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The map label for this gene is gatA

Identifier: 15834891

GI number: 15834891

Start: 324348

End: 325823

Strand: Direct

Name: gatA

Synonym: TC0271

Alternate gene names: 15834891

Gene position: 324348-325823 (Clockwise)

Preceding gene: 15834890

Following gene: 15834892

Centisome position: 30.23

GC content: 41.46

Gene sequence:

>1476_bases
ATGTATCGTAAGAGTGCTTTAGAGTTAAGAGATGCTGTAGTGAATAGAGAAATTTCAGTTACAGCAATAACAGAGTATTT
TTATCATCGTATAGAAAGTTATGATGAACAAATAGGATCTTTTCTTTCTCTCTGTAAAGAAAGGGCGTTACTTAGAGCTT
CACGTATAGATGATAAACTCGCAAAGGGAGATCCAATAGGTATATTAGCCGGGATCCCTATTGGAGTTAAAGATAATATT
CATATCACAGGAGTGAAAACTACCTGCGCATCGAAAATGTTGGAAAACTTTGTAGCTCCTTTTGATGCTACTGTGGTTAG
ACGTATTGAGATGGAAGATGGGATTTTGCTGGGTAAGTTGAACATGGATGAGTTCGCCATGGGATCTACAACTCGGTATT
CGGCTTTCCAACATACTAATAATCCTTGGGACCTGGAGCGTGTCCCTGGGGGATCATCAGGGGGATCAGCAGCTGCAGTT
TCTGCAAGGTTTTGTCCTATTGCTCTAGGATCTGATACAGGTGGATCTATTCGTCAGCCAGCGGCTTTTTGTGGAGTTGT
TGGGTTCAAACCTTCTTATGGAGCCGTTTCTCGCTATGGACTGGTAGCTTTTGGATCTTCCTTGGATCAAATTGGTCCGT
TGACAACTGTTGTTGAAGATGTTGCTTTGGCCATGGATGCTTTTGCTGGTCGCGACATTAAAGACGCAACAACAAGAGAT
TTTTTCAGAGGGACGTTTTCTCAAGCATTATCTCTGGAAGTTCCTAAGTTAATTGGAGTCCCTAGGGGGTTTTTAGATGG
TCTTCAGGAAGACTGTAAAGAAAACTTTTTCGAAGCTCTTGCTGTTATGGAGCGGCAAGGTAGTCGTATCATTGATATAG
ATCTTAGCGTTTTGAAGCATGCAGTTCCTGTGTATTATATTGTTGCTTCAGCGGAAGCTGCTACAAATTTAGCTCGATTT
GATGGAGTTCGCTATGGTCACCGTTGTGCACAGGCGGACAATATGCAAGAAATGTATGCGCGTTCTCGCAAAGAAGGATT
CGGAAAAGAAGTCACTCGAAGAATCCTTTTAGGGAATTATGTTCTTTCTGCAGAAAGACAAAATATTTTTTACAAAAAAG
GAACAGCAGTCCGAGCTACTTTAATAGAAGCTTTTCAATCTGCTTTTGAATGTTGCGATGTGATTGCAATGCCTGTATGT
GCTTCCCCCGCTATTAGAGATACGGATGTTCTTGATCCTGTTTCTCTATATCTACAAGATATTTACACCGTAGCGGTAAA
CCTCGCTTATCTGCCAGCAATTTCAGTTCCTTCAGGGCTTTCTAAGGAAGGACTTCCTTTGGGAGTGCAATTTATCGGGA
AAAGAGGAGCGGATCAGCAAATTTGCCAGGTAGGGTATAGTTTCCAAGAACATTCGCAAATCAAACAATTATATCCTAAA
GCAGTGAATGGACTTTTTGACGGAGGAATGGAGTAA

Upstream 100 bases:

>100_bases
TAACTTCAGATTTTACCCGAGAAGAATTTTTAGCTAATGTTCCTGTGTCTTTAGGAGGATTAGTCAAAGTCCCTACAGTT
ATCAAATAAGGTGGATCGCT

Downstream 100 bases:

>100_bases
AATGGGTATAGCACATACTGAATGGGAGTCTGTGATCGGTCTGGAAGTGCACGTTGAGTTGAATACTGCATCAAAATTGT
TTAGTCCCGCGCGCAATCAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MYRKSALELRDAVVNREISVTAITEYFYHRIESYDEQIGSFLSLCKERALLRASRIDDKLAKGDPIGILAGIPIGVKDNI
HITGVKTTCASKMLENFVAPFDATVVRRIEMEDGILLGKLNMDEFAMGSTTRYSAFQHTNNPWDLERVPGGSSGGSAAAV
SARFCPIALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDAFAGRDIKDATTRD
FFRGTFSQALSLEVPKLIGVPRGFLDGLQEDCKENFFEALAVMERQGSRIIDIDLSVLKHAVPVYYIVASAEAATNLARF
DGVRYGHRCAQADNMQEMYARSRKEGFGKEVTRRILLGNYVLSAERQNIFYKKGTAVRATLIEAFQSAFECCDVIAMPVC
ASPAIRDTDVLDPVSLYLQDIYTVAVNLAYLPAISVPSGLSKEGLPLGVQFIGKRGADQQICQVGYSFQEHSQIKQLYPK
AVNGLFDGGME

Sequences:

>Translated_491_residues
MYRKSALELRDAVVNREISVTAITEYFYHRIESYDEQIGSFLSLCKERALLRASRIDDKLAKGDPIGILAGIPIGVKDNI
HITGVKTTCASKMLENFVAPFDATVVRRIEMEDGILLGKLNMDEFAMGSTTRYSAFQHTNNPWDLERVPGGSSGGSAAAV
SARFCPIALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDAFAGRDIKDATTRD
FFRGTFSQALSLEVPKLIGVPRGFLDGLQEDCKENFFEALAVMERQGSRIIDIDLSVLKHAVPVYYIVASAEAATNLARF
DGVRYGHRCAQADNMQEMYARSRKEGFGKEVTRRILLGNYVLSAERQNIFYKKGTAVRATLIEAFQSAFECCDVIAMPVC
ASPAIRDTDVLDPVSLYLQDIYTVAVNLAYLPAISVPSGLSKEGLPLGVQFIGKRGADQQICQVGYSFQEHSQIKQLYPK
AVNGLFDGGME
>Mature_491_residues
MYRKSALELRDAVVNREISVTAITEYFYHRIESYDEQIGSFLSLCKERALLRASRIDDKLAKGDPIGILAGIPIGVKDNI
HITGVKTTCASKMLENFVAPFDATVVRRIEMEDGILLGKLNMDEFAMGSTTRYSAFQHTNNPWDLERVPGGSSGGSAAAV
SARFCPIALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDAFAGRDIKDATTRD
FFRGTFSQALSLEVPKLIGVPRGFLDGLQEDCKENFFEALAVMERQGSRIIDIDLSVLKHAVPVYYIVASAEAATNLARF
DGVRYGHRCAQADNMQEMYARSRKEGFGKEVTRRILLGNYVLSAERQNIFYKKGTAVRATLIEAFQSAFECCDVIAMPVC
ASPAIRDTDVLDPVSLYLQDIYTVAVNLAYLPAISVPSGLSKEGLPLGVQFIGKRGADQQICQVGYSFQEHSQIKQLYPK
AVNGLFDGGME

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=36, Blast_Score=305, Evalue=9e-83,
Organism=Homo sapiens, GI195972892, Length=241, Percent_Identity=29.4605809128631, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=453, Percent_Identity=35.0993377483444, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17537465, Length=485, Percent_Identity=25.1546391752577, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556264, Length=184, Percent_Identity=31.5217391304348, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=200, Percent_Identity=29.5, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=189, Percent_Identity=30.1587301587302, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=189, Percent_Identity=30.1587301587302, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=191, Percent_Identity=29.3193717277487, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=416, Percent_Identity=33.8942307692308, Blast_Score=216, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6319685, Length=438, Percent_Identity=28.0821917808219, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24648113, Length=466, Percent_Identity=36.2660944206009, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI45550774, Length=484, Percent_Identity=25, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648435, Length=484, Percent_Identity=25, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648437, Length=484, Percent_Identity=25, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=23.2741617357002, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648441, Length=414, Percent_Identity=25.6038647342995, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648439, Length=414, Percent_Identity=25.6038647342995, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=24.8971193415638, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=24.8971193415638, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=24.8971193415638, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=24.8971193415638, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078093, Length=493, Percent_Identity=24.5436105476673, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21356731, Length=244, Percent_Identity=30.7377049180328, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CHLMU (Q9PL37)

Other databases:

- EMBL:   AE002160
- PIR:   A81722
- RefSeq:   NP_296650.1
- ProteinModelPortal:   Q9PL37
- SMR:   Q9PL37
- GeneID:   1246441
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0271
- TIGR:   TC_0271
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- PhylomeDB:   Q9PL37
- ProtClustDB:   PRK00012
- BioCyc:   CMUR243161:TC_0271-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53544; Mature: 53544

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRKSALELRDAVVNREISVTAITEYFYHRIESYDEQIGSFLSLCKERALLRASRIDDKL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGDPIGILAGIPIGVKDNIHITGVKTTCASKMLENFVAPFDATVVRRIEMEDGILLGKL
HCCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHEECCCCEEEEEC
NMDEFAMGSTTRYSAFQHTNNPWDLERVPGGSSGGSAAAVSARFCPIALGSDTGGSIRQP
CCHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCC
AAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDAFAGRDIKDATTRD
HHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FFRGTFSQALSLEVPKLIGVPRGFLDGLQEDCKENFFEALAVMERQGSRIIDIDLSVLKH
HHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHH
AVPVYYIVASAEAATNLARFDGVRYGHRCAQADNMQEMYARSRKEGFGKEVTRRILLGNY
CCCCEEEEECHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
VLSAERQNIFYKKGTAVRATLIEAFQSAFECCDVIAMPVCASPAIRDTDVLDPVSLYLQD
HEEHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
IYTVAVNLAYLPAISVPSGLSKEGLPLGVQFIGKRGADQQICQVGYSFQEHSQIKQLYPK
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHH
AVNGLFDGGME
HHHHHHCCCCC
>Mature Secondary Structure
MYRKSALELRDAVVNREISVTAITEYFYHRIESYDEQIGSFLSLCKERALLRASRIDDKL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGDPIGILAGIPIGVKDNIHITGVKTTCASKMLENFVAPFDATVVRRIEMEDGILLGKL
HCCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHEECCCCEEEEEC
NMDEFAMGSTTRYSAFQHTNNPWDLERVPGGSSGGSAAAVSARFCPIALGSDTGGSIRQP
CCHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCC
AAFCGVVGFKPSYGAVSRYGLVAFGSSLDQIGPLTTVVEDVALAMDAFAGRDIKDATTRD
HHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FFRGTFSQALSLEVPKLIGVPRGFLDGLQEDCKENFFEALAVMERQGSRIIDIDLSVLKH
HHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHH
AVPVYYIVASAEAATNLARFDGVRYGHRCAQADNMQEMYARSRKEGFGKEVTRRILLGNY
CCCCEEEEECHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
VLSAERQNIFYKKGTAVRATLIEAFQSAFECCDVIAMPVCASPAIRDTDVLDPVSLYLQD
HEEHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
IYTVAVNLAYLPAISVPSGLSKEGLPLGVQFIGKRGADQQICQVGYSFQEHSQIKQLYPK
HHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHH
AVNGLFDGGME
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935