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The map label for this gene is gyrA-1

Identifier: 15834656

GI number: 15834656

Start: 34919

End: 36391

Strand: Reverse

Name: gyrA-1

Synonym: TC0031

Alternate gene names: 15834656

Gene position: 36391-34919 (Counterclockwise)

Preceding gene: 15834657

Following gene: 15834650

Centisome position: 3.39

GC content: 39.78

Gene sequence:

>1473_bases
ATGAGCAATATCTCGGATCTATTTAAGACTCATTTCACACAATACGCGTCTTATGTCATCTTAGAGCGCGCAATCCCCCA
TGTTTTAGATGGACTCAAGCCTGTTCAAAGACGACTCCTTTGGACCTTATTCCGTATGGATGATGGTAAAATGCACAAAG
TTGCCAACATTGCGGGACGAACGATGGCATTACATCCTCATGGCGATGCGCCTATTGTGGAAGCTCTTGTTGTTTTAGCA
AACAAAGGTTTCCTCATAGAAACACAAGGTAATTTTGGCAATCCCCTAACAGGCGATCCACATGCTGCTGCTCGTTATAT
AGAAGCACGATTAAGTCCATTAGCCAAAGAGGTGCTTTTTAACACAGATCTCATGACATTTCATGATTCGTATGATGGAA
GAGAGCAAGAACCAGACATTTTAGCTGCAAAAATTCCTCTATTACTTCTTCATGGAGTGGATGGAATTGCAGTAGGTATG
ACTACAAAAATTTTCCCTCATAATTTTTGTGATTTGCTGAAAGCTCAAATAGCCATACTCAATGACCAACCTTTTTCTCT
TTTCCCGGATTTTGCTTCTGGGGGTTCGATGGATGCCTCGGACTATCAAGACGGATTAGGATCTATCGTTCTTCGCGCAA
CTATAGATATTGTTAACGACAAAACCCTGTTAATCAAAGAGATCTGCCCATCCACAACTACAGAAACTCTTATTCGCTCT
ATTGAAAACGCAGCTAAACGTGGAATTATTAAGATCGATTCTATCCAAGATTTTTCGACAGATCTTCCACATATAGAGAT
TAAACTCCCTAAAGGGGTCCATGCTAAAGATCTATTGCAACCCCTATACGCTCACACAGAATGTCAGGTCGTTTTAACCT
CTCGTCCAACAGCAATCTACCAAGGAAAACCCTGGGAAACTTCTGTAAGCGAAATCCTCCGCTTACAAACAGAAACTCTC
CAAAGCTACCTAAAAAAAGAATTACTTATTTTAGAAGATTCGTTAACTCGCGAGCATTACCACAAAACTCTTGAATACCT
ATTCATCAAACACAAGCTTTATGACACTGTGCGCGCCATGCTTTCTAAGAGAAAAACTTCTCCCTCAGCAAGCGTTATTC
ACAATGCAGTCACAGATGCTTTAGCTCCGTTCTTAGGAACACTCCCTACACCTGATAAACAAGCAACTACACAACTTGCT
TCTTTAACAATCAAAAAAATCCTATGTTTTGATGAAAATGCTTATGAAAAAGAGCTTCTATCCTTAGAGAAAAAGCGCTG
TAACGTGCAAAAAGATCTTGGACAATTGAAAAAATATACGATTCTGTACATCAAAAAACTGCTCGATGCTTACAAGCATC
TCGGACATCGAAAGACAAAAATTGAAAAATTCGAAGACGCGCTTTTAGGAAAATCCATCCATAAAAAAGCAAAAGAAGCA
TCCACCATCGACCAAGAAGAATCTCTGGTCTAA

Upstream 100 bases:

>100_bases
TAGATGCACTTCTACAGTTCTACATGGGGAAAAATACGAAAGAGCGGAAGCACTTTATTATTGAGAATCTAGTTACTAAC
CTATAGCGACAAACACACTT

Downstream 100 bases:

>100_bases
TAAACCCTCGAGGGCCCTAGAGTTCACACCTCCCGGGCCAACAGTGCCTATCGATCTCTTTTTGCAACAAAAAAGCTATC
AAGAGCTCTTATCGCTCTTC

Product: DNA topoisomerase IV subunit A

Products: NA

Alternate protein names: Topoisomerase IV subunit A [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSNISDLFKTHFTQYASYVILERAIPHVLDGLKPVQRRLLWTLFRMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLA
NKGFLIETQGNFGNPLTGDPHAAARYIEARLSPLAKEVLFNTDLMTFHDSYDGREQEPDILAAKIPLLLLHGVDGIAVGM
TTKIFPHNFCDLLKAQIAILNDQPFSLFPDFASGGSMDASDYQDGLGSIVLRATIDIVNDKTLLIKEICPSTTTETLIRS
IENAAKRGIIKIDSIQDFSTDLPHIEIKLPKGVHAKDLLQPLYAHTECQVVLTSRPTAIYQGKPWETSVSEILRLQTETL
QSYLKKELLILEDSLTREHYHKTLEYLFIKHKLYDTVRAMLSKRKTSPSASVIHNAVTDALAPFLGTLPTPDKQATTQLA
SLTIKKILCFDENAYEKELLSLEKKRCNVQKDLGQLKKYTILYIKKLLDAYKHLGHRKTKIEKFEDALLGKSIHKKAKEA
STIDQEESLV

Sequences:

>Translated_490_residues
MSNISDLFKTHFTQYASYVILERAIPHVLDGLKPVQRRLLWTLFRMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLA
NKGFLIETQGNFGNPLTGDPHAAARYIEARLSPLAKEVLFNTDLMTFHDSYDGREQEPDILAAKIPLLLLHGVDGIAVGM
TTKIFPHNFCDLLKAQIAILNDQPFSLFPDFASGGSMDASDYQDGLGSIVLRATIDIVNDKTLLIKEICPSTTTETLIRS
IENAAKRGIIKIDSIQDFSTDLPHIEIKLPKGVHAKDLLQPLYAHTECQVVLTSRPTAIYQGKPWETSVSEILRLQTETL
QSYLKKELLILEDSLTREHYHKTLEYLFIKHKLYDTVRAMLSKRKTSPSASVIHNAVTDALAPFLGTLPTPDKQATTQLA
SLTIKKILCFDENAYEKELLSLEKKRCNVQKDLGQLKKYTILYIKKLLDAYKHLGHRKTKIEKFEDALLGKSIHKKAKEA
STIDQEESLV
>Mature_489_residues
SNISDLFKTHFTQYASYVILERAIPHVLDGLKPVQRRLLWTLFRMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLAN
KGFLIETQGNFGNPLTGDPHAAARYIEARLSPLAKEVLFNTDLMTFHDSYDGREQEPDILAAKIPLLLLHGVDGIAVGMT
TKIFPHNFCDLLKAQIAILNDQPFSLFPDFASGGSMDASDYQDGLGSIVLRATIDIVNDKTLLIKEICPSTTTETLIRSI
ENAAKRGIIKIDSIQDFSTDLPHIEIKLPKGVHAKDLLQPLYAHTECQVVLTSRPTAIYQGKPWETSVSEILRLQTETLQ
SYLKKELLILEDSLTREHYHKTLEYLFIKHKLYDTVRAMLSKRKTSPSASVIHNAVTDALAPFLGTLPTPDKQATTQLAS
LTIKKILCFDENAYEKELLSLEKKRCNVQKDLGQLKKYTILYIKKLLDAYKHLGHRKTKIEKFEDALLGKSIHKKAKEAS
TIDQEESLV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=227, Percent_Identity=29.5154185022026, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI19913408, Length=334, Percent_Identity=27.2455089820359, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788562, Length=341, Percent_Identity=31.9648093841642, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1789396, Length=512, Percent_Identity=26.953125, Blast_Score=136, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=168, Percent_Identity=34.5238095238095, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535065, Length=173, Percent_Identity=31.7919075144509, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI115534553, Length=166, Percent_Identity=29.5180722891566, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI212645657, Length=172, Percent_Identity=29.6511627906977, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=226, Percent_Identity=28.7610619469027, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136538, Length=248, Percent_Identity=30.6451612903226, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760 [H]

Pfam domain/function: PF00521 DNA_topoisoIV [H]

EC number: 5.99.1.3

Molecular weight: Translated: 55000; Mature: 54869

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNISDLFKTHFTQYASYVILERAIPHVLDGLKPVQRRLLWTLFRMDDGKMHKVANIAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
TMALHPHGDAPIVEALVVLANKGFLIETQGNFGNPLTGDPHAAARYIEARLSPLAKEVLF
EEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NTDLMTFHDSYDGREQEPDILAAKIPLLLLHGVDGIAVGMTTKIFPHNFCDLLKAQIAIL
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHC
NDQPFSLFPDFASGGSMDASDYQDGLGSIVLRATIDIVNDKTLLIKEICPSTTTETLIRS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
IENAAKRGIIKIDSIQDFSTDLPHIEIKLPKGVHAKDLLQPLYAHTECQVVLTSRPTAIY
HHHHHHCCCEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCEEE
QGKPWETSVSEILRLQTETLQSYLKKELLILEDSLTREHYHKTLEYLFIKHKLYDTVRAM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKRKTSPSASVIHNAVTDALAPFLGTLPTPDKQATTQLASLTIKKILCFDENAYEKELL
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SLEKKRCNVQKDLGQLKKYTILYIKKLLDAYKHLGHRKTKIEKFEDALLGKSIHKKAKEA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
STIDQEESLV
HCCCHHHCCC
>Mature Secondary Structure 
SNISDLFKTHFTQYASYVILERAIPHVLDGLKPVQRRLLWTLFRMDDGKMHKVANIAGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
TMALHPHGDAPIVEALVVLANKGFLIETQGNFGNPLTGDPHAAARYIEARLSPLAKEVLF
EEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NTDLMTFHDSYDGREQEPDILAAKIPLLLLHGVDGIAVGMTTKIFPHNFCDLLKAQIAIL
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHC
NDQPFSLFPDFASGGSMDASDYQDGLGSIVLRATIDIVNDKTLLIKEICPSTTTETLIRS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
IENAAKRGIIKIDSIQDFSTDLPHIEIKLPKGVHAKDLLQPLYAHTECQVVLTSRPTAIY
HHHHHHCCCEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCEEE
QGKPWETSVSEILRLQTETLQSYLKKELLILEDSLTREHYHKTLEYLFIKHKLYDTVRAM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKRKTSPSASVIHNAVTDALAPFLGTLPTPDKQATTQLASLTIKKILCFDENAYEKELL
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SLEKKRCNVQKDLGQLKKYTILYIKKLLDAYKHLGHRKTKIEKFEDALLGKSIHKKAKEA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
STIDQEESLV
HCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]