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The map label for this gene is prfA

Identifier: 15834912

GI number: 15834912

Start: 350158

End: 351237

Strand: Direct

Name: prfA

Synonym: TC0292

Alternate gene names: 15834912

Gene position: 350158-351237 (Clockwise)

Preceding gene: 15834911

Following gene: 15834913

Centisome position: 32.64

GC content: 44.35

Gene sequence:

>1080_bases
ATGGAAGTAAAAGTTTTAGAGTGTTTGAGACGCCTTGAAGAAGTCGAAAAGCTAATATCTGATCCAAATATTTTTAGTAA
TCCCAAGGAATATAGTTCTTTAAGTAAGGAGCATGCTCGTCTTTCGGAGATTAAAAATGCTCACGAATCGATTCTTGCGG
CAAAAAAAATTCTTCATGATGACAAGTTAGCCTTGTCAACAGAGAAGGATCCGGAGATGATTGCTATGTTGGAAGAGGGT
ATTCAAGGAGGAGAGGAGTCCTTAGAACGTCTGTCTAAGCAATTGGAAAACCTTCTCATCCCACCTGATCCGGATGATGA
TTTAAGTGTCATTATGGAGTTGCGAGCAGGGACAGGAGGAGATGAAGCGGCTCTTTTCGTAGGCGATTGTGTGCGAATGT
ATCATCTTTATGCATCCATCAAAGGGTGGCAGTGTGAAGTGCTTTCTGCCTCGGAGTCCGATCTTGGTGGATACAAAGAA
TATATCATGGGAGTTTCAGGGACCTCTGTGAAACGATTCTTACAATATGAGGCAGGGACGCATCGTGTCCAAAGAGTTCC
GGAGACGGAAACTCAGGGAAGAGTGCATACTTCGGCCGTGACTATTGCTGTCCTTCCTGAACCTGCAGAAGATGACGAGG
AAGTTTTTATTGATGAGAAGGATCTACGTATAGATACCTTCCGTTCTTCAGGAGCTGGAGGGCAGCACGTAAACGTTACA
GATTCTGCCGTACGTATTACGCATATTCCTACTGGCGTGGTGGTTTCCTGTCAAGATGAGCGTAGTCAACATAAAAATAA
GGCGAAAGCTATGCGAGTGTTGAAGGCGCGTATTCGAGATGCGGAAGTGCAGAAGCGAGAACAGGAGGCTTCTGCTATGC
GTTCAGCGCAAGTAGGGAGTGGGGATCGCTCAGAAAGAATTAGGACTTATAATTTTCCTCAAAATCGTGTGACTGATCAT
CGAATCGGTCTGACTTTATATAACTTGGATCGTGTAATGCAGGGAGAGTTGGATATGATTACAACAGCTCTTGTTTCCCA
TGCACATCGCCAACTGTTCGGTCATGAAGAGACTAATTAA

Upstream 100 bases:

>100_bases
GAAGTTGTCTCTTCTGTCAAAGTCTAGTGTTCCCCAAAATTGATTGCCAATGTACACTCTGATCTTTGTTTGAAAGACAC
CAGAGTAGAAGAAGTCGTTC

Downstream 100 bases:

>100_bases
GGAAGCTTCAGAATATCTGTTATCTCGAGGGATTCGGTTTCCTCAAAGGGAAGCCGAAGATATCATGATGGATTTACTAG
AGGTTTCTTCTAGAGGGGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MEVKVLECLRRLEEVEKLISDPNIFSNPKEYSSLSKEHARLSEIKNAHESILAAKKILHDDKLALSTEKDPEMIAMLEEG
IQGGEESLERLSKQLENLLIPPDPDDDLSVIMELRAGTGGDEAALFVGDCVRMYHLYASIKGWQCEVLSASESDLGGYKE
YIMGVSGTSVKRFLQYEAGTHRVQRVPETETQGRVHTSAVTIAVLPEPAEDDEEVFIDEKDLRIDTFRSSGAGGQHVNVT
DSAVRITHIPTGVVVSCQDERSQHKNKAKAMRVLKARIRDAEVQKREQEASAMRSAQVGSGDRSERIRTYNFPQNRVTDH
RIGLTLYNLDRVMQGELDMITTALVSHAHRQLFGHEETN

Sequences:

>Translated_359_residues
MEVKVLECLRRLEEVEKLISDPNIFSNPKEYSSLSKEHARLSEIKNAHESILAAKKILHDDKLALSTEKDPEMIAMLEEG
IQGGEESLERLSKQLENLLIPPDPDDDLSVIMELRAGTGGDEAALFVGDCVRMYHLYASIKGWQCEVLSASESDLGGYKE
YIMGVSGTSVKRFLQYEAGTHRVQRVPETETQGRVHTSAVTIAVLPEPAEDDEEVFIDEKDLRIDTFRSSGAGGQHVNVT
DSAVRITHIPTGVVVSCQDERSQHKNKAKAMRVLKARIRDAEVQKREQEASAMRSAQVGSGDRSERIRTYNFPQNRVTDH
RIGLTLYNLDRVMQGELDMITTALVSHAHRQLFGHEETN
>Mature_359_residues
MEVKVLECLRRLEEVEKLISDPNIFSNPKEYSSLSKEHARLSEIKNAHESILAAKKILHDDKLALSTEKDPEMIAMLEEG
IQGGEESLERLSKQLENLLIPPDPDDDLSVIMELRAGTGGDEAALFVGDCVRMYHLYASIKGWQCEVLSASESDLGGYKE
YIMGVSGTSVKRFLQYEAGTHRVQRVPETETQGRVHTSAVTIAVLPEPAEDDEEVFIDEKDLRIDTFRSSGAGGQHVNVT
DSAVRITHIPTGVVVSCQDERSQHKNKAKAMRVLKARIRDAEVQKREQEASAMRSAQVGSGDRSERIRTYNFPQNRVTDH
RIGLTLYNLDRVMQGELDMITTALVSHAHRQLFGHEETN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=294, Percent_Identity=46.2585034013605, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=40.4191616766467, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=44.6153846153846, Blast_Score=171, Evalue=8e-43,
Organism=Escherichia coli, GI1787462, Length=344, Percent_Identity=54.0697674418605, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=36.4985163204748, Blast_Score=197, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=297, Percent_Identity=37.7104377104377, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=286, Percent_Identity=44.7552447552448, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI19921226, Length=309, Percent_Identity=44.336569579288, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CHLMU (Q9PL16)

Other databases:

- EMBL:   AE002160
- PIR:   B81718
- RefSeq:   NP_296671.1
- ProteinModelPortal:   Q9PL16
- SMR:   Q9PL16
- GeneID:   1246462
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0292
- TIGR:   TC_0292
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q9PL16
- ProtClustDB:   PRK00591
- BioCyc:   CMUR243161:TC_0292-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40208; Mature: 40208

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKVLECLRRLEEVEKLISDPNIFSNPKEYSSLSKEHARLSEIKNAHESILAAKKILHD
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKLALSTEKDPEMIAMLEEGIQGGEESLERLSKQLENLLIPPDPDDDLSVIMELRAGTGG
CCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
DEAALFVGDCVRMYHLYASIKGWQCEVLSASESDLGGYKEYIMGVSGTSVKRFLQYEAGT
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCH
HRVQRVPETETQGRVHTSAVTIAVLPEPAEDDEEVFIDEKDLRIDTFRSSGAGGQHVNVT
HHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCEEEECCCCCCCCCEEEEC
DSAVRITHIPTGVVVSCQDERSQHKNKAKAMRVLKARIRDAEVQKREQEASAMRSAQVGS
CCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDRSERIRTYNFPQNRVTDHRIGLTLYNLDRVMQGELDMITTALVSHAHRQLFGHEETN
CCCCCCCEEECCCCCCCCCHHHCEEEECHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEVKVLECLRRLEEVEKLISDPNIFSNPKEYSSLSKEHARLSEIKNAHESILAAKKILHD
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DKLALSTEKDPEMIAMLEEGIQGGEESLERLSKQLENLLIPPDPDDDLSVIMELRAGTGG
CCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
DEAALFVGDCVRMYHLYASIKGWQCEVLSASESDLGGYKEYIMGVSGTSVKRFLQYEAGT
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCH
HRVQRVPETETQGRVHTSAVTIAVLPEPAEDDEEVFIDEKDLRIDTFRSSGAGGQHVNVT
HHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCEEEECCCCCCCCCEEEEC
DSAVRITHIPTGVVVSCQDERSQHKNKAKAMRVLKARIRDAEVQKREQEASAMRSAQVGS
CCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDRSERIRTYNFPQNRVTDHRIGLTLYNLDRVMQGELDMITTALVSHAHRQLFGHEETN
CCCCCCCEEECCCCCCCCCHHHCEEEECHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935