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The map label for this gene is lepA

Identifier: 15834954

GI number: 15834954

Start: 395815

End: 397623

Strand: Reverse

Name: lepA

Synonym: TC0334

Alternate gene names: 15834954

Gene position: 397623-395815 (Counterclockwise)

Preceding gene: 15834955

Following gene: 15834949

Centisome position: 37.06

GC content: 39.08

Gene sequence:

>1809_bases
TTGAAACCGTATAAGATTGAAAACATTCGTAATTTTTCTATTATTGCCCATATTGACCACGGTAAGTCCACTATCGCAGA
TCGGTTGCTAGAGAGCACTAGCACGATTGAACAACGAGAAATGCGTGAACAGCTTTTAGACTCTATGGACTTGGAAAGAG
AGCGTGGTATTACCATTAAGGCGCATCCAGTCACAATGACTTATGAATACGAGGGGGAAACTTACGAACTCAATCTCATA
GATACTCCAGGACACGTGGATTTTTCTTATGAAGTTTCTCGATCCCTGGCAGCATGTGAAGGAGCTTTGCTTATAGTCGA
CGCGGCACAAGGTGTGCAAGCGCAAAGCTTAGCTAATGTATACCTAGCGCTAGAACGAGATTTAGAAATCATTCCAGTTT
TAAATAAAATAGATCTTCCTGCAGCTCAACCAGAGGCTATAAAAAAACAAATCGAAGAATTCATTGGACTAGATACATCT
AATGCTATTGCTTGCTCAGCGAAAACAGGTCAAGGCATCCCAGAAATTTTAGAGTCTATTATACGGCTCGTGCCTCCCCC
AAAACCATCTCAAGAAACAGAACTCAAAGCATTAATCTTTGATTCTCACTATGATCCTTATGTAGGAATCATGGTATATG
TGCGAGTGATTAGCGGAGAAATCAAAAAAGGTGATCGTATTACCTTTATGTCAACCAAGGGCTCTTCTTTTGAAGTTTTA
GGGATTGGGGCCTTCTTACCGGAAGCTACCTTAATAGAAGGGTCTTTACGAGCTGGACAAGTAGGATATTTTATCGCTAA
CCTGAAAAAGGTTAAGGATGTAAAAATTGGCGATACAGTTACTACAGTTAAGCATCCCGCGAAAGAGCCTTTAGAAGGAT
TCAAGGAAATTAAACCTGTTGTTTTTGCAGGAATCTATCCCATAGACTCTTCTGATTTTGATACCCTTAAAGATGCTTTA
GGCCGTTTACAACTTAACGACTCAGCTCTTACGATTGAGCAAGAGAGTAGCCATTCCCTTGGGTTTGGGTTCCGTTGTGG
ATTTTTAGGCCTTCTTCATTTAGAAATTATCTTTGAAAGAATTTCGAGAGAATTTGATCTTGATATTATCGCTACTGCCC
CAAGCGTTATTTACAAAGTAGTCTTAAAAAATGGGAAAACTCTTTTTATTGATAATCCGACGGCATATCCAGACCCGGCT
CTTATTGAGCATATGGAAGAGCCCTGGGTTCATGTAAATATCATTACTCCCCAAGAGTATCTAAGCAACATCATGAGCCT
ATGTATGGACAAGCGGGGAATATGCTTGAAAACGGATATGCTTGACCAGCACAGACTCGTCCTTTCCTATGAGCTTCCTC
TTAATGAAATTGTTTCTGATTTTAATGATAAACTTAAATCCGTTACAAAAGGATATGGCTCTTTCGACTATCGTCTAGGA
GATTACAAACCTGGCGCCATCATTAAACTAGAAATTCTTATCAATGACGAAACTGTAGACGCTTTTTCTTGTCTTGTACA
TAGGGATAAAGCGGAATCAAAAGGACGAAGCATTTGTGAAAAACTTGTAGATGTTATTCCTCCTCAACTCTTTAAAATTC
CTATTCAAGCTGCTATCAATAAAAAAATTATTGCTAGAGAAACGATTCGCGCTTTAGCGAAAAACGTTACTGCAAAATGC
TATGGAGGAGACATCACGAGAAAACGTAAGTTATGGGAAAAACAGAAAAAGGGTAAAAAACGAATGAAAGAGTTTGGGAA
AGTGTCTATTCCCAACACAGCATTTGTTGAAGTCCTTAAGATGGAATAA

Upstream 100 bases:

>100_bases
TCTTCACAAATCTCTTCTAATCTCCTACTATTTTTCTCTAGATAGACAACTATCAATTCCGTTAACATATCCTTTTCAAA
GCAGAAATTCGGTCACAAAC

Downstream 100 bases:

>100_bases
GGGCAAGTTTTCTCAAAAATTCTTCCATAAAAAAGCCCGGCAAATACTTTTGCCGGGCTTTATCTTTTTACATCCGGGAA
GCGTTTTTGCTTCCTAACCA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MKPYKIENIRNFSIIAHIDHGKSTIADRLLESTSTIEQREMREQLLDSMDLERERGITIKAHPVTMTYEYEGETYELNLI
DTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPVLNKIDLPAAQPEAIKKQIEEFIGLDTS
NAIACSAKTGQGIPEILESIIRLVPPPKPSQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMSTKGSSFEVL
GIGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKEPLEGFKEIKPVVFAGIYPIDSSDFDTLKDAL
GRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFERISREFDLDIIATAPSVIYKVVLKNGKTLFIDNPTAYPDPA
LIEHMEEPWVHVNIITPQEYLSNIMSLCMDKRGICLKTDMLDQHRLVLSYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYKPGAIIKLEILINDETVDAFSCLVHRDKAESKGRSICEKLVDVIPPQLFKIPIQAAINKKIIARETIRALAKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFVEVLKME

Sequences:

>Translated_602_residues
MKPYKIENIRNFSIIAHIDHGKSTIADRLLESTSTIEQREMREQLLDSMDLERERGITIKAHPVTMTYEYEGETYELNLI
DTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPVLNKIDLPAAQPEAIKKQIEEFIGLDTS
NAIACSAKTGQGIPEILESIIRLVPPPKPSQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMSTKGSSFEVL
GIGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKEPLEGFKEIKPVVFAGIYPIDSSDFDTLKDAL
GRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFERISREFDLDIIATAPSVIYKVVLKNGKTLFIDNPTAYPDPA
LIEHMEEPWVHVNIITPQEYLSNIMSLCMDKRGICLKTDMLDQHRLVLSYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYKPGAIIKLEILINDETVDAFSCLVHRDKAESKGRSICEKLVDVIPPQLFKIPIQAAINKKIIARETIRALAKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFVEVLKME
>Mature_602_residues
MKPYKIENIRNFSIIAHIDHGKSTIADRLLESTSTIEQREMREQLLDSMDLERERGITIKAHPVTMTYEYEGETYELNLI
DTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPVLNKIDLPAAQPEAIKKQIEEFIGLDTS
NAIACSAKTGQGIPEILESIIRLVPPPKPSQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMSTKGSSFEVL
GIGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKEPLEGFKEIKPVVFAGIYPIDSSDFDTLKDAL
GRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFERISREFDLDIIATAPSVIYKVVLKNGKTLFIDNPTAYPDPA
LIEHMEEPWVHVNIITPQEYLSNIMSLCMDKRGICLKTDMLDQHRLVLSYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYKPGAIIKLEILINDETVDAFSCLVHRDKAESKGRSICEKLVDVIPPQLFKIPIQAAINKKIIARETIRALAKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFVEVLKME

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=610, Percent_Identity=49.1803278688525, Blast_Score=611, Evalue=1e-175,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=43.1818181818182, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI4503483, Length=147, Percent_Identity=38.0952380952381, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI25306283, Length=140, Percent_Identity=42.8571428571429, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI19923640, Length=140, Percent_Identity=42.8571428571429, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI25306287, Length=140, Percent_Identity=42.8571428571429, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI18390331, Length=151, Percent_Identity=36.4238410596026, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=42.2018348623853, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=42.2018348623853, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=42.2018348623853, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI217272894, Length=136, Percent_Identity=38.9705882352941, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI217272892, Length=136, Percent_Identity=38.9705882352941, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI94966752, Length=84, Percent_Identity=41.6666666666667, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=51.1705685618729, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=514, Percent_Identity=25.875486381323, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=36.4779874213836, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790835, Length=161, Percent_Identity=31.055900621118, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789559, Length=290, Percent_Identity=26.2068965517241, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=614, Percent_Identity=39.7394136807818, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17556745, Length=461, Percent_Identity=26.0303687635575, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=40.4109589041096, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=35.2564102564103, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=38.3458646616541, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=38.3458646616541, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=34.5864661654135, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=154, Percent_Identity=31.1688311688312, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566303, Length=311, Percent_Identity=26.6881028938907, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=43.046357615894, Blast_Score=495, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6323098, Length=146, Percent_Identity=39.041095890411, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=38.7755102040816, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=38.7755102040816, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=38.5964912280702, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=39.7163120567376, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=281, Percent_Identity=23.8434163701068, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319282, Length=276, Percent_Identity=25, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=43.4494195688226, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24582462, Length=152, Percent_Identity=40.7894736842105, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=42.7536231884058, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=37.7483443708609, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=37.7483443708609, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=37.7483443708609, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=37.7777777777778, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221458488, Length=137, Percent_Identity=37.956204379562, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281363316, Length=290, Percent_Identity=26.2068965517241, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864358, Length=290, Percent_Identity=26.2068965517241, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67380; Mature: 67380

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYKIENIRNFSIIAHIDHGKSTIADRLLESTSTIEQREMREQLLDSMDLERERGITIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEE
AHPVTMTYEYEGETYELNLIDTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANV
EEEEEEEEEECCCEEEEEEEECCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
YLALERDLEIIPVLNKIDLPAAQPEAIKKQIEEFIGLDTSNAIACSAKTGQGIPEILESI
HEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
IRLVPPPKPSQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMSTKGSSFEVL
HHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEE
GIGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKEPLEGFKEIKPV
EEHHHCCCCCEEECCCCCCCHHHHHHHHHHHCEEECCCCCHHCCCCCHHHHHHHHHCCCE
VFAGIYPIDSSDFDTLKDALGRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFER
EEEEEECCCCCCHHHHHHHHCCEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
ISREFDLDIIATAPSVIYKVVLKNGKTLFIDNPTAYPDPALIEHMEEPWVHVNIITPQEY
HCCCCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEEECHHHH
LSNIMSLCMDKRGICLKTDMLDQHRLVLSYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
HHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECC
DYKPGAIIKLEILINDETVDAFSCLVHRDKAESKGRSICEKLVDVIPPQLFKIPIQAAIN
CCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHEEECHHHHHC
KKIIARETIRALAKNVTAKCYGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFVEVLK
HHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEE
ME
CC
>Mature Secondary Structure
MKPYKIENIRNFSIIAHIDHGKSTIADRLLESTSTIEQREMREQLLDSMDLERERGITIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEE
AHPVTMTYEYEGETYELNLIDTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANV
EEEEEEEEEECCCEEEEEEEECCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
YLALERDLEIIPVLNKIDLPAAQPEAIKKQIEEFIGLDTSNAIACSAKTGQGIPEILESI
HEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
IRLVPPPKPSQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMSTKGSSFEVL
HHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEE
GIGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKEPLEGFKEIKPV
EEHHHCCCCCEEECCCCCCCHHHHHHHHHHHCEEECCCCCHHCCCCCHHHHHHHHHCCCE
VFAGIYPIDSSDFDTLKDALGRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFER
EEEEEECCCCCCHHHHHHHHCCEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
ISREFDLDIIATAPSVIYKVVLKNGKTLFIDNPTAYPDPALIEHMEEPWVHVNIITPQEY
HCCCCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEEECHHHH
LSNIMSLCMDKRGICLKTDMLDQHRLVLSYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
HHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECC
DYKPGAIIKLEILINDETVDAFSCLVHRDKAESKGRSICEKLVDVIPPQLFKIPIQAAIN
CCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHEEECHHHHHC
KKIIARETIRALAKNVTAKCYGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFVEVLK
HHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEE
ME
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA