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Identifier: 15834968

GI number: 15834968

Start: 412092

End: 413111

Strand: Reverse

Name: Not Available

Synonym: TC0349

Alternate gene names: 15834968

Gene position: 413111-412092 (Counterclockwise)

Preceding gene: 15834969

Following gene: 15834966

Centisome position: 38.5

GC content: 39.12

Gene sequence:

>1020_bases
TTGCCAAGCTATTACCCCTGTCCCTGGTGGGGTGGGCCCCATGACCGTTGCCATGCTAATGAGAAATACATGGGAAAGTT
TTTTACGCCATACGCTTTGATCATATTCGTTTTCTTGATTCAAGCCTGCTCTTCTCCTTCAAAAACTATTTTTGAAGGTG
TCCGCATGACAATTCCTTACCGAGTCGTGCTCGGGGAAACTCTTTCCTTTTCTCAAAAAAAACAAGCTCAAAAGGAAATC
GATCAAATATTTGATCACATTGACCAAATATTTAATAATTGGAATCCTTTCTCTGAAATTTCGCGCATTAATCGTTCTGA
AACTCTTGAACCCATTTCCCTATCTCCAGAACTCTTCTCTTTTTTATGTGAAATAGATCGATTCCATACCTTTTCAGACG
GACGTTTTGATCCTACCTTAGGAGCTTTAAAAAACTTATGGCTGCTTCACTTAAAATCACAGACTCTCCCCCCTCAAGAA
CTACTTCACTCGTATAAGCAAAACACTGGATGGCATCTACTTTCTCTTGATAAGACCAATCACACGCTAAAAAAACTGTC
TCCTTCAGTCCAATTAGATCTCTGTGGAGCTGTAAAAGGATTCGCTGTAGATCTGTTAGGAATATTTTGTTCCCAATTTT
GTCAAAATTATTACGTGGAATGGGGTGGAGAAATCAAGACATCAGGAAAGCACCCTTCTGGTAGGTCTTGGGCCATAGCA
TCATCAGCAACGCCAGAAATTCTTCATCTCAATAATAGCTCCATAGCAACTAGTGGAAACCAATACCAACGTTGGTATGT
AAATAAAAAAATTTACACTCATATTTTGGATCCTTTAACAGGAATTCCTCTAGAAGAGAGTAGCTATCCTATCTTAGCAG
TATCTGTAATCGATGAAAATTGTGCTTTTGCAGATGCTATGGCTACAGCATTAACTACCTTCACCTCTAAGCAAGAAGCT
CTTGATTGGGCAAAAAAGAAACATCTTTGTGTCTATATTACCGATAAGAACGCTTCATAG

Upstream 100 bases:

>100_bases
AATGTATCGTTATAGATGTTGGAACTTCAAGAGTCCCTGCAGACAACCCTAAAGGGTATTCTTTAGTGGGCGATGTCGAT
TTTAACAATGTCGTTCCTGT

Downstream 100 bases:

>100_bases
CGGCAGCTAATTCACGATTCTTTTCTCGTTCAATGAGTGCCTGACGCTTATCGTGGGATTTTTTCCCTCGACAACAGCCA
ATACGCACTTTAACAAAACC

Product: thiamine biosynthesis lipoprotein, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MPSYYPCPWWGGPHDRCHANEKYMGKFFTPYALIIFVFLIQACSSPSKTIFEGVRMTIPYRVVLGETLSFSQKKQAQKEI
DQIFDHIDQIFNNWNPFSEISRINRSETLEPISLSPELFSFLCEIDRFHTFSDGRFDPTLGALKNLWLLHLKSQTLPPQE
LLHSYKQNTGWHLLSLDKTNHTLKKLSPSVQLDLCGAVKGFAVDLLGIFCSQFCQNYYVEWGGEIKTSGKHPSGRSWAIA
SSATPEILHLNNSSIATSGNQYQRWYVNKKIYTHILDPLTGIPLEESSYPILAVSVIDENCAFADAMATALTTFTSKQEA
LDWAKKKHLCVYITDKNAS

Sequences:

>Translated_339_residues
MPSYYPCPWWGGPHDRCHANEKYMGKFFTPYALIIFVFLIQACSSPSKTIFEGVRMTIPYRVVLGETLSFSQKKQAQKEI
DQIFDHIDQIFNNWNPFSEISRINRSETLEPISLSPELFSFLCEIDRFHTFSDGRFDPTLGALKNLWLLHLKSQTLPPQE
LLHSYKQNTGWHLLSLDKTNHTLKKLSPSVQLDLCGAVKGFAVDLLGIFCSQFCQNYYVEWGGEIKTSGKHPSGRSWAIA
SSATPEILHLNNSSIATSGNQYQRWYVNKKIYTHILDPLTGIPLEESSYPILAVSVIDENCAFADAMATALTTFTSKQEA
LDWAKKKHLCVYITDKNAS
>Mature_338_residues
PSYYPCPWWGGPHDRCHANEKYMGKFFTPYALIIFVFLIQACSSPSKTIFEGVRMTIPYRVVLGETLSFSQKKQAQKEID
QIFDHIDQIFNNWNPFSEISRINRSETLEPISLSPELFSFLCEIDRFHTFSDGRFDPTLGALKNLWLLHLKSQTLPPQEL
LHSYKQNTGWHLLSLDKTNHTLKKLSPSVQLDLCGAVKGFAVDLLGIFCSQFCQNYYVEWGGEIKTSGKHPSGRSWAIAS
SATPEILHLNNSSIATSGNQYQRWYVNKKIYTHILDPLTGIPLEESSYPILAVSVIDENCAFADAMATALTTFTSKQEAL
DWAKKKHLCVYITDKNAS

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=332, Percent_Identity=26.2048192771084, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38503; Mature: 38372

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSYYPCPWWGGPHDRCHANEKYMGKFFTPYALIIFVFLIQACSSPSKTIFEGVRMTIPY
CCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHCCCE
RVVLGETLSFSQKKQAQKEIDQIFDHIDQIFNNWNPFSEISRINRSETLEPISLSPELFS
EEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCCCCCHHHHH
FLCEIDRFHTFSDGRFDPTLGALKNLWLLHLKSQTLPPQELLHSYKQNTGWHLLSLDKTN
HHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCC
HTLKKLSPSVQLDLCGAVKGFAVDLLGIFCSQFCQNYYVEWGGEIKTSGKHPSGRSWAIA
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCCCEEEE
SSATPEILHLNNSSIATSGNQYQRWYVNKKIYTHILDPLTGIPLEESSYPILAVSVIDEN
CCCCCCEEEECCCCEECCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCC
CAFADAMATALTTFTSKQEALDWAKKKHLCVYITDKNAS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
>Mature Secondary Structure 
PSYYPCPWWGGPHDRCHANEKYMGKFFTPYALIIFVFLIQACSSPSKTIFEGVRMTIPY
CCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHCCCE
RVVLGETLSFSQKKQAQKEIDQIFDHIDQIFNNWNPFSEISRINRSETLEPISLSPELFS
EEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCCCCCHHHHH
FLCEIDRFHTFSDGRFDPTLGALKNLWLLHLKSQTLPPQELLHSYKQNTGWHLLSLDKTN
HHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCC
HTLKKLSPSVQLDLCGAVKGFAVDLLGIFCSQFCQNYYVEWGGEIKTSGKHPSGRSWAIA
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCCCEEEE
SSATPEILHLNNSSIATSGNQYQRWYVNKKIYTHILDPLTGIPLEESSYPILAVSVIDEN
CCCCCCEEEECCCCEECCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCC
CAFADAMATALTTFTSKQEALDWAKKKHLCVYITDKNAS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]