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Identifier: 15834932

GI number: 15834932

Start: 367870

End: 369870

Strand: Reverse

Name: Not Available

Synonym: TC0312

Alternate gene names: 15834932

Gene position: 369870-367870 (Counterclockwise)

Preceding gene: 15834933

Following gene: 15834929

Centisome position: 34.47

GC content: 39.28

Gene sequence:

>2001_bases
ATGGGATCTTTGTCTATTCACCTCACCACCCCGCTTCCCTTAGGAGCTCAAAAACTCTCTTCGGATCGTTATCGGTTCTC
GCTGGTTGCTCCCCGTGCGCAACAAGTCATTTTAGTACTACTAGATCCTTTTTCTGAGATTCATGAAATCCCTCTTTCTT
CTACAGATCATAGAACAGGAGCTATCTGGCATATAGAAATTTCAGGAATTTCAAACGAATGGTCTTACGCTTATAAACTT
AGACAATCGGATTCCGCTTTTCCTAATTTCTCTACAAATGCTTATATCGCTGATCCGTACTCTAAAAACATTTTCTCTCC
TCAGCTATTCGGATCATCTAAACAACCAAACGACTACACTTTTAGTTACTTAAAACAAGAAGATTTTGACTGGGAAGGGG
ATACTCCCTTAAGGCTTCCTAAAGAAAACTATTTCATCTATGAAATGCATGTTCGTTCGTTCACGCAGGACCCTTCTTCC
CAAGTTACGCATCCAGGAACCTTCCTTGGCATCATAGAAAAAATTGATCATCTTAAAAAACTGGGTGTTAATGCTGTCGA
ATTGCTTCCTATTTTTGAGTTTGATGAAACTATTCACCCATTTAAAAATAAAGATTTCCCCCACTTATGTAATTACTGGG
GGTACTCTTCGATTAATTTTTTCTGTCCTTCTCGCCGTTATACCTATGGAACAGATCCTTGCGCTCCTGCACGCGAATTT
AAAACTTTAGTAAAAACATTACACCGCGCAGGGATAGAAGTTATATTAGACGTAGTTTTCAATCACACGGGATTTGAAGG
AACAAGCTGCCCTCTCCCTTGGATAGATCTCGAGTCGTATTATATGGTCAATGACAATGGAGATCTTCTTAATTTTTCCG
GATGTGGAAATACAGTAAATACAAATACACCTATAGCCATTAAATGGATTCTTGATGCTTTGCGTTACTGGGTTCAAGAA
ATGCATGTAGATGGATTTCGTTTTGACTTAGCTGCAGTATTTTCAAGAGATTTGCAAGGAGTTCCCCGCTCTTTGACTCC
TATTCTGCAAGCAATATCGTCTGATTCCATTCTATCGGAAACCAAACTTATTGCAGAGCCTTGGGATGCAGGGGGACTCT
ATCAACTCGGGCACTTCCCTTCTATATCAACTAGGTGGAGCGAATGGAATGGGTGCTATCGCGACCACGTAAAAGCCTTC
CTTAATGGGGATCCTCATCAGGTTAGTTCTTTCGCGTCTCGAATATCCGGATCTCGTGATATCTATCCTGCAGGAAATTC
TACGAATTCTATTAATTATATTTGTTCTCATGACGGGTTCACTCTCTATGATAGCGTTGCCTATAATCACAAACATAATG
AAGAGAACGGAGAGAACAACCGTGACGGAACTTCGGCAAATTACAGCTATAATTTTGGATGTGAGGGAGAAACTACTGAC
CCAAACATTTGCCAGTTACGTGAACGACAAATGAAAAATTTCTTTCTCGCCCTCTTTTTATCCCAGGGAATCCCTATGAT
AAAATCTGGCGATGAATATGGTCATACTGCTTATGGAAACAACAACCATTGGTGCTTGGATACTAAAATTAATCACTTCC
TTTGGGATCGATTAGCTGAAAGAAAAGAATTTTTTTCTTTCCTGTGTCAAATCATTACTTTGCGTACAACGCATGCTGAA
CTATTCAACACAAATTTCTTATCAGAGGAGACAATAACCTGGCTAAATTCTCAAGGGTTGCCTAGAGAATGGACTCCAGA
CCACTATCTAGCATTTGAATTAAAACATCCCAATTACAGTCTATTTATAGCTTTCTACAGTGGAAATGAGCGTATTGAGA
TCGCTTTACCTAAACTAAGACAAGAACATCTAGCCTATGAGAAAATTGTGGATAGTACAACTGGCTTCTTTTCTCAAATC
TTATCTCCTAAACTCTCATTAGAACCATATAGCTCTTTGGTGGCAATTAGTAGAAAAAAGAAACCTATTGAACCCAAATA
A

Upstream 100 bases:

>100_bases
CCTCCAACAGGAATTCGTGCGTAGGTTTAGCTTTCATATGATAAAAGGAAGAGTCTCCCCTCTTCCTTTTTTTTATTTAA
CTTATGCATTCTAGTTTTTT

Downstream 100 bases:

>100_bases
AAAGACCGAACCCTATAAAGAGTTCGGTCCTTACACACAAAAGAACTTAATAAGTTCTTCAAACACCTCTATTATTGTCT
TCCCAAGACTCAATGACGAC

Product: glycosyl hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MGSLSIHLTTPLPLGAQKLSSDRYRFSLVAPRAQQVILVLLDPFSEIHEIPLSSTDHRTGAIWHIEISGISNEWSYAYKL
RQSDSAFPNFSTNAYIADPYSKNIFSPQLFGSSKQPNDYTFSYLKQEDFDWEGDTPLRLPKENYFIYEMHVRSFTQDPSS
QVTHPGTFLGIIEKIDHLKKLGVNAVELLPIFEFDETIHPFKNKDFPHLCNYWGYSSINFFCPSRRYTYGTDPCAPAREF
KTLVKTLHRAGIEVILDVVFNHTGFEGTSCPLPWIDLESYYMVNDNGDLLNFSGCGNTVNTNTPIAIKWILDALRYWVQE
MHVDGFRFDLAAVFSRDLQGVPRSLTPILQAISSDSILSETKLIAEPWDAGGLYQLGHFPSISTRWSEWNGCYRDHVKAF
LNGDPHQVSSFASRISGSRDIYPAGNSTNSINYICSHDGFTLYDSVAYNHKHNEENGENNRDGTSANYSYNFGCEGETTD
PNICQLRERQMKNFFLALFLSQGIPMIKSGDEYGHTAYGNNNHWCLDTKINHFLWDRLAERKEFFSFLCQIITLRTTHAE
LFNTNFLSEETITWLNSQGLPREWTPDHYLAFELKHPNYSLFIAFYSGNERIEIALPKLRQEHLAYEKIVDSTTGFFSQI
LSPKLSLEPYSSLVAISRKKKPIEPK

Sequences:

>Translated_666_residues
MGSLSIHLTTPLPLGAQKLSSDRYRFSLVAPRAQQVILVLLDPFSEIHEIPLSSTDHRTGAIWHIEISGISNEWSYAYKL
RQSDSAFPNFSTNAYIADPYSKNIFSPQLFGSSKQPNDYTFSYLKQEDFDWEGDTPLRLPKENYFIYEMHVRSFTQDPSS
QVTHPGTFLGIIEKIDHLKKLGVNAVELLPIFEFDETIHPFKNKDFPHLCNYWGYSSINFFCPSRRYTYGTDPCAPAREF
KTLVKTLHRAGIEVILDVVFNHTGFEGTSCPLPWIDLESYYMVNDNGDLLNFSGCGNTVNTNTPIAIKWILDALRYWVQE
MHVDGFRFDLAAVFSRDLQGVPRSLTPILQAISSDSILSETKLIAEPWDAGGLYQLGHFPSISTRWSEWNGCYRDHVKAF
LNGDPHQVSSFASRISGSRDIYPAGNSTNSINYICSHDGFTLYDSVAYNHKHNEENGENNRDGTSANYSYNFGCEGETTD
PNICQLRERQMKNFFLALFLSQGIPMIKSGDEYGHTAYGNNNHWCLDTKINHFLWDRLAERKEFFSFLCQIITLRTTHAE
LFNTNFLSEETITWLNSQGLPREWTPDHYLAFELKHPNYSLFIAFYSGNERIEIALPKLRQEHLAYEKIVDSTTGFFSQI
LSPKLSLEPYSSLVAISRKKKPIEPK
>Mature_665_residues
GSLSIHLTTPLPLGAQKLSSDRYRFSLVAPRAQQVILVLLDPFSEIHEIPLSSTDHRTGAIWHIEISGISNEWSYAYKLR
QSDSAFPNFSTNAYIADPYSKNIFSPQLFGSSKQPNDYTFSYLKQEDFDWEGDTPLRLPKENYFIYEMHVRSFTQDPSSQ
VTHPGTFLGIIEKIDHLKKLGVNAVELLPIFEFDETIHPFKNKDFPHLCNYWGYSSINFFCPSRRYTYGTDPCAPAREFK
TLVKTLHRAGIEVILDVVFNHTGFEGTSCPLPWIDLESYYMVNDNGDLLNFSGCGNTVNTNTPIAIKWILDALRYWVQEM
HVDGFRFDLAAVFSRDLQGVPRSLTPILQAISSDSILSETKLIAEPWDAGGLYQLGHFPSISTRWSEWNGCYRDHVKAFL
NGDPHQVSSFASRISGSRDIYPAGNSTNSINYICSHDGFTLYDSVAYNHKHNEENGENNRDGTSANYSYNFGCEGETTDP
NICQLRERQMKNFFLALFLSQGIPMIKSGDEYGHTAYGNNNHWCLDTKINHFLWDRLAERKEFFSFLCQIITLRTTHAEL
FNTNFLSEETITWLNSQGLPREWTPDHYLAFELKHPNYSLFIAFYSGNERIEIALPKLRQEHLAYEKIVDSTTGFFSQIL
SPKLSLEPYSSLVAISRKKKPIEPK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=602, Percent_Identity=35.0498338870432, Blast_Score=326, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 76233; Mature: 76102

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLSIHLTTPLPLGAQKLSSDRYRFSLVAPRAQQVILVLLDPFSEIHEIPLSSTDHRTG
CCCEEEEEECCCCCCHHHHCCCCEEEEEECCCCCEEEEEEECCHHHHHHCCCCCCCCCCC
AIWHIEISGISNEWSYAYKLRQSDSAFPNFSTNAYIADPYSKNIFSPQLFGSSKQPNDYT
EEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCC
FSYLKQEDFDWEGDTPLRLPKENYFIYEMHVRSFTQDPSSQVTHPGTFLGIIEKIDHLKK
HHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LGVNAVELLPIFEFDETIHPFKNKDFPHLCNYWGYSSINFFCPSRRYTYGTDPCAPAREF
HCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHH
KTLVKTLHRAGIEVILDVVFNHTGFEGTSCPLPWIDLESYYMVNDNGDLLNFSGCGNTVN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEECCCCCEEEECCCCCCCC
TNTPIAIKWILDALRYWVQEMHVDGFRFDLAAVFSRDLQGVPRSLTPILQAISSDSILSE
CCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
TKLIAEPWDAGGLYQLGHFPSISTRWSEWNGCYRDHVKAFLNGDPHQVSSFASRISGSRD
HHHHCCCCCCCCEEECCCCCCCCCCCHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
IYPAGNSTNSINYICSHDGFTLYDSVAYNHKHNEENGENNRDGTSANYSYNFGCEGETTD
EECCCCCCCCEEEEECCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
PNICQLRERQMKNFFLALFLSQGIPMIKSGDEYGHTAYGNNNHWCLDTKINHFLWDRLAE
CHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
RKEFFSFLCQIITLRTTHAELFNTNFLSEETITWLNSQGLPREWTPDHYLAFELKHPNYS
HHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEE
LFIAFYSGNERIEIALPKLRQEHLAYEKIVDSTTGFFSQILSPKLSLEPYSSLVAISRKK
EEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
KPIEPK
CCCCCC
>Mature Secondary Structure 
GSLSIHLTTPLPLGAQKLSSDRYRFSLVAPRAQQVILVLLDPFSEIHEIPLSSTDHRTG
CCEEEEEECCCCCCHHHHCCCCEEEEEECCCCCEEEEEEECCHHHHHHCCCCCCCCCCC
AIWHIEISGISNEWSYAYKLRQSDSAFPNFSTNAYIADPYSKNIFSPQLFGSSKQPNDYT
EEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCC
FSYLKQEDFDWEGDTPLRLPKENYFIYEMHVRSFTQDPSSQVTHPGTFLGIIEKIDHLKK
HHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LGVNAVELLPIFEFDETIHPFKNKDFPHLCNYWGYSSINFFCPSRRYTYGTDPCAPAREF
HCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHH
KTLVKTLHRAGIEVILDVVFNHTGFEGTSCPLPWIDLESYYMVNDNGDLLNFSGCGNTVN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEECCCCCEEEECCCCCCCC
TNTPIAIKWILDALRYWVQEMHVDGFRFDLAAVFSRDLQGVPRSLTPILQAISSDSILSE
CCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
TKLIAEPWDAGGLYQLGHFPSISTRWSEWNGCYRDHVKAFLNGDPHQVSSFASRISGSRD
HHHHCCCCCCCCEEECCCCCCCCCCCHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
IYPAGNSTNSINYICSHDGFTLYDSVAYNHKHNEENGENNRDGTSANYSYNFGCEGETTD
EECCCCCCCCEEEEECCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
PNICQLRERQMKNFFLALFLSQGIPMIKSGDEYGHTAYGNNNHWCLDTKINHFLWDRLAE
CHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
RKEFFSFLCQIITLRTTHAELFNTNFLSEETITWLNSQGLPREWTPDHYLAFELKHPNYS
HHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEE
LFIAFYSGNERIEIALPKLRQEHLAYEKIVDSTTGFFSQILSPKLSLEPYSSLVAISRKK
EEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
KPIEPK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]