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The map label for this gene is ribH

Identifier: 15834730

GI number: 15834730

Start: 124310

End: 124783

Strand: Direct

Name: ribH

Synonym: TC0105

Alternate gene names: 15834730

Gene position: 124310-124783 (Clockwise)

Preceding gene: 15834729

Following gene: 15834732

Centisome position: 11.59

GC content: 43.88

Gene sequence:

>474_bases
ATGAAACTGTTGAAAGGACTTCCTGTTGCTAAGGATGTGCGTGTAGCTATAGTCGGGGCGTGCTTTAACGCTCCTATTGC
TGATCGCTTGGTTTCCGGAGCTCGAGAGACATTTTTTGAGTCCGGAGGTAGTCCCGATTCTTTGACTGTTGTTCGAGTTC
CCGGTGCTTTTGAAATTCCCTGTGCCATTAAAAAAATGCTCTCTAAAGGAAATCTTTTTCAAGCAATTGTTGCCTGTGGA
GTCTTAATTCAGGGGGAAACTTCGCATTATGAGCATATTGCGGATAATGTTGCCGCAGGTATCGCTCGATTGTCTGTAGA
ATTCTGTCTCCCAATCACTTTTTCTGTGATTACTGCTCCTAATGTAGAAGCTGCTTGGGAAAGAGCAGGGATAAAAGGCC
CAAATTTGGGAGCTTCAGGAATGAGAACGGCGTTAGAAATGGCGTCGCTGTTTTCATTAATAGAGAAAGAATAA

Upstream 100 bases:

>100_bases
GTCATCGTCTCTTCTGAAAATGAGAGGTATTTACGAACAAAAAAAGACCGAATGGGGCATTGGTTGAATTTCCCTGTTCT
TAATGAGTCGGAGGATGAGT

Downstream 100 bases:

>100_bases
AGATTGATATCGGTGAAAGCGCCCGACTAAAAACCGGGCGCTTTTTTTGTTATAGATGGATGCTGACAGATCCATTCAAG
TGGATTTGAACAGCAACGTT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKLLKGLPVAKDVRVAIVGACFNAPIADRLVSGARETFFESGGSPDSLTVVRVPGAFEIPCAIKKMLSKGNLFQAIVACG
VLIQGETSHYEHIADNVAAGIARLSVEFCLPITFSVITAPNVEAAWERAGIKGPNLGASGMRTALEMASLFSLIEKE

Sequences:

>Translated_157_residues
MKLLKGLPVAKDVRVAIVGACFNAPIADRLVSGARETFFESGGSPDSLTVVRVPGAFEIPCAIKKMLSKGNLFQAIVACG
VLIQGETSHYEHIADNVAAGIARLSVEFCLPITFSVITAPNVEAAWERAGIKGPNLGASGMRTALEMASLFSLIEKE
>Mature_157_residues
MKLLKGLPVAKDVRVAIVGACFNAPIADRLVSGARETFFESGGSPDSLTVVRVPGAFEIPCAIKKMLSKGNLFQAIVACG
VLIQGETSHYEHIADNVAAGIARLSVEFCLPITFSVITAPNVEAAWERAGIKGPNLGASGMRTALEMASLFSLIEKE

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=156, Percent_Identity=37.8205128205128, Blast_Score=100, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324429, Length=147, Percent_Identity=31.9727891156463, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_CHLMU (Q9PLJ4)

Other databases:

- EMBL:   AE002160
- PIR:   C81740
- RefSeq:   NP_296489.1
- ProteinModelPortal:   Q9PLJ4
- SMR:   Q9PLJ4
- GeneID:   1245635
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0105
- TIGR:   TC_0105
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   Q9PLJ4
- ProtClustDB:   PRK00061
- BioCyc:   CMUR243161:TC_0105-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16546; Mature: 16546

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKGLPVAKDVRVAIVGACFNAPIADRLVSGARETFFESGGSPDSLTVVRVPGAFEIP
CCCCCCCCCCCCHHHEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
CAIKKMLSKGNLFQAIVACGVLIQGETSHYEHIADNVAAGIARLSVEFCLPITFSVITAP
HHHHHHHCCCCHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
NVEAAWERAGIKGPNLGASGMRTALEMASLFSLIEKE
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLLKGLPVAKDVRVAIVGACFNAPIADRLVSGARETFFESGGSPDSLTVVRVPGAFEIP
CCCCCCCCCCCCHHHEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
CAIKKMLSKGNLFQAIVACGVLIQGETSHYEHIADNVAAGIARLSVEFCLPITFSVITAP
HHHHHHHCCCCHHHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
NVEAAWERAGIKGPNLGASGMRTALEMASLFSLIEKE
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935