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The map label for this gene is recF

Identifier: 15834965

GI number: 15834965

Start: 409201

End: 410298

Strand: Reverse

Name: recF

Synonym: TC0346

Alternate gene names: 15834965

Gene position: 410298-409201 (Counterclockwise)

Preceding gene: 15834966

Following gene: 15834964

Centisome position: 38.24

GC content: 37.07

Gene sequence:

>1098_bases
ATGCGAGTTCATTCTTTATTTCTTAAAGATTTCAGAAATTACTCGGAACTGCGTCTAGAATTAGGACCAGAGATGAATTC
TATCTTTGGTCTTAATGCTCAGGGGAAAACAAATATTCTTGAGGCATTGTATATCTTATCTTTAGGAAGATCCTTCCGAA
CAAGTCGTTTGACTGAGGCTATACGCTTTGGATCGTCTCATTTTTTTATCGAAGCTGTTTTCTCCCAAAACCAAGTTTTT
CATACCCTCTCTATTCAAGTAGATAAACGAGGGAAGAAAATTCTTTTTGATGGAGCCCCTATTACTAAGTTGTCCGCACT
AGTAGGACTATTTCCTGTTATTCTTTTTTCTGTAAAAGACACAACAATTATTGAAGGCTCCCCAGCAGAACGGCGCCGGT
TTCTAGATCTCTTATTAGCCCAAGCTTCAGAAAAATATACAGGTCAGATTGCCTTGTATCACAAAGCTTTGGACCAACGT
AATGCAGCTATTAAAACACAAGACTATAAAACCATCGCTGCCTGGAACTCCCCTCTTATTGCTTATGGAAGCCTGGTAGC
CTTGTTGCGCTATGAATGCGCTAAAAAACTGCATAAAATTTTTCAAAATCTTTGGGATAACACATTAAAAGAAACTCTTT
CTCTTCGTTATGAGAGTTCTCTCATTACAACAGAGTCTCCTACACTCAATGACATTGCAAGTAATTACTATGAACAACTT
AGATTAGCTAATACCAAAGACTTTGAACTTGGCTACACAACAGTAGGCCCTCATCGAGATGAACTTATCATCACGCTTAA
TGATTTACCTGTTTCTAAATTCTCTAGTGAAGGCCAAAAACATTCTCTTTTAGCAGTTCTTCGATTTGCTGAATGTGTAT
ATCTTCAGGAAGAATTTCTTATCCATCCACTTCTATGTATGGATGATATTCACGCTTGTTTAGATCAAAATCGATTAGAT
CAGCTCTTCCAACTCTCAACTTCTTTAGGACAAACTGTTACTACTTCCACCATTTGTCCGAATCATCTAGATTCTAACTC
TTCTATTTTTCACGTTACACAAGCTCAAGTATCGCTTGTCACTTCCAATTTTTTATAA

Upstream 100 bases:

>100_bases
TTGTTCAATTAGGAATCTCCGATTCCTATAATCCTGGGATTATTACAGATTCCACACGCAGTTTGTTTGTCATTATGCCT
ATGAGATTACATGACGATTA

Downstream 100 bases:

>100_bases
TTCTTTATTCGCAATATTTGTTTTATTAATCGAAACAAACTTTTTAGTTTTATTTTATTAATAAAAAGATATTTAGATCG
CATAAACAAAACAATCAAAC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MRVHSLFLKDFRNYSELRLELGPEMNSIFGLNAQGKTNILEALYILSLGRSFRTSRLTEAIRFGSSHFFIEAVFSQNQVF
HTLSIQVDKRGKKILFDGAPITKLSALVGLFPVILFSVKDTTIIEGSPAERRRFLDLLLAQASEKYTGQIALYHKALDQR
NAAIKTQDYKTIAAWNSPLIAYGSLVALLRYECAKKLHKIFQNLWDNTLKETLSLRYESSLITTESPTLNDIASNYYEQL
RLANTKDFELGYTTVGPHRDELIITLNDLPVSKFSSEGQKHSLLAVLRFAECVYLQEEFLIHPLLCMDDIHACLDQNRLD
QLFQLSTSLGQTVTTSTICPNHLDSNSSIFHVTQAQVSLVTSNFL

Sequences:

>Translated_365_residues
MRVHSLFLKDFRNYSELRLELGPEMNSIFGLNAQGKTNILEALYILSLGRSFRTSRLTEAIRFGSSHFFIEAVFSQNQVF
HTLSIQVDKRGKKILFDGAPITKLSALVGLFPVILFSVKDTTIIEGSPAERRRFLDLLLAQASEKYTGQIALYHKALDQR
NAAIKTQDYKTIAAWNSPLIAYGSLVALLRYECAKKLHKIFQNLWDNTLKETLSLRYESSLITTESPTLNDIASNYYEQL
RLANTKDFELGYTTVGPHRDELIITLNDLPVSKFSSEGQKHSLLAVLRFAECVYLQEEFLIHPLLCMDDIHACLDQNRLD
QLFQLSTSLGQTVTTSTICPNHLDSNSSIFHVTQAQVSLVTSNFL
>Mature_365_residues
MRVHSLFLKDFRNYSELRLELGPEMNSIFGLNAQGKTNILEALYILSLGRSFRTSRLTEAIRFGSSHFFIEAVFSQNQVF
HTLSIQVDKRGKKILFDGAPITKLSALVGLFPVILFSVKDTTIIEGSPAERRRFLDLLLAQASEKYTGQIALYHKALDQR
NAAIKTQDYKTIAAWNSPLIAYGSLVALLRYECAKKLHKIFQNLWDNTLKETLSLRYESSLITTESPTLNDIASNYYEQL
RLANTKDFELGYTTVGPHRDELIITLNDLPVSKFSSEGQKHSLLAVLRFAECVYLQEEFLIHPLLCMDDIHACLDQNRLD
QLFQLSTSLGQTVTTSTICPNHLDSNSSIFHVTQAQVSLVTSNFL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=365, Percent_Identity=27.3972602739726, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CHLMU (Q9PKW5)

Other databases:

- EMBL:   AE002160
- PIR:   B81713
- RefSeq:   NP_296724.1
- ProteinModelPortal:   Q9PKW5
- SMR:   Q9PKW5
- GeneID:   1246389
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0346
- TIGR:   TC_0346
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q9PKW5
- ProtClustDB:   PRK00064
- BioCyc:   CMUR243161:TC_0346-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41322; Mature: 41322

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVHSLFLKDFRNYSELRLELGPEMNSIFGLNAQGKTNILEALYILSLGRSFRTSRLTEA
CCHHHHHHHHHCCHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
IRFGSSHFFIEAVFSQNQVFHTLSIQVDKRGKKILFDGAPITKLSALVGLFPVILFSVKD
HHCCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
TTIIEGSPAERRRFLDLLLAQASEKYTGQIALYHKALDQRNAAIKTQDYKTIAAWNSPLI
CEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCEEECCCEEEEECCCCHH
AYGSLVALLRYECAKKLHKIFQNLWDNTLKETLSLRYESSLITTESPTLNDIASNYYEQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
RLANTKDFELGYTTVGPHRDELIITLNDLPVSKFSSEGQKHSLLAVLRFAECVYLQEEFL
HCCCCCCCCCCCEECCCCCCEEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IHPLLCMDDIHACLDQNRLDQLFQLSTSLGQTVTTSTICPNHLDSNSSIFHVTQAQVSLV
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEEHHHHHHH
TSNFL
HHCCC
>Mature Secondary Structure
MRVHSLFLKDFRNYSELRLELGPEMNSIFGLNAQGKTNILEALYILSLGRSFRTSRLTEA
CCHHHHHHHHHCCHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
IRFGSSHFFIEAVFSQNQVFHTLSIQVDKRGKKILFDGAPITKLSALVGLFPVILFSVKD
HHCCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
TTIIEGSPAERRRFLDLLLAQASEKYTGQIALYHKALDQRNAAIKTQDYKTIAAWNSPLI
CEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCEEECCCEEEEECCCCHH
AYGSLVALLRYECAKKLHKIFQNLWDNTLKETLSLRYESSLITTESPTLNDIASNYYEQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
RLANTKDFELGYTTVGPHRDELIITLNDLPVSKFSSEGQKHSLLAVLRFAECVYLQEEFL
HCCCCCCCCCCCEECCCCCCEEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IHPLLCMDDIHACLDQNRLDQLFQLSTSLGQTVTTSTICPNHLDSNSSIFHVTQAQVSLV
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEEHHHHHHH
TSNFL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935