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The map label for this gene is trmB

Identifier: 15834836

GI number: 15834836

Start: 257942

End: 258616

Strand: Direct

Name: trmB

Synonym: TC0216

Alternate gene names: 15834836

Gene position: 257942-258616 (Clockwise)

Preceding gene: 15834835

Following gene: 15834837

Centisome position: 24.04

GC content: 38.37

Gene sequence:

>675_bases
GTGAAACCACAAGATTTGAAATTACCCTATTTTTGGGAAGATCGTTGCCCAAAAATAGAAAATCACGTGTTCTATGTCCC
TAGTTACTATCCTAAATATGAAGAATTTGTGATGCCTTCTTGGCAAAATCTCTTTGCTAATGAAGGTCCTGTTTGTTGTG
AATTATGCTCTGGGAACGGAGATTGGGTAGTGGAACAGGCTTTAAAGGATACTTCGATCAATTGGATTGCGGTAGAAAAA
CGTTTTGATCGAGTTCGTAAGATTTGGTCAAAAATGAGCAATCATAAGGTAAATAATCTGCTCATTGTTTGTGGGGAAGC
ACAAACCTTTTTTACCCATTATGTGACGGATGCTTCCTTCCAAAAAATAGTCGTGAATTTCCCAGATCCTTGGCCTAAGT
TTCGACATCGCAAACATCGCCTATTTCAAGATGTTTTTGTCCAAGATATGGTGCGAGTTCTTGTAGAGGGTGGGCAGTTA
ACTTTGGTTACTGATGACCACACTTATCTAACGAACTCTATTCAAGTTATGCTTAATTATCTTTCTCCTGGGATGCAGGA
TCCTTATTACGTCAATGTGAAAGACAATTATGGTGGGTCTTGGTTTGAAAACTTATGGAGATCTAAGGGAGAGAAAATTT
TCTGTACAGAATTCGTAAAGAGAGTTGGGATATAG

Upstream 100 bases:

>100_bases
AAAATGGAAAAGGTCAAGCACTTCTCCTGAGACTGCTTTTAGAGTAGCAAGATTTGGGTATGGGGTTGGATGCAGCCTTT
CGTAGACAAAGTATTTTTCA

Downstream 100 bases:

>100_bases
CTGACTTGAACAGCTGACCTTCACGATGTCAACGTGACGCTCTAACCAACTGAGCTAATACCCCAAACAATTCGCATACT
TTATAAAGGAAATAGGATCT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKPQDLKLPYFWEDRCPKIENHVFYVPSYYPKYEEFVMPSWQNLFANEGPVCCELCSGNGDWVVEQALKDTSINWIAVEK
RFDRVRKIWSKMSNHKVNNLLIVCGEAQTFFTHYVTDASFQKIVVNFPDPWPKFRHRKHRLFQDVFVQDMVRVLVEGGQL
TLVTDDHTYLTNSIQVMLNYLSPGMQDPYYVNVKDNYGGSWFENLWRSKGEKIFCTEFVKRVGI

Sequences:

>Translated_224_residues
MKPQDLKLPYFWEDRCPKIENHVFYVPSYYPKYEEFVMPSWQNLFANEGPVCCELCSGNGDWVVEQALKDTSINWIAVEK
RFDRVRKIWSKMSNHKVNNLLIVCGEAQTFFTHYVTDASFQKIVVNFPDPWPKFRHRKHRLFQDVFVQDMVRVLVEGGQL
TLVTDDHTYLTNSIQVMLNYLSPGMQDPYYVNVKDNYGGSWFENLWRSKGEKIFCTEFVKRVGI
>Mature_224_residues
MKPQDLKLPYFWEDRCPKIENHVFYVPSYYPKYEEFVMPSWQNLFANEGPVCCELCSGNGDWVVEQALKDTSINWIAVEK
RFDRVRKIWSKMSNHKVNNLLIVCGEAQTFFTHYVTDASFQKIVVNFPDPWPKFRHRKHRLFQDVFVQDMVRVLVEGGQL
TLVTDDHTYLTNSIQVMLNYLSPGMQDPYYVNVKDNYGGSWFENLWRSKGEKIFCTEFVKRVGI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=140, Percent_Identity=27.8571428571429, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CHLMU (Q9PL91)

Other databases:

- EMBL:   AE002160
- PIR:   F81726
- RefSeq:   NP_296595.1
- ProteinModelPortal:   Q9PL91
- SMR:   Q9PL91
- GeneID:   1246342
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0216
- TIGR:   TC_0216
- HOGENOM:   HBG413880
- ProtClustDB:   PRK00121
- BioCyc:   CMUR243161:TC_0216-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26517; Mature: 26517

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 79-79 BINDING 106-106 BINDING 129-129 BINDING 133-133 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQDLKLPYFWEDRCPKIENHVFYVPSYYPKYEEFVMPSWQNLFANEGPVCCELCSGNG
CCCCCCCCCEEECCCCCCCCCCEEECCCCCCCHHHHCCCCHHHHHCCCCCEEEEEECCCC
DWVVEQALKDTSINWIAVEKRFDRVRKIWSKMSNHKVNNLLIVCGEAQTFFTHYVTDASF
CEEHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCC
QKIVVNFPDPWPKFRHRKHRLFQDVFVQDMVRVLVEGGQLTLVTDDHTYLTNSIQVMLNY
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHH
LSPGMQDPYYVNVKDNYGGSWFENLWRSKGEKIFCTEFVKRVGI
HCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEHHHHHHHHHCC
>Mature Secondary Structure
MKPQDLKLPYFWEDRCPKIENHVFYVPSYYPKYEEFVMPSWQNLFANEGPVCCELCSGNG
CCCCCCCCCEEECCCCCCCCCCEEECCCCCCCHHHHCCCCHHHHHCCCCCEEEEEECCCC
DWVVEQALKDTSINWIAVEKRFDRVRKIWSKMSNHKVNNLLIVCGEAQTFFTHYVTDASF
CEEHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCC
QKIVVNFPDPWPKFRHRKHRLFQDVFVQDMVRVLVEGGQLTLVTDDHTYLTNSIQVMLNY
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHH
LSPGMQDPYYVNVKDNYGGSWFENLWRSKGEKIFCTEFVKRVGI
HCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935