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The map label for this gene is lysS

Identifier: 15834783

GI number: 15834783

Start: 195739

End: 197313

Strand: Direct

Name: lysS

Synonym: TC0163

Alternate gene names: 15834783

Gene position: 195739-197313 (Clockwise)

Preceding gene: 15834782

Following gene: 15834786

Centisome position: 18.24

GC content: 40.06

Gene sequence:

>1575_bases
ATGTCTGCAGAAGTTGAATATTTGCAACACGAAGATTATTTGTATAGAACCATTAAGTTAAAAGAAATCAGAGAATTAGG
AATAAATCCATACCCCTACCAGTACGCTGATTGTGTTGAGGTACAGGAAATTCGTAATCGGGTTGTAGATAATGAATTGG
GGGATAGCGAAGCTGCTTTTCGGAAAGAGACTCCTAAAGTGCGGTTTGCTGGAAGGCTGGTTCTTTTCCGTTCTATGGGT
AAAAATGCTTTTGGGCAAATCCTTGATAGGGATGCTAAAATTCAAGTTATGTTTAATCGAGATTTTTCTCAGGTAGCAGG
GTTACCTGCTGATTCTGAAATTTCTTCCATTAAATTTATTGAGAAGAAGCTGGACTTAGGGGATATCTTAGGTATAGATG
GATATCTTTTCTTCACACATTCAGGAGAACTAACAGTCCTTGTTGAGACTGTAACGTTGTTATGTAAATCTTTAATTTCT
CTGCCTGATAAGCATTCAGGATTAGCTGATAAGGAAATTCGTTATCGTAAGCGCTGGGCTGATCTTATTTCATCAGAGGA
AGTTCGTAAAACATTTTTAGCAAGAAGTCGGATTCTTAAGTTGATTCGTGAATACATGGATCAACAGGATTTTTTAGAAG
TGGAAACCCCTGTACTGCAAACCATCTATGGGGGAGCAGAGGCAACTCCTTTTGTTACCACATTAAAAGCTCTGCATACA
GAAATGTTCCTTAGAATTTCTTTAGAGATCGCATTGAAGAAAATCCTTGTTGGGGGGATGTCCCGTGTTTATGAAATCGG
GAAAGTGTTCCGTAATGAGGGGATCGATAGGACGCATAATCCAGAGTTCACGATGATCGAGGCATATGCTGCTTATTGGG
ATTACAATGATGTGATGAAATGTGTGGAAAACCTTGTTGAATATGTCGTACGAGCATTGAATAATGGAGAGACAAAAGTA
CAGTATTCTCATTTGAAATCCGGCCCTCAGATTGTCGATTTCAAAGCTCCTTGGATTCGTATGACCATGAAAGAAAGTAT
TTCTGTTTACGGAGGTGTTGATGTTGATCAATACTCAGAGCATGAGTTGCGAGACATTCTAAAAACACGAACAGCTCTTC
CTGAGAAAGCTTATGTTTCTGCTACTCGAGGGGAAATGATTGCTTTGCTATTTGATGAGCTGGTGTGTGATAAGCTTATT
GCGCCACATCACATTACGGATCATCCATTAGAAACAACTCCTCTTTGCAAAACATTGCGTTCTGGGGATGAAACTTTGGT
GGAAAGATTTGAAAGCTTCTGTTTAGGGAAGGAGCTATGCAATGCTTACTCAGAGCTCAACGATCCTTTGCAACAGCGAA
AACTATTAGAAGAGCAAATGCGGAAAAAGGCTTTGAATCCTGATAGCGAATACCATCCTATAGACGAAGAATTTTTAGAA
GCTCTCTGCCAAGGAATGCCACCAGCAGGGGGATTCGGAATAGGAATAGATCGGTTAGTTATGATGTTGACAGATGCCGC
ATCCATTCGAGACGTTCTGTACTTTCCTGTTATGCGGCGTATAGAGTCAAAATAG

Upstream 100 bases:

>100_bases
AATTTCTCAAGTTTGATGTAGCATAGGTCTGCTTAGGACCTTCGGAATTGTCATAAGAAGGAAAATATTGCGACCATTTT
TTTGAGCCAAAGGACGGTTA

Downstream 100 bases:

>100_bases
GACTAAATCTTTTTCACTTTCATCCCGGTTTTGCCGTCTTCGATGGCAAAACCTCTGGATATGATCTCATCTCGAAGGCG
ATCTGCTTCTTTCCAATTCT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSAEVEYLQHEDYLYRTIKLKEIRELGINPYPYQYADCVEVQEIRNRVVDNELGDSEAAFRKETPKVRFAGRLVLFRSMG
KNAFGQILDRDAKIQVMFNRDFSQVAGLPADSEISSIKFIEKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLIS
LPDKHSGLADKEIRYRKRWADLISSEEVRKTFLARSRILKLIREYMDQQDFLEVETPVLQTIYGGAEATPFVTTLKALHT
EMFLRISLEIALKKILVGGMSRVYEIGKVFRNEGIDRTHNPEFTMIEAYAAYWDYNDVMKCVENLVEYVVRALNNGETKV
QYSHLKSGPQIVDFKAPWIRMTMKESISVYGGVDVDQYSEHELRDILKTRTALPEKAYVSATRGEMIALLFDELVCDKLI
APHHITDHPLETTPLCKTLRSGDETLVERFESFCLGKELCNAYSELNDPLQQRKLLEEQMRKKALNPDSEYHPIDEEFLE
ALCQGMPPAGGFGIGIDRLVMMLTDAASIRDVLYFPVMRRIESK

Sequences:

>Translated_524_residues
MSAEVEYLQHEDYLYRTIKLKEIRELGINPYPYQYADCVEVQEIRNRVVDNELGDSEAAFRKETPKVRFAGRLVLFRSMG
KNAFGQILDRDAKIQVMFNRDFSQVAGLPADSEISSIKFIEKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLIS
LPDKHSGLADKEIRYRKRWADLISSEEVRKTFLARSRILKLIREYMDQQDFLEVETPVLQTIYGGAEATPFVTTLKALHT
EMFLRISLEIALKKILVGGMSRVYEIGKVFRNEGIDRTHNPEFTMIEAYAAYWDYNDVMKCVENLVEYVVRALNNGETKV
QYSHLKSGPQIVDFKAPWIRMTMKESISVYGGVDVDQYSEHELRDILKTRTALPEKAYVSATRGEMIALLFDELVCDKLI
APHHITDHPLETTPLCKTLRSGDETLVERFESFCLGKELCNAYSELNDPLQQRKLLEEQMRKKALNPDSEYHPIDEEFLE
ALCQGMPPAGGFGIGIDRLVMMLTDAASIRDVLYFPVMRRIESK
>Mature_523_residues
SAEVEYLQHEDYLYRTIKLKEIRELGINPYPYQYADCVEVQEIRNRVVDNELGDSEAAFRKETPKVRFAGRLVLFRSMGK
NAFGQILDRDAKIQVMFNRDFSQVAGLPADSEISSIKFIEKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLISL
PDKHSGLADKEIRYRKRWADLISSEEVRKTFLARSRILKLIREYMDQQDFLEVETPVLQTIYGGAEATPFVTTLKALHTE
MFLRISLEIALKKILVGGMSRVYEIGKVFRNEGIDRTHNPEFTMIEAYAAYWDYNDVMKCVENLVEYVVRALNNGETKVQ
YSHLKSGPQIVDFKAPWIRMTMKESISVYGGVDVDQYSEHELRDILKTRTALPEKAYVSATRGEMIALLFDELVCDKLIA
PHHITDHPLETTPLCKTLRSGDETLVERFESFCLGKELCNAYSELNDPLQQRKLLEEQMRKKALNPDSEYHPIDEEFLEA
LCQGMPPAGGFGIGIDRLVMMLTDAASIRDVLYFPVMRRIESK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=518, Percent_Identity=39.96138996139, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI194272210, Length=518, Percent_Identity=39.96138996139, Blast_Score=326, Evalue=4e-89,
Organism=Escherichia coli, GI1790571, Length=460, Percent_Identity=41.304347826087, Blast_Score=335, Evalue=5e-93,
Organism=Escherichia coli, GI1789256, Length=463, Percent_Identity=40.8207343412527, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI87082379, Length=336, Percent_Identity=29.1666666666667, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788173, Length=308, Percent_Identity=24.6753246753247, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=522, Percent_Identity=36.7816091954023, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17535925, Length=522, Percent_Identity=36.7816091954023, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI71994340, Length=506, Percent_Identity=37.5494071146245, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI32566633, Length=219, Percent_Identity=26.027397260274, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=520, Percent_Identity=37.8846153846154, Blast_Score=307, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6324256, Length=487, Percent_Identity=32.0328542094456, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=336, Percent_Identity=25.297619047619, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=490, Percent_Identity=22.0408163265306, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640849, Length=529, Percent_Identity=38.5633270321361, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24640851, Length=529, Percent_Identity=38.5633270321361, Blast_Score=322, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 60082; Mature: 59951

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEVEYLQHEDYLYRTIKLKEIRELGINPYPYQYADCVEVQEIRNRVVDNELGDSEAAF
CCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
RKETPKVRFAGRLVLFRSMGKNAFGQILDRDAKIQVMFNRDFSQVAGLPADSEISSIKFI
HCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCHHHHHHH
EKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLISLPDKHSGLADKEIRYRKRWA
HHHCCHHHEECCCCEEEEEECCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
DLISSEEVRKTFLARSRILKLIREYMDQQDFLEVETPVLQTIYGGAEATPFVTTLKALHT
HHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EMFLRISLEIALKKILVGGMSRVYEIGKVFRNEGIDRTHNPEFTMIEAYAAYWDYNDVMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHCCHHHHHH
CVENLVEYVVRALNNGETKVQYSHLKSGPQIVDFKAPWIRMTMKESISVYGGVDVDQYSE
HHHHHHHHHHHHHCCCCCEEEHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCH
HELRDILKTRTALPEKAYVSATRGEMIALLFDELVCDKLIAPHHITDHPLETTPLCKTLR
HHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
SGDETLVERFESFCLGKELCNAYSELNDPLQQRKLLEEQMRKKALNPDSEYHPIDEEFLE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ALCQGMPPAGGFGIGIDRLVMMLTDAASIRDVLYFPVMRRIESK
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAEVEYLQHEDYLYRTIKLKEIRELGINPYPYQYADCVEVQEIRNRVVDNELGDSEAAF
CCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
RKETPKVRFAGRLVLFRSMGKNAFGQILDRDAKIQVMFNRDFSQVAGLPADSEISSIKFI
HCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCHHHHHHH
EKKLDLGDILGIDGYLFFTHSGELTVLVETVTLLCKSLISLPDKHSGLADKEIRYRKRWA
HHHCCHHHEECCCCEEEEEECCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
DLISSEEVRKTFLARSRILKLIREYMDQQDFLEVETPVLQTIYGGAEATPFVTTLKALHT
HHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EMFLRISLEIALKKILVGGMSRVYEIGKVFRNEGIDRTHNPEFTMIEAYAAYWDYNDVMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHCCHHHHHH
CVENLVEYVVRALNNGETKVQYSHLKSGPQIVDFKAPWIRMTMKESISVYGGVDVDQYSE
HHHHHHHHHHHHHCCCCCEEEHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCH
HELRDILKTRTALPEKAYVSATRGEMIALLFDELVCDKLIAPHHITDHPLETTPLCKTLR
HHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
SGDETLVERFESFCLGKELCNAYSELNDPLQQRKLLEEQMRKKALNPDSEYHPIDEEFLE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ALCQGMPPAGGFGIGIDRLVMMLTDAASIRDVLYFPVMRRIESK
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA