Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 15834767

GI number: 15834767

Start: 178893

End: 179837

Strand: Direct

Name: miaA

Synonym: TC0147

Alternate gene names: 15834767

Gene position: 178893-179837 (Clockwise)

Preceding gene: 15834766

Following gene: 15834769

Centisome position: 16.67

GC content: 42.12

Gene sequence:

>945_bases
ATGTTTAAGCGGACGGTGATTTTATTAGCTGGCCCAACAGGATCAGGGAAAACTGCTGTTTCTCTTAAGTTGGCTCCGCT
TGTTGATGGAGAGATTATCTCTGTAGACTCTATGCAAGTCTATCAGGGAATGGATATTGGAACAGCAAAGGTCTCCTTAG
CTATTCGTCAGGCTGTTCCACATCATCTGATAGATATTTGTCATGTCCAAGAGACTTTTAATGCTGTGGATTTCTATTAT
CACGCGATACAAGCCTGTCAAGATATTCTATCTCGTAATAAAGTTCCGATTCTTGTAGGGGGAACAGGGTTTTATTTTCA
TACTTTTTTGTCTGGTCCTCCATCAGGGCCTTCTCCTGACTTTGCATTGCGAGAGCAACTTTCATTGGAAGCTCAAGAAC
GTGGTATAGGAGCTCTCTATCAAGAGTTAGTGGAGTTGGATTCGGCCTATGCTGCTACTATTACTAAGCATGATAAGAAT
AAAATTATTCGTGCTTTGGAAATTATTCGGAAGACGGGGAATAAGGTTTCTAGTTATTCCTGGGAATCTACAGTAAACGA
GTCGAAAGAGTATCATTGTCGAGGTTGGCTTTTGTCTCCAGACCCTGAATTATTGCGCCATAATATTTTGGAACGGTGCG
ATCAGATGTTAGAAGAAGGCCTCGTAGATGAGGTTCGCTCTCTTGTAGCTTTAGGGATCAAAGGGAATACATCAGCTTCT
CGGGCAATTGGCTATAGAGAATGGATAGAATTTTTAGATGCGGGGAGCCCCGTAGAACGATTTGAGGATACCAAGCAAAA
GTTTATCACTAACACCTGGCGCTATACGAAAAAACAACGTACTTGGTTTAAACGTTATCCACTATTTAGAGAACTGAGCC
CTATGGGCATGACTCTTGGTGATATCGCAAGAAAGATAGCGCAGGACTACTTTCTATGTGGCTAG

Upstream 100 bases:

>100_bases
AGAATCTTTTAGTGATTTTTAAACTATGCCCTTACCAGCTTCTTCGGGATCTGTTGCAGGATTCTCTGTGTGTTCAGATC
CTCAAAAATCTTTTTCAAAA

Downstream 100 bases:

>100_bases
ATCTTCGTTGAGAAGGCAGGAGAAACTGGGAGATAGAAGGTATCTCGTTCCACAGGAGTAAATCCTGCGCGGCTAAGCAT
CATACGAATTTCTTGCTCAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MFKRTVILLAGPTGSGKTAVSLKLAPLVDGEIISVDSMQVYQGMDIGTAKVSLAIRQAVPHHLIDICHVQETFNAVDFYY
HAIQACQDILSRNKVPILVGGTGFYFHTFLSGPPSGPSPDFALREQLSLEAQERGIGALYQELVELDSAYAATITKHDKN
KIIRALEIIRKTGNKVSSYSWESTVNESKEYHCRGWLLSPDPELLRHNILERCDQMLEEGLVDEVRSLVALGIKGNTSAS
RAIGYREWIEFLDAGSPVERFEDTKQKFITNTWRYTKKQRTWFKRYPLFRELSPMGMTLGDIARKIAQDYFLCG

Sequences:

>Translated_314_residues
MFKRTVILLAGPTGSGKTAVSLKLAPLVDGEIISVDSMQVYQGMDIGTAKVSLAIRQAVPHHLIDICHVQETFNAVDFYY
HAIQACQDILSRNKVPILVGGTGFYFHTFLSGPPSGPSPDFALREQLSLEAQERGIGALYQELVELDSAYAATITKHDKN
KIIRALEIIRKTGNKVSSYSWESTVNESKEYHCRGWLLSPDPELLRHNILERCDQMLEEGLVDEVRSLVALGIKGNTSAS
RAIGYREWIEFLDAGSPVERFEDTKQKFITNTWRYTKKQRTWFKRYPLFRELSPMGMTLGDIARKIAQDYFLCG
>Mature_314_residues
MFKRTVILLAGPTGSGKTAVSLKLAPLVDGEIISVDSMQVYQGMDIGTAKVSLAIRQAVPHHLIDICHVQETFNAVDFYY
HAIQACQDILSRNKVPILVGGTGFYFHTFLSGPPSGPSPDFALREQLSLEAQERGIGALYQELVELDSAYAATITKHDKN
KIIRALEIIRKTGNKVSSYSWESTVNESKEYHCRGWLLSPDPELLRHNILERCDQMLEEGLVDEVRSLVALGIKGNTSAS
RAIGYREWIEFLDAGSPVERFEDTKQKFITNTWRYTKKQRTWFKRYPLFRELSPMGMTLGDIARKIAQDYFLCG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=333, Percent_Identity=29.4294294294294, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1790613, Length=281, Percent_Identity=34.1637010676157, Blast_Score=160, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI25144712, Length=238, Percent_Identity=27.7310924369748, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=31.7567567567568, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24649830, Length=271, Percent_Identity=31.3653136531365, Blast_Score=118, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHLMU (Q9PLF7)

Other databases:

- EMBL:   AE002160
- PIR:   C81735
- RefSeq:   NP_296526.1
- ProteinModelPortal:   Q9PLF7
- SMR:   Q9PLF7
- GeneID:   1245681
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0147
- TIGR:   TC_0147
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q9PLF7
- ProtClustDB:   PRK00091
- BioCyc:   CMUR243161:TC_0147-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35396; Mature: 35396

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRTVILLAGPTGSGKTAVSLKLAPLVDGEIISVDSMQVYQGMDIGTAKVSLAIRQAVP
CCCEEEEEEECCCCCCCEEEEEEEEECCCCCEEEECCHHHHCCCCCCHHHHHHHHHHHHH
HHLIDICHVQETFNAVDFYYHAIQACQDILSRNKVPILVGGTGFYFHTFLSGPPSGPSPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCC
FALREQLSLEAQERGIGALYQELVELDSAYAATITKHDKNKIIRALEIIRKTGNKVSSYS
HHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
WESTVNESKEYHCRGWLLSPDPELLRHNILERCDQMLEEGLVDEVRSLVALGIKGNTSAS
CHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
RAIGYREWIEFLDAGSPVERFEDTKQKFITNTWRYTKKQRTWFKRYPLFRELSPMGMTLG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHH
DIARKIAQDYFLCG
HHHHHHHHHHCCCC
>Mature Secondary Structure
MFKRTVILLAGPTGSGKTAVSLKLAPLVDGEIISVDSMQVYQGMDIGTAKVSLAIRQAVP
CCCEEEEEEECCCCCCCEEEEEEEEECCCCCEEEECCHHHHCCCCCCHHHHHHHHHHHHH
HHLIDICHVQETFNAVDFYYHAIQACQDILSRNKVPILVGGTGFYFHTFLSGPPSGPSPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCC
FALREQLSLEAQERGIGALYQELVELDSAYAATITKHDKNKIIRALEIIRKTGNKVSSYS
HHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
WESTVNESKEYHCRGWLLSPDPELLRHNILERCDQMLEEGLVDEVRSLVALGIKGNTSAS
CHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
RAIGYREWIEFLDAGSPVERFEDTKQKFITNTWRYTKKQRTWFKRYPLFRELSPMGMTLG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHH
DIARKIAQDYFLCG
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935