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The map label for this gene is tfbE

Identifier: 16120279

GI number: 16120279

Start: 307399

End: 308322

Strand: Reverse

Name: tfbE

Synonym: VNG6389G

Alternate gene names: 16120279

Gene position: 308322-307399 (Counterclockwise)

Preceding gene: 16120280

Following gene: 16120278

Centisome position: 84.37

GC content: 66.13

Gene sequence:

>924_bases
ATGACGACGAGTGAACCCTACGAGCGTGCGTTCGACGAAGACATCCAGCGTGGTACAACTGAGCCGTGTCCCGAGTGTGG
GGGCCCGGTGCGAACGAATTCAGCCGAAACAGTTTGTGCAGATTGCGGACTCATCATCGACGAGCAGTCGATCGACCGTG
GACCGGAGTGGCACCGCGACGACGCCGATACAGCAAAGCGAACGGGTGCACCACTCACCCCGGCCCGACACGACCGCGGA
CTGTCGACGGTGATCGGCAGCGGACAGGACGCGAGGGGGACTGCCCTCTCGAGCGAGAAGCGTCGACGCCTCGCGCGGAT
GCGTCGCGAACAGTCCCGTGGCCGATGGCGGTCCACGAAGGAGCGCAACCTCGGGCATGGACTCACGGAGATTCGGCGGA
TCGCGAGCGCACTCGGCCTCGCGGATTCAGTGCGTGATCAAGCCTGCCAGCTGTTCCGGACGGCACAGAACGAACGACTG
CTCAAAGGCCGATCAATCGAGGCGATGGCGGCCGCGAGCGTCTTCGGCGCCTGCCGGTGCAACGGGGAGTCGTGGCTCAT
CGACGATGTGGCGCCGATGGCGCAGGTCGGACAGAACCGCGTCGAGAACGCCTACACGGTGCTCAACGAAGAGTTGGGGC
TGCCGACCCCACCAGTCAGCCTGGACCAGTTCGTCCCGCGACTCGCGTCCGACCTCGGCTGCACGGACGTTGTTCGACGC
CGGGCAGAGGAGCTCGTTGTGCAGGCCGTCGACGCCGGCATCACGACCGGCGTGCATCCATCCGGATTCGCCGCCGCCTG
CCTGTACATGGCGGCGTGTGTCCACGACGCGCCGTTGACGCAAGCCGATGCCGCGGCGGCCGCCGGGGTGACCGTCGAGA
CGATCAGGAGCCATCGCGATCGGCTGCTCTCCATCGTAGAATAG

Upstream 100 bases:

>100_bases
GCACCGATCGATCGGGCGTGCGTCTCCCGCCGAGTTTGTCACCCCCCGAAGAGGGGTGCGGGGCGCGCCGAATCGGCGTG
ACCTCAATCAGGTGAGACCG

Downstream 100 bases:

>100_bases
AGAGCTCGTATAGCCGTTAGGTTGCCCGTCAAGCCCATATATGAGTGTGGTAAAGTAATGGGTAGAGAAAGTATGAGTGA
ATCAGAGTCTGAAAAAGTCG

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 5

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTTSEPYERAFDEDIQRGTTEPCPECGGPVRTNSAETVCADCGLIIDEQSIDRGPEWHRDDADTAKRTGAPLTPARHDRG
LSTVIGSGQDARGTALSSEKRRRLARMRREQSRGRWRSTKERNLGHGLTEIRRIASALGLADSVRDQACQLFRTAQNERL
LKGRSIEAMAAASVFGACRCNGESWLIDDVAPMAQVGQNRVENAYTVLNEELGLPTPPVSLDQFVPRLASDLGCTDVVRR
RAEELVVQAVDAGITTGVHPSGFAAACLYMAACVHDAPLTQADAAAAAGVTVETIRSHRDRLLSIVE

Sequences:

>Translated_307_residues
MTTSEPYERAFDEDIQRGTTEPCPECGGPVRTNSAETVCADCGLIIDEQSIDRGPEWHRDDADTAKRTGAPLTPARHDRG
LSTVIGSGQDARGTALSSEKRRRLARMRREQSRGRWRSTKERNLGHGLTEIRRIASALGLADSVRDQACQLFRTAQNERL
LKGRSIEAMAAASVFGACRCNGESWLIDDVAPMAQVGQNRVENAYTVLNEELGLPTPPVSLDQFVPRLASDLGCTDVVRR
RAEELVVQAVDAGITTGVHPSGFAAACLYMAACVHDAPLTQADAAAAAGVTVETIRSHRDRLLSIVE
>Mature_306_residues
TTSEPYERAFDEDIQRGTTEPCPECGGPVRTNSAETVCADCGLIIDEQSIDRGPEWHRDDADTAKRTGAPLTPARHDRGL
STVIGSGQDARGTALSSEKRRRLARMRREQSRGRWRSTKERNLGHGLTEIRRIASALGLADSVRDQACQLFRTAQNERLL
KGRSIEAMAAASVFGACRCNGESWLIDDVAPMAQVGQNRVENAYTVLNEELGLPTPPVSLDQFVPRLASDLGCTDVVRRR
AEELVVQAVDAGITTGVHPSGFAAACLYMAACVHDAPLTQADAAAAAGVTVETIRSHRDRLLSIVE

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=271, Percent_Identity=26.9372693726937, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17564892, Length=245, Percent_Identity=23.265306122449, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325343, Length=313, Percent_Identity=23.961661341853, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17136758, Length=268, Percent_Identity=27.2388059701493, Blast_Score=104, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B5_HALSA (Q9HHH8)

Other databases:

- EMBL:   AE004438
- RefSeq:   NP_395867.1
- ProteinModelPortal:   Q9HHH8
- GeneID:   1449377
- GenomeReviews:   AE004438_GR
- KEGG:   hal:VNG6389G
- NMPDR:   fig|64091.1.peg.2551
- HOGENOM:   HBG646536
- OMA:   DTIPSAC
- ProtClustDB:   CLSK383248
- BioCyc:   HSP64091:VNG6389G-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 33265; Mature: 33133

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSEPYERAFDEDIQRGTTEPCPECGGPVRTNSAETVCADCGLIIDEQSIDRGPEWHRD
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCHHCCCCCCCCCC
DADTAKRTGAPLTPARHDRGLSTVIGSGQDARGTALSSEKRRRLARMRREQSRGRWRSTK
CHHHHHHCCCCCCCCHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHCCHH
ERNLGHGLTEIRRIASALGLADSVRDQACQLFRTAQNERLLKGRSIEAMAAASVFGACRC
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEE
NGESWLIDDVAPMAQVGQNRVENAYTVLNEELGLPTPPVSLDQFVPRLASDLGCTDVVRR
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
RAEELVVQAVDAGITTGVHPSGFAAACLYMAACVHDAPLTQADAAAAAGVTVETIRSHRD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHH
RLLSIVE
HHHHHCC
>Mature Secondary Structure 
TTSEPYERAFDEDIQRGTTEPCPECGGPVRTNSAETVCADCGLIIDEQSIDRGPEWHRD
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCHHCCCCCCCCCC
DADTAKRTGAPLTPARHDRGLSTVIGSGQDARGTALSSEKRRRLARMRREQSRGRWRSTK
CHHHHHHCCCCCCCCHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHCCHH
ERNLGHGLTEIRRIASALGLADSVRDQACQLFRTAQNERLLKGRSIEAMAAASVFGACRC
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEE
NGESWLIDDVAPMAQVGQNRVENAYTVLNEELGLPTPPVSLDQFVPRLASDLGCTDVVRR
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
RAEELVVQAVDAGITTGVHPSGFAAACLYMAACVHDAPLTQADAAAAAGVTVETIRSHRD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHH
RLLSIVE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11016950