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The map label for this gene is gcpE

Identifier: 15834947

GI number: 15834947

Start: 386603

End: 388408

Strand: Reverse

Name: gcpE

Synonym: TC0327

Alternate gene names: 15834947

Gene position: 388408-386603 (Counterclockwise)

Preceding gene: 15834948

Following gene: 15834946

Centisome position: 36.2

GC content: 40.86

Gene sequence:

>1806_bases
ATGGCAGAATCCTATCTACAAAATGGTTTTCGACGTAAAACTCTCTCAGTAAAAGTTGGGAATCTTTTTGTTGGTAGCGA
ACACTCTATCAAAATACAATCCATGACAACAACCGCCACAACGGATGTGGAGGGAACAGTTCGACAGATTTACGCTCTAC
AGGAATGTGGATGCGAAATCGTCCGTGTTACAGTTCAAGGATTGAAAGAAGTTGGTGCGTGTGAACAGATAAAAGACCGT
CTGGTTCAACAAAACGTAACAATCCCTCTTGTAGCAGATATTCATTTCTTTCCCCAAGCAGCTATACATGTCGCAGATTT
CGTCGATAAAGTACGCATTAACCCTGGGAATTATGTAGATAAACGTAATATGTTTTCTGGGAAGATCTACTCTGATGAGC
AATACACTCGTAGCTTAGAACGCTTATTCGAAAAGTTCTCTCCGCTCGTTGCAAAATGTAAACGCTTAGGAAGAGCTATG
CGCATAGGAGTCAATCATGGCTCCTTATCCGAGCGCATCATGCAACGTTATGGAGATACGATTGAGGGTATGGTCTTCTC
CGCTTTGGAATACGCTGAAGTCTGCGTCAATATGGATTATCATAATATTGTCTTCTCAATGAAATCCAGCAACCCTAGGA
CCATGGTGGCAGCTTATCGAGCTTTGGCTCGCGAGTTAGACCAAAGAAAGTGGTTATATCCTTTACACCTAGGAGTTACA
GAAGCTGGATCTGGCATGGATGGAATGATAAAATCCTCTGTAGGAATAGGAACCTTACTCTCCGAAGGACTTGGAGACAC
GATTCGTTGTTCTCTAACCGGCTCCCCAACCCTTGAAATTCCGGTTTGCTTAGATCTTCTTAAGGAAACAGCGAAATACT
CCAAATCTACAAAGAAATACAATCCTTTTGAAATCTACCATTCAAAACAACTTACTACTCAAACAACTCCAAAACATTTC
CCCGTAGAGAATGTTTATGGGTTTTTGGTCGGATTAACCAAGGATCATTTACTAACTATAGAACCTAATACGCTTCTCCA
GTGCTTGGGAGTCGATATCACAACTGGTAAAAAAGATCTTACATCACCTGACGGCGTGGTCATTCCAAAATCCATGCGTT
CTTCTGCTATCGTTTCCGAAATAGAGAAACACCTTCGTATCTTTTATAAGGAGGATGCTCCTATTCTTAATGAAATGAAT
GAGGAACTTTGGCTATCCGACAAAACCCTTGCCACTCCATTTGTGTATTTTGAAGTAGAAAGTATCTATCAAGCCCGTCG
CTTTTTTGCTTTGCGACAAAACTACTCTCAACCTGTTTGTTTGAGCTTTTCTCTAAAGGCTAATCTTTCTAAGAACTCTG
CAGCAATAGATTTGTCGATTCGTTTAGGAGCCCTACTTCTAGATGGTTTAGGATCCTGCGTACTATTCAATTTTTCTGAT
TTAAAACTCGCTAGAACTTTAGGATTTTCGATTTTACAAAGCGCAAACATTCGATCGACAACGGTAGAATACATCTCCTG
TCCTGGATGTGGTCGGACACTCTTTGACCTTCCTGAGGTTTCCCAACGTATTAAAGAAAGAACCAAGCATCTTCCTGGAG
GATTAAAAATTGCTGTCATGGGTTGTATCGTTAATGGACCGGGAGAAATGGCTGACGCAGATTTTGGCTATGTAGGCTCT
AAACCTGGAATGATCGATCTATACGTCAAACATAAATGCGTCAAATCCTATGTTCCTATGGAAAATGCTGAAGAAGAATT
AATCCAATTGCTAAAAGAACATGGTGTATGGAAAGAACCTGAATAG

Upstream 100 bases:

>100_bases
ATAAATCAAAAAAAGCTTACAATTATCCCTAAACTTTCTTTCTTGGGAGAGTGGAGTATGCTCTCAGAAATTCAATAAGT
TATTTGTTAGAAGCTTTATC

Downstream 100 bases:

>100_bases
GATAACCAATGACCTCTCCTTCTGAAGCTTTACATAAGCAAACTTTCCCCGAGCTGGAACACTTCCCTATCCGTCATGGA
ATTTTCCCAAAACAAAATGA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MAESYLQNGFRRKTLSVKVGNLFVGSEHSIKIQSMTTTATTDVEGTVRQIYALQECGCEIVRVTVQGLKEVGACEQIKDR
LVQQNVTIPLVADIHFFPQAAIHVADFVDKVRINPGNYVDKRNMFSGKIYSDEQYTRSLERLFEKFSPLVAKCKRLGRAM
RIGVNHGSLSERIMQRYGDTIEGMVFSALEYAEVCVNMDYHNIVFSMKSSNPRTMVAAYRALARELDQRKWLYPLHLGVT
EAGSGMDGMIKSSVGIGTLLSEGLGDTIRCSLTGSPTLEIPVCLDLLKETAKYSKSTKKYNPFEIYHSKQLTTQTTPKHF
PVENVYGFLVGLTKDHLLTIEPNTLLQCLGVDITTGKKDLTSPDGVVIPKSMRSSAIVSEIEKHLRIFYKEDAPILNEMN
EELWLSDKTLATPFVYFEVESIYQARRFFALRQNYSQPVCLSFSLKANLSKNSAAIDLSIRLGALLLDGLGSCVLFNFSD
LKLARTLGFSILQSANIRSTTVEYISCPGCGRTLFDLPEVSQRIKERTKHLPGGLKIAVMGCIVNGPGEMADADFGYVGS
KPGMIDLYVKHKCVKSYVPMENAEEELIQLLKEHGVWKEPE

Sequences:

>Translated_601_residues
MAESYLQNGFRRKTLSVKVGNLFVGSEHSIKIQSMTTTATTDVEGTVRQIYALQECGCEIVRVTVQGLKEVGACEQIKDR
LVQQNVTIPLVADIHFFPQAAIHVADFVDKVRINPGNYVDKRNMFSGKIYSDEQYTRSLERLFEKFSPLVAKCKRLGRAM
RIGVNHGSLSERIMQRYGDTIEGMVFSALEYAEVCVNMDYHNIVFSMKSSNPRTMVAAYRALARELDQRKWLYPLHLGVT
EAGSGMDGMIKSSVGIGTLLSEGLGDTIRCSLTGSPTLEIPVCLDLLKETAKYSKSTKKYNPFEIYHSKQLTTQTTPKHF
PVENVYGFLVGLTKDHLLTIEPNTLLQCLGVDITTGKKDLTSPDGVVIPKSMRSSAIVSEIEKHLRIFYKEDAPILNEMN
EELWLSDKTLATPFVYFEVESIYQARRFFALRQNYSQPVCLSFSLKANLSKNSAAIDLSIRLGALLLDGLGSCVLFNFSD
LKLARTLGFSILQSANIRSTTVEYISCPGCGRTLFDLPEVSQRIKERTKHLPGGLKIAVMGCIVNGPGEMADADFGYVGS
KPGMIDLYVKHKCVKSYVPMENAEEELIQLLKEHGVWKEPE
>Mature_600_residues
AESYLQNGFRRKTLSVKVGNLFVGSEHSIKIQSMTTTATTDVEGTVRQIYALQECGCEIVRVTVQGLKEVGACEQIKDRL
VQQNVTIPLVADIHFFPQAAIHVADFVDKVRINPGNYVDKRNMFSGKIYSDEQYTRSLERLFEKFSPLVAKCKRLGRAMR
IGVNHGSLSERIMQRYGDTIEGMVFSALEYAEVCVNMDYHNIVFSMKSSNPRTMVAAYRALARELDQRKWLYPLHLGVTE
AGSGMDGMIKSSVGIGTLLSEGLGDTIRCSLTGSPTLEIPVCLDLLKETAKYSKSTKKYNPFEIYHSKQLTTQTTPKHFP
VENVYGFLVGLTKDHLLTIEPNTLLQCLGVDITTGKKDLTSPDGVVIPKSMRSSAIVSEIEKHLRIFYKEDAPILNEMNE
ELWLSDKTLATPFVYFEVESIYQARRFFALRQNYSQPVCLSFSLKANLSKNSAAIDLSIRLGALLLDGLGSCVLFNFSDL
KLARTLGFSILQSANIRSTTVEYISCPGCGRTLFDLPEVSQRIKERTKHLPGGLKIAVMGCIVNGPGEMADADFGYVGSK
PGMIDLYVKHKCVKSYVPMENAEEELIQLLKEHGVWKEPE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=281, Percent_Identity=40.9252669039146, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CHLMU (Q9PKY3)

Other databases:

- EMBL:   AE002160
- PIR:   C81715
- RefSeq:   NP_296706.1
- ProteinModelPortal:   Q9PKY3
- GeneID:   1246370
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0327
- TIGR:   TC_0327
- HOGENOM:   HBG335271
- OMA:   SMRIGTN
- PhylomeDB:   Q9PKY3
- ProtClustDB:   PRK00694
- BioCyc:   CMUR243161:TC_0327-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR017178
- InterPro:   IPR004588
- PIRSF:   PIRSF037336
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 67107; Mature: 66976

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESYLQNGFRRKTLSVKVGNLFVGSEHSIKIQSMTTTATTDVEGTVRQIYALQECGCEI
CCCHHHHCCCCEEEEEEEECEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCHHH
VRVTVQGLKEVGACEQIKDRLVQQNVTIPLVADIHFFPQAAIHVADFVDKVRINPGNYVD
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHEECCCCCCCC
KRNMFSGKIYSDEQYTRSLERLFEKFSPLVAKCKRLGRAMRIGVNHGSLSERIMQRYGDT
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHCCH
IEGMVFSALEYAEVCVNMDYHNIVFSMKSSNPRTMVAAYRALARELDQRKWLYPLHLGVT
HHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCC
EAGSGMDGMIKSSVGIGTLLSEGLGDTIRCSLTGSPTLEIPVCLDLLKETAKYSKSTKKY
CCCCCCCCHHHHCCCHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCC
NPFEIYHSKQLTTQTTPKHFPVENVYGFLVGLTKDHLLTIEPNTLLQCLGVDITTGKKDL
CCEEEEECCCCCCCCCCCCCCHHHHHHHHEEECCCCEEEECCHHHHHHHCCEEECCCCCC
TSPDGVVIPKSMRSSAIVSEIEKHLRIFYKEDAPILNEMNEELWLSDKTLATPFVYFEVE
CCCCCEEECHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCHHHCCCCCCCCCCEEEEEHH
SIYQARRFFALRQNYSQPVCLSFSLKANLSKNSAAIDLSIRLGALLLDGLGSCVLFNFSD
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEEEHHHHHHHHCCCCEEEEECHH
LKLARTLGFSILQSANIRSTTVEYISCPGCGRTLFDLPEVSQRIKERTKHLPGGLKIAVM
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEEE
GCIVNGPGEMADADFGYVGSKPGMIDLYVKHKCVKSYVPMENAEEELIQLLKEHGVWKEP
EEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
E
C
>Mature Secondary Structure 
AESYLQNGFRRKTLSVKVGNLFVGSEHSIKIQSMTTTATTDVEGTVRQIYALQECGCEI
CCHHHHCCCCEEEEEEEECEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCHHH
VRVTVQGLKEVGACEQIKDRLVQQNVTIPLVADIHFFPQAAIHVADFVDKVRINPGNYVD
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHEECCCCCCCC
KRNMFSGKIYSDEQYTRSLERLFEKFSPLVAKCKRLGRAMRIGVNHGSLSERIMQRYGDT
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHCCH
IEGMVFSALEYAEVCVNMDYHNIVFSMKSSNPRTMVAAYRALARELDQRKWLYPLHLGVT
HHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCC
EAGSGMDGMIKSSVGIGTLLSEGLGDTIRCSLTGSPTLEIPVCLDLLKETAKYSKSTKKY
CCCCCCCCHHHHCCCHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCC
NPFEIYHSKQLTTQTTPKHFPVENVYGFLVGLTKDHLLTIEPNTLLQCLGVDITTGKKDL
CCEEEEECCCCCCCCCCCCCCHHHHHHHHEEECCCCEEEECCHHHHHHHCCEEECCCCCC
TSPDGVVIPKSMRSSAIVSEIEKHLRIFYKEDAPILNEMNEELWLSDKTLATPFVYFEVE
CCCCCEEECHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCHHHCCCCCCCCCCEEEEEHH
SIYQARRFFALRQNYSQPVCLSFSLKANLSKNSAAIDLSIRLGALLLDGLGSCVLFNFSD
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEEEHHHHHHHHCCCCEEEEECHH
LKLARTLGFSILQSANIRSTTVEYISCPGCGRTLFDLPEVSQRIKERTKHLPGGLKIAVM
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEEE
GCIVNGPGEMADADFGYVGSKPGMIDLYVKHKCVKSYVPMENAEEELIQLLKEHGVWKEP
EEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935