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The map label for this gene is ispH

Identifier: 15834869

GI number: 15834869

Start: 290704

End: 291630

Strand: Direct

Name: ispH

Synonym: TC0249

Alternate gene names: 15834869

Gene position: 290704-291630 (Clockwise)

Preceding gene: 15834868

Following gene: 15834874

Centisome position: 27.09

GC content: 38.73

Gene sequence:

>927_bases
ATGCGTAAGGTTATCATCTGTTCTCCTAGAGGTTTTTGTGCTGGAGTCATTCGCGCGGTACAAACTGTAGAAAGAGCTTT
AGAGAAATGGGGAGCTCCTATTTATGTTAAGCATGAGATTGTTCATAATCGTCATGTTGTGGATAAGCTACGGGCTAAAG
GAGCTATTTTTATTGAAGATTTACAAGAAGTCCCTTGCAATAGTCGAGTTATTTATTCAGCGCATGGTGTTCCCCCTTCT
GTAAGGGAAGAAGCTAAAGAAAGGGGACTAATTACTATTGATGCAACATGTGGTCTTGTTACAAAAATTCATTCCGCGGT
CAAAATGTATGCTCGGAGGGGATACCTTATTATTCTCATAGGTAAGAGAAAACATGTTGAGGTTATCGGGATATGTGGTG
AAGCACCTGATAAAATTACTGTTGTGGAAAATATCGCTGAAGTAGAGGCGCTGACTTTTAGTACGAAGGATCTTTTGTTC
TATGTGACGCAAACGACTTTAAGTATGGATGATTCTGCAGATATGATCGCTGCTTTAAAAGCTCGTTATCCTCAAATTAT
TACATTACCAAGTTCTTCTATCTGTTATGCTACGCAAAACCGTCAGAGCGCTTTACGCAATATTCTTCCGAAGGTGGAAT
TTGTTTATGTAATAGGGGATCCTAAAAGTTCCAATTCAAATCAGTTAAAAGCAGTTGCTGCAAGACGCGGTGTTGTAGCT
CGGCTTGTAAATAATCCTGAAGAAATTACGGATGAAATTTTACAATATTCTGGGAATATTGGTGTGACAGCAGGAGCTTC
GACTCCTGAAGATGTTGTACAGGCTTGTTTAACAAAATTACAGAAACTCATTCCAACTTTAGTTGTAGAAACGGATCTTT
TTGTAGAAGAAGATACCATATTTCAACTCCCTAAAGAATTGCAGTGA

Upstream 100 bases:

>100_bases
TAACTGTTATGTTTATTTGTAAGTTAAGTAAGGGCTTTTCGACTTTGTGTCGAGAGGCCCTTATTTTGTTTAAAATGTTA
ACGAAAGGAGGAGATAGTGC

Downstream 100 bases:

>100_bases
AATCAATAAGATGAAGAAACTTCATCTTATTGATAAAGACTGCGGGCAGCATCATGTTGTGTTTGTAGAATTTGTAGTAA
GAAGCTGTCCATATTTCTCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRKVIICSPRGFCAGVIRAVQTVERALEKWGAPIYVKHEIVHNRHVVDKLRAKGAIFIEDLQEVPCNSRVIYSAHGVPPS
VREEAKERGLITIDATCGLVTKIHSAVKMYARRGYLIILIGKRKHVEVIGICGEAPDKITVVENIAEVEALTFSTKDLLF
YVTQTTLSMDDSADMIAALKARYPQIITLPSSSICYATQNRQSALRNILPKVEFVYVIGDPKSSNSNQLKAVAARRGVVA
RLVNNPEEITDEILQYSGNIGVTAGASTPEDVVQACLTKLQKLIPTLVVETDLFVEEDTIFQLPKELQ

Sequences:

>Translated_308_residues
MRKVIICSPRGFCAGVIRAVQTVERALEKWGAPIYVKHEIVHNRHVVDKLRAKGAIFIEDLQEVPCNSRVIYSAHGVPPS
VREEAKERGLITIDATCGLVTKIHSAVKMYARRGYLIILIGKRKHVEVIGICGEAPDKITVVENIAEVEALTFSTKDLLF
YVTQTTLSMDDSADMIAALKARYPQIITLPSSSICYATQNRQSALRNILPKVEFVYVIGDPKSSNSNQLKAVAARRGVVA
RLVNNPEEITDEILQYSGNIGVTAGASTPEDVVQACLTKLQKLIPTLVVETDLFVEEDTIFQLPKELQ
>Mature_308_residues
MRKVIICSPRGFCAGVIRAVQTVERALEKWGAPIYVKHEIVHNRHVVDKLRAKGAIFIEDLQEVPCNSRVIYSAHGVPPS
VREEAKERGLITIDATCGLVTKIHSAVKMYARRGYLIILIGKRKHVEVIGICGEAPDKITVVENIAEVEALTFSTKDLLF
YVTQTTLSMDDSADMIAALKARYPQIITLPSSSICYATQNRQSALRNILPKVEFVYVIGDPKSSNSNQLKAVAARRGVVA
RLVNNPEEITDEILQYSGNIGVTAGASTPEDVVQACLTKLQKLIPTLVVETDLFVEEDTIFQLPKELQ

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=46.2783171521036, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CHLMU (Q9PL59)

Other databases:

- EMBL:   AE002160
- PIR:   C81723
- RefSeq:   NP_296628.1
- GeneID:   1246418
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0249
- TIGR:   TC_0249
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q9PL59
- ProtClustDB:   PRK01045
- BioCyc:   CMUR243161:TC_0249-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34010; Mature: 34010

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVIICSPRGFCAGVIRAVQTVERALEKWGAPIYVKHEIVHNRHVVDKLRAKGAIFIED
CCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEH
LQEVPCNSRVIYSAHGVPPSVREEAKERGLITIDATCGLVTKIHSAVKMYARRGYLIILI
HHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEEE
GKRKHVEVIGICGEAPDKITVVENIAEVEALTFSTKDLLFYVTQTTLSMDDSADMIAALK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHEECCHHEEEEEEEHEECCCCCHHHHHHHH
ARYPQIITLPSSSICYATQNRQSALRNILPKVEFVYVIGDPKSSNSNQLKAVAARRGVVA
HCCCEEEECCCCCEEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
RLVNNPEEITDEILQYSGNIGVTAGASTPEDVVQACLTKLQKLIPTLVVETDLFVEEDTI
HHHCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECHHEECCCHH
FQLPKELQ
HHCHHHCC
>Mature Secondary Structure
MRKVIICSPRGFCAGVIRAVQTVERALEKWGAPIYVKHEIVHNRHVVDKLRAKGAIFIED
CCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEH
LQEVPCNSRVIYSAHGVPPSVREEAKERGLITIDATCGLVTKIHSAVKMYARRGYLIILI
HHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEEE
GKRKHVEVIGICGEAPDKITVVENIAEVEALTFSTKDLLFYVTQTTLSMDDSADMIAALK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHEECCHHEEEEEEEHEECCCCCHHHHHHHH
ARYPQIITLPSSSICYATQNRQSALRNILPKVEFVYVIGDPKSSNSNQLKAVAARRGVVA
HCCCEEEECCCCCEEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
RLVNNPEEITDEILQYSGNIGVTAGASTPEDVVQACLTKLQKLIPTLVVETDLFVEEDTI
HHHCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECHHEECCCHH
FQLPKELQ
HHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935