYNKLLEIQRLQLAEFEDPRLDQPLEVEGINTLIVQNLEHAGYDTIRKILLASASEL
ASVPGISLELAYKILEQVSKYGACKVDEKPKVED

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=464, Percent_Identity=31.25, Blast_Score=225, Evalue=5e-60,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 48910; Mature: 48910

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDLVAIFDYMEREKGIQRSTIVGAIESALKIAAKKTLRDDANVSVSINPRTGDIEVFC
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEE
EKQIVEKCQNPSKEIPLDKAREYDPDCQIGQYMDVPFVSDQFGRIAAHAARQIIGQKLRH
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AERDVIYEEYRHRKNEIISGVIKSFSRGSNLVVDLGKVEGLLPARFYPKTEKHKVGDKIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCCCCCCCHHHHHHHHHH
ALLYEVQESENGGAEVILSRSHPEFVKQLFMQEVPELEEGSVEIVKIAREAGYRTKMAVR
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEC
SSSPQTDAVGAFVGMRGSRIKNIIRELNDEKIDVVNYSPVSTELLQNLLYPVEIQKIAIL
CCCCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEE
EDDKVIAIIVQDSDYATVIGKRGINARLISQILGYELEVQRMSEYNKLLEIQRLQLAEFE
ECCCEEEEEEECCCCEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
DPRLDQPLEVEGINTLIVQNLEHAGYDTIRKILLASASELASVPGISLELAYKILEQVSK
CCCCCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YGACKVDEKPKVED
CCCCCCCCCCCCCC
>Mature Secondary Structure
MNKDLVAIFDYMEREKGIQRSTIVGAIESALKIAAKKTLRDDANVSVSINPRTGDIEVFC
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEE
EKQIVEKCQNPSKEIPLDKAREYDPDCQIGQYMDVPFVSDQFGRIAAHAARQIIGQKLRH
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AERDVIYEEYRHRKNEIISGVIKSFSRGSNLVVDLGKVEGLLPARFYPKTEKHKVGDKIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCCCCCCCHHHHHHHHHH
ALLYEVQESENGGAEVILSRSHPEFVKQLFMQEVPELEEGSVEIVKIAREAGYRTKMAVR
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEC
SSSPQTDAVGAFVGMRGSRIKNIIRELNDEKIDVVNYSPVSTELLQNLLYPVEIQKIAIL
CCCCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEE
EDDKVIAIIVQDSDYATVIGKRGINARLISQILGYELEVQRMSEYNKLLEIQRLQLAEFE
ECCCEEEEEEECCCCEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
DPRLDQPLEVEGINTLIVQNLEHAGYDTIRKILLASASELASVPGISLELAYKILEQVSK
CCCCCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YGACKVDEKPKVED
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]