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Identifier: 15834848

GI number: 15834848

Start: 267918

End: 268883

Strand: Direct

Name: Not Available

Synonym: TC0228

Alternate gene names: 15834848

Gene position: 267918-268883 (Clockwise)

Preceding gene: 15834845

Following gene: 15834849

Centisome position: 24.97

GC content: 39.75

Gene sequence:

>966_bases
ATGGTAATCCGTTTATTCGAGAATGATAAGCAATTAGAAGTTTTTTTTTCTTCTTTAGACAAGAAAAAAAAATACTTGCT
GGCGTTATCCGGCGGGAGTGATTCCTTATTATTAATGTATTTGCTGAGGTCTCGAGGCATTTCTTTCACGGCTGTACATG
TGGATTATGGCTGGAGGGAGACTTCATATCAGGAAGCTTGTGATTTGGCTTCCTTATGCGAGCGGGAACAAATTCCTTTT
ATTTTAGATCGCCAGGAAGTCGCAAATCCTATGGATTTTAGTGACATCGAGAATATTGCTCGGCAATACCGTTACGAGTT
ATTTTATCGATTGTGTAAAGAACGACTTTTCGCAGGTGTGTTTCTAGGGCATCATGCAGATGATCAAGCAGAAACCATTT
TGAAACGGGTATTTGAAGGAGCTCATCTTGGAAATCTCAAGGGAATGGCACGGTATGGAACATATAAGGGCATAACATTA
CTGCGACCGCTTCTACATATTACTAAGAGGCAAATTGTTGAAGCTCTTGATAATTATCGAATTGAGTATGTTCAGGATGC
CACAAATGCTGATGAACGCTTTTTACGTGCAAGAATGAGGGAACAATTATTCCCTTATCTACAAGAGATTTTCGGGAAAA
ATATCAGACAGCCTTTGCTTTTTCTTGCGGAAGATTCTGCAGAGCTCAGAGAGTACTTAGATCAGCAAGCAGCACCTTTT
TTATCGCAGGTTATTGATAATGAGATAGGACAATTTCTACCTGTGGGACAAGAGTTATTACAAACGGCCTTTTTGACAAA
GTGGGTATGTAAGCAGTTTTTCTTCAAGCAGGGGCTTGTCGCTTCAAAGGGTTTTTTGCAAACGGTTTATGATCATCTCG
TGCGAAGATCTGAGGCAAGATTACGACTTCGTAATCGAACCGTATTGGTAAAAGCTAGGGGAGTAATCATAGAAAGTATA
TATTGA

Upstream 100 bases:

>100_bases
CATGGCGATTCCGCAATAGACAATTAGTTAAACGTTTTAGTTAACAAAACTACGCGCATAGAAAAATCTTGTAAATCTGT
GGATAGTTAAGAACGCTGTT

Downstream 100 bases:

>100_bases
TGGTGTTTTCAGGGCTTTCATAGTATGGTTCATCAGATTGTTGAAAGAAAAACACTTCTTGTTTTTTATCTCTACAATTA
GAGGTTTATAAACAAAATAA

Product: MesJ/Ycf62 family protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVIRLFENDKQLEVFFSSLDKKKKYLLALSGGSDSLLLMYLLRSRGISFTAVHVDYGWRETSYQEACDLASLCEREQIPF
ILDRQEVANPMDFSDIENIARQYRYELFYRLCKERLFAGVFLGHHADDQAETILKRVFEGAHLGNLKGMARYGTYKGITL
LRPLLHITKRQIVEALDNYRIEYVQDATNADERFLRARMREQLFPYLQEIFGKNIRQPLLFLAEDSAELREYLDQQAAPF
LSQVIDNEIGQFLPVGQELLQTAFLTKWVCKQFFFKQGLVASKGFLQTVYDHLVRRSEARLRLRNRTVLVKARGVIIESI
Y

Sequences:

>Translated_321_residues
MVIRLFENDKQLEVFFSSLDKKKKYLLALSGGSDSLLLMYLLRSRGISFTAVHVDYGWRETSYQEACDLASLCEREQIPF
ILDRQEVANPMDFSDIENIARQYRYELFYRLCKERLFAGVFLGHHADDQAETILKRVFEGAHLGNLKGMARYGTYKGITL
LRPLLHITKRQIVEALDNYRIEYVQDATNADERFLRARMREQLFPYLQEIFGKNIRQPLLFLAEDSAELREYLDQQAAPF
LSQVIDNEIGQFLPVGQELLQTAFLTKWVCKQFFFKQGLVASKGFLQTVYDHLVRRSEARLRLRNRTVLVKARGVIIESI
Y
>Mature_321_residues
MVIRLFENDKQLEVFFSSLDKKKKYLLALSGGSDSLLLMYLLRSRGISFTAVHVDYGWRETSYQEACDLASLCEREQIPF
ILDRQEVANPMDFSDIENIARQYRYELFYRLCKERLFAGVFLGHHADDQAETILKRVFEGAHLGNLKGMARYGTYKGITL
LRPLLHITKRQIVEALDNYRIEYVQDATNADERFLRARMREQLFPYLQEIFGKNIRQPLLFLAEDSAELREYLDQQAAPF
LSQVIDNEIGQFLPVGQELLQTAFLTKWVCKQFFFKQGLVASKGFLQTVYDHLVRRSEARLRLRNRTVLVKARGVIIESI
Y

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=195, Percent_Identity=30.2564102564103, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_CHLMU (Q9PL79)

Other databases:

- EMBL:   AE002160
- PIR:   E81725
- RefSeq:   NP_296607.1
- ProteinModelPortal:   Q9PL79
- SMR:   Q9PL79
- GeneID:   1246397
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0228
- TIGR:   TC_0228
- HOGENOM:   HBG413885
- OMA:   FNLFRGT
- ProtClustDB:   CLSK871623
- BioCyc:   CMUR243161:TC_0228-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 37506; Mature: 37506

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIRLFENDKQLEVFFSSLDKKKKYLLALSGGSDSLLLMYLLRSRGISFTAVHVDYGWRE
CEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCC
TSYQEACDLASLCEREQIPFILDRQEVANPMDFSDIENIARQYRYELFYRLCKERLFAGV
CCHHHHHHHHHHHHHCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLGHHADDQAETILKRVFEGAHLGNLKGMARYGTYKGITLLRPLLHITKRQIVEALDNYR
HHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
IEYVQDATNADERFLRARMREQLFPYLQEIFGKNIRQPLLFLAEDSAELREYLDQQAAPF
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
LSQVIDNEIGQFLPVGQELLQTAFLTKWVCKQFFFKQGLVASKGFLQTVYDHLVRRSEAR
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LRLRNRTVLVKARGVIIESIY
HHHHCCEEEEEECCEEHHCCC
>Mature Secondary Structure
MVIRLFENDKQLEVFFSSLDKKKKYLLALSGGSDSLLLMYLLRSRGISFTAVHVDYGWRE
CEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCC
TSYQEACDLASLCEREQIPFILDRQEVANPMDFSDIENIARQYRYELFYRLCKERLFAGV
CCHHHHHHHHHHHHHCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLGHHADDQAETILKRVFEGAHLGNLKGMARYGTYKGITLLRPLLHITKRQIVEALDNYR
HHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
IEYVQDATNADERFLRARMREQLFPYLQEIFGKNIRQPLLFLAEDSAELREYLDQQAAPF
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
LSQVIDNEIGQFLPVGQELLQTAFLTKWVCKQFFFKQGLVASKGFLQTVYDHLVRRSEAR
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LRLRNRTVLVKARGVIIESIY
HHHHCCEEEEEECCEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935