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The map label for this gene is glgB

Identifier: 15834877

GI number: 15834877

Start: 299702

End: 301915

Strand: Direct

Name: glgB

Synonym: TC0257

Alternate gene names: 15834877

Gene position: 299702-301915 (Clockwise)

Preceding gene: 15834876

Following gene: 15834883

Centisome position: 27.93

GC content: 41.24

Gene sequence:

>2214_bases
GTGGATCCTTTCTTCTTAAATACACAACATGTAGAGCTTCTTGTTTCAGGGAAACAGAGTAGCCCGCAAGATCTTTTAGG
AATTGTTTCTGAAAGTCTGAATCAGGATCGGATAGTTCTTTTTCGTCCTGGAGCAAAGACTGTTTTTGTTGAGCTGCAAG
GAAAGATTCAGCAAGCAGAATCGCATCATTCAGGGATTTTTTCTCTCCCTGTCACGAAAGGAATTTCTCCTCAAGACTAT
CGAGTGTATCATCAAAATGGTCTATTAGCTCATGATCCCTATGCATTTCCTTTGCTTTGGGGAGAAATCGATTCTTTTTT
ATTCCATGAAGGGACCCATCAGCATATCTATGATCGCATGGGTGCTATTCCTTGTGAAATTGGAGGGGTGCCTGGAGTGC
GGTTTGTTATTTGGGCTCCGCATGCTCAAAGAGTTTCTGTTGTTGGAGATTTTAACTGCTGGCATGGACTTGTTAACCCT
CTTCATAAAGTTTCAGATCAAGGAGTTTGGGAGTTATTTGTTCCTGGCCTCACGGCAGGTTCTTGTTATAAGTGGGAAAT
AGTTACAGCATCTGGTCAGGTTCTTATCAAATCTGATCCTTATGGTAAATTTTTTGGATCTCCTCCGCAAAGCGTTTCTG
TTGTTGTGGATGATCGCTATGAGTGGGGAGATAAAGAGTGGTTAGACGAGCGCTCTAAAAAAGCCGAAGGCCCTATGAAT
ATTTATGAAGTGCATGTAGGGTCTTGGAGATGGCAAGAAGAGCAGCCTTTAAATTATAGAGAGTTAGCTGATCAACTGTC
CCTCTATTGTAAGCAGATGCATTATACGCATGTGGAGTTGCTCCCTGTAACAGAGCATCCTTTGAATGAATCTTGGGGAT
ATCAAACAACAGGATATTATGCTCCAACTAGTCGTTACGGTTCTTTTGAGGATTTACAGTATTTTATAGATACCATGCAT
CAGCATGGTATTGGGGTTATTTTGGACTGGGTTCCGGGACATTTCCCTGTAGATTCTTTTGCAATGAGTAATTTTGATGG
TACACCATTATACGAGTATACTAGAAATCCTAGTCCATTGCATCCTCATTGGCATACATATACCTTTGATTATGCCAAGC
CTGAGGTTTGTAATTTCTTATTAGGGAGTGCTCTATTTTGGATAGATAAAATGCATATAGATGGTATCCGAGTCGATGCT
GTTTCTTCCATGCTTTATCTTGATTATGGACGAAATCCAGGGGAGTGGATTCCGAATCGCTATGGGGGAAGAGAAAACCT
TGATGCAGTCCGTTTTCTTCAGCAATTTAACACAATCATTCATGAGAAGTATCCAGGTGTCTTGACCTTTGCTGAAGAGT
CTACAACATTCCCCAAGATCACTGTGTCTGTGGAAGAAGGAGGATTAGGTTTTGATTATAAATGGAATATGGGCTGGATG
CATGATACATTGCATTATTTTGAAAAAGATTTTCCTTACCGGCCATATCATCAAAGCGATCTGACTTTCCCTCAGTGGTA
TGCCTTTAGTGAAAGGTTCTTGCTACCATTTTCTCATGACGAGGTAGTTCATGGGAAACGGAGTTTAATTGGTAAAATGC
CTGGGGATGCCTGGAGACAGTTCGCTCAGTTACGTTTGCTATTGGGGTATCAAATCTGTCAACCAGGGAAGAAATTGCTC
TTTATGGGCGGAGAATTTGGGCAAGGACGAGAGTGGTCGCCGAATCGCGAATTGGATTGGGATTTATTGGATATCCATTA
CCATCAGGGAGTACATTTATGCTCTCAAAAGTTAAACTCCTTATATGTGAATAGCCCTCAGTTGTGGAAAGGAGATCATT
TACCACAGAGTTTTCGTTGGATAGATTTTAGTGATACTCGTAATGGTGTTGTTGCTTATTTGCGATTTGCTTCTGATGAG
GATAAGGAAGCATTGCTTTGCGTGCATCATTTTGGCGTGAATCATTTCCTTCATTACATTTTACCGATGCTCCCTATGAA
ATCGTGTTCTTTGCTTATGAATACAGACGATTTAGCGTTTGGGGGATCGGGCAAAGGTTTTCGAAATCCCAAAATGTTAA
CTCCTGAAATGGTTAGAAAAGAGAAAACCTCTTCAGAACTAGCTCCTCTGGATGATGATGGTAGCGTTGCTTGGGGATTA
GATATCGAGATGCCTCCGTCAGCAACGCTCATTTTTTCTGTAACACTACAATAA

Upstream 100 bases:

>100_bases
TTATCTCTTGTAAAATTAAAAAAAATTTAGAAAAGGTGGAGATAAAAGCGGGAACCACGTATCTATGGGACCGTTTTTGT
AAATAAACGCAGAAAATGTC

Downstream 100 bases:

>100_bases
TGTTAATGTGATGGCCAGTTTAAAGCGGAATATTTGGACATGGATGCTTGGGATGCAGCGCAAAATCTAGTGAGCATAGA
TTTTCTATCGCATACGCTTA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MDPFFLNTQHVELLVSGKQSSPQDLLGIVSESLNQDRIVLFRPGAKTVFVELQGKIQQAESHHSGIFSLPVTKGISPQDY
RVYHQNGLLAHDPYAFPLLWGEIDSFLFHEGTHQHIYDRMGAIPCEIGGVPGVRFVIWAPHAQRVSVVGDFNCWHGLVNP
LHKVSDQGVWELFVPGLTAGSCYKWEIVTASGQVLIKSDPYGKFFGSPPQSVSVVVDDRYEWGDKEWLDERSKKAEGPMN
IYEVHVGSWRWQEEQPLNYRELADQLSLYCKQMHYTHVELLPVTEHPLNESWGYQTTGYYAPTSRYGSFEDLQYFIDTMH
QHGIGVILDWVPGHFPVDSFAMSNFDGTPLYEYTRNPSPLHPHWHTYTFDYAKPEVCNFLLGSALFWIDKMHIDGIRVDA
VSSMLYLDYGRNPGEWIPNRYGGRENLDAVRFLQQFNTIIHEKYPGVLTFAEESTTFPKITVSVEEGGLGFDYKWNMGWM
HDTLHYFEKDFPYRPYHQSDLTFPQWYAFSERFLLPFSHDEVVHGKRSLIGKMPGDAWRQFAQLRLLLGYQICQPGKKLL
FMGGEFGQGREWSPNRELDWDLLDIHYHQGVHLCSQKLNSLYVNSPQLWKGDHLPQSFRWIDFSDTRNGVVAYLRFASDE
DKEALLCVHHFGVNHFLHYILPMLPMKSCSLLMNTDDLAFGGSGKGFRNPKMLTPEMVRKEKTSSELAPLDDDGSVAWGL
DIEMPPSATLIFSVTLQ

Sequences:

>Translated_737_residues
MDPFFLNTQHVELLVSGKQSSPQDLLGIVSESLNQDRIVLFRPGAKTVFVELQGKIQQAESHHSGIFSLPVTKGISPQDY
RVYHQNGLLAHDPYAFPLLWGEIDSFLFHEGTHQHIYDRMGAIPCEIGGVPGVRFVIWAPHAQRVSVVGDFNCWHGLVNP
LHKVSDQGVWELFVPGLTAGSCYKWEIVTASGQVLIKSDPYGKFFGSPPQSVSVVVDDRYEWGDKEWLDERSKKAEGPMN
IYEVHVGSWRWQEEQPLNYRELADQLSLYCKQMHYTHVELLPVTEHPLNESWGYQTTGYYAPTSRYGSFEDLQYFIDTMH
QHGIGVILDWVPGHFPVDSFAMSNFDGTPLYEYTRNPSPLHPHWHTYTFDYAKPEVCNFLLGSALFWIDKMHIDGIRVDA
VSSMLYLDYGRNPGEWIPNRYGGRENLDAVRFLQQFNTIIHEKYPGVLTFAEESTTFPKITVSVEEGGLGFDYKWNMGWM
HDTLHYFEKDFPYRPYHQSDLTFPQWYAFSERFLLPFSHDEVVHGKRSLIGKMPGDAWRQFAQLRLLLGYQICQPGKKLL
FMGGEFGQGREWSPNRELDWDLLDIHYHQGVHLCSQKLNSLYVNSPQLWKGDHLPQSFRWIDFSDTRNGVVAYLRFASDE
DKEALLCVHHFGVNHFLHYILPMLPMKSCSLLMNTDDLAFGGSGKGFRNPKMLTPEMVRKEKTSSELAPLDDDGSVAWGL
DIEMPPSATLIFSVTLQ
>Mature_737_residues
MDPFFLNTQHVELLVSGKQSSPQDLLGIVSESLNQDRIVLFRPGAKTVFVELQGKIQQAESHHSGIFSLPVTKGISPQDY
RVYHQNGLLAHDPYAFPLLWGEIDSFLFHEGTHQHIYDRMGAIPCEIGGVPGVRFVIWAPHAQRVSVVGDFNCWHGLVNP
LHKVSDQGVWELFVPGLTAGSCYKWEIVTASGQVLIKSDPYGKFFGSPPQSVSVVVDDRYEWGDKEWLDERSKKAEGPMN
IYEVHVGSWRWQEEQPLNYRELADQLSLYCKQMHYTHVELLPVTEHPLNESWGYQTTGYYAPTSRYGSFEDLQYFIDTMH
QHGIGVILDWVPGHFPVDSFAMSNFDGTPLYEYTRNPSPLHPHWHTYTFDYAKPEVCNFLLGSALFWIDKMHIDGIRVDA
VSSMLYLDYGRNPGEWIPNRYGGRENLDAVRFLQQFNTIIHEKYPGVLTFAEESTTFPKITVSVEEGGLGFDYKWNMGWM
HDTLHYFEKDFPYRPYHQSDLTFPQWYAFSERFLLPFSHDEVVHGKRSLIGKMPGDAWRQFAQLRLLLGYQICQPGKKLL
FMGGEFGQGREWSPNRELDWDLLDIHYHQGVHLCSQKLNSLYVNSPQLWKGDHLPQSFRWIDFSDTRNGVVAYLRFASDE
DKEALLCVHHFGVNHFLHYILPMLPMKSCSLLMNTDDLAFGGSGKGFRNPKMLTPEMVRKEKTSSELAPLDDDGSVAWGL
DIEMPPSATLIFSVTLQ

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=431, Percent_Identity=30.1624129930394, Blast_Score=175, Evalue=1e-43,
Organism=Escherichia coli, GI1789839, Length=690, Percent_Identity=43.4782608695652, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI2367229, Length=228, Percent_Identity=27.6315789473684, Blast_Score=63, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17554896, Length=357, Percent_Identity=32.4929971988796, Blast_Score=182, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI32564391, Length=329, Percent_Identity=31.9148936170213, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320826, Length=373, Percent_Identity=29.7587131367292, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI28573410, Length=359, Percent_Identity=34.2618384401114, Blast_Score=179, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_CHLMU (Q9PL51)

Other databases:

- EMBL:   AE002160
- PIR:   C81724
- RefSeq:   NP_296636.1
- ProteinModelPortal:   Q9PL51
- SMR:   Q9PL51
- GeneID:   1246427
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0257
- TIGR:   TC_0257
- HOGENOM:   HBG287139
- OMA:   RVYHQNG
- PhylomeDB:   Q9PL51
- ProtClustDB:   PRK05402
- BioCyc:   CMUR243161:TC_0257-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 84640; Mature: 84640

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: ACT_SITE 294-294 ACT_SITE 329-329 ACT_SITE 334-334 ACT_SITE 397-397 ACT_SITE 399-399 ACT_SITE 452-452 ACT_SITE 519-519 ACT_SITE 520-520

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPFFLNTQHVELLVSGKQSSPQDLLGIVSESLNQDRIVLFRPGAKTVFVELQGKIQQAE
CCCCEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHH
SHHSGIFSLPVTKGISPQDYRVYHQNGLLAHDPYAFPLLWGEIDSFLFHEGTHQHIYDRM
HHCCCEEEECCCCCCCCCCEEEEECCCCEEECCCEEEEEHHHHHHHHHCCCCHHHHHHHH
GAIPCEIGGVPGVRFVIWAPHAQRVSVVGDFNCWHGLVNPLHKVSDQGVWELFVPGLTAG
CCCCEECCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCC
SCYKWEIVTASGQVLIKSDPYGKFFGSPPQSVSVVVDDRYEWGDKEWLDERSKKAEGPMN
CEEEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCE
IYEVHVGSWRWQEEQPLNYRELADQLSLYCKQMHYTHVELLPVTEHPLNESWGYQTTGYY
EEEEEECCEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEE
APTSRYGSFEDLQYFIDTMHQHGIGVILDWVPGHFPVDSFAMSNFDGTPLYEYTRNPSPL
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHCCCCCCCCHHCCCCCCCC
HPHWHTYTFDYAKPEVCNFLLGSALFWIDKMHIDGIRVDAVSSMLYLDYGRNPGEWIPNR
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEECCEEHHHHHEEEEEECCCCCCCCCCCC
YGGRENLDAVRFLQQFNTIIHEKYPGVLTFAEESTTFPKITVSVEEGGLGFDYKWNMGWM
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEECCCHH
HDTLHYFEKDFPYRPYHQSDLTFPQWYAFSERFLLPFSHDEVVHGKRSLIGKMPGDAWRQ
HHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHCCHHHHHCCCCCHHHHH
FAQLRLLLGYQICQPGKKLLFMGGEFGQGREWSPNRELDWDLLDIHYHQGVHLCSQKLNS
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEHHCCHHHHHHHHHH
LYVNSPQLWKGDHLPQSFRWIDFSDTRNGVVAYLRFASDEDKEALLCVHHFGVNHFLHYI
EECCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEEEHHCHHHHHHHH
LPMLPMKSCSLLMNTDDLAFGGSGKGFRNPKMLTPEMVRKEKTSSELAPLDDDGSVAWGL
HHHCCCCCCEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEE
DIEMPPSATLIFSVTLQ
EECCCCCCEEEEEEEEC
>Mature Secondary Structure
MDPFFLNTQHVELLVSGKQSSPQDLLGIVSESLNQDRIVLFRPGAKTVFVELQGKIQQAE
CCCCEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHH
SHHSGIFSLPVTKGISPQDYRVYHQNGLLAHDPYAFPLLWGEIDSFLFHEGTHQHIYDRM
HHCCCEEEECCCCCCCCCCEEEEECCCCEEECCCEEEEEHHHHHHHHHCCCCHHHHHHHH
GAIPCEIGGVPGVRFVIWAPHAQRVSVVGDFNCWHGLVNPLHKVSDQGVWELFVPGLTAG
CCCCEECCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCC
SCYKWEIVTASGQVLIKSDPYGKFFGSPPQSVSVVVDDRYEWGDKEWLDERSKKAEGPMN
CEEEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCE
IYEVHVGSWRWQEEQPLNYRELADQLSLYCKQMHYTHVELLPVTEHPLNESWGYQTTGYY
EEEEEECCEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEE
APTSRYGSFEDLQYFIDTMHQHGIGVILDWVPGHFPVDSFAMSNFDGTPLYEYTRNPSPL
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHCCCCCCCCHHCCCCCCCC
HPHWHTYTFDYAKPEVCNFLLGSALFWIDKMHIDGIRVDAVSSMLYLDYGRNPGEWIPNR
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEECCEEHHHHHEEEEEECCCCCCCCCCCC
YGGRENLDAVRFLQQFNTIIHEKYPGVLTFAEESTTFPKITVSVEEGGLGFDYKWNMGWM
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEECCCHH
HDTLHYFEKDFPYRPYHQSDLTFPQWYAFSERFLLPFSHDEVVHGKRSLIGKMPGDAWRQ
HHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHCCHHHHHCCCCCHHHHH
FAQLRLLLGYQICQPGKKLLFMGGEFGQGREWSPNRELDWDLLDIHYHQGVHLCSQKLNS
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEHHCCHHHHHHHHHH
LYVNSPQLWKGDHLPQSFRWIDFSDTRNGVVAYLRFASDEDKEALLCVHHFGVNHFLHYI
EECCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEEEHHCHHHHHHHH
LPMLPMKSCSLLMNTDDLAFGGSGKGFRNPKMLTPEMVRKEKTSSELAPLDDDGSVAWGL
HHHCCCCCCEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEE
DIEMPPSATLIFSVTLQ
EECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935