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The map label for this gene is tyrS

Identifier: 15834952

GI number: 15834952

Start: 393060

End: 394298

Strand: Direct

Name: tyrS

Synonym: TC0332

Alternate gene names: 15834952

Gene position: 393060-394298 (Clockwise)

Preceding gene: 15834951

Following gene: 15834953

Centisome position: 36.63

GC content: 40.92

Gene sequence:

>1239_bases
ATGCAACAATTAATCGATAGCCTTCAGAAACGGGGTATTCTAGATAATTCTTCCGCAGGATTAGAAAGTTTAACAGCTCC
TGTTTCTGCCTATTTGGGGTTCGATCCAACTGCTCCTTCCTTGCATATAGGGCATTGGATTGGAATTTGTTTTCTGCGTC
GATTATCGGCATATGGAATCACTCCTATTGCTCTTGTTGGAGGAGCAACAGGGATGATAGGGGATCCTTCTGGTAAAAGT
GTGGAGCGCTCGCTATTAGATCAAGAGCAAGTACTTGATAATAGCAAAAAGATTGAGGTGGCTCTTGCTAATTATCTTCC
CGACATTCGCATTGTGAACAATGCCGATTGGTTGGGATCATTGAGTATGGTGGATTTTCTAAGAGATATTGGGAAGTATT
TCCGCTTAGGCTCGATGTTAGCTAAGGACGTAGTGAAACAGCGAGTTTATTCTGAAGAAGGAATCAGTTATACAGAGTTT
AGTTATTTGTTGCTGCAATCCTATGATTTTGCTCATCTTTTTAAGCACCATGGTGTTGTATTGCAGTGTGGAGGAAGTGA
CCAGTGGGGAAACATTACCTCTGGAATTGATTATATTCGTCGGAAGGGGTTAGGACAAGCTTTTGGACTCACCTATCCTT
TGTTGACTGATAGCAAAGGGAAAAAAATTGGGAAAACGGAGTCGGGAACTGTTTGGTTAGATCCTGAATTAACGTCTCCT
TATGAATTATTTCAGTATTTCTTACGTTTATCGGATCAAGAAATCCCTAAAATAGCGAGGATGCTCACTTTACTTGATGA
TGATGAGGTTCTTGCTCTTGATAAGCGGTTGGAGAATGATCCGCAAGCAGTAAAAAGGTATGTGGCAGAAGTCATTGTTA
AGGATGTGCATGGTGCTGAGGGATTAGCACAGGCACTGGCTACTACAGAGAGCTTTTTTGCTAATAAAGGAAAAAATATT
ACCGAATCGGAACTCGCGGCGTTAGTTCAGTCTGGTGTTGGTATCAATGTAGCTCGAGCAGATGTTATAGGGAAACGCTG
GTTAGATGTCGTTGTGCAATTGGGATTTTGTTCTTCAAAAGGGGAAGCTAGAAGACTCATTCAGCAGCGAGGTTTATATG
TGAATCAGGAACCTTTAATAGATGAACAGAGTGTCCTAGATGGGACATATCTCTGTTTTGATCGGTATATTCTTCTCTCT
CAAGGGAAAAAGAAAAAACAAGTTATAGATCTTAATTAG

Upstream 100 bases:

>100_bases
GACCTATTTTGTTAGCAGTATATCTGGAACAGCTTGCCTTGCTATAAAAGGAACAGGATAGATAAGATGTTGCTAGATAA
GTTTATATGGACAGATTTTT

Downstream 100 bases:

>100_bases
GAGGTGGCAGTGGCTCCAGCAGATATTGGGTTGATTGGTTTGGCCGTAATGGGCAAAAACCTTGTGTTGAATATGATTGA
TCACGGTTTTGCTGTCTCTG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQQLIDSLQKRGILDNSSAGLESLTAPVSAYLGFDPTAPSLHIGHWIGICFLRRLSAYGITPIALVGGATGMIGDPSGKS
VERSLLDQEQVLDNSKKIEVALANYLPDIRIVNNADWLGSLSMVDFLRDIGKYFRLGSMLAKDVVKQRVYSEEGISYTEF
SYLLLQSYDFAHLFKHHGVVLQCGGSDQWGNITSGIDYIRRKGLGQAFGLTYPLLTDSKGKKIGKTESGTVWLDPELTSP
YELFQYFLRLSDQEIPKIARMLTLLDDDEVLALDKRLENDPQAVKRYVAEVIVKDVHGAEGLAQALATTESFFANKGKNI
TESELAALVQSGVGINVARADVIGKRWLDVVVQLGFCSSKGEARRLIQQRGLYVNQEPLIDEQSVLDGTYLCFDRYILLS
QGKKKKQVIDLN

Sequences:

>Translated_412_residues
MQQLIDSLQKRGILDNSSAGLESLTAPVSAYLGFDPTAPSLHIGHWIGICFLRRLSAYGITPIALVGGATGMIGDPSGKS
VERSLLDQEQVLDNSKKIEVALANYLPDIRIVNNADWLGSLSMVDFLRDIGKYFRLGSMLAKDVVKQRVYSEEGISYTEF
SYLLLQSYDFAHLFKHHGVVLQCGGSDQWGNITSGIDYIRRKGLGQAFGLTYPLLTDSKGKKIGKTESGTVWLDPELTSP
YELFQYFLRLSDQEIPKIARMLTLLDDDEVLALDKRLENDPQAVKRYVAEVIVKDVHGAEGLAQALATTESFFANKGKNI
TESELAALVQSGVGINVARADVIGKRWLDVVVQLGFCSSKGEARRLIQQRGLYVNQEPLIDEQSVLDGTYLCFDRYILLS
QGKKKKQVIDLN
>Mature_412_residues
MQQLIDSLQKRGILDNSSAGLESLTAPVSAYLGFDPTAPSLHIGHWIGICFLRRLSAYGITPIALVGGATGMIGDPSGKS
VERSLLDQEQVLDNSKKIEVALANYLPDIRIVNNADWLGSLSMVDFLRDIGKYFRLGSMLAKDVVKQRVYSEEGISYTEF
SYLLLQSYDFAHLFKHHGVVLQCGGSDQWGNITSGIDYIRRKGLGQAFGLTYPLLTDSKGKKIGKTESGTVWLDPELTSP
YELFQYFLRLSDQEIPKIARMLTLLDDDEVLALDKRLENDPQAVKRYVAEVIVKDVHGAEGLAQALATTESFFANKGKNI
TESELAALVQSGVGINVARADVIGKRWLDVVVQLGFCSSKGEARRLIQQRGLYVNQEPLIDEQSVLDGTYLCFDRYILLS
QGKKKKQVIDLN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=403, Percent_Identity=34.4913151364764, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=41.527446300716, Blast_Score=309, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI25145802, Length=380, Percent_Identity=36.8421052631579, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI25145805, Length=275, Percent_Identity=36, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6325160, Length=443, Percent_Identity=30.2483069977427, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI20130383, Length=405, Percent_Identity=36.7901234567901, Blast_Score=251, Evalue=4e-67,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CHLMU (Q9PKX8)

Other databases:

- EMBL:   AE002160
- PIR:   H81713
- RefSeq:   NP_296711.1
- ProteinModelPortal:   Q9PKX8
- SMR:   Q9PKX8
- GeneID:   1246376
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0332
- TIGR:   TC_0332
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q9PKX8
- ProtClustDB:   PRK05912
- BioCyc:   CMUR243161:TC_0332-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45631; Mature: 45631

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 31-31 BINDING 162-162 BINDING 166-166 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLIDSLQKRGILDNSSAGLESLTAPVSAYLGFDPTAPSLHIGHWIGICFLRRLSAYGI
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCC
TPIALVGGATGMIGDPSGKSVERSLLDQEQVLDNSKKIEVALANYLPDIRIVNNADWLGS
CHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCEEEECCCCHHHH
LSMVDFLRDIGKYFRLGSMLAKDVVKQRVYSEEGISYTEFSYLLLQSYDFAHLFKHHGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCEE
LQCGGSDQWGNITSGIDYIRRKGLGQAFGLTYPLLTDSKGKKIGKTESGTVWLDPELTSP
EEECCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCCCCEEEECCCCCCH
YELFQYFLRLSDQEIPKIARMLTLLDDDEVLALDKRLENDPQAVKRYVAEVIVKDVHGAE
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHH
GLAQALATTESFFANKGKNITESELAALVQSGVGINVARADVIGKRWLDVVVQLGFCSSK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCC
GEARRLIQQRGLYVNQEPLIDEQSVLDGTYLCFDRYILLSQGKKKKQVIDLN
HHHHHHHHHCCCEECCCCCCCCHHHHCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQLIDSLQKRGILDNSSAGLESLTAPVSAYLGFDPTAPSLHIGHWIGICFLRRLSAYGI
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCC
TPIALVGGATGMIGDPSGKSVERSLLDQEQVLDNSKKIEVALANYLPDIRIVNNADWLGS
CHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCEEEECCCCHHHH
LSMVDFLRDIGKYFRLGSMLAKDVVKQRVYSEEGISYTEFSYLLLQSYDFAHLFKHHGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCEE
LQCGGSDQWGNITSGIDYIRRKGLGQAFGLTYPLLTDSKGKKIGKTESGTVWLDPELTSP
EEECCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCCCCEEEECCCCCCH
YELFQYFLRLSDQEIPKIARMLTLLDDDEVLALDKRLENDPQAVKRYVAEVIVKDVHGAE
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHH
GLAQALATTESFFANKGKNITESELAALVQSGVGINVARADVIGKRWLDVVVQLGFCSSK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCC
GEARRLIQQRGLYVNQEPLIDEQSVLDGTYLCFDRYILLSQGKKKKQVIDLN
HHHHHHHHHCCCEECCCCCCCCHHHHCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935