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The map label for this gene is dnaG

Identifier: 15834795

GI number: 15834795

Start: 207845

End: 209647

Strand: Direct

Name: dnaG

Synonym: TC0175

Alternate gene names: 15834795

Gene position: 207845-209647 (Clockwise)

Preceding gene: 15834792

Following gene: 15834796

Centisome position: 19.37

GC content: 40.99

Gene sequence:

>1803_bases
ATGTATTACACAGAAGAGAGTTTGGAGACTCTAAAGCATAGCATTGATATTGTCAGTGTTCTTGGAGAATACGTGCATTT
AAAGCGTAGCGGGGCAGATTACAAAGCTTGCTGTCCTTTCCATGATGAGAAGACTCCTTCGTTTATCGTATATCCAACAA
GAGGGCATTACCATTGTTATGGATGTGGGGAACATGGGGATGCGATCAACTTTCTTATGAAGCAGCAGGGATATTCGTTT
TCGGAAGCCGTGCTTTTCTTAGCTAAAAAATTTCATGTTGATCTTGTTATTAAAACAAAAACTAAAGAGTCTTCAGGTCA
AGATTCTAAAGAATGTTTACGTCGCATTAACAAAGAAGCGGAACGATTTTTTCAATATTGTTTATTGTGTTTGCCGGAGG
GAGGAGAGGCCTTGTCTTATTTATACAAGAGAGGGTTTTCTCTAGATACCATAGACCGTTTCCAGATAGGTTATGCTCCA
GAACAGAGAGTGTTTGTCCGAGCAATGGAGGAGCGGGGTATTTCTGTGAAACAGTTAGAGTGGGCAGGATATCTTTCTAA
AGATTGGTTTTTGTTCGCTCAGAGGATCATGTTTCCTATTCAAGATTCTTTAGGCTATACCATTGGCTTTTCTTCTCGAA
GATTTAAAGAAGGAGGAAAAGGAGGGAAGTACATTAACTCTCCAGAGACCCTTTTATTTAAGAAATCTCGAGTATTGTAT
GGGTTACAATTTTCTCGAAAACGAATAGCTAAAGAAAAAAGGGTGATTTTAGTTGAAGGTCAGGCCGACTGTTTGCAAAT
GATCGATTTTGGCTTCAATTGTACTTTGGCTGCTCAAGGGACTTCTTTCACCGAGACCCATGTAAAGGAGCTGGTTAAAT
TAGGGGTATCTAAAGCCTATTTATTGTTTGATGGGGATGCTGCAGGAGAGAAGGCTTCTTTACGTGTTGGCGATCTTTGT
CAGGTTGCAGGAATTGCAGTAATCGTTTGTCGTCTTCCTTCTGGGCAGGATCCTGATTCCTTTTTAATGCAAAGAGGGCC
AGAAGAGTTACGTGAGTTACTGGATAGAGGGGAAGATTATCTTTCTTTTCTTGTCTGGCACAAAATTCGCTCCTACGAGC
AGTTTACTCCTAGGGAAAAGGCCAGGGTAGTGGAGGAGGTTATTCAGCAGGTTCAGCATTGGGGAAGCCCCATTATGATT
CATGAATATCTGAGACAATTAGCTTCTTTGGTTAAAGTCCCCGAGGCCGCAGTTTTGAGTTATCTGTCTTCCATTAAGTC
TGCTACGGAGGACAAAGGCAAAAAGGCTGATGCGAAAGAAGTTTGCCCGGACCCTGAGGCTACTGCAGTAGCTTATAAAG
GGGGGAAAGCTTCAAAAAAAATTTCTCCTAGGATGATTTTAGAAGCTGATGTCATACGTTGTTTATTATTTGCTAAGCCT
GAAGAGGAGTTCGTCCCAGCCACAGTGAGACATTATCTTTCTCCTGAGGAGTTTCATTGTATAGAGTACCGGTCTATATT
TGTGATGGCAATGAATCATTACAACGAAAAGCATATGTTGCCTTCTATGGACGATATGATGGCGCTGGTTTGGGGAACGG
AAGCCATGACGCTTTTAGTAGATAGGAGGATAAATACTGAATTAATGCGGGACATTGTTGTTCAAGCAATCCAAAAATTA
TTAGACAAGCATTGGCGAGATCGAAAACGAAATTTCTGTCATCAGATGGGGAAAGAGTTAGATTCTTTACAAGAGTATGT
TCGTCTTTCTGAGGAGAGAATTAAGGTTTCTTTAGTTTCTTGA

Upstream 100 bases:

>100_bases
AAAGTGAAAAATAGTCTCACATTCCCAAGAAAATCCTAGTTCGTTACCATGCATCTCTCTTATGGCTAAGGGATAACAAG
AGGATTGGGACTAGGCTAGT

Downstream 100 bases:

>100_bases
TAAAGACTTTTAACCTGTTTTTAAAATGTTGATTGTTAGTTTGTTTTTATTATAAAATATTGGCCTGTTAACTATAAGTT
TATTAGGTTTTATGAGAAGT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MYYTEESLETLKHSIDIVSVLGEYVHLKRSGADYKACCPFHDEKTPSFIVYPTRGHYHCYGCGEHGDAINFLMKQQGYSF
SEAVLFLAKKFHVDLVIKTKTKESSGQDSKECLRRINKEAERFFQYCLLCLPEGGEALSYLYKRGFSLDTIDRFQIGYAP
EQRVFVRAMEERGISVKQLEWAGYLSKDWFLFAQRIMFPIQDSLGYTIGFSSRRFKEGGKGGKYINSPETLLFKKSRVLY
GLQFSRKRIAKEKRVILVEGQADCLQMIDFGFNCTLAAQGTSFTETHVKELVKLGVSKAYLLFDGDAAGEKASLRVGDLC
QVAGIAVIVCRLPSGQDPDSFLMQRGPEELRELLDRGEDYLSFLVWHKIRSYEQFTPREKARVVEEVIQQVQHWGSPIMI
HEYLRQLASLVKVPEAAVLSYLSSIKSATEDKGKKADAKEVCPDPEATAVAYKGGKASKKISPRMILEADVIRCLLFAKP
EEEFVPATVRHYLSPEEFHCIEYRSIFVMAMNHYNEKHMLPSMDDMMALVWGTEAMTLLVDRRINTELMRDIVVQAIQKL
LDKHWRDRKRNFCHQMGKELDSLQEYVRLSEERIKVSLVS

Sequences:

>Translated_600_residues
MYYTEESLETLKHSIDIVSVLGEYVHLKRSGADYKACCPFHDEKTPSFIVYPTRGHYHCYGCGEHGDAINFLMKQQGYSF
SEAVLFLAKKFHVDLVIKTKTKESSGQDSKECLRRINKEAERFFQYCLLCLPEGGEALSYLYKRGFSLDTIDRFQIGYAP
EQRVFVRAMEERGISVKQLEWAGYLSKDWFLFAQRIMFPIQDSLGYTIGFSSRRFKEGGKGGKYINSPETLLFKKSRVLY
GLQFSRKRIAKEKRVILVEGQADCLQMIDFGFNCTLAAQGTSFTETHVKELVKLGVSKAYLLFDGDAAGEKASLRVGDLC
QVAGIAVIVCRLPSGQDPDSFLMQRGPEELRELLDRGEDYLSFLVWHKIRSYEQFTPREKARVVEEVIQQVQHWGSPIMI
HEYLRQLASLVKVPEAAVLSYLSSIKSATEDKGKKADAKEVCPDPEATAVAYKGGKASKKISPRMILEADVIRCLLFAKP
EEEFVPATVRHYLSPEEFHCIEYRSIFVMAMNHYNEKHMLPSMDDMMALVWGTEAMTLLVDRRINTELMRDIVVQAIQKL
LDKHWRDRKRNFCHQMGKELDSLQEYVRLSEERIKVSLVS
>Mature_600_residues
MYYTEESLETLKHSIDIVSVLGEYVHLKRSGADYKACCPFHDEKTPSFIVYPTRGHYHCYGCGEHGDAINFLMKQQGYSF
SEAVLFLAKKFHVDLVIKTKTKESSGQDSKECLRRINKEAERFFQYCLLCLPEGGEALSYLYKRGFSLDTIDRFQIGYAP
EQRVFVRAMEERGISVKQLEWAGYLSKDWFLFAQRIMFPIQDSLGYTIGFSSRRFKEGGKGGKYINSPETLLFKKSRVLY
GLQFSRKRIAKEKRVILVEGQADCLQMIDFGFNCTLAAQGTSFTETHVKELVKLGVSKAYLLFDGDAAGEKASLRVGDLC
QVAGIAVIVCRLPSGQDPDSFLMQRGPEELRELLDRGEDYLSFLVWHKIRSYEQFTPREKARVVEEVIQQVQHWGSPIMI
HEYLRQLASLVKVPEAAVLSYLSSIKSATEDKGKKADAKEVCPDPEATAVAYKGGKASKKISPRMILEADVIRCLLFAKP
EEEFVPATVRHYLSPEEFHCIEYRSIFVMAMNHYNEKHMLPSMDDMMALVWGTEAMTLLVDRRINTELMRDIVVQAIQKL
LDKHWRDRKRNFCHQMGKELDSLQEYVRLSEERIKVSLVS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=408, Percent_Identity=33.8235294117647, Blast_Score=201, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_CHLMU (Q9PLC9)

Other databases:

- EMBL:   AE002160
- PIR:   H81733
- RefSeq:   NP_296554.1
- ProteinModelPortal:   Q9PLC9
- SMR:   Q9PLC9
- GeneID:   1246300
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0175
- TIGR:   TC_0175
- HOGENOM:   HBG413863
- OMA:   QAIQYID
- ProtClustDB:   PRK05667
- BioCyc:   CMUR243161:TC_0175-MONOMER
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 68811; Mature: 68811

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYTEESLETLKHSIDIVSVLGEYVHLKRSGADYKACCPFHDEKTPSFIVYPTRGHYHCY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEEECCCCEEEE
GCGEHGDAINFLMKQQGYSFSEAVLFLAKKFHVDLVIKTKTKESSGQDSKECLRRINKEA
ECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHH
ERFFQYCLLCLPEGGEALSYLYKRGFSLDTIDRFQIGYAPEQRVFVRAMEERGISVKQLE
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHEECCCCCCHHHHHHHHHHCCCCEEEEH
WAGYLSKDWFLFAQRIMFPIQDSLGYTIGFSSRRFKEGGKGGKYINSPETLLFKKSRVLY
HCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCCHHHEEECCHHHC
GLQFSRKRIAKEKRVILVEGQADCLQMIDFGFNCTLAAQGTSFTETHVKELVKLGVSKAY
CHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEE
LLFDGDAAGEKASLRVGDLCQVAGIAVIVCRLPSGQDPDSFLMQRGPEELRELLDRGEDY
EEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHCCHHHHHHHHHCCHHH
LSFLVWHKIRSYEQFTPREKARVVEEVIQQVQHWGSPIMIHEYLRQLASLVKVPEAAVLS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHH
YLSSIKSATEDKGKKADAKEVCPDPEATAVAYKGGKASKKISPRMILEADVIRCLLFAKP
HHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
EEEFVPATVRHYLSPEEFHCIEYRSIFVMAMNHYNEKHMLPSMDDMMALVWGTEAMTLLV
CHHHCCHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHH
DRRINTELMRDIVVQAIQKLLDKHWRDRKRNFCHQMGKELDSLQEYVRLSEERIKVSLVS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MYYTEESLETLKHSIDIVSVLGEYVHLKRSGADYKACCPFHDEKTPSFIVYPTRGHYHCY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEEECCCCEEEE
GCGEHGDAINFLMKQQGYSFSEAVLFLAKKFHVDLVIKTKTKESSGQDSKECLRRINKEA
ECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHH
ERFFQYCLLCLPEGGEALSYLYKRGFSLDTIDRFQIGYAPEQRVFVRAMEERGISVKQLE
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHEECCCCCCHHHHHHHHHHCCCCEEEEH
WAGYLSKDWFLFAQRIMFPIQDSLGYTIGFSSRRFKEGGKGGKYINSPETLLFKKSRVLY
HCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCCHHHEEECCHHHC
GLQFSRKRIAKEKRVILVEGQADCLQMIDFGFNCTLAAQGTSFTETHVKELVKLGVSKAY
CHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEE
LLFDGDAAGEKASLRVGDLCQVAGIAVIVCRLPSGQDPDSFLMQRGPEELRELLDRGEDY
EEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHCCHHHHHHHHHCCHHH
LSFLVWHKIRSYEQFTPREKARVVEEVIQQVQHWGSPIMIHEYLRQLASLVKVPEAAVLS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHH
YLSSIKSATEDKGKKADAKEVCPDPEATAVAYKGGKASKKISPRMILEADVIRCLLFAKP
HHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
EEEFVPATVRHYLSPEEFHCIEYRSIFVMAMNHYNEKHMLPSMDDMMALVWGTEAMTLLV
CHHHCCHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHH
DRRINTELMRDIVVQAIQKLLDKHWRDRKRNFCHQMGKELDSLQEYVRLSEERIKVSLVS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935