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The map label for this gene is hemB

Identifier: 15834626

GI number: 15834626

Start: 266

End: 1267

Strand: Direct

Name: hemB

Synonym: TC0001

Alternate gene names: 15834626

Gene position: 266-1267 (Clockwise)

Preceding gene: NA

Following gene: 15834629

Centisome position: 0.02

GC content: 40.12

Gene sequence:

>1002_bases
ATGACAAGACTTCCTCTACTCAAACGACCTCGAAGAAATCGGAAAAGTGCTGCTATTCGATCTATGATTCGAGAAACTAA
TATGGTTTCAAGTGATCTAATTTGGCCTATTTTTCTTAAAGAAGGTTCAGGAATTCGAGAAGAAATACCCAGTATGCCTG
GAGTATACAGATGGAGTTTAGATACCATATCTAGAGAATTAGAAAGACTCTGTCTTATAGGGCTAAAAGCGGTTATCCTT
TTCCCTGTTATAGAGGATCAGAAAAAAGATCAATTCGGAGCATATGCATCGCATCCGTATAACATTGTTTGTAGAGGAAT
TCAGGAAATCAAAAAATCTTTTCCCCAACTGTGTGTGATTAGTGATATAGCTTTAGATCCTTTTACGACTAGTGGTCACG
ATGGCATTTTTTACAACAATGAAGTGTTAAATGACGAAAGCGTTCGCGTATATGGAGATATCGCAACATTGCATGCAGAA
ATGGGAGCGGATATTGTTGCTCCAAGCGATATGATGGATGGGAGAGTTCGGCATATTCGAGAAAAAATGGATCAGATGGG
CTTTGTTAATACAGGAATTCTCTCTTATAGCGCAAAGTATGCATCTTACTTGTATGGTCCGTTTAGAGATGCTCTTTCTT
CGCATCCTCAATCTGGGGATAAACGGCAATATCAAATGGATCCTGCCAATGTTCGAGAAGCTTTGCTTGAATGCCGGCTA
GATGAAGAGGAGGGAGCAGATATGGTAATGATAAAGCCTGCTGGGTTTTATTTAGATGTTATCATGAAAGCTCAAGAATG
TACGCATCTTCCAGTAGTTGCCTATCAAGTCAGTGGAGAATATTCTATGATTATGGCCGCCAGTCTGCATGGATGGTTAA
GTAAAGAAGGAGCTATCTCGGAGTCTTTGTTAGCAATTAAACGAGCTGGGGCTACGGCTATAATTAGCTATGCAACTCCA
TGGGTTTTAGAATGGCTAGCTAGAGACGCTCTTCCTTTTTAA

Upstream 100 bases:

>100_bases
TATAAACATTTTCCACACCACCTAAAGAAGAAGAGACTAATAAATATCTCTAAGAATCTTCTTCTGTGGGTGTGGAAACT
TTCGATAAGTGTGAAACTTT

Downstream 100 bases:

>100_bases
GGAAAAAGTTCTTTGAAGAACCACCTAAAAAATTACAGAGGTGTCAAAACATTTTCTTTCGCGTAACGGATGAGAGCTTC
TTTTACAATAGCAAACATCT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTRLPLLKRPRRNRKSAAIRSMIRETNMVSSDLIWPIFLKEGSGIREEIPSMPGVYRWSLDTISRELERLCLIGLKAVIL
FPVIEDQKKDQFGAYASHPYNIVCRGIQEIKKSFPQLCVISDIALDPFTTSGHDGIFYNNEVLNDESVRVYGDIATLHAE
MGADIVAPSDMMDGRVRHIREKMDQMGFVNTGILSYSAKYASYLYGPFRDALSSHPQSGDKRQYQMDPANVREALLECRL
DEEEGADMVMIKPAGFYLDVIMKAQECTHLPVVAYQVSGEYSMIMAASLHGWLSKEGAISESLLAIKRAGATAIISYATP
WVLEWLARDALPF

Sequences:

>Translated_333_residues
MTRLPLLKRPRRNRKSAAIRSMIRETNMVSSDLIWPIFLKEGSGIREEIPSMPGVYRWSLDTISRELERLCLIGLKAVIL
FPVIEDQKKDQFGAYASHPYNIVCRGIQEIKKSFPQLCVISDIALDPFTTSGHDGIFYNNEVLNDESVRVYGDIATLHAE
MGADIVAPSDMMDGRVRHIREKMDQMGFVNTGILSYSAKYASYLYGPFRDALSSHPQSGDKRQYQMDPANVREALLECRL
DEEEGADMVMIKPAGFYLDVIMKAQECTHLPVVAYQVSGEYSMIMAASLHGWLSKEGAISESLLAIKRAGATAIISYATP
WVLEWLARDALPF
>Mature_332_residues
TRLPLLKRPRRNRKSAAIRSMIRETNMVSSDLIWPIFLKEGSGIREEIPSMPGVYRWSLDTISRELERLCLIGLKAVILF
PVIEDQKKDQFGAYASHPYNIVCRGIQEIKKSFPQLCVISDIALDPFTTSGHDGIFYNNEVLNDESVRVYGDIATLHAEM
GADIVAPSDMMDGRVRHIREKMDQMGFVNTGILSYSAKYASYLYGPFRDALSSHPQSGDKRQYQMDPANVREALLECRLD
EEEGADMVMIKPAGFYLDVIMKAQECTHLPVVAYQVSGEYSMIMAASLHGWLSKEGAISESLLAIKRAGATAIISYATPW
VLEWLARDALPF

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=38.6363636363636, Blast_Score=214, Evalue=9e-56,
Organism=Escherichia coli, GI87081728, Length=312, Percent_Identity=43.5897435897436, Blast_Score=249, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321398, Length=313, Percent_Identity=39.297124600639, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI21358291, Length=298, Percent_Identity=34.8993288590604, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM2_CHLMU (Q9PLU4)

Other databases:

- EMBL:   AE002160
- PIR:   B81751
- RefSeq:   NP_296385.1
- ProteinModelPortal:   Q9PLU4
- SMR:   Q9PLU4
- GeneID:   1245524
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0001
- TIGR:   TC_0001
- HOGENOM:   HBG285270
- OMA:   FTSHGHD
- PhylomeDB:   Q9PLU4
- ProtClustDB:   PRK09283
- BioCyc:   CMUR243161:TC_0001-MONOMER
- BRENDA:   4.2.1.24
- InterPro:   IPR001731
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11458
- PIRSF:   PIRSF001415
- PRINTS:   PR00144

Pfam domain/function: PF00490 ALAD

EC number: =4.2.1.24

Molecular weight: Translated: 37451; Mature: 37319

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: ACT_SITE 199-199 ACT_SITE 252-252 BINDING 209-209 BINDING 221-221 BINDING 278-278 BINDING 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLPLLKRPRRNRKSAAIRSMIRETNMVSSDLIWPIFLKEGSGIREEIPSMPGVYRWSL
CCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCEEEEEEECCCCHHHHCCCCCCEEEEEH
DTISRELERLCLIGLKAVILFPVIEDQKKDQFGAYASHPYNIVCRGIQEIKKSFPQLCVI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
SDIALDPFTTSGHDGIFYNNEVLNDESVRVYGDIATLHAEMGADIVAPSDMMDGRVRHIR
HHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHCCCEECCCHHHHHHHHHHH
EKMDQMGFVNTGILSYSAKYASYLYGPFRDALSSHPQSGDKRQYQMDPANVREALLECRL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCC
DEEEGADMVMIKPAGFYLDVIMKAQECTHLPVVAYQVSGEYSMIMAASLHGWLSKEGAIS
CCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCHH
ESLLAIKRAGATAIISYATPWVLEWLARDALPF
HHHHHHHHCCCEEEEHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRLPLLKRPRRNRKSAAIRSMIRETNMVSSDLIWPIFLKEGSGIREEIPSMPGVYRWSL
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCEEEEEEECCCCHHHHCCCCCCEEEEEH
DTISRELERLCLIGLKAVILFPVIEDQKKDQFGAYASHPYNIVCRGIQEIKKSFPQLCVI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
SDIALDPFTTSGHDGIFYNNEVLNDESVRVYGDIATLHAEMGADIVAPSDMMDGRVRHIR
HHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHCCCEECCCHHHHHHHHHHH
EKMDQMGFVNTGILSYSAKYASYLYGPFRDALSSHPQSGDKRQYQMDPANVREALLECRL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCC
DEEEGADMVMIKPAGFYLDVIMKAQECTHLPVVAYQVSGEYSMIMAASLHGWLSKEGAIS
CCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCHH
ESLLAIKRAGATAIISYATPWVLEWLARDALPF
HHHHHHHHCCCEEEEHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935