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The map label for this gene is kdsA

Identifier: 15834650

GI number: 15834650

Start: 31534

End: 32343

Strand: Reverse

Name: kdsA

Synonym: TC0025

Alternate gene names: 15834650

Gene position: 32343-31534 (Counterclockwise)

Preceding gene: 15834656

Following gene: 15834649

Centisome position: 3.01

GC content: 41.11

Gene sequence:

>810_bases
ATGTTCCCAAACAACAAAATGCTCTTGATAGCTGGACCTTGTGTGATTGAGGACAATTCTGTTTTTGAAACCGCACGAAG
ATTAAAAGAAATTGTTGCTCCCTATTCTTCTTCTGTTCATTGGATCTTTAAAAGCAGTTATGACAAAGCGAACCGCTCGT
CTTTGCAAAATTATCGCGGTCCTGGGTTGAAATTAGGGTTACAAACATTAGCTAAAATTAAAGAAACTTTCGATGTGGAG
ATTTTAACAGATGTGCATTCTCCTGACGAGGCTCGAGAGGCTGCTAAAGTCTGTGATATTATTCAAGTTCCGGCTTTTTT
GTGCCGCCAAACAGATCTCCTTGTTACCGCAGGAGAAACTCAAGCTATCGTGAATATTAAAAAGGGACAATTTCTTTCTC
CTTGGGAAATGCAAGGACCTATTGATAAAGTCCTCTCTACCGGGAATAACAAAATTATCTTAACCGAAAGAGGGTGCTCT
TTCGGTTACAATAATCTTGTGTCCGATATGCGCTCTATCGAAGTTCTCCGGCGCTTTGGTTTTCCTGTTATTTTTGACGG
CACACATTCCATACAACTACCAGGGGCTTTACAAAGTCAAAGTGGTGGACAAACCGAGTTTATTCCTGTCCTAACGCGTT
CTGCTATAGCCGCAGGCGTACATGGATTGTTTATAGAAACACATCCTAATCCTGCTTCCGCTCTAAGTGATGCCGCTTCT
ATGCTCTCTTTGAAAGATCTAGAACGATTATTACCATCTTGGGTACAACTGTTCACATACATTCAAGAGATGGACACCGT
TTCTATATGA

Upstream 100 bases:

>100_bases
CAAACGTAAATGGTGTATAGTCGAGTTTCTGTCAGCTACGAATATCTTTAGAGCCCTGACTAAAAGCGCAATTTCACTCT
AAAACAAAGGATCACCCTTT

Downstream 100 bases:

>100_bases
CAAAGTTTCTTTTTCACGGCATTTGGTGTACAGTCGTCCTAGTATTATGTGCTTGTATAACAGCGTTAGCTGTTGTCAAA
ATGGGAAATTTCACGAATCC

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MFPNNKMLLIAGPCVIEDNSVFETARRLKEIVAPYSSSVHWIFKSSYDKANRSSLQNYRGPGLKLGLQTLAKIKETFDVE
ILTDVHSPDEAREAAKVCDIIQVPAFLCRQTDLLVTAGETQAIVNIKKGQFLSPWEMQGPIDKVLSTGNNKIILTERGCS
FGYNNLVSDMRSIEVLRRFGFPVIFDGTHSIQLPGALQSQSGGQTEFIPVLTRSAIAAGVHGLFIETHPNPASALSDAAS
MLSLKDLERLLPSWVQLFTYIQEMDTVSI

Sequences:

>Translated_269_residues
MFPNNKMLLIAGPCVIEDNSVFETARRLKEIVAPYSSSVHWIFKSSYDKANRSSLQNYRGPGLKLGLQTLAKIKETFDVE
ILTDVHSPDEAREAAKVCDIIQVPAFLCRQTDLLVTAGETQAIVNIKKGQFLSPWEMQGPIDKVLSTGNNKIILTERGCS
FGYNNLVSDMRSIEVLRRFGFPVIFDGTHSIQLPGALQSQSGGQTEFIPVLTRSAIAAGVHGLFIETHPNPASALSDAAS
MLSLKDLERLLPSWVQLFTYIQEMDTVSI
>Mature_269_residues
MFPNNKMLLIAGPCVIEDNSVFETARRLKEIVAPYSSSVHWIFKSSYDKANRSSLQNYRGPGLKLGLQTLAKIKETFDVE
ILTDVHSPDEAREAAKVCDIIQVPAFLCRQTDLLVTAGETQAIVNIKKGQFLSPWEMQGPIDKVLSTGNNKIILTERGCS
FGYNNLVSDMRSIEVLRRFGFPVIFDGTHSIQLPGALQSQSGGQTEFIPVLTRSAIAAGVHGLFIETHPNPASALSDAAS
MLSLKDLERLLPSWVQLFTYIQEMDTVSI

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=234, Percent_Identity=44.4444444444444, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_CHLMU (Q9PLS0)

Other databases:

- EMBL:   AE002160
- PIR:   E81747
- RefSeq:   NP_296409.1
- ProteinModelPortal:   Q9PLS0
- SMR:   Q9PLS0
- GeneID:   1245549
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0025
- TIGR:   TC_0025
- HOGENOM:   HBG575990
- OMA:   RSLPIMA
- PhylomeDB:   Q9PLS0
- ProtClustDB:   PRK05198
- BioCyc:   CMUR243161:TC_0025-MONOMER
- BRENDA:   2.5.1.55
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29701; Mature: 29701

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPNNKMLLIAGPCVIEDNSVFETARRLKEIVAPYSSSVHWIFKSSYDKANRSSLQNYRG
CCCCCCEEEEECCEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCC
PGLKLGLQTLAKIKETFDVEILTDVHSPDEAREAAKVCDIIQVPAFLCRQTDLLVTAGET
CCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
QAIVNIKKGQFLSPWEMQGPIDKVLSTGNNKIILTERGCSFGYNNLVSDMRSIEVLRRFG
EEEEEECCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
FPVIFDGTHSIQLPGALQSQSGGQTEFIPVLTRSAIAAGVHGLFIETHPNPASALSDAAS
CCEEECCCCCEECCCCCCCCCCCCEEHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
MLSLKDLERLLPSWVQLFTYIQEMDTVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFPNNKMLLIAGPCVIEDNSVFETARRLKEIVAPYSSSVHWIFKSSYDKANRSSLQNYRG
CCCCCCEEEEECCEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCC
PGLKLGLQTLAKIKETFDVEILTDVHSPDEAREAAKVCDIIQVPAFLCRQTDLLVTAGET
CCHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
QAIVNIKKGQFLSPWEMQGPIDKVLSTGNNKIILTERGCSFGYNNLVSDMRSIEVLRRFG
EEEEEECCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
FPVIFDGTHSIQLPGALQSQSGGQTEFIPVLTRSAIAAGVHGLFIETHPNPASALSDAAS
CCEEECCCCCEECCCCCCCCCCCCEEHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHH
MLSLKDLERLLPSWVQLFTYIQEMDTVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935