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Identifier: 15834950

GI number: 15834950

Start: 390297

End: 392033

Strand: Direct

Name: Not Available

Synonym: TC0330

Alternate gene names: 15834950

Gene position: 390297-392033 (Clockwise)

Preceding gene: 15834945

Following gene: 15834951

Centisome position: 36.38

GC content: 38.98

Gene sequence:

>1737_bases
GTGAATCACATTAGAGGAAGTTCATGGAAAATTGTAACAATCCCCATTTGTATATTATTCACTTTAGTCATCCCTCTACC
AAGATGGATTATTGATTTTGGAGTATGTGCAAATCTAGCCTGTTCTTTATCGATCATTTTTTGGGTGTTCTCTTTACGAT
CTTCTGCTTCTGCTAGAATTTTCCCTTCACTCCTTCTATATCTTTGTTTATTACGTCTTGGACTCAATTTAGCTTCCACG
CGATGGATTTTAGCTTCTGGATGGGCATCTCCTTTGATTTTTGCTCTAGGAAGTTTCTTTTCTTTGGGGAGTATCCCCGT
TGCACTAACAGTATGTTTACTACTATTTTTCATAAATTTTCTTGTTATCACCAAAGGAGCAGAGCGAATTGCAGAGGTAC
GGGCGCGTTTTTCTTTAGAATCGCTTCCAGGGAAGCAGATGTCTTTGGATGCCGATATTTCTGCAGGAAGAATTGGCTCT
AGTAGAGCTTCTGTCAAAAAGAACTCTCTTTTGGAAGAGAGTGATTATTTTTCAGCTATGGAGGGAGTTTTTCGCTTCGT
AAAAGGTGATGCAATAATGAGTTGGGTGCTGTTAGGAGTGAATAGTCTGGCAGCTTTATTTTTGGGACGTCATGTTGGGG
TTTATAATTTATGGTTAACTGTGTTGGGCGATGCCTTAGTCAGTCAAGTTCCTGCATTGCTAACATCTTGTTCAGCAGCC
ACCCTTATAGCAAAAGTTGGAGAAAAAGAGAGCTTAGCGCAACATCTTGTGGAGTATTATGAGCAATGTAGACAAAGTTT
TTTATATATCGCATTGATTCTTTGTGGAATGGCTTTTATTCCAGGGGCTCCTAAAGCTCTTATTTTAGGATTTTCTGTTT
TGTTACTGTTAGGGTACAAGAGCCCCTATTCAGAAGAATCTTTATTATTCCAGAAGGAGCGGATAGAGCTATTATTACCC
AATAAAGGAAATGAAAATCCTGTAAGTTTGTATAGGGCTGCTCGTCATCAAATTTATCAAGAATTAGGGGTAATCTTCCC
GGAAGAGAGTGTGGTGCGTTATGTAAATAATGTTTCTCCGAGATTGGTATTTTCTGGGCAGGAGATCTCTTTAACAGAGC
TTTCTTGCTCTGCCATGTTAAAGGCTATGAGGAAGCTAGCTCCCGAGACGATAGGTGAACGTTTTATTGCTCGTCTTATT
GAGGAATTTCAAGAGCAGGGGGGGCTGTCTATAGAGGAGATCATTCCTCTTAAAATATCCGAAAACTCTTTAGTTTTCTT
ATTGAGAGCTCTTGTTAAAGAGCAGGTCTCTTTACATTTATTTCCTAAAATTCTTGAAGCTATTGATTTATTTGGCTCCC
AGGCAAAAAGTTCTCTGGAGTTAGTAGAGTCTGTGCGCAACTACTTAGGGAAACAAATAGGTTTATCTTTATGGAACAGA
AAAGATGTTCTAGAAGTAATTACCGTAGATTCTCTGGTTGAGCAATTTGTGCGAGATTCGCAAGCGAAGGTTTTTGTAGA
TTTGAACGAAAAAGTTGTTTCTCAAGTGAAAGACTTATTACGGGAAGGGGAGGGAAATTTTCGAGCTATTGTCACAGGAT
CTGAAACAAGAAAAGAACTAAAGCGCATAGTGGATCCTTATTTCCCAGATTTACTCGTTTTAGCTCATAGCGAGCTTCCT
GAAGAGATGCCTATAACCTTGCTAGGAGCTGTTTCTGATGAGGTTTTATTATCTTAA

Upstream 100 bases:

>100_bases
AAGATAAAGATGTTAAAAATGATAAATATTTCTGAGAACTTCTTCTTGAAGATATATGTTTTTGCTGGGTATTCGTTCGA
TGTAGAATTGGGGGGCTATC

Downstream 100 bases:

>100_bases
AAAGGTTGTTTGTTGGGAGTTATTTGTTACTTGTGAAAATGTAGACATTGTTTAAAATTTTTATTTGATTTAATCTTATT
TTTTTAGTCTGTGAAAGAAT

Product: type III secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MNHIRGSSWKIVTIPICILFTLVIPLPRWIIDFGVCANLACSLSIIFWVFSLRSSASARIFPSLLLYLCLLRLGLNLAST
RWILASGWASPLIFALGSFFSLGSIPVALTVCLLLFFINFLVITKGAERIAEVRARFSLESLPGKQMSLDADISAGRIGS
SRASVKKNSLLEESDYFSAMEGVFRFVKGDAIMSWVLLGVNSLAALFLGRHVGVYNLWLTVLGDALVSQVPALLTSCSAA
TLIAKVGEKESLAQHLVEYYEQCRQSFLYIALILCGMAFIPGAPKALILGFSVLLLLGYKSPYSEESLLFQKERIELLLP
NKGNENPVSLYRAARHQIYQELGVIFPEESVVRYVNNVSPRLVFSGQEISLTELSCSAMLKAMRKLAPETIGERFIARLI
EEFQEQGGLSIEEIIPLKISENSLVFLLRALVKEQVSLHLFPKILEAIDLFGSQAKSSLELVESVRNYLGKQIGLSLWNR
KDVLEVITVDSLVEQFVRDSQAKVFVDLNEKVVSQVKDLLREGEGNFRAIVTGSETRKELKRIVDPYFPDLLVLAHSELP
EEMPITLLGAVSDEVLLS

Sequences:

>Translated_578_residues
MNHIRGSSWKIVTIPICILFTLVIPLPRWIIDFGVCANLACSLSIIFWVFSLRSSASARIFPSLLLYLCLLRLGLNLAST
RWILASGWASPLIFALGSFFSLGSIPVALTVCLLLFFINFLVITKGAERIAEVRARFSLESLPGKQMSLDADISAGRIGS
SRASVKKNSLLEESDYFSAMEGVFRFVKGDAIMSWVLLGVNSLAALFLGRHVGVYNLWLTVLGDALVSQVPALLTSCSAA
TLIAKVGEKESLAQHLVEYYEQCRQSFLYIALILCGMAFIPGAPKALILGFSVLLLLGYKSPYSEESLLFQKERIELLLP
NKGNENPVSLYRAARHQIYQELGVIFPEESVVRYVNNVSPRLVFSGQEISLTELSCSAMLKAMRKLAPETIGERFIARLI
EEFQEQGGLSIEEIIPLKISENSLVFLLRALVKEQVSLHLFPKILEAIDLFGSQAKSSLELVESVRNYLGKQIGLSLWNR
KDVLEVITVDSLVEQFVRDSQAKVFVDLNEKVVSQVKDLLREGEGNFRAIVTGSETRKELKRIVDPYFPDLLVLAHSELP
EEMPITLLGAVSDEVLLS
>Mature_578_residues
MNHIRGSSWKIVTIPICILFTLVIPLPRWIIDFGVCANLACSLSIIFWVFSLRSSASARIFPSLLLYLCLLRLGLNLAST
RWILASGWASPLIFALGSFFSLGSIPVALTVCLLLFFINFLVITKGAERIAEVRARFSLESLPGKQMSLDADISAGRIGS
SRASVKKNSLLEESDYFSAMEGVFRFVKGDAIMSWVLLGVNSLAALFLGRHVGVYNLWLTVLGDALVSQVPALLTSCSAA
TLIAKVGEKESLAQHLVEYYEQCRQSFLYIALILCGMAFIPGAPKALILGFSVLLLLGYKSPYSEESLLFQKERIELLLP
NKGNENPVSLYRAARHQIYQELGVIFPEESVVRYVNNVSPRLVFSGQEISLTELSCSAMLKAMRKLAPETIGERFIARLI
EEFQEQGGLSIEEIIPLKISENSLVFLLRALVKEQVSLHLFPKILEAIDLFGSQAKSSLELVESVRNYLGKQIGLSLWNR
KDVLEVITVDSLVEQFVRDSQAKVFVDLNEKVVSQVKDLLREGEGNFRAIVTGSETRKELKRIVDPYFPDLLVLAHSELP
EEMPITLLGAVSDEVLLS

Specific function: Required for formation of the rod structure of the flagellar apparatus. Together with fliI and fliH, may constitute the export apparatus of flagellin [H]

COG id: COG1298

COG function: function code NU; Flagellar biosynthesis pathway, component FlhA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FHIPEP (flagella/HR/invasion proteins export pore) family [H]

Homologues:

Organism=Escherichia coli, GI1788187, Length=310, Percent_Identity=35.1612903225806, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006301
- InterPro:   IPR001712 [H]

Pfam domain/function: PF00771 FHIPEP [H]

EC number: NA

Molecular weight: Translated: 64155; Mature: 64155

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHIRGSSWKIVTIPICILFTLVIPLPRWIIDFGVCANLACSLSIIFWVFSLRSSASARI
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FPSLLLYLCLLRLGLNLASTRWILASGWASPLIFALGSFFSLGSIPVALTVCLLLFFINF
HHHHHHHHHHHHHCCCHHHCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVITKGAERIAEVRARFSLESLPGKQMSLDADISAGRIGSSRASVKKNSLLEESDYFSAM
HHHHCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EGVFRFVKGDAIMSWVLLGVNSLAALFLGRHVGVYNLWLTVLGDALVSQVPALLTSCSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIAKVGEKESLAQHLVEYYEQCRQSFLYIALILCGMAFIPGAPKALILGFSVLLLLGYK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
SPYSEESLLFQKERIELLLPNKGNENPVSLYRAARHQIYQELGVIFPEESVVRYVNNVSP
CCCCCHHHHHHHHCHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
RLVFSGQEISLTELSCSAMLKAMRKLAPETIGERFIARLIEEFQEQGGLSIEEIIPLKIS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECEEEC
ENSLVFLLRALVKEQVSLHLFPKILEAIDLFGSQAKSSLELVESVRNYLGKQIGLSLWNR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KDVLEVITVDSLVEQFVRDSQAKVFVDLNEKVVSQVKDLLREGEGNFRAIVTGSETRKEL
HHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHH
KRIVDPYFPDLLVLAHSELPEEMPITLLGAVSDEVLLS
HHHHCCCCCHHHHHHHHCCCCCCCEEHEECCCHHHHCC
>Mature Secondary Structure
MNHIRGSSWKIVTIPICILFTLVIPLPRWIIDFGVCANLACSLSIIFWVFSLRSSASARI
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FPSLLLYLCLLRLGLNLASTRWILASGWASPLIFALGSFFSLGSIPVALTVCLLLFFINF
HHHHHHHHHHHHHCCCHHHCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVITKGAERIAEVRARFSLESLPGKQMSLDADISAGRIGSSRASVKKNSLLEESDYFSAM
HHHHCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EGVFRFVKGDAIMSWVLLGVNSLAALFLGRHVGVYNLWLTVLGDALVSQVPALLTSCSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLIAKVGEKESLAQHLVEYYEQCRQSFLYIALILCGMAFIPGAPKALILGFSVLLLLGYK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
SPYSEESLLFQKERIELLLPNKGNENPVSLYRAARHQIYQELGVIFPEESVVRYVNNVSP
CCCCCHHHHHHHHCHHEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
RLVFSGQEISLTELSCSAMLKAMRKLAPETIGERFIARLIEEFQEQGGLSIEEIIPLKIS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECEEEC
ENSLVFLLRALVKEQVSLHLFPKILEAIDLFGSQAKSSLELVESVRNYLGKQIGLSLWNR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KDVLEVITVDSLVEQFVRDSQAKVFVDLNEKVVSQVKDLLREGEGNFRAIVTGSETRKEL
HHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHH
KRIVDPYFPDLLVLAHSELPEEMPITLLGAVSDEVLLS
HHHHCCCCCHHHHHHHHCCCCCCCEEHEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]