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Identifier: 15834963

GI number: 15834963

Start: 406259

End: 408118

Strand: Direct

Name: Not Available

Synonym: TC0344

Alternate gene names: 15834963

Gene position: 406259-408118 (Clockwise)

Preceding gene: 15834962

Following gene: 15834967

Centisome position: 37.86

GC content: 41.88

Gene sequence:

>1860_bases
ATGACAGTAATATATTTTGTTCTTGCAGCCCTGGCCTTAGGCTTTCTGATTTTAATTCATGAACTAGGCCATTTGTTAGC
AGCGAAAGCTGTAGGAATGACTGTAGAAAGTTTCAGTATAGGGTTTGGACCTGCACTTGTTCGAAAAAAAATGGGGAGTA
TTGAATACCGGATAGGGGCAATTCCTTTTGGAGGATATGTTCGTATCAAAGGTATGGACAGAAATGATAAAGAAATATCT
GAAGACAGGGAGAAAACGGTTTATGATATCCCTGGAGGATTTTTTAGTAAGTCTCCATGGAAACGTATCTTCGTTTTAGC
TGCAGGTCCTCTAGCTAATATACTGGTGGCCCTATTTGCTTTCGGGATTCTCTATTTTTCTGGAGGAAGAACAAAACCTT
TTTCTGAACACACCAGTATTGTTGGGTGGGCTCATCCTTCTTTGGAGCAAAAGGGATTACGTCCAGGGGATCGTATTTTC
TTGTGTAATGGGCAGGTCTACTCTGGGAATAAGATGGCGTTTTCGTCTTCTTTGCTTGATAGAAAATTATCATTGCAAGG
GGAGCATCCTGCTTACTTCTCTGAAGCGGAGTCATTTTCTCTAGACGTACCATTTAATCCTAGCTTAGAAGGAGTCCCTT
GTTTAGGGGCTAGCTATTTACTGTATCGTGGTTCTGAGCCTTTGCCTGAAAAATCTCCTTTGATAGATGCAGGATTATCT
GAAGGGGATCGTCTAGTATGGATGGACGGTGCTTTGGTATTTTCTGGCGTGCAGGTTTCTCAAATTCTCAATGAGAAAAA
AGCTTTTTTGCGAATCGAACGTCAGGGAAAGATCGTATTCGTTCGTCAATCTAGGGTTTTGGCGGGGGATCTTCAGCTCA
CTTCATATTTTAAGAATGAGCTGATTGATTGTCAGTATGAGGCGGGGCTTAAAGGAAAATGGGCTTCTCTATACATGTTG
CCTTACATTATTAATAGTGATGGTTTTGTTGAAAGTAAGATCAATCTGCTAAACACGGATCAGCAGTCCTTAGATTATCA
TCTGGAGTTAGGAGATAGAATTGTTGCTGTAGATGGAATTCCTGTTATGAGCAATGCAGATATTTTGCGTCTTGTTCAAG
ATCACAGGGTTTCTTTGATTTTTCAGAGGATGTCTTCGGGACAGCTCTCTGTTTTGGATCAAAAAGCTGCAGACAAAGCA
TTTATTGATTCCTATGATATGGACGATCTTCTGCGTGTTGCAGGGTCTGTAGGAGAAGAACGGGAGGTATCTCACTTAGG
AGAGTATCGTCTGGTAACTCGAGTACAGCCTAAGCCATGGGTGCATATTTATTCTGAAGAATTATTAGATAAACAGCGTG
CCCTTGCGTCTAAATTCCGAGATGAGCAAGAGAAGCGCTATTACTTAGAAAGAATAGAATCCGAAAAACAGCGCATTTCT
TTGGGCATTCCCCTTAAGGATCTCGCTGTGCAGTATAATCCAGCTCCCTTAGTTTTGATGGGAGAATCCATTTCTGATAG
CTTAAGGACTGTAAAGGCCTTAGGATCTGGCCGGCTGAGTCCTCAATGGCTTTCAGGGCCTGTGGGAATTGTCCGTATTC
TACATACAGGATGGTCTATGGGGATTCCCGAGGCTCTATCTTGGATCGGATTGATCAGCATAAACCTAGCAGTATTGAAT
CTCCTCCCTATACCGGTTTTGGATGGGGGTTATATACTTTTATGCTTATGGGAGAGCGTTTCTAGACGTCGGTTAAATAT
GCGACTCATTGAAAAAGGACTAGTCCCATTTATGATTCTGCTGATCTTATTTTTTGTGTTCTTGACCCTCCAGGATCTTT
CCAGAGTTTTCATTGGATGA

Upstream 100 bases:

>100_bases
GTCTTGTAGTAACTTAGGTTTCTTTATGGAGGCCTTTTAGCAGCTGTTTTAAGTGATGTTTTAAAGCCGGCTTAGAACGT
TGATTATCCAGGTATAGAGT

Downstream 100 bases:

>100_bases
CATTTTGCTTTTAGGAAATCCATAAAGGCTATAGGGGTGAGCTCCTAAACAGTGAATAATATAGATGTCTTTGCTCTTCT
CTTTTTTCGCTGTAGTGTTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MTVIYFVLAALALGFLILIHELGHLLAAKAVGMTVESFSIGFGPALVRKKMGSIEYRIGAIPFGGYVRIKGMDRNDKEIS
EDREKTVYDIPGGFFSKSPWKRIFVLAAGPLANILVALFAFGILYFSGGRTKPFSEHTSIVGWAHPSLEQKGLRPGDRIF
LCNGQVYSGNKMAFSSSLLDRKLSLQGEHPAYFSEAESFSLDVPFNPSLEGVPCLGASYLLYRGSEPLPEKSPLIDAGLS
EGDRLVWMDGALVFSGVQVSQILNEKKAFLRIERQGKIVFVRQSRVLAGDLQLTSYFKNELIDCQYEAGLKGKWASLYML
PYIINSDGFVESKINLLNTDQQSLDYHLELGDRIVAVDGIPVMSNADILRLVQDHRVSLIFQRMSSGQLSVLDQKAADKA
FIDSYDMDDLLRVAGSVGEEREVSHLGEYRLVTRVQPKPWVHIYSEELLDKQRALASKFRDEQEKRYYLERIESEKQRIS
LGIPLKDLAVQYNPAPLVLMGESISDSLRTVKALGSGRLSPQWLSGPVGIVRILHTGWSMGIPEALSWIGLISINLAVLN
LLPIPVLDGGYILLCLWESVSRRRLNMRLIEKGLVPFMILLILFFVFLTLQDLSRVFIG

Sequences:

>Translated_619_residues
MTVIYFVLAALALGFLILIHELGHLLAAKAVGMTVESFSIGFGPALVRKKMGSIEYRIGAIPFGGYVRIKGMDRNDKEIS
EDREKTVYDIPGGFFSKSPWKRIFVLAAGPLANILVALFAFGILYFSGGRTKPFSEHTSIVGWAHPSLEQKGLRPGDRIF
LCNGQVYSGNKMAFSSSLLDRKLSLQGEHPAYFSEAESFSLDVPFNPSLEGVPCLGASYLLYRGSEPLPEKSPLIDAGLS
EGDRLVWMDGALVFSGVQVSQILNEKKAFLRIERQGKIVFVRQSRVLAGDLQLTSYFKNELIDCQYEAGLKGKWASLYML
PYIINSDGFVESKINLLNTDQQSLDYHLELGDRIVAVDGIPVMSNADILRLVQDHRVSLIFQRMSSGQLSVLDQKAADKA
FIDSYDMDDLLRVAGSVGEEREVSHLGEYRLVTRVQPKPWVHIYSEELLDKQRALASKFRDEQEKRYYLERIESEKQRIS
LGIPLKDLAVQYNPAPLVLMGESISDSLRTVKALGSGRLSPQWLSGPVGIVRILHTGWSMGIPEALSWIGLISINLAVLN
LLPIPVLDGGYILLCLWESVSRRRLNMRLIEKGLVPFMILLILFFVFLTLQDLSRVFIG
>Mature_618_residues
TVIYFVLAALALGFLILIHELGHLLAAKAVGMTVESFSIGFGPALVRKKMGSIEYRIGAIPFGGYVRIKGMDRNDKEISE
DREKTVYDIPGGFFSKSPWKRIFVLAAGPLANILVALFAFGILYFSGGRTKPFSEHTSIVGWAHPSLEQKGLRPGDRIFL
CNGQVYSGNKMAFSSSLLDRKLSLQGEHPAYFSEAESFSLDVPFNPSLEGVPCLGASYLLYRGSEPLPEKSPLIDAGLSE
GDRLVWMDGALVFSGVQVSQILNEKKAFLRIERQGKIVFVRQSRVLAGDLQLTSYFKNELIDCQYEAGLKGKWASLYMLP
YIINSDGFVESKINLLNTDQQSLDYHLELGDRIVAVDGIPVMSNADILRLVQDHRVSLIFQRMSSGQLSVLDQKAADKAF
IDSYDMDDLLRVAGSVGEEREVSHLGEYRLVTRVQPKPWVHIYSEELLDKQRALASKFRDEQEKRYYLERIESEKQRISL
GIPLKDLAVQYNPAPLVLMGESISDSLRTVKALGSGRLSPQWLSGPVGIVRILHTGWSMGIPEALSWIGLISINLAVLNL
LPIPVLDGGYILLCLWESVSRRRLNMRLIEKGLVPFMILLILFFVFLTLQDLSRVFIG

Specific function: Unknown

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M50B family

Homologues:

Organism=Escherichia coli, GI1786373, Length=293, Percent_Identity=31.0580204778157, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y344_CHLMU (Q9PKW7)

Other databases:

- EMBL:   AE002160
- PIR:   H81712
- RefSeq:   NP_296722.1
- ProteinModelPortal:   Q9PKW7
- GeneID:   1246387
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0344
- TIGR:   TC_0344
- HOGENOM:   HBG413936
- OMA:   NELIDNQ
- ProtClustDB:   CLSK871167
- BioCyc:   CMUR243161:TC_0344-MONOMER
- GO:   GO:0006508
- InterPro:   IPR008915

Pfam domain/function: PF02163 Peptidase_M50

EC number: NA

Molecular weight: Translated: 69101; Mature: 68970

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00142 ZINC_PROTEASE; PS00136 SUBTILASE_ASP

Important sites: ACT_SITE 21-21

Signals:

None

Transmembrane regions:

HASH(0xb9e2a00)-; HASH(0xc2b7744)-; HASH(0xbcb76dc)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIYFVLAALALGFLILIHELGHLLAAKAVGMTVESFSIGFGPALVRKKMGSIEYRIGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCEEEEEE
IPFGGYVRIKGMDRNDKEISEDREKTVYDIPGGFFSKSPWKRIFVLAAGPLANILVALFA
ECCCCEEEEEECCCCCHHHHHHHHCEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
FGILYFSGGRTKPFSEHTSIVGWAHPSLEQKGLRPGDRIFLCNGQVYSGNKMAFSSSLLD
HHHHHCCCCCCCCCCCCCCEEEECCCCHHHCCCCCCCEEEEECCEEECCCCHHHHHHHHH
RKLSLQGEHPAYFSEAESFSLDVPFNPSLEGVPCLGASYLLYRGSEPLPEKSPLIDAGLS
HHHCCCCCCCCHHCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
EGDRLVWMDGALVFSGVQVSQILNEKKAFLRIERQGKIVFVRQSRVLAGDLQLTSYFKNE
CCCEEEEECCHHEECCCHHHHHHHHHHHHEEEECCCCEEEEECCCEEECHHHHHHHHHHH
LIDCQYEAGLKGKWASLYMLPYIINSDGFVESKINLLNTDQQSLDYHLELGDRIVAVDGI
CCCCEECCCCCCCCEEEEEEEEEECCCCCEECCHHEECCCHHHCEEEEECCCEEEEECCC
PVMSNADILRLVQDHRVSLIFQRMSSGQLSVLDQKAADKAFIDSYDMDDLLRVAGSVGEE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHCCCCHHHHHHHHCCCCCC
REVSHLGEYRLVTRVQPKPWVHIYSEELLDKQRALASKFRDEQEKRYYLERIESEKQRIS
HHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEE
LGIPLKDLAVQYNPAPLVLMGESISDSLRTVKALGSGRLSPQWLSGPVGIVRILHTGWSM
ECCCHHHHEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCCCC
GIPEALSWIGLISINLAVLNLLPIPVLDGGYILLCLWESVSRRRLNMRLIEKGLVPFMIL
CHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEHHHHHHHHHHHHHHHHCHHHHHHH
LILFFVFLTLQDLSRVFIG
HHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
TVIYFVLAALALGFLILIHELGHLLAAKAVGMTVESFSIGFGPALVRKKMGSIEYRIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCEEEEEE
IPFGGYVRIKGMDRNDKEISEDREKTVYDIPGGFFSKSPWKRIFVLAAGPLANILVALFA
ECCCCEEEEEECCCCCHHHHHHHHCEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
FGILYFSGGRTKPFSEHTSIVGWAHPSLEQKGLRPGDRIFLCNGQVYSGNKMAFSSSLLD
HHHHHCCCCCCCCCCCCCCEEEECCCCHHHCCCCCCCEEEEECCEEECCCCHHHHHHHHH
RKLSLQGEHPAYFSEAESFSLDVPFNPSLEGVPCLGASYLLYRGSEPLPEKSPLIDAGLS
HHHCCCCCCCCHHCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
EGDRLVWMDGALVFSGVQVSQILNEKKAFLRIERQGKIVFVRQSRVLAGDLQLTSYFKNE
CCCEEEEECCHHEECCCHHHHHHHHHHHHEEEECCCCEEEEECCCEEECHHHHHHHHHHH
LIDCQYEAGLKGKWASLYMLPYIINSDGFVESKINLLNTDQQSLDYHLELGDRIVAVDGI
CCCCEECCCCCCCCEEEEEEEEEECCCCCEECCHHEECCCHHHCEEEEECCCEEEEECCC
PVMSNADILRLVQDHRVSLIFQRMSSGQLSVLDQKAADKAFIDSYDMDDLLRVAGSVGEE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHCCCCHHHHHHHHCCCCCC
REVSHLGEYRLVTRVQPKPWVHIYSEELLDKQRALASKFRDEQEKRYYLERIESEKQRIS
HHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEE
LGIPLKDLAVQYNPAPLVLMGESISDSLRTVKALGSGRLSPQWLSGPVGIVRILHTGWSM
ECCCHHHHEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHCCCCC
GIPEALSWIGLISINLAVLNLLPIPVLDGGYILLCLWESVSRRRLNMRLIEKGLVPFMIL
CHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEHHHHHHHHHHHHHHHHCHHHHHHH
LILFFVFLTLQDLSRVFIG
HHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10684935