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The map label for this gene is folD

Identifier: 15834969

GI number: 15834969

Start: 413012

End: 413875

Strand: Reverse

Name: folD

Synonym: TC0350

Alternate gene names: 15834969

Gene position: 413875-413012 (Counterclockwise)

Preceding gene: 15834970

Following gene: 15834968

Centisome position: 38.57

GC content: 41.2

Gene sequence:

>864_bases
ATGTTATTAAAGGGAGCTCCAGCCGCTGATGGTATTCTAGCAACTGTCAAAGACAATATCCGATCCCAATCAGGTTCTCC
TGGTCTTGCTGTTGTCTTGATAGGCAACAACCCTGCTTCAGAAATTTATGTAAATATGAAAGTAAAGCGCGCCAGAGATT
TGGGAATGATATCCAGATCTTACCGGAAGCCTTCGGATGCAACTCTTTCTGATATTCTATCTCTTATCCACGAGCTAAAC
TGCGATGAAAATATTCATGGCGTTCTAGTTCAACTCCCCCTTCCTAAACATCTAGATACTCAAACAATCCTTTCTTCTAT
TTCCCCTAATAAGGATGTTGATGGTTTACACCCTGTGAATATGGGGAAACTTCTTCTTGGAGAAACGGATGGGTTTATCC
CCTGTACCCCCTCAGGAATTGTTGAACTTTTCAAATATTATGAAATTCCTCTCTATGGAAAACACGTTGTCATCTTGGGA
CGTAGTAATATTGTAGGGAAACCTTTATCAGCATTGCTTATGCAAAAACATGCAGATACTAATGCTAGCGTGACAGTTCT
TCATAGCCAATCCGAACATCTAAAAGAAATCACTAAAACTGCAGACATTCTTGTCTCTGCAATTGGTGTTCCTCTCTTTG
TAACTAAGGAAATGATCTCAGAAAAATGTATCGTTATAGATGTTGGAACTTCAAGAGTCCCTGCAGACAACCCTAAAGGG
TATTCTTTAGTGGGCGATGTCGATTTTAACAATGTCGTTCCTGTTTGCCAAGCTATTACCCCTGTCCCTGGTGGGGTGGG
CCCCATGACCGTTGCCATGCTAATGAGAAATACATGGGAAAGTTTTTTACGCCATACGCTTTGA

Upstream 100 bases:

>100_bases
AGTTTTAAAAATTCATCAGCTGCTGTACACTGATCGAGAGGATTGTATCCCCTATTTTCTAGAACAACTGACAATAAATC
ATTTAACTAAGTGTTTGTAC

Downstream 100 bases:

>100_bases
TCATATTCGTTTTCTTGATTCAAGCCTGCTCTTCTCCTTCAAAAACTATTTTTGAAGGTGTCCGCATGACAATTCCTTAC
CGAGTCGTGCTCGGGGAAAC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLLKGAPAADGILATVKDNIRSQSGSPGLAVVLIGNNPASEIYVNMKVKRARDLGMISRSYRKPSDATLSDILSLIHELN
CDENIHGVLVQLPLPKHLDTQTILSSISPNKDVDGLHPVNMGKLLLGETDGFIPCTPSGIVELFKYYEIPLYGKHVVILG
RSNIVGKPLSALLMQKHADTNASVTVLHSQSEHLKEITKTADILVSAIGVPLFVTKEMISEKCIVIDVGTSRVPADNPKG
YSLVGDVDFNNVVPVCQAITPVPGGVGPMTVAMLMRNTWESFLRHTL

Sequences:

>Translated_287_residues
MLLKGAPAADGILATVKDNIRSQSGSPGLAVVLIGNNPASEIYVNMKVKRARDLGMISRSYRKPSDATLSDILSLIHELN
CDENIHGVLVQLPLPKHLDTQTILSSISPNKDVDGLHPVNMGKLLLGETDGFIPCTPSGIVELFKYYEIPLYGKHVVILG
RSNIVGKPLSALLMQKHADTNASVTVLHSQSEHLKEITKTADILVSAIGVPLFVTKEMISEKCIVIDVGTSRVPADNPKG
YSLVGDVDFNNVVPVCQAITPVPGGVGPMTVAMLMRNTWESFLRHTL
>Mature_287_residues
MLLKGAPAADGILATVKDNIRSQSGSPGLAVVLIGNNPASEIYVNMKVKRARDLGMISRSYRKPSDATLSDILSLIHELN
CDENIHGVLVQLPLPKHLDTQTILSSISPNKDVDGLHPVNMGKLLLGETDGFIPCTPSGIVELFKYYEIPLYGKHVVILG
RSNIVGKPLSALLMQKHADTNASVTVLHSQSEHLKEITKTADILVSAIGVPLFVTKEMISEKCIVIDVGTSRVPADNPKG
YSLVGDVDFNNVVPVCQAITPVPGGVGPMTVAMLMRNTWESFLRHTL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=263, Percent_Identity=45.2471482889734, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI222418558, Length=258, Percent_Identity=43.7984496124031, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI94721354, Length=269, Percent_Identity=45.3531598513011, Blast_Score=204, Evalue=8e-53,
Organism=Homo sapiens, GI36796743, Length=181, Percent_Identity=26.5193370165746, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786741, Length=270, Percent_Identity=44.4444444444444, Blast_Score=216, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17568735, Length=265, Percent_Identity=42.2641509433962, Blast_Score=183, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=283, Percent_Identity=42.4028268551237, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=40.6779661016949, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322933, Length=298, Percent_Identity=26.1744966442953, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=41.5807560137457, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=41.5807560137457, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=41.5807560137457, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=41.5807560137457, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17136818, Length=269, Percent_Identity=40.8921933085502, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI17136816, Length=269, Percent_Identity=40.8921933085502, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CHLMU (Q9PKW1)

Other databases:

- EMBL:   AE002160
- PIR:   F81713
- RefSeq:   NP_296728.1
- ProteinModelPortal:   Q9PKW1
- SMR:   Q9PKW1
- GeneID:   1245703
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0350
- TIGR:   TC_0350
- HOGENOM:   HBG328751
- OMA:   ELPEDCT
- PhylomeDB:   Q9PKW1
- ProtClustDB:   PRK14181
- BioCyc:   CMUR243161:TC_0350-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30916; Mature: 30916

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKGAPAADGILATVKDNIRSQSGSPGLAVVLIGNNPASEIYVNMKVKRARDLGMISRS
CCCCCCCCCCCEEHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEEHHHHHHHHHHHH
YRKPSDATLSDILSLIHELNCDENIHGVLVQLPLPKHLDTQTILSSISPNKDVDGLHPVN
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCC
MGKLLLGETDGFIPCTPSGIVELFKYYEIPLYGKHVVILGRSNIVGKPLSALLMQKHADT
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCC
NASVTVLHSQSEHLKEITKTADILVSAIGVPLFVTKEMISEKCIVIDVGTSRVPADNPKG
CCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCEEEEECCCCCCCCCCCCC
YSLVGDVDFNNVVPVCQAITPVPGGVGPMTVAMLMRNTWESFLRHTL
EEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLKGAPAADGILATVKDNIRSQSGSPGLAVVLIGNNPASEIYVNMKVKRARDLGMISRS
CCCCCCCCCCCEEHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEEHHHHHHHHHHHH
YRKPSDATLSDILSLIHELNCDENIHGVLVQLPLPKHLDTQTILSSISPNKDVDGLHPVN
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCC
MGKLLLGETDGFIPCTPSGIVELFKYYEIPLYGKHVVILGRSNIVGKPLSALLMQKHADT
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCC
NASVTVLHSQSEHLKEITKTADILVSAIGVPLFVTKEMISEKCIVIDVGTSRVPADNPKG
CCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHEEEHHHHCCCEEEEECCCCCCCCCCCCC
YSLVGDVDFNNVVPVCQAITPVPGGVGPMTVAMLMRNTWESFLRHTL
EEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935