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The map label for this gene is ribA/B

Identifier: 15834729

GI number: 15834729

Start: 123067

End: 124341

Strand: Direct

Name: ribA/B

Synonym: TC0104

Alternate gene names: 15834729

Gene position: 123067-124341 (Clockwise)

Preceding gene: 15834728

Following gene: 15834730

Centisome position: 11.47

GC content: 43.14

Gene sequence:

>1275_bases
ATGGTTACATGTGAAGCCGGGATAGCTTCGGTACAGCAAGCGATAAAAGATATCGCTGACGGGAATTTTGTCATAGTTAT
TGATAGGGAATCTCGAGAGAACGAGGGAGACTTAATTTTAGCAGGAGAAAAGGTCTCGGCAGAAAAAATGGCCTTTCTTT
TGTCTCACACAACGGGCATTGTCTGCGCTTCGGTCACTCGTGAGCAAGCAAGAGTTTTGGACCTGCCCGCAATGGTTCAA
GAGAACCAATGTGCCTTTAAAACAGCTTTTACTGTTTCTGTAGATGCTTCGTCAGGAATTACTACGGGAGTTTCTGCAGC
GGATAGAACGAGAACAGTTCAATTATTATCCGACCCAACGTCAATTTCTCAATCTTTTGTTCGTCCAGGACATGTTTTCC
CTTTAGTTAGTCAGCCTGGTGGAGTAGTCAAACGTCCAGGACATACTGAAGCATCCATGGATCTCATGCGGCTTGCTGGG
ATGTATCCTTGTGGTATTTTTGCTGAGCTTGTCAATGCAGATCATTCTATGATGCGGCAACAACAGATACTAGATTTTGC
TGAGCAACATGGTTTTACGGTTATTACCGTAGATGATCTGATTACTTATCGATGGACCTTTGATTCTTTAGTTGAGCATG
TTTCTTCAGCACGGATTCCTACCAAATATGGGGAGTTCTTCATACATGTTTATAAATCGATTATTGATGGAACAGAGCAT
TTTGCTTTAGTGAAGGGAGATATTCGGGAACAAGAATCCGTGCCTGTGCGTGTGCACTCAGAATGCTTAACCGGAGATGT
TTTGGGGTCTTGTCGTTGTGATTGCGGAGCTCAGTTGGATATGGCTATGCGGTATATTGCAGAACAGGGCTTAGGCGTTA
TTGTGTATCTTCGAGGGCAAGAAGGGCGCGGTATAGGTTTTGGGCATAAAATTCAAGCTTATGCTTTACAAGATCTTGGA
TATGATACGGTAGAGGCCAATCTCCAACTAAGTTTTCCTATAGATGCTCGAGAGTATGGAGTAGCTGCACAAATTCTTAA
GGATCTCCGGTTGACAAGCGTGAGATTGATCACCCATAATCCAAAAAAGTTTTTTGAGCTACAGCGGTTAGGAATACACA
TTCTCGACCGGATTGTTTTGCCCGTCATCGTCTCTTCTGAAAATGAGAGGTATTTACGAACAAAAAAAGACCGAATGGGG
CATTGGTTGAATTTCCCTGTTCTTAATGAGTCGGAGGATGAGTATGAAACTGTTGAAAGGACTTCCTGTTGCTAA

Upstream 100 bases:

>100_bases
GAAAGTATAAGTTTGTGCTATACTCTGCACACAGCAAGGAGCTTGTATTAGAAAAACTTTCCACTAGCTGAGTTTTTCTC
AAAAACGGGATAGATTGAGA

Downstream 100 bases:

>100_bases
GGATGTGCGTGTAGCTATAGTCGGGGCGTGCTTTAACGCTCCTATTGCTGATCGCTTGGTTTCCGGAGCTCGAGAGACAT
TTTTTGAGTCCGGAGGTAGT

Product: bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MVTCEAGIASVQQAIKDIADGNFVIVIDRESRENEGDLILAGEKVSAEKMAFLLSHTTGIVCASVTREQARVLDLPAMVQ
ENQCAFKTAFTVSVDASSGITTGVSAADRTRTVQLLSDPTSISQSFVRPGHVFPLVSQPGGVVKRPGHTEASMDLMRLAG
MYPCGIFAELVNADHSMMRQQQILDFAEQHGFTVITVDDLITYRWTFDSLVEHVSSARIPTKYGEFFIHVYKSIIDGTEH
FALVKGDIREQESVPVRVHSECLTGDVLGSCRCDCGAQLDMAMRYIAEQGLGVIVYLRGQEGRGIGFGHKIQAYALQDLG
YDTVEANLQLSFPIDAREYGVAAQILKDLRLTSVRLITHNPKKFFELQRLGIHILDRIVLPVIVSSENERYLRTKKDRMG
HWLNFPVLNESEDEYETVERTSCC

Sequences:

>Translated_424_residues
MVTCEAGIASVQQAIKDIADGNFVIVIDRESRENEGDLILAGEKVSAEKMAFLLSHTTGIVCASVTREQARVLDLPAMVQ
ENQCAFKTAFTVSVDASSGITTGVSAADRTRTVQLLSDPTSISQSFVRPGHVFPLVSQPGGVVKRPGHTEASMDLMRLAG
MYPCGIFAELVNADHSMMRQQQILDFAEQHGFTVITVDDLITYRWTFDSLVEHVSSARIPTKYGEFFIHVYKSIIDGTEH
FALVKGDIREQESVPVRVHSECLTGDVLGSCRCDCGAQLDMAMRYIAEQGLGVIVYLRGQEGRGIGFGHKIQAYALQDLG
YDTVEANLQLSFPIDAREYGVAAQILKDLRLTSVRLITHNPKKFFELQRLGIHILDRIVLPVIVSSENERYLRTKKDRMG
HWLNFPVLNESEDEYETVERTSCC
>Mature_424_residues
MVTCEAGIASVQQAIKDIADGNFVIVIDRESRENEGDLILAGEKVSAEKMAFLLSHTTGIVCASVTREQARVLDLPAMVQ
ENQCAFKTAFTVSVDASSGITTGVSAADRTRTVQLLSDPTSISQSFVRPGHVFPLVSQPGGVVKRPGHTEASMDLMRLAG
MYPCGIFAELVNADHSMMRQQQILDFAEQHGFTVITVDDLITYRWTFDSLVEHVSSARIPTKYGEFFIHVYKSIIDGTEH
FALVKGDIREQESVPVRVHSECLTGDVLGSCRCDCGAQLDMAMRYIAEQGLGVIVYLRGQEGRGIGFGHKIQAYALQDLG
YDTVEANLQLSFPIDAREYGVAAQILKDLRLTSVRLITHNPKKFFELQRLGIHILDRIVLPVIVSSENERYLRTKKDRMG
HWLNFPVLNESEDEYETVERTSCC

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate

COG id: COG0807

COG function: function code H; GTP cyclohydrolase II

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family

Homologues:

Organism=Escherichia coli, GI1789420, Length=194, Percent_Identity=45.8762886597938, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787533, Length=194, Percent_Identity=50.5154639175258, Blast_Score=191, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6320695, Length=201, Percent_Identity=43.7810945273632, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6319438, Length=186, Percent_Identity=46.7741935483871, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBBA_CHLMU (Q9PLJ5)

Other databases:

- EMBL:   AE002160
- PIR:   B81740
- RefSeq:   NP_296488.1
- ProteinModelPortal:   Q9PLJ5
- GeneID:   1245634
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0104
- TIGR:   TC_0104
- HOGENOM:   HBG735778
- OMA:   RCDCRMQ
- PhylomeDB:   Q9PLJ5
- ProtClustDB:   PRK09311
- BioCyc:   CMUR243161:TC_0104-MONOMER
- BRENDA:   3.5.4.25
- BRENDA:   4.1.99.12
- HAMAP:   MF_01283
- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299
- Gene3D:   G3DSA:3.90.870.10
- PIRSF:   PIRSF001259
- TIGRFAMs:   TIGR00505
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12; =3.5.4.25

Molecular weight: Translated: 47067; Mature: 47067

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: ACT_SITE 335-335 ACT_SITE 337-337 BINDING 37-37 BINDING 169-169 BINDING 278-278 BINDING 323-323 BINDING 358-358 BINDING 363-363

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTCEAGIASVQQAIKDIADGNFVIVIDRESRENEGDLILAGEKVSAEKMAFLLSHTTGI
CEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCCE
VCASVTREQARVLDLPAMVQENQCAFKTAFTVSVDASSGITTGVSAADRTRTVQLLSDPT
EEECCCHHHHHHHCCHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCH
SISQSFVRPGHVFPLVSQPGGVVKRPGHTEASMDLMRLAGMYPCGIFAELVNADHSMMRQ
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHH
QQILDFAEQHGFTVITVDDLITYRWTFDSLVEHVSSARIPTKYGEFFIHVYKSIIDGTEH
HHHHHHHHHCCCEEEEHHHCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
FALVKGDIREQESVPVRVHSECLTGDVLGSCRCDCGAQLDMAMRYIAEQGLGVIVYLRGQ
EEEEECCCCCCCCCCEEEECHHHCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
EGRGIGFGHKIQAYALQDLGYDTVEANLQLSFPIDAREYGVAAQILKDLRLTSVRLITHN
CCCCCCCCCEEEEEEHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEEECC
PKKFFELQRLGIHILDRIVLPVIVSSENERYLRTKKDRMGHWLNFPVLNESEDEYETVER
HHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCEECCCCCCCCCHHHHHHHH
TSCC
HCCC
>Mature Secondary Structure
MVTCEAGIASVQQAIKDIADGNFVIVIDRESRENEGDLILAGEKVSAEKMAFLLSHTTGI
CEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCCE
VCASVTREQARVLDLPAMVQENQCAFKTAFTVSVDASSGITTGVSAADRTRTVQLLSDPT
EEECCCHHHHHHHCCHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCH
SISQSFVRPGHVFPLVSQPGGVVKRPGHTEASMDLMRLAGMYPCGIFAELVNADHSMMRQ
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHH
QQILDFAEQHGFTVITVDDLITYRWTFDSLVEHVSSARIPTKYGEFFIHVYKSIIDGTEH
HHHHHHHHHCCCEEEEHHHCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
FALVKGDIREQESVPVRVHSECLTGDVLGSCRCDCGAQLDMAMRYIAEQGLGVIVYLRGQ
EEEEECCCCCCCCCCEEEECHHHCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECC
EGRGIGFGHKIQAYALQDLGYDTVEANLQLSFPIDAREYGVAAQILKDLRLTSVRLITHN
CCCCCCCCCEEEEEEHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEEECC
PKKFFELQRLGIHILDRIVLPVIVSSENERYLRTKKDRMGHWLNFPVLNESEDEYETVER
HHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCEECCCCCCCCCHHHHHHHH
TSCC
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10684935