Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 15834916

GI number: 15834916

Start: 353791

End: 354849

Strand: Direct

Name: trmD

Synonym: TC0296

Alternate gene names: 15834916

Gene position: 353791-354849 (Clockwise)

Preceding gene: 15834915

Following gene: 15834917

Centisome position: 32.97

GC content: 43.15

Gene sequence:

>1059_bases
ATGGAGATAGATATTCTTTCTTTATTCCCGGACTATTTTACTAGTCCACTACAAGCAACCATTTTGGGCCGAGCTATCAA
GCAGGGAGCTTTATCTATTCGTTCCCGCGATATTCGAGAGTTTGGTTTAGGGAAATGGAAGCAAGTAGACGATGCCCCTT
ATAGTGGGGAGGGAATGCTTTTGATGGCTGAGCCGGTTGTGCAGGCTATTAGAAGCGTAAAAAGAGAGAAATCTAAAGTT
GTATACCTATCCCCGCAGGGGCAGCTTCTTTCTGCTAAAAAGAGTCGAGAGCTCTCGTTGTGTCCGCATTTAATTTTGTT
ATGTGGACATTATGAAGGGATTGATGAAAGAGCTTTAGCTTCTGAAGTGGATGAAGAGATAAGTATTGGTGATTACGTTC
TTACCAATGGGTGTGCGGCAGCGTTGGTTCTTGTAGACGCTCTTGCTCGCTTTATTCCAGGCGTTTTGGGAAACCAAGAA
AGTGCGGAATGCGATTCTCTTGAAAATGGGTTGTTAGAGGGGCCTCATTACACCCGTCCACGAGTTTTTGAAGGCGTACA
AGTTCCAGAGATACTTTTTTGTGGAGATCACCAAAGAATCGCAAATTGGCGAAAGAAGGTTAGTTTAGAGAGAACAAGAG
AACGCCGACCGGACCTGTATCTGCAGTATTTTTATGGTGATAGAGCTTTTTGGGGTGCCCAGGAGGATCCTCTTAAAATG
AGAGAAACTTCTCCCCAAGCTTTTTCTGTAGTTTTAGAAGTTAAAGATCTTCGAAAAGCTAAAAAATTTTATTCCAGAAT
GTTCGGAAAAGAACACTGGGATGGGGATAAATTACTCCTTGGGGAGAAGACGAGTCTGTATCTGCAACAGACAAATGAAA
GAAGGGGTGCAACCAAGGTATTTGTAGAGTTAGAAACTGAGGATGGTTTTGTCCGCTTTTTAAGAAGGTGGGAAATGCTT
GGTGGACAGCTCGGAGAGGTTGGAATGGGAAATCTACCTCTAAGGCAGGTTTTTGATTTGGATGGCCATATTTGGGTTGT
CTCTTGTGTACAGAAATAG

Upstream 100 bases:

>100_bases
AATGCTCGTAGAGCAGTCGTTAGACAAAAGAGACGAGCTTATCGTCAGCGACTTGCTGCGAGAAGGGCAGAAGCAGCTGC
TAAGTAAAAGGATATAAGGG

Downstream 100 bases:

>100_bases
AAAAAGAATTTTAGGTGAAATACAATGGGGAACTTAATTAAGGAATTGCAAGACGAGCAGTGCAGAACTGATCTCGTTGA
TTTCTGTGTTGGTGACACGA

Product: tRNA (guanine-N(1)-)-methyltransferase/unknown domain fusion protein

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MEIDILSLFPDYFTSPLQATILGRAIKQGALSIRSRDIREFGLGKWKQVDDAPYSGEGMLLMAEPVVQAIRSVKREKSKV
VYLSPQGQLLSAKKSRELSLCPHLILLCGHYEGIDERALASEVDEEISIGDYVLTNGCAAALVLVDALARFIPGVLGNQE
SAECDSLENGLLEGPHYTRPRVFEGVQVPEILFCGDHQRIANWRKKVSLERTRERRPDLYLQYFYGDRAFWGAQEDPLKM
RETSPQAFSVVLEVKDLRKAKKFYSRMFGKEHWDGDKLLLGEKTSLYLQQTNERRGATKVFVELETEDGFVRFLRRWEML
GGQLGEVGMGNLPLRQVFDLDGHIWVVSCVQK

Sequences:

>Translated_352_residues
MEIDILSLFPDYFTSPLQATILGRAIKQGALSIRSRDIREFGLGKWKQVDDAPYSGEGMLLMAEPVVQAIRSVKREKSKV
VYLSPQGQLLSAKKSRELSLCPHLILLCGHYEGIDERALASEVDEEISIGDYVLTNGCAAALVLVDALARFIPGVLGNQE
SAECDSLENGLLEGPHYTRPRVFEGVQVPEILFCGDHQRIANWRKKVSLERTRERRPDLYLQYFYGDRAFWGAQEDPLKM
RETSPQAFSVVLEVKDLRKAKKFYSRMFGKEHWDGDKLLLGEKTSLYLQQTNERRGATKVFVELETEDGFVRFLRRWEML
GGQLGEVGMGNLPLRQVFDLDGHIWVVSCVQK
>Mature_352_residues
MEIDILSLFPDYFTSPLQATILGRAIKQGALSIRSRDIREFGLGKWKQVDDAPYSGEGMLLMAEPVVQAIRSVKREKSKV
VYLSPQGQLLSAKKSRELSLCPHLILLCGHYEGIDERALASEVDEEISIGDYVLTNGCAAALVLVDALARFIPGVLGNQE
SAECDSLENGLLEGPHYTRPRVFEGVQVPEILFCGDHQRIANWRKKVSLERTRERRPDLYLQYFYGDRAFWGAQEDPLKM
RETSPQAFSVVLEVKDLRKAKKFYSRMFGKEHWDGDKLLLGEKTSLYLQQTNERRGATKVFVELETEDGFVRFLRRWEML
GGQLGEVGMGNLPLRQVFDLDGHIWVVSCVQK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=223, Percent_Identity=49.7757847533632, Blast_Score=204, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CHLMU (Q9PL12)

Other databases:

- EMBL:   AE002160
- PIR:   D81718
- RefSeq:   NP_296675.1
- ProteinModelPortal:   Q9PL12
- SMR:   Q9PL12
- GeneID:   1246466
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0296
- TIGR:   TC_0296
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- PhylomeDB:   Q9PL12
- ProtClustDB:   PRK01037
- BioCyc:   CMUR243161:TC_0296-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 39974; Mature: 39974

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIDILSLFPDYFTSPLQATILGRAIKQGALSIRSRDIREFGLGKWKQVDDAPYSGEGML
CCEEHEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LMAEPVVQAIRSVKREKSKVVYLSPQGQLLSAKKSRELSLCPHLILLCGHYEGIDERALA
EEHHHHHHHHHHHHHHCCCEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
SEVDEEISIGDYVLTNGCAAALVLVDALARFIPGVLGNQESAECDSLENGLLEGPHYTRP
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHHCCCCCCCCCCCC
RVFEGVQVPEILFCGDHQRIANWRKKVSLERTRERRPDLYLQYFYGDRAFWGAQEDPLKM
HHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCHH
RETSPQAFSVVLEVKDLRKAKKFYSRMFGKEHWDGDKLLLGEKTSLYLQQTNERRGATKV
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCCCCEEE
FVELETEDGFVRFLRRWEMLGGQLGEVGMGNLPLRQVFDLDGHIWVVSCVQK
EEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MEIDILSLFPDYFTSPLQATILGRAIKQGALSIRSRDIREFGLGKWKQVDDAPYSGEGML
CCEEHEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LMAEPVVQAIRSVKREKSKVVYLSPQGQLLSAKKSRELSLCPHLILLCGHYEGIDERALA
EEHHHHHHHHHHHHHHCCCEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
SEVDEEISIGDYVLTNGCAAALVLVDALARFIPGVLGNQESAECDSLENGLLEGPHYTRP
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHHCCCCCCCCCCCC
RVFEGVQVPEILFCGDHQRIANWRKKVSLERTRERRPDLYLQYFYGDRAFWGAQEDPLKM
HHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCHH
RETSPQAFSVVLEVKDLRKAKKFYSRMFGKEHWDGDKLLLGEKTSLYLQQTNERRGATKV
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCCCCEEE
FVELETEDGFVRFLRRWEMLGGQLGEVGMGNLPLRQVFDLDGHIWVVSCVQK
EEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935