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The map label for this gene is dnaN

Identifier: 15834966

GI number: 15834966

Start: 410298

End: 411398

Strand: Reverse

Name: dnaN

Synonym: TC0347

Alternate gene names: 15834966

Gene position: 411398-410298 (Counterclockwise)

Preceding gene: 15834968

Following gene: 15834965

Centisome position: 38.34

GC content: 40.42

Gene sequence:

>1101_bases
ATGAAATTCGTTATCTCCCGAAATGAGTTAGGAAATCTGATTAAAAAGGTTCAAAACGTTGTTCCGCAAAGCACACCAAT
TCCCGTGCTAACCCATGTACTCATAGAGAGTTGCAACGATGAATTAGTGTTCACTGCTACAGATTTAACAGTCAGTACTC
GTTGTGTTGTTAAAGCGAAAGTATATGAATCTGGGTCTGTAACTATACCTTCTCGACGTTTTTTCCAACTCATTCGAGAG
TTAACAGAAGCTAACATTGAAGTGGCGGCTAATTCTGGAGAGATGGCAACGATTACCTCTGGATCTTCGTGTTTTAGACT
GCTAAGTATGGGGAAGGAAGATTTCCCCATGCTTCCTGACATGCAAAACGCTCTTCGTTTCACCCTTGATTCTGAGCAAT
TAAAGGATATGTTCCAAAGAACATCTTTTGCAGTATCTCGAGAAGAGAGTCGCTACGTACTTACCGGGGTTTTGCTTTCC
ATCTCTAACGGCACCATGACTGTTGTCGGAACCGATGGTAAGCGTTTAGCAAAAATCGACACTAATATTTCTTTGGATCC
AAGCTTTTCAGGAGACTATATTATTCCTATCAAGGCGGTTGAAGAGATCATTCGCATGGCTTCGGAAGATGTCCAATCAA
CGATCTTTTTAGATCAAACTAAAATTGCTGTAGAATGTGGAAACACTCTGTTAGTGACTAAACTCCTTTCTGGAGAATTC
CCAGACTTTTCCCCTGTGATTTCTACACAGAGTAGCGTTCAATTAAACCTTCATCGTGAAGAGCTTATTTCCCTTCTTAA
GCAAGTAGCTCTTTTCACAAACGAGTCCTCCCATTCTGTAAAATTTAGCTTTTCTCCAGGAGAACTTACTCTTACAGCAA
ACTGTACAAAAGTCGGAGAAGGGAAGGTGAGTATGGCCGTGAATTACACGGGTGAAACTCTAGAAATAGCCTTCAATCCT
TTCTTCTTTTTAGACATACTGAAACACAGCCGTGATGAACTTGTTCAATTAGGAATCTCCGATTCCTATAATCCTGGGAT
TATTACAGATTCCACACGCAGTTTGTTTGTCATTATGCCTATGAGATTACATGACGATTAA

Upstream 100 bases:

>100_bases
ATTTTTTATAGATTTGGCCCCCGGGAGACGATTTATTTACCTCCGCGCCGCAAAGCTCGGTTGTTCATTCCAAAAAGACT
TACGTAATTACAGGAAAGTA

Downstream 100 bases:

>100_bases
TGCGAGTTCATTCTTTATTTCTTAAAGATTTCAGAAATTACTCGGAACTGCGTCTAGAATTAGGACCAGAGATGAATTCT
ATCTTTGGTCTTAATGCTCA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFVISRNELGNLIKKVQNVVPQSTPIPVLTHVLIESCNDELVFTATDLTVSTRCVVKAKVYESGSVTIPSRRFFQLIRE
LTEANIEVAANSGEMATITSGSSCFRLLSMGKEDFPMLPDMQNALRFTLDSEQLKDMFQRTSFAVSREESRYVLTGVLLS
ISNGTMTVVGTDGKRLAKIDTNISLDPSFSGDYIIPIKAVEEIIRMASEDVQSTIFLDQTKIAVECGNTLLVTKLLSGEF
PDFSPVISTQSSVQLNLHREELISLLKQVALFTNESSHSVKFSFSPGELTLTANCTKVGEGKVSMAVNYTGETLEIAFNP
FFFLDILKHSRDELVQLGISDSYNPGIITDSTRSLFVIMPMRLHDD

Sequences:

>Translated_366_residues
MKFVISRNELGNLIKKVQNVVPQSTPIPVLTHVLIESCNDELVFTATDLTVSTRCVVKAKVYESGSVTIPSRRFFQLIRE
LTEANIEVAANSGEMATITSGSSCFRLLSMGKEDFPMLPDMQNALRFTLDSEQLKDMFQRTSFAVSREESRYVLTGVLLS
ISNGTMTVVGTDGKRLAKIDTNISLDPSFSGDYIIPIKAVEEIIRMASEDVQSTIFLDQTKIAVECGNTLLVTKLLSGEF
PDFSPVISTQSSVQLNLHREELISLLKQVALFTNESSHSVKFSFSPGELTLTANCTKVGEGKVSMAVNYTGETLEIAFNP
FFFLDILKHSRDELVQLGISDSYNPGIITDSTRSLFVIMPMRLHDD
>Mature_366_residues
MKFVISRNELGNLIKKVQNVVPQSTPIPVLTHVLIESCNDELVFTATDLTVSTRCVVKAKVYESGSVTIPSRRFFQLIRE
LTEANIEVAANSGEMATITSGSSCFRLLSMGKEDFPMLPDMQNALRFTLDSEQLKDMFQRTSFAVSREESRYVLTGVLLS
ISNGTMTVVGTDGKRLAKIDTNISLDPSFSGDYIIPIKAVEEIIRMASEDVQSTIFLDQTKIAVECGNTLLVTKLLSGEF
PDFSPVISTQSSVQLNLHREELISLLKQVALFTNESSHSVKFSFSPGELTLTANCTKVGEGKVSMAVNYTGETLEIAFNP
FFFLDILKHSRDELVQLGISDSYNPGIITDSTRSLFVIMPMRLHDD

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=369, Percent_Identity=30.8943089430894, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40494; Mature: 40494

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVISRNELGNLIKKVQNVVPQSTPIPVLTHVLIESCNDELVFTATDLTVSTRCVVKAK
CEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEEEE
VYESGSVTIPSRRFFQLIRELTEANIEVAANSGEMATITSGSSCFRLLSMGKEDFPMLPD
EECCCCEECCCHHHHHHHHHHHHCCEEEEECCCCEEEEECCHHHHHHHHCCCCCCCCCCC
MQNALRFTLDSEQLKDMFQRTSFAVSREESRYVLTGVLLSISNGTMTVVGTDGKRLAKID
CHHHHEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEEECCCCEEEEEC
TNISLDPSFSGDYIIPIKAVEEIIRMASEDVQSTIFLDQTKIAVECGNTLLVTKLLSGEF
CCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHEEEEECEEEEEECCCEEEEEHHHCCCC
PDFSPVISTQSSVQLNLHREELISLLKQVALFTNESSHSVKFSFSPGELTLTANCTKVGE
CCCCCHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCEECCC
GKVSMAVNYTGETLEIAFNPFFFLDILKHSRDELVQLGISDSYNPGIITDSTRSLFVIMP
CEEEEEEEECCCEEEEEECCHHHHHHHHCCHHHEEEECCCCCCCCCEEECCCCEEEEEEE
MRLHDD
EEECCC
>Mature Secondary Structure
MKFVISRNELGNLIKKVQNVVPQSTPIPVLTHVLIESCNDELVFTATDLTVSTRCVVKAK
CEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEEEE
VYESGSVTIPSRRFFQLIRELTEANIEVAANSGEMATITSGSSCFRLLSMGKEDFPMLPD
EECCCCEECCCHHHHHHHHHHHHCCEEEEECCCCEEEEECCHHHHHHHHCCCCCCCCCCC
MQNALRFTLDSEQLKDMFQRTSFAVSREESRYVLTGVLLSISNGTMTVVGTDGKRLAKID
CHHHHEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEEECCCCEEEEEC
TNISLDPSFSGDYIIPIKAVEEIIRMASEDVQSTIFLDQTKIAVECGNTLLVTKLLSGEF
CCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHEEEEECEEEEEECCCEEEEEHHHCCCC
PDFSPVISTQSSVQLNLHREELISLLKQVALFTNESSHSVKFSFSPGELTLTANCTKVGE
CCCCCHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCEECCC
GKVSMAVNYTGETLEIAFNPFFFLDILKHSRDELVQLGISDSYNPGIITDSTRSLFVIMP
CEEEEEEEECCCEEEEEECCHHHHHHHHCCHHHEEEECCCCCCCCCEEECCCCEEEEEEE
MRLHDD
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9784136 [H]