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The map label for this gene is gatB

Identifier: 15834892

GI number: 15834892

Start: 325825

End: 327291

Strand: Direct

Name: gatB

Synonym: TC0272

Alternate gene names: 15834892

Gene position: 325825-327291 (Clockwise)

Preceding gene: 15834891

Following gene: 15834895

Centisome position: 30.37

GC content: 42.33

Gene sequence:

>1467_bases
ATGGGTATAGCACATACTGAATGGGAGTCTGTGATCGGTCTGGAAGTGCACGTTGAGTTGAATACTGCATCAAAATTGTT
TAGTCCCGCGCGCAATCATTTTGGTGATGAGCCTAATACGAATATTTCCCCTGTATGCACGGGGATGCCGGGAGCTTTAC
CAGTCTTGAATAAGGATGCGGTGCGTAAGGCCGTTTTATTTGGATGTGCTGTCGAAGGGGATGTTGCTTTGTTCAGCCGT
TTTGATAGAAAGTCGTATTTTTACCCAGACAGCCCAAGAAATTTTCAAATTACCCAGTACGAGCATCCTATTGTGAGAGG
CGGGTGTGTGCGTGCTATAGTAGAGGGAGAGGAAAAAACCTTTGAGCTTGCGCAGACGCACTTAGAAGATGATGCTGGGA
TGTTAAAACATTTCGGGGATTTTGCTGGGGTGGATTATAACAGAGCAGGAGTCCCTTTAATTGAGATCGTTTCTAAACCT
TGTATGTTTAGTGCTGAAGATGCCGTGGCTTATGCTAATGCTTTAGTATCTATTCTGAGCTATATCGGTATTTCCGACTG
TAATATGGAGGAAGGATCGGTTCGTTTCGATGTGAACATTTCTGTTCGCCCAAAAGGAAGTAAGGAACTTAGAAATAAGG
TAGAGATTAAAAACATGAACTCCTTTACTTTCATGGCGCAAGCTTTAGAGGCTGAAAAACATCGACAGATTGAGGAGTAT
CTTAGTCATCCAAATGAGGATCCTAAAAAGGTTGTTCCAGCAGCCACCTATCGTTGGGATCCAGAGAAGAAAAAGACAGT
GCTGATGCGTCTTAAGGAGCGCGCTGAAGATTATATGTATTTTGTTGAGCCGGATCTTCCTGTTTTACAAATTACGGAAT
CTTACATTGATGAAGTTCGTCAAACATTGCCAGAGCTGCCTCATAGTAAGTATATGCGTTATATTACAGATTTCGATCTT
GCCGAAGATCTTGCCATGATTCTTGTGAGTGATCGACATACAGCTCATTTTTTTGAAACAGCGACTATGTCTTGTAAGAA
TTATCGCGCCCTTTCTAACTGGATTACAGTTGAATTTGCAGGCCGTTGTAAAGCGACAGGGAAAACGCTCCCATTTACAG
GAATTCTTCCTGAATGGGTGGCGCAACTAGTCAATTTCATAGATCGTGGTGTGATAACAGGAAAAATTGCTAAAGAAATT
GCTGATAAGATGGTTTCTTCTTTCGGAGAAAGTCCAGAAGACATCCTGAGAAGACATCCTTCATTATTACCTATGACTGA
TGATCACGCTTTGCGCGCTATTGTAAAAGAGGTCGTGGCTCAAAATGCAGCATCTGTAGAGGATTACAAAAATGGAAAAG
CGAAAGCTTTGGGCTTTTTAGTTGGGCAGATTATGAAGCGCACAGAAGGGAAAGCTCCTCCTAAGAGAGTGAATGAATTG
TTGTTAGCAGCTATGCGTGATGCATAA

Upstream 100 bases:

>100_bases
CAAATTTGCCAGGTAGGGTATAGTTTCCAAGAACATTCGCAAATCAAACAATTATATCCTAAAGCAGTGAATGGACTTTT
TGACGGAGGAATGGAGTAAA

Downstream 100 bases:

>100_bases
TTGATTGCGATATCGATACAGTGATAAAACCCTCTCCATAAAGTTATGGAGAGGGTTTTTTTATGAATTATAAAACACCA
TTATTTAGGTCGAGACCTGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGIAHTEWESVIGLEVHVELNTASKLFSPARNHFGDEPNTNISPVCTGMPGALPVLNKDAVRKAVLFGCAVEGDVALFSR
FDRKSYFYPDSPRNFQITQYEHPIVRGGCVRAIVEGEEKTFELAQTHLEDDAGMLKHFGDFAGVDYNRAGVPLIEIVSKP
CMFSAEDAVAYANALVSILSYIGISDCNMEEGSVRFDVNISVRPKGSKELRNKVEIKNMNSFTFMAQALEAEKHRQIEEY
LSHPNEDPKKVVPAATYRWDPEKKKTVLMRLKERAEDYMYFVEPDLPVLQITESYIDEVRQTLPELPHSKYMRYITDFDL
AEDLAMILVSDRHTAHFFETATMSCKNYRALSNWITVEFAGRCKATGKTLPFTGILPEWVAQLVNFIDRGVITGKIAKEI
ADKMVSSFGESPEDILRRHPSLLPMTDDHALRAIVKEVVAQNAASVEDYKNGKAKALGFLVGQIMKRTEGKAPPKRVNEL
LLAAMRDA

Sequences:

>Translated_488_residues
MGIAHTEWESVIGLEVHVELNTASKLFSPARNHFGDEPNTNISPVCTGMPGALPVLNKDAVRKAVLFGCAVEGDVALFSR
FDRKSYFYPDSPRNFQITQYEHPIVRGGCVRAIVEGEEKTFELAQTHLEDDAGMLKHFGDFAGVDYNRAGVPLIEIVSKP
CMFSAEDAVAYANALVSILSYIGISDCNMEEGSVRFDVNISVRPKGSKELRNKVEIKNMNSFTFMAQALEAEKHRQIEEY
LSHPNEDPKKVVPAATYRWDPEKKKTVLMRLKERAEDYMYFVEPDLPVLQITESYIDEVRQTLPELPHSKYMRYITDFDL
AEDLAMILVSDRHTAHFFETATMSCKNYRALSNWITVEFAGRCKATGKTLPFTGILPEWVAQLVNFIDRGVITGKIAKEI
ADKMVSSFGESPEDILRRHPSLLPMTDDHALRAIVKEVVAQNAASVEDYKNGKAKALGFLVGQIMKRTEGKAPPKRVNEL
LLAAMRDA
>Mature_487_residues
GIAHTEWESVIGLEVHVELNTASKLFSPARNHFGDEPNTNISPVCTGMPGALPVLNKDAVRKAVLFGCAVEGDVALFSRF
DRKSYFYPDSPRNFQITQYEHPIVRGGCVRAIVEGEEKTFELAQTHLEDDAGMLKHFGDFAGVDYNRAGVPLIEIVSKPC
MFSAEDAVAYANALVSILSYIGISDCNMEEGSVRFDVNISVRPKGSKELRNKVEIKNMNSFTFMAQALEAEKHRQIEEYL
SHPNEDPKKVVPAATYRWDPEKKKTVLMRLKERAEDYMYFVEPDLPVLQITESYIDEVRQTLPELPHSKYMRYITDFDLA
EDLAMILVSDRHTAHFFETATMSCKNYRALSNWITVEFAGRCKATGKTLPFTGILPEWVAQLVNFIDRGVITGKIAKEIA
DKMVSSFGESPEDILRRHPSLLPMTDDHALRAIVKEVVAQNAASVEDYKNGKAKALGFLVGQIMKRTEGKAPPKRVNELL
LAAMRDA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=31.3253012048193, Blast_Score=229, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI115534094, Length=305, Percent_Identity=37.7049180327869, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6319391, Length=513, Percent_Identity=27.8752436647173, Blast_Score=176, Evalue=7e-45,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=30.6122448979592, Blast_Score=221, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CHLMU (Q9PL36)

Other databases:

- EMBL:   AE002160
- PIR:   B81722
- RefSeq:   NP_296651.1
- ProteinModelPortal:   Q9PL36
- SMR:   Q9PL36
- GeneID:   1246442
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0272
- TIGR:   TC_0272
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   CMUR243161:TC_0272-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54760; Mature: 54629

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIAHTEWESVIGLEVHVELNTASKLFSPARNHFGDEPNTNISPVCTGMPGALPVLNKDA
CCCCCCCHHHEEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCHHH
VRKAVLFGCAVEGDVALFSRFDRKSYFYPDSPRNFQITQYEHPIVRGGCVRAIVEGEEKT
HHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCHHH
FELAQTHLEDDAGMLKHFGDFAGVDYNRAGVPLIEIVSKPCMFSAEDAVAYANALVSILS
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
YIGISDCNMEEGSVRFDVNISVRPKGSKELRNKVEIKNMNSFTFMAQALEAEKHRQIEEY
HHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LSHPNEDPKKVVPAATYRWDPEKKKTVLMRLKERAEDYMYFVEPDLPVLQITESYIDEVR
HHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHH
QTLPELPHSKYMRYITDFDLAEDLAMILVSDRHTAHFFETATMSCKNYRALSNWITVEFA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEC
GRCKATGKTLPFTGILPEWVAQLVNFIDRGVITGKIAKEIADKMVSSFGESPEDILRRHP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
SLLPMTDDHALRAIVKEVVAQNAASVEDYKNGKAKALGFLVGQIMKRTEGKAPPKRVNEL
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLAAMRDA
HHHHHHCC
>Mature Secondary Structure 
GIAHTEWESVIGLEVHVELNTASKLFSPARNHFGDEPNTNISPVCTGMPGALPVLNKDA
CCCCCCHHHEEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCHHH
VRKAVLFGCAVEGDVALFSRFDRKSYFYPDSPRNFQITQYEHPIVRGGCVRAIVEGEEKT
HHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCHHH
FELAQTHLEDDAGMLKHFGDFAGVDYNRAGVPLIEIVSKPCMFSAEDAVAYANALVSILS
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
YIGISDCNMEEGSVRFDVNISVRPKGSKELRNKVEIKNMNSFTFMAQALEAEKHRQIEEY
HHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LSHPNEDPKKVVPAATYRWDPEKKKTVLMRLKERAEDYMYFVEPDLPVLQITESYIDEVR
HHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHH
QTLPELPHSKYMRYITDFDLAEDLAMILVSDRHTAHFFETATMSCKNYRALSNWITVEFA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEC
GRCKATGKTLPFTGILPEWVAQLVNFIDRGVITGKIAKEIADKMVSSFGESPEDILRRHP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
SLLPMTDDHALRAIVKEVVAQNAASVEDYKNGKAKALGFLVGQIMKRTEGKAPPKRVNEL
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLAAMRDA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935