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The map label for this gene is murD

Identifier: 15834759

GI number: 15834759

Start: 170341

End: 171642

Strand: Direct

Name: murD

Synonym: TC0139

Alternate gene names: 15834759

Gene position: 170341-171642 (Clockwise)

Preceding gene: 15834758

Following gene: 15834760

Centisome position: 15.88

GC content: 41.71

Gene sequence:

>1302_bases
TTGTGTTGCCATTGGTATTTTCGCTGTATTTTGGGGTTAGAGGATAAGCGAATGGGATTAAAACGTGTTGTAGTTATTGG
CTTAGGACTATCTGGTAAGTCTATTGCGCGTTTCTTAGCTCGAAAAGGCGTGTATGTGTTGGGAGTAGATAGTTCTATTC
AGGCGTTGCAACATTGTCCGTATATTCATGAAAAATTGTTAGAAACAGATGAGTTTCCTACACAGGTAGATTATGTTGTA
CGTTCTCCGGGGATTTCTAAGGATCATCCTTGGGTCAAGGCTGCAAGAGCTGCTCAAATCTCTGTAGTGACAGATATTCA
ACTAGCTTTTCAGACTAAGGAGTTTATAGAACAGAAGTCTTTTGGAATTACAGGGACTGTAGGGAAAACGACCACAATAT
TATTTTTAGAATATTTGCTAAGAAAAGCGGGAATCCCAGCTTTTGCCATGGGGAATGTGGGAGTCCCTATTTTGGATGGT
ATGCAGAATTCGGGAGTCCGGCTTGTTGAAATGAGTTCTTTCCAGCTCGCTGATCAAGAAACCTCTTATCCGGTCTTATC
AGGGGGAATGATTCTTAATATCTCGGATAATCATTTGGATTATCACGGGAGTTTTTTAGAGTATTGTCAATCCAAACAGA
ATCTCTCTTTATGTATGAGGAATCCAGAAGATCTTTGGGTTGGGGATCAGCGTTTTTGCGGGCGTAGTTACTGGGAAGAA
GTGCAAAAGTACATGCGTTTACTAGATAAAGAAAGTGCATTAAAACCATTGTACTTGCATGATAAGTACAACTACTGTTG
TGCATATCTGCTTGCTCAAGCAGAATTTCCGATAGCTAAATCCCTTTTTATAGAGGCCGTAGCTACTTTTAAGAAACCTT
CGCATAGGATGGAATATTTAGGGGAAAAGTGTGGTGTGCATTATATCAATGATAGTAAGGCAACTACAGTACGCGCTACG
GAAAAGGCTCTTTTGAGCATAGGAAGTCGGGCAATTGTGATTCTTGGGGGTAGAAATAAAGGATACTCGTTCGTTTCCTT
GCTGCCTTCTTTGCGAAGGTTTGCGAAGAGTGTGGTTGCTATGGGAGAGTGTGCTCAGGAGATAGCTCAGGATTTGGACG
GCTTCCCAGTAACAGTAGTGAGAAATTTACATGAAGCTTTGCTATGCGCTGAAGAGCAAGCGATTCCCGGAGATGTTGTT
TTATTGTCTCCAGCTTGCGCTAGCTTTGATCAATTTCGTAGCTATGAAGAGCGGGGAGCGATATTTAAGCAGTTGGTTGG
GATGGAAGAGGTTCTTTTATGA

Upstream 100 bases:

>100_bases
AAAAAAGAGTTTTTCTTTGCTCTCCTCTACATCACCATTATGAATATAAAGGGTATCCAGAAAAGGTTGTGGTGAGAAAT
TTTTGGATTATTGAGTTCCT

Downstream 100 bases:

>100_bases
ATCGTAGAAACACGATGATTGTAGCAGCTTCTGTGAATGCAGTACTCTTGGCAGTGCTGTTTATGACAGCACGCTATTCA
GAGCAAGAGGTAGAGTATTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MCCHWYFRCILGLEDKRMGLKRVVVIGLGLSGKSIARFLARKGVYVLGVDSSIQALQHCPYIHEKLLETDEFPTQVDYVV
RSPGISKDHPWVKAARAAQISVVTDIQLAFQTKEFIEQKSFGITGTVGKTTTILFLEYLLRKAGIPAFAMGNVGVPILDG
MQNSGVRLVEMSSFQLADQETSYPVLSGGMILNISDNHLDYHGSFLEYCQSKQNLSLCMRNPEDLWVGDQRFCGRSYWEE
VQKYMRLLDKESALKPLYLHDKYNYCCAYLLAQAEFPIAKSLFIEAVATFKKPSHRMEYLGEKCGVHYINDSKATTVRAT
EKALLSIGSRAIVILGGRNKGYSFVSLLPSLRRFAKSVVAMGECAQEIAQDLDGFPVTVVRNLHEALLCAEEQAIPGDVV
LLSPACASFDQFRSYEERGAIFKQLVGMEEVLL

Sequences:

>Translated_433_residues
MCCHWYFRCILGLEDKRMGLKRVVVIGLGLSGKSIARFLARKGVYVLGVDSSIQALQHCPYIHEKLLETDEFPTQVDYVV
RSPGISKDHPWVKAARAAQISVVTDIQLAFQTKEFIEQKSFGITGTVGKTTTILFLEYLLRKAGIPAFAMGNVGVPILDG
MQNSGVRLVEMSSFQLADQETSYPVLSGGMILNISDNHLDYHGSFLEYCQSKQNLSLCMRNPEDLWVGDQRFCGRSYWEE
VQKYMRLLDKESALKPLYLHDKYNYCCAYLLAQAEFPIAKSLFIEAVATFKKPSHRMEYLGEKCGVHYINDSKATTVRAT
EKALLSIGSRAIVILGGRNKGYSFVSLLPSLRRFAKSVVAMGECAQEIAQDLDGFPVTVVRNLHEALLCAEEQAIPGDVV
LLSPACASFDQFRSYEERGAIFKQLVGMEEVLL
>Mature_433_residues
MCCHWYFRCILGLEDKRMGLKRVVVIGLGLSGKSIARFLARKGVYVLGVDSSIQALQHCPYIHEKLLETDEFPTQVDYVV
RSPGISKDHPWVKAARAAQISVVTDIQLAFQTKEFIEQKSFGITGTVGKTTTILFLEYLLRKAGIPAFAMGNVGVPILDG
MQNSGVRLVEMSSFQLADQETSYPVLSGGMILNISDNHLDYHGSFLEYCQSKQNLSLCMRNPEDLWVGDQRFCGRSYWEE
VQKYMRLLDKESALKPLYLHDKYNYCCAYLLAQAEFPIAKSLFIEAVATFKKPSHRMEYLGEKCGVHYINDSKATTVRAT
EKALLSIGSRAIVILGGRNKGYSFVSLLPSLRRFAKSVVAMGECAQEIAQDLDGFPVTVVRNLHEALLCAEEQAIPGDVV
LLSPACASFDQFRSYEERGAIFKQLVGMEEVLL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=442, Percent_Identity=30.5429864253394, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48421; Mature: 48421

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCCHWYFRCILGLEDKRMGLKRVVVIGLGLSGKSIARFLARKGVYVLGVDSSIQALQHCP
CCHHHHHHHHHCCCHHHCCCEEEEEEEECCCHHHHHHHHHHCCEEEEECCHHHHHHHHCC
YIHEKLLETDEFPTQVDYVVRSPGISKDHPWVKAARAAQISVVTDIQLAFQTKEFIEQKS
HHHHHHHCCCCCCCHHHHHEECCCCCCCCCHHHHHHHHEEEEEEHHHHHHHHHHHHHHHC
FGITGTVGKTTTILFLEYLLRKAGIPAFAMGNVGVPILDGMQNSGVRLVEMSSFQLADQE
CCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCCCCCCEEEEEECCCEECCCC
TSYPVLSGGMILNISDNHLDYHGSFLEYCQSKQNLSLCMRNPEDLWVGDQRFCGRSYWEE
CCCCEEECCEEEEEECCCCCCCCHHHHHHCCCCCCCEEECCCHHCCCCCHHHHCHHHHHH
VQKYMRLLDKESALKPLYLHDKYNYCCAYLLAQAEFPIAKSLFIEAVATFKKPSHRMEYL
HHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHH
GEKCGVHYINDSKATTVRATEKALLSIGSRAIVILGGRNKGYSFVSLLPSLRRFAKSVVA
HHHCCEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
MGECAQEIAQDLDGFPVTVVRNLHEALLCAEEQAIPGDVVLLSPACASFDQFRSYEERGA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHH
IFKQLVGMEEVLL
HHHHHHHHHHHCC
>Mature Secondary Structure
MCCHWYFRCILGLEDKRMGLKRVVVIGLGLSGKSIARFLARKGVYVLGVDSSIQALQHCP
CCHHHHHHHHHCCCHHHCCCEEEEEEEECCCHHHHHHHHHHCCEEEEECCHHHHHHHHCC
YIHEKLLETDEFPTQVDYVVRSPGISKDHPWVKAARAAQISVVTDIQLAFQTKEFIEQKS
HHHHHHHCCCCCCCHHHHHEECCCCCCCCCHHHHHHHHEEEEEEHHHHHHHHHHHHHHHC
FGITGTVGKTTTILFLEYLLRKAGIPAFAMGNVGVPILDGMQNSGVRLVEMSSFQLADQE
CCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCCCCCCEEEEEECCCEECCCC
TSYPVLSGGMILNISDNHLDYHGSFLEYCQSKQNLSLCMRNPEDLWVGDQRFCGRSYWEE
CCCCEEECCEEEEEECCCCCCCCHHHHHHCCCCCCCEEECCCHHCCCCCHHHHCHHHHHH
VQKYMRLLDKESALKPLYLHDKYNYCCAYLLAQAEFPIAKSLFIEAVATFKKPSHRMEYL
HHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHH
GEKCGVHYINDSKATTVRATEKALLSIGSRAIVILGGRNKGYSFVSLLPSLRRFAKSVVA
HHHCCEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
MGECAQEIAQDLDGFPVTVVRNLHEALLCAEEQAIPGDVVLLSPACASFDQFRSYEERGA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHH
IFKQLVGMEEVLL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]