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The map label for this gene is metG

Identifier: 15834921

GI number: 15834921

Start: 356858

End: 358510

Strand: Direct

Name: metG

Synonym: TC0301

Alternate gene names: 15834921

Gene position: 356858-358510 (Clockwise)

Preceding gene: 15834920

Following gene: 15834924

Centisome position: 33.26

GC content: 39.65

Gene sequence:

>1653_bases
GTGGCATCTTCTCGCATTCTTATAACTTCTGCTTTGCCTTATGCAAATGGACCTCTTCATTTCGGACACATTACGGGTGC
TTATTTACCTGCAGATGTTTATGCGCGTTTTCAAAGATTGCTAGGAAAAGAAGTATTATACATTTGTGGTTCTGATGAGT
ACGGAATAGCGATTACCCTAAATGCAGAATTAGCAGGTATGGGGTATCAAGAATATGTTGATATGTATCATAAGCTTCAT
AAAGATACCCTTAAGAAGCTCGGGATTTCTGTAGATTTCTTTTCCAGAACCACAAACCCTCATCATCCCGCTATTGTTCA
GGATTTCTATAGAAATTTGCAGGACAAAGGGTTGATGGAAAACCTTGTAACGGATCAGCTTTATTCTGAGGAAGAAGAAA
AGTTTTTAGCTGACCGTTATGTTGTGGGGACCTGTCCTAAGTGTGGGTTTGATCGGGCTAGGGGAGACGAATGTCAGCAG
TGTGGAGCCGATTACGAAGCCAGAGATTTAAAAGATCCTCGTTCTAAATTAACTGGGAAAGCTTTGTCTTTGCGCGAAAC
AGAACATGCTTATTTTCATTTAGAACGCATGAAGGAAGAACTGCTTACCTTTGTAGAGGGCATTTATTTACGTCCCCACA
TGCGTAACTTTGTAATAGATTACATTAAAAATTTGCGTCCTCGAGCAGTTACTAGAGATTTGTCTTGGGGGGTACCCGTT
CCAGGTTTAGAGAACAAAGTATTCTATGTATGGTTTGATGCTCCAATCGGATACATTAGTGGAACTATGGATTGGGCAGC
GTCTATCGGAGATCCAGACGCTTGGAAGAAATTTTGGTTAGATGACAAAGTAACGTATACTCAGTTCGTTGGTAAAGATA
ATACATCTTTCCATTCGGTTATTTTTCCTGCTATGGAAATGGGACAGTCGCTTCCCTATAAGAAAGTCGATGCTTTAGTG
ACATCCGAATTTTTACTTTTGGAAGGATTCCAATTTAGTAAATCGGAAGGGAATATCATAGATATGGATGCGTTTTTAGA
AACGTATTCCTTGGATAAATTACGCTATGTGTTAGCGGCTATTGCTCCAGAAACTTCGGATAGCGAGTTTGCATTCCAGG
AGTTTAAAACTCGATGCAATTCTGATCTTGTAGGTAAATTCGGAAATTTTGTGAATCGAGTTGTTGCTTTTGCTGCTAAG
AATGGATGCACCGAACTTTCTCATCCTCAATTAGAACAACAAGATTTAGAGTTCATAGCTGAAGCTCAAAAACTTGCTAA
AGAAGCTGCTGATCATTATCAGCAATATAGTTTGCGTAAAGCTTGCTCAACAATTATGGAATTAGCTGCCTTGGGGAATG
GTTATTTCAACGATCAAGCTCCATGGAAACTAGCTAAAGAAGATCTTTGGAATCGAGTGCGATCCATTCTATTTTGTGCT
TGCTATTGCCAAAAATTGTTAGCTCTTATCTCCTATCCAATTATGCCTGAAACAGCGTGGACAATTTGGGGAATGATTGC
TCCAGGTTCTTTAGATTTGCGTTCTCAAGATCCGGARAGATTGCAGTCAATTTGGACAGAAGCCTTTTTCGATTACTCAG
AAGAGAGCTTCTCGTTGAAAGAACCTGAATTATTGTTCACTGTAGTTGAGTGA

Upstream 100 bases:

>100_bases
AGCAATATGCGTCCGGAGCAGGAGAAAAGCGAAAAGAACAAAGTTCTGGAAACTCCAGAAGAAAGGATCCGTCTCTGTAC
AATTGGAGCGACGTGAAATA

Downstream 100 bases:

>100_bases
TTTTTTTTATTGAATTTCATTTTGAAAGAGGGATTCTATGTACTAGAATCCTTTTTTTTTTGACTTGGGATTTATCTATT
AGAGAAAATTACAGCTTGTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MASSRILITSALPYANGPLHFGHITGAYLPADVYARFQRLLGKEVLYICGSDEYGIAITLNAELAGMGYQEYVDMYHKLH
KDTLKKLGISVDFFSRTTNPHHPAIVQDFYRNLQDKGLMENLVTDQLYSEEEEKFLADRYVVGTCPKCGFDRARGDECQQ
CGADYEARDLKDPRSKLTGKALSLRETEHAYFHLERMKEELLTFVEGIYLRPHMRNFVIDYIKNLRPRAVTRDLSWGVPV
PGLENKVFYVWFDAPIGYISGTMDWAASIGDPDAWKKFWLDDKVTYTQFVGKDNTSFHSVIFPAMEMGQSLPYKKVDALV
TSEFLLLEGFQFSKSEGNIIDMDAFLETYSLDKLRYVLAAIAPETSDSEFAFQEFKTRCNSDLVGKFGNFVNRVVAFAAK
NGCTELSHPQLEQQDLEFIAEAQKLAKEAADHYQQYSLRKACSTIMELAALGNGYFNDQAPWKLAKEDLWNRVRSILFCA
CYCQKLLALISYPIMPETAWTIWGMIAPGSLDLRSQDPERLQSIWTEAFFDYSEESFSLKEPELLFTVVE

Sequences:

>Translated_550_residues
MASSRILITSALPYANGPLHFGHITGAYLPADVYARFQRLLGKEVLYICGSDEYGIAITLNAELAGMGYQEYVDMYHKLH
KDTLKKLGISVDFFSRTTNPHHPAIVQDFYRNLQDKGLMENLVTDQLYSEEEEKFLADRYVVGTCPKCGFDRARGDECQQ
CGADYEARDLKDPRSKLTGKALSLRETEHAYFHLERMKEELLTFVEGIYLRPHMRNFVIDYIKNLRPRAVTRDLSWGVPV
PGLENKVFYVWFDAPIGYISGTMDWAASIGDPDAWKKFWLDDKVTYTQFVGKDNTSFHSVIFPAMEMGQSLPYKKVDALV
TSEFLLLEGFQFSKSEGNIIDMDAFLETYSLDKLRYVLAAIAPETSDSEFAFQEFKTRCNSDLVGKFGNFVNRVVAFAAK
NGCTELSHPQLEQQDLEFIAEAQKLAKEAADHYQQYSLRKACSTIMELAALGNGYFNDQAPWKLAKEDLWNRVRSILFCA
CYCQKLLALISYPIMPETAWTIWGMIAPGSLDLRSQDPERLQSIWTEAFFDYSEESFSLKEPELLFTVVE
>Mature_549_residues
ASSRILITSALPYANGPLHFGHITGAYLPADVYARFQRLLGKEVLYICGSDEYGIAITLNAELAGMGYQEYVDMYHKLHK
DTLKKLGISVDFFSRTTNPHHPAIVQDFYRNLQDKGLMENLVTDQLYSEEEEKFLADRYVVGTCPKCGFDRARGDECQQC
GADYEARDLKDPRSKLTGKALSLRETEHAYFHLERMKEELLTFVEGIYLRPHMRNFVIDYIKNLRPRAVTRDLSWGVPVP
GLENKVFYVWFDAPIGYISGTMDWAASIGDPDAWKKFWLDDKVTYTQFVGKDNTSFHSVIFPAMEMGQSLPYKKVDALVT
SEFLLLEGFQFSKSEGNIIDMDAFLETYSLDKLRYVLAAIAPETSDSEFAFQEFKTRCNSDLVGKFGNFVNRVVAFAAKN
GCTELSHPQLEQQDLEFIAEAQKLAKEAADHYQQYSLRKACSTIMELAALGNGYFNDQAPWKLAKEDLWNRVRSILFCAC
YCQKLLALISYPIMPETAWTIWGMIAPGSLDLRSQDPERLQSIWTEAFFDYSEESFSLKEPELLFTVVE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=508, Percent_Identity=36.0236220472441, Blast_Score=319, Evalue=5e-87,
Organism=Homo sapiens, GI24308436, Length=524, Percent_Identity=27.0992366412214, Blast_Score=169, Evalue=8e-42,
Organism=Escherichia coli, GI1788432, Length=505, Percent_Identity=31.4851485148515, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1786861, Length=147, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=510, Percent_Identity=37.0588235294118, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI212645096, Length=518, Percent_Identity=27.4131274131274, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71992781, Length=368, Percent_Identity=27.445652173913, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321704, Length=521, Percent_Identity=33.5892514395393, Blast_Score=300, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=22.9906542056075, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24655311, Length=527, Percent_Identity=35.1043643263757, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21358483, Length=513, Percent_Identity=24.9512670565302, Blast_Score=139, Evalue=7e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CHLMU (Q9PL07)

Other databases:

- EMBL:   AE002160
- PIR:   H81718
- RefSeq:   NP_296680.1
- ProteinModelPortal:   Q9PL07
- SMR:   Q9PL07
- GeneID:   1246471
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0301
- TIGR:   TC_0301
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- PhylomeDB:   Q9PL07
- ProtClustDB:   PRK12268
- BioCyc:   CMUR243161:TC_0301-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 62815; Mature: 62684

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 334-334

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSRILITSALPYANGPLHFGHITGAYLPADVYARFQRLLGKEVLYICGSDEYGIAITL
CCCCCEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
NAELAGMGYQEYVDMYHKLHKDTLKKLGISVDFFSRTTNPHHPAIVQDFYRNLQDKGLME
CCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHCCCCCCCCHHHHHHHHHHHHCHHHH
NLVTDQLYSEEEEKFLADRYVVGTCPKCGFDRARGDECQQCGADYEARDLKDPRSKLTGK
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHCCCCCCHHCCCCHHHHHCCC
ALSLRETEHAYFHLERMKEELLTFVEGIYLRPHMRNFVIDYIKNLRPRAVTRDLSWGVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
PGLENKVFYVWFDAPIGYISGTMDWAASIGDPDAWKKFWLDDKVTYTQFVGKDNTSFHSV
CCCCCCEEEEEECCCHHHHCCCHHHHHCCCCHHHHHHHHCCCCCCHHHHHCCCCCCHHHH
IFPAMEMGQSLPYKKVDALVTSEFLLLEGFQFSKSEGNIIDMDAFLETYSLDKLRYVLAA
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
IAPETSDSEFAFQEFKTRCNSDLVGKFGNFVNRVVAFAAKNGCTELSHPQLEQQDLEFIA
HCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
EAQKLAKEAADHYQQYSLRKACSTIMELAALGNGYFNDQAPWKLAKEDLWNRVRSILFCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
CYCQKLLALISYPIMPETAWTIWGMIAPGSLDLRSQDPERLQSIWTEAFFDYSEESFSLK
HHHHHHHHHHCCCCCCCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
EPELLFTVVE
CCCEEEEECC
>Mature Secondary Structure 
ASSRILITSALPYANGPLHFGHITGAYLPADVYARFQRLLGKEVLYICGSDEYGIAITL
CCCCEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
NAELAGMGYQEYVDMYHKLHKDTLKKLGISVDFFSRTTNPHHPAIVQDFYRNLQDKGLME
CCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHCCCCCCCCHHHHHHHHHHHHCHHHH
NLVTDQLYSEEEEKFLADRYVVGTCPKCGFDRARGDECQQCGADYEARDLKDPRSKLTGK
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHCCCCCCHHCCCCHHHHHCCC
ALSLRETEHAYFHLERMKEELLTFVEGIYLRPHMRNFVIDYIKNLRPRAVTRDLSWGVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
PGLENKVFYVWFDAPIGYISGTMDWAASIGDPDAWKKFWLDDKVTYTQFVGKDNTSFHSV
CCCCCCEEEEEECCCHHHHCCCHHHHHCCCCHHHHHHHHCCCCCCHHHHHCCCCCCHHHH
IFPAMEMGQSLPYKKVDALVTSEFLLLEGFQFSKSEGNIIDMDAFLETYSLDKLRYVLAA
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
IAPETSDSEFAFQEFKTRCNSDLVGKFGNFVNRVVAFAAKNGCTELSHPQLEQQDLEFIA
HCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
EAQKLAKEAADHYQQYSLRKACSTIMELAALGNGYFNDQAPWKLAKEDLWNRVRSILFCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
CYCQKLLALISYPIMPETAWTIWGMIAPGSLDLRSQDPERLQSIWTEAFFDYSEESFSLK
HHHHHHHHHHCCCCCCCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
EPELLFTVVE
CCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935